GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Ampelograpic and genetic characterisation of grapevine genetic resources from Ozalj-Vivodina region (Croatia)

Ampelograpic and genetic characterisation of grapevine genetic resources from Ozalj-Vivodina region (Croatia)

Abstract

Context and purpose of the study– Ozalj- vivodina region is small vine growing area (only about 100 hectares of vineyards), but with significant number of old, ancient vineyards planted between 50 and 100 years ago. Trend of abandoning or replanting ancient vineyards takes place for the last 30 years. This trend results in grapevine germplasm erosion because traditional varieties are replaced with well known international varieties.Few known traditional varieties are dominantly present in ancient vineyards together with many others of unknown identity. Historical data about prevalence and characteristic of varieties on this area are very poor. For this reason, we started a project with the purpose of identification, characterization and conservation of grapevine germplasm in this area.

Material and methods – Three years study (2016-2018) included ampelographic inventarization of ancient or abandoned vineyards in Ozalj-Vivodina area. A total of 61 samples (vines) were selected for further research and identification. Identification in situ include ampelographic description by standard set of OIV (Organization Internationale de la Vigne et du Vin ) descriptors. Genetic identification was performed using nine microsatellites markers recommended by the European project GRAPEGEN06. Genetic profile of samples was compared by national and several international databases for possible matching between profiles or with other varieties.

Results – Based on microsatellite analysis of the 61 samples, 45 different genotypes were detected which were identified as follows: 18 genotypes did not match with any of the varieties from available databases; 6 genotypes were identified as traditional or native varieties from NW Croatia (Plavec žuti, Kozjak bijeli, Dišeća Ranina, Moslavac (Furmint), Plemenka (Chasselas rouge), Graševina (Welschriesling); 8 genotypes were identified as rare autochthonous Croatian varieties from other wine regions; 7 genotypes represent common varieties from other European countries (Chardonnay, Pinot Blanc, Blaufraenkisch, Sauvignon Blanc, Rkatsiteli, Pamid, Chauch blanc; 5 samples represent a rare variety identified in other European countries (for example Gaensfuesser blau) and one genotype was identified as Belina starohrvatska (syn. Gouais Blanc). It is interesting that Gouais blanc was represented with six samples from five different locations even though it was not considered to be a traditional cultivar in this area. Ampelographic study shown that dominant genotypes have white coloured berry (33), followed by red (7) and rouge (2). Three genotypes had no clusters available during research. Three genotypes have specific muscat flavour and two have a female type of flower. This research shows that Ozalj-Vivodina as a small winegrowing area has rich grapevine germplasm preserved.

DOI:

Publication date: March 11, 2024

Issue: GiESCO 2019

Type: Poster

Authors

Domagoj STUPIĆ1*, Željko ANDABAKA1, Zvjezdana MARKOVIĆ1, Iva ŠIKUTEN1, Petra ŠTAMBUK2, Darko PREINER1,2, Jasminka KAROGLAN KONTIĆ1,2, Edi MALETIĆ1,2, Nikolina ŠTEDUL3, Maja ŽULJ MIHALJEVIĆ1**

1 Faculty of Agriculture, Svetošimunska cesta 25, 10000, Zagreb, Croatia
2 Centre of Excellence for Biodiversity and Molecular Plant Breeding, Svetošimunska 25, 10000, Zagreb, Croatia
3 Croatia Agriculture and Forestry advisory service, Haulikova 14, 47000, Karlovac, Croatia

Contact the author*

Keywords

Vitis vinifera, grapevine, varieties, genotype, ampelography, genetic identification, microsatellites

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Biodiversity in the vineyard agroecosystem: exploring systemic approaches

