GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Optical visualization of embolism spread in drought‐induced leaves: revealing differences across three grapevine genotypes

Optical visualization of embolism spread in drought‐induced leaves: revealing differences across three grapevine genotypes

Abstract

Context and purpose of the study ‐Evaluation of xylem embolism is an important challenge in identifying drought tolerant genotypes within the context of climate change. Visualization methods such as the optical vulnerability technique (Brodribb et al. 2016) has been shown to be a reliable and accessible approach to observe the spread of embolism in dehydrating leaves (Hochberg et al. 2017; Lamarque et al. 2018). In this study we use the optical technique to examine the development of leaf embolism in three grapevine cultivars as a method to characterize their drought‐tolerance strategy.

Material and methods ‐Potted plants of Grenache, Semillon and Syrah were grown outdoors in 2018 under well‐watered conditions. Leaf embolism formation and spread was evaluated in four individuals per genotype by monitoring changes in light transmission through the xylem after the irrigation was cutted‐off. For each plant, a mature leaf was placed on a scanner and imaged every 5 minutes until complete desiccation. Simultaneous measurements of stem water potential (Ystem) were registered using psychrometers properly installed on the main stem. The accuracy of the psychrometers was evaluated by measuring the leaf water potential in adjacent leaves previously bagged with aluminum foil using a Scholander pressure bomb. The stack of images obtained were analyzed using the ImageJ software as described in Lamarque et al. (2018). The percentage of embolism (%emb) was calculated as the cumulative number of embolised pixels normalized to the total number of embolised pixels throughout the dehydration. Finally, the %emb was represented as a function of Ystem and different events were colored using a continuous scale respective to their time of appearance.

Results ‐Embolism formation and spread in the leaves were detected at different times for each cultivar since the beginning of drought. While Grenache showed the first embolism event at around 48 h of desiccation (‐0.48 MPa), Semillon showed its first event after 72 h (‐1.5 MPa). Syrah plants were placed in between the other two genotypes showing the first embolisms at ‐0.68 MPa. The vulnerability curves (%emb vs Ystem) constructed from the data obtained followed a sigmoidal function for all genotypes and showed a great variability between individuals. In spite of this, the time and water potentials at which the main embolisms occurred was significantly different between cultivars where Grenache showed an early cavitation (P50 at ‐1.43 MPa), followed by Syrah (P50 at ‐1.65 MPa) and Semillon (P50 at ‐2.08 Mpa). The optical technique tested in this study revealed genotype differences in the temporal appearance of leaf embolism suggesting a different strategy to tolerate dehydration. 

DOI:

Publication date: June 19, 2020

Issue: GiESCO 2019

Type: Article

Authors

Silvina DAYER (1), Régis BURLETT (2), Laurent LAMARQUE2, Sylvain DELZON2, Gregory GAMBETTA1*

(1) Institut des Sciences de la Vigne et du Vin, Écophysiologie et Génomique Fonctionnelle de la Vigne, UMR 1287, 210 Chemin de Leysotte, F-33140 Villenave-d’Ornon, France
(2) Biodiversité Gènes et Communautés, Institut National de la Recherche Agronomique (INRA), Université Bordeaux, 33610 Cestas, France

Contact the author

Keywords

 Embolism, drought, xylem cavitation, vessels, grapevine

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

Geospatial trends of bioclimatic indexes in the topographically complex region of Barolo DOCG

Barolo DOCG is an economically important wine producing region in Northwest Italy. It is a small region of approximately 70 km2 gross area. The topography is very complex with steep sloped hills ranging in elevation from below 200 m to 550 m. Barolo DOCG wine is made exclusively from the Nebbiolo grape. Bioclimatic indexes are often used in viticulture to gain a better understanding of broader climate trends which can be compared temporally and geographically. These indexes are also used for identifying potential phenological timing, growing region suitability, and potential risks associated with expected climatic changes. Understanding how topography influences bioclimatic indexes can help with understanding of mesoscale climate behaviour leading to improved decision making and risk management strategies. The average monthly maximum and minimum temperatures, the Cool Night Index, the Huglin Index, and the monthly diurnal range (from July to October) were calculated using data from 45 weather stations within a 40 km radius of the Barolo DOCG growing area between the years 1996 and 2019. Linear and multiple regression models were developed using independent variables (elevation, aspect, slope) extracted from a digital elevation model to identify significant relationships. Bioclimatic indexes were then kriged with external drift using independent variables that showed significant relationships with the bioclimatic index using a 100 m resolution grid. The maximum monthly temperatures and the Huglin Index showed consistent significant negative relationships with elevation in all years. The minimum monthly temperatures showed no relationship with elevation but in some months a small but significant relationship was observed with aspect. Due to the lack of a relationship between minimum monthly temperatures and elevation compared to the significant relationship between maximum monthly temperatures and elevation, monthly diurnal range had a negative relationship with elevation.

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…