GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Optical visualization of embolism spread in drought‐induced leaves: revealing differences across three grapevine genotypes

Optical visualization of embolism spread in drought‐induced leaves: revealing differences across three grapevine genotypes

Abstract

Context and purpose of the study ‐Evaluation of xylem embolism is an important challenge in identifying drought tolerant genotypes within the context of climate change. Visualization methods such as the optical vulnerability technique (Brodribb et al. 2016) has been shown to be a reliable and accessible approach to observe the spread of embolism in dehydrating leaves (Hochberg et al. 2017; Lamarque et al. 2018). In this study we use the optical technique to examine the development of leaf embolism in three grapevine cultivars as a method to characterize their drought‐tolerance strategy.

Material and methods ‐Potted plants of Grenache, Semillon and Syrah were grown outdoors in 2018 under well‐watered conditions. Leaf embolism formation and spread was evaluated in four individuals per genotype by monitoring changes in light transmission through the xylem after the irrigation was cutted‐off. For each plant, a mature leaf was placed on a scanner and imaged every 5 minutes until complete desiccation. Simultaneous measurements of stem water potential (Ystem) were registered using psychrometers properly installed on the main stem. The accuracy of the psychrometers was evaluated by measuring the leaf water potential in adjacent leaves previously bagged with aluminum foil using a Scholander pressure bomb. The stack of images obtained were analyzed using the ImageJ software as described in Lamarque et al. (2018). The percentage of embolism (%emb) was calculated as the cumulative number of embolised pixels normalized to the total number of embolised pixels throughout the dehydration. Finally, the %emb was represented as a function of Ystem and different events were colored using a continuous scale respective to their time of appearance.

Results ‐Embolism formation and spread in the leaves were detected at different times for each cultivar since the beginning of drought. While Grenache showed the first embolism event at around 48 h of desiccation (‐0.48 MPa), Semillon showed its first event after 72 h (‐1.5 MPa). Syrah plants were placed in between the other two genotypes showing the first embolisms at ‐0.68 MPa. The vulnerability curves (%emb vs Ystem) constructed from the data obtained followed a sigmoidal function for all genotypes and showed a great variability between individuals. In spite of this, the time and water potentials at which the main embolisms occurred was significantly different between cultivars where Grenache showed an early cavitation (P50 at ‐1.43 MPa), followed by Syrah (P50 at ‐1.65 MPa) and Semillon (P50 at ‐2.08 Mpa). The optical technique tested in this study revealed genotype differences in the temporal appearance of leaf embolism suggesting a different strategy to tolerate dehydration. 

DOI:

Publication date: June 19, 2020

Issue: GiESCO 2019

Type: Article

Authors

Silvina DAYER (1), Régis BURLETT (2), Laurent LAMARQUE2, Sylvain DELZON2, Gregory GAMBETTA1*

(1) Institut des Sciences de la Vigne et du Vin, Écophysiologie et Génomique Fonctionnelle de la Vigne, UMR 1287, 210 Chemin de Leysotte, F-33140 Villenave-d’Ornon, France
(2) Biodiversité Gènes et Communautés, Institut National de la Recherche Agronomique (INRA), Université Bordeaux, 33610 Cestas, France

Contact the author

Keywords

 Embolism, drought, xylem cavitation, vessels, grapevine

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

Interpreting wine aroma: from aroma volatiles to the aromatic perception

Wine contains so many odorants that all its olfaction-related perceptions are, inevitably, the result of the interaction between many odorants.

The wine microbial ecosystem: Molecular interactions between yeast species and evidence for higher order interactions

Fermenting grape juice represents one of the oldest continuously maintained anthropogenic microbial environments and supports a well-mapped microbial ecosystem. Several yeast and bacterial species dominate this ecosystem, and some of these species are part of the globally most studied and best understood individual organisms. Detailed physiological, cellular and molecular data have been generated on these individual species and have helped elucidate complex evolutionary processes such as the domestication of wine yeast strains of the species Saccharomyces cerevisiae. These data support the notion that the wine making environment represents an ecological niche of significant evolutionary relevance. Taken together, the data suggest that the wine fermentation ecosystem is an excellent model to study fundamental questions about the working of microbial ecosystems and on the impact of biotic selection pressures on microbial ecosystem functioning. Indeed, and although well mapped, the rules and molecular mechanisms that govern the interactions between microbial species within this, and other, ecosystems remain underexplored. Here we present data derived from several converging approaches, including microbiome data of spontaneous fermentations, the population dynamics of constructed consortia, the application of biotic selection pressures in directed laboratory evolution, and the physiological and molecular analysis of pairwise and higher order interactions between yeast species. The data reveal the importance of cell wall-related elements in interspecies interactions and in evolutionary adaptation and suggest that predictive modelling and biotechnological control of the wine ecosystem during fermentation are promising strategies for wine making in future.

Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Varietal Riesling aroma relies strongly on the formation and liberation of bound aroma compounds. Floral monoterpenes, green C6-alcohols, fruity C13-norisoprenoids and spicy volatile phenols are predominantly bound to disaccharides, which are produced and stored in the grape berry during berry maturation. Grape processing aims to extract maximum amount of the precursors from the berry skin to increase the potential for a strong varietal aroma in the wine. Subsequent yeast selection plays an important part in this process.

Preliminary characterisation of mannoproteins from different wine yeast strains and impact on wine properties

Mannoproteins (MPs) are released from the yeast cell wall during alcoholic fermentation and aging on the lees, and influence aspects of wine quality such as haze formation and colour stability. Yet, as this is a slow process with microbiological and sensory risks, the exogenous addition of extracted MPs poses an efficient alternative. While Saccharomyces cerevisiae has long been studied as a prominent source for MPs extraction, their structure and composition greatly differ between yeast species. This may influence their behaviour in the wine matrix and subsequent impact on wine properties. However, although wine yeast species other than S. cerevisiae possibly present an untapped source of MPs, they are still ill-characterised in terms of chemical composition and influence on wine.