GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Petiole phosphorus concentration is controlled by the rootstock genetic background in grapevine: is this a key for understanding rootstock conferred vigour?

Petiole phosphorus concentration is controlled by the rootstock genetic background in grapevine: is this a key for understanding rootstock conferred vigour?

Abstract

Context and purpose of the study – Grapevine, Vitis vinifera, requires grafting on Phylloxera tolerant rootstocks of American origin in most viticultural areas of the world. The most commonly used species in rootstock creation are V. berlandieri, V. riparia and V. rupestris. Rootstocks not only provide tolerance to Phylloxera but assure the supply of water and mineral nutrients to the scion. The objective of this work was to determine to what extent rootstocks of different parentages alter the mineral composition of petioles of grapevine.

Material and methods – Vitis vinifera cv. Cabernet Sauvignon clone 169 was grafted onto 13 rootstock genotypes and planted in 2015 in an experimental plot named GreffAdapt. The rootstocks were: Riparia Gloire de Montpellier, 101-14MGt, 3309C, 420A, SO4, 44-53M, Gravesac, Freedom, Dog Ridge, 41B, Rupestris du Lot, 1103P et 110R. The concentration of the following 13 mineral elements was determined in the petioles at veraison (berry softening, 14/08/2017): Nitrogen, Phosphorus, Potassium, Sulphur, Magnesium, Calcium, Sodium, Bore, Zinc, Manganese, Iron, Cupper and Aluminium. Four petioles were harvested from near the clusters from 2 plants for each block (n = 4 per rootstock genotype) and were dried (in an oven at 60°C until they reached a constant mass). Nitrogen content was determined using a Leco FP-528 instrument (LECO, St. Joseph, MI, USA). Other element contents were determined by digesting the plant sample with nitric acid and hydrochloric acid in a CEM Mars5 microwave digester (CEM, Matthews, NC, USA), elemental concentration was determined by reading the solutions on an ICP-OES MS 730-ES (Varian, Palo Alto, CA, USA). Cane pruning weight was also measured for each vine.

Results – The parentage of rootstocks has a significant effect on petiole mineral composition. Rootstocks with at least one V. riparia parent reduced the concentration of P and increased the concentration of Mg and S in the petiole of Cabernet Sauvignon.

Conclusions – Rootstocks with a V. riparia parent generally confer low scion vigour and we have shown that they also confer low petiole P concentration; this could suggest that P uptake and use is related to rootstock conferred vigour in grapevine. These results will be discussed in the context of previous work we have undertaken to understand the genetic architecture of root growth traits in grapevine. This is the first study to demonstrate a significant link between the genetic origin of a rootstock genotype and its ability to regulation scion P content.

DOI:

Publication date: March 11, 2024

Issue: GiESCO 2019

Type: Poster

Authors

Antoine GAUTIER1, Sarah Jane COOKSON1, Loïc LAGALLE1, Nathalie OLLAT1 and Elisa MARGUERIT*

1 UMR EGFV, Bordeaux Sciences Agro, INRA, University of Bordeaux, ISVV, 210 Chemin de Leysotte, F-33882 Villenave d’Ornon, France

Contact the author

Keywords

Rootstocks, mineral element, phosphorus, grapevine, Vitis spp.

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Traditional agroforestry vineyards, sources of inspiration for the agroecological transition of viticulture

A unique “terroir” can be found in southern Bolivia, which combines the specific features of climate, topography and altitude of high valleys, with the management of grapevines staked on trees. It is one of the rare remnants of agroforestry viticulture. A survey was carried out among 29 grapegrowers in three valleys, to characterize the structure and management of these vineyards, and identify the services they expect from trees. Farms were small (2.2 ha on average) and 85% of vineyards were less than 1 ha. Viticulture was associated with vegetable, fruit and fodder production, sometimes in the same fields. Molle trees were found in all plots, together with one or two other native tree species. Traditional grapevine varieties such as Negra Criolla, Moscatel de Alejandría and Vicchoqueña were grown with a large range of densities from 1550 to 9500 vines ha-1. From 18 to 30% of them were staked on trees, with 1.2 to 4.9 vines per tree. The management of these vineyards (irrigation, fertilization and grapevine protection) was described, the most particular technical operation being the coordinated pruning of trees and grapevines. Three types of management could be identified in the three valleys. Grapegrowers had a clear idea of the ecosystem services they expected from trees in their vineyards. The main one was protection against climate hazards (hail, frost, flood). Then they expected benefits in terms of pest and disease control, improvement of soil fertility and resulting yield. At last, some producers claimed that tree-staking was quicker and cheaper than conventional trellising. It can be hypothesized then that agroforestry is a promising technique for the agroecological transition of viticulture. Its contribution to the “terroir” of the high valleys of southern Bolivia and its link with the specificities of the wines and spirits produced there remain to be explored.

Understanding graft union formation by using metabolomic and transcriptomic approaches during the first days after grafting in grapevine

Since the arrival of Phyloxera (Daktulosphaira vitifolia) in Europe at the end of the 19th century, grafting has become essential to cultivate Vitis vinifera. Today, grafting provides not only resistance to this aphid, but it used to adapt the cultivars according to the type of soil, environment, or grape production requirements by using a panel of rootstocks. As part of vineyard decline, it is often mentioned the importance of producing quality grafted grapevine to improve vineyard longevity, but, to our knowledge, no study has been able to demonstrate that grafting has a role in this context. However, some scion/rootstock combinations are considered as incompatible due to poor graft union formation and subsequently high plant mortality soon after grafting. In a context of climate change where the creation of new cultivars and rootstocks is at the centre of research, the ability of new cultivars to be grafted is therefore essential. The early identification of graft incompatibility could allow the selection of non-viable plants before planting and would have a beneficial impact on research and development in the nursery sector. For this reason, our studies have focused on the identification of metabolic and transcriptomic markers of poor grafting success during the first days/week after grafting; we have identified some correlations between some specialized metabolites, especially stilbenes, and grafting success, as well as an accumulation of some amino acids in the incompatible combination. The study of the metabolome and the transcriptome allowed us to understand and characterise the processes involved during graft union formation.