Biodiversity conservation and restoration are essential for guarantee the provision of ecosystem services associated to vineyard agroecosystem such as climate regulation trough carbon sequestration and control of pests and diseases. Most of published research dealing with the complexity of the vineyard agroecosystems emphasizes the necessity of innovative approaches, including the integration of information at different temporal and spatial scales and development of systemic analysis based on modelling. A biodiversity survey was conducted in the Franciacorta wine-growing area (Lombardy, Italy), one of the most important Italian wine-growing regions for sparkling wine production, considering a portion of the territory of 112 ha. The area was divided into several Environmental Units (EUs), defined as a whole vineyard or portion of vineyard homogenous in terms of four agronomic characteristics: planting year, planting density, cultivar, and training system. In each EU a set of compartments was identified and characterised by specific variables. The compartments are meteorology, morphology (altitude, slope, aspect, row orientation, and solar irradiance), ecological infrastructures and management. The landscape surrounding EU was also characterised in terms of land-use in a buffer zone of 500 m. For each component a specific methodology was identified and applied. Different statistical approaches were used to evaluate the method to integrate the information related to different compartments within the EU and related to the buffer zone. These approaches were also preliminarily evaluated for their ability to describe the contribution of biodiversity and landscape components to ecosystem services. This methodological exploration provides useful indication for the development of a fully systemic approach to structural and functional biodiversity in vineyard agroecosystems, contributing to promote a multifunctional perspective for the all wine-growing sector.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

How can historical cultivars mitigate the effects of climate change?

IFV, INRAe and the national network “Partenaires de la Sélection Vigne” representing 37 organizations from the different wine regions, have been working increasingly closely over the last 2 decades towards the preservation of the French varietal patrimony. There are approximately 600 patrimonial varieties according to INRAe and SupAgro Montpellier experts, including ancient cultivars (400) and intravarietal crossbreeds obtained since the 19th century. In the context of a drastic reduction in such varieties from the mid 1980’s in favor of mainstream varieties, it was essential to carry out an inventory of old vines and vineyards. INRAe Vassal collection plays a key role here as it holds the largest diversity available, along with a rich bibliography and herbariums, offering us the opportunity to document and double check the identity of a cultivar, consolidating the expertise of ampelographers. The work is carried out in several stages, from verifying the existence of a variety in a small region, through to rehabilitation. During this session, the authors present the process that leads to the official registration of a variety. After this, IFV selection center takes over to initiate the process of selection and propagation. A specific focus within regions such as the Alps, Champagne and the South-West will provide details of the full procedure. Bia, Bouysselet, Chardonnay rose, Mecle and the aptly named Tardif, are some of the cultivars that have followed this procedure. Furthermore, a recent regulation established by INAO on “varieties of interest for adaptation purposes” might boost uptake by growers. Since 2006, 36 historical cultivars have been registered. Most of these have been neglected in the past due to late maturity, lack of sugar and high titratable acidity at harvest time. Such characteristics are today considered as positive qualities, not only in mitigation of the effects of climate change, but also as an opportunity for restoring diversity…

Influence of climatic conditions on grape composition of Tempranillo in La Mancha DO (Spain)

The aim of this work was to analyze the variability in grape composition of the Tempranillo cultivar related to climatic conditions, in La Mancha Designation of Origin. Grape composition (sugar content, total acidity, pH, malic acid, and total and extractable anthocyanins) recorded during ripening, were analysed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The relationships between grape parameters with climatic variables related to temperature and to water deficits, referring different periods between phenological events along the growing cycle, were evaluated using regression analysis. High variability in grape composition was observed in the period analysed. Total acidity varied between 3.7 and 7.3 gL-1 while malic acid varied between 1.2 and 4 gL-1. The extractable anthocyanins ranged between 526 and 972 mgL-1, and total anthocyanins ranged between 922 and 1388 mgL-1, being the lowest values recorded in the hottest year (2017). Total acidity decreased 0.77 gL-1 for an increase of 100 GDD, while malic acid decrease in 0.42 gL-1 for the same GDD increase, being the period between veraison and harvest the one that seemed to have higher influence on acidity. In addition, it was confirmed that increasing water deficits decreased acidity. Total and extractable anthocyanins increased in about 210 and 105 mgL-1, respectively, with an increase of 100 GDD from veraison to harvest, and the increase in water deficits favour the increase of anthocyanins, both total and extractable anthocyanins. Total and extractable anthocyanins concentration increased in 35 and 22 mgL-1 per an increase of 10 mm in the water deficit. These results can be of interest to understand the potential changes that grapes composition may suffer under future warmer climates.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...