Macrowine 2021
IVES 9 IVES Conference Series 9 Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Abstract

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition. The study was carried out in a vineyard of A.O.C. Rioja, planted in 1999 with cv. Tempranillo (Vitis vinifera L.) grafted on 110-Richter rootstock (2,849 vines ha-1). Vines were trained on a double Cordon Royat. The treatments were: tillage (T), cover crop of barley (B) (Hordeum vulgare L.), and cover crop of clover (C) (Trifolium resupinatum L.). Each treatment consisted of three repetitions. Soil NO3–N was monitored at 0-15 and 15-45 cm soil depth at budbreak, bloom, fruit set, veraison and postharvest during four years (2009, 2010, 2011 and 2012). Soil NO3–N was extracted with 2 M KCl and determined by colorimetry. Grapevine N content was analyzed in leaf tissues (blade and petiole) sampled at bloom and veraison. Nitrogen content in leaves was determined by dry and instantaneous combustion. In each repetition, 15-20 grapevines were harvested. Wines were elaborated following the traditional method used in A.O.C. Rioja for red wines. The biogenic amines content in wines (histamine, methylamine, ethylamine, tyramine, phenylethylamine, putrescine, isoamylamine and cadaverine) was determined by HPLC. The results showed that the barley cover crop reduced soil NO3–N availability and clover cover crop increased it. Leaf tissues N content, at bloom of third year decreased with the barley treatment in both blade and petiole. In 2012, N content, in both leaf tissues at bloom, was greater with the clover treatment than with both the tillage and the barley treatments. Content of N in leaf tissues indicated that changes in the soil NO3–N affected levels of N in vines. In the fourth season, total content of biogenic amines in wine decreased in the barley treatment respect to both tillage and clover treatments. Correlations were observed between methylamine and the petiole N content at bloom, histamine and ethylamine respect to both leaf tissues at veraison and putrescine with blade at veraison. Finally, total biogenic amines was positively correlated with both leaf tissues at bloom and at veraison. In conclusion, the concentration of biogenic amines in wines can be affected by the N nutritional status of the grapevines, provoked by changes in the soil NO3–N availability as a result of the implanted cover crops effect.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Eva Peréz-Álvarez, Fernando Peregrina, Maria Cabrita

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique.

Reaction Mechanisms of Copper and Iron with Hydrogen Sulfide and Thiols in Model Wine

Fermentation derived sulfidic off-odors due to hydrogen sulfide (H2S) and low molecular weight thiols are commonly encountered in wine production and removed by Cu(II) fining. However, the mechanism underlying Cu(II) fining remains poorly understood, and generally results in increased Cu concentration that lead to deleterious reactions in finished wine. The present study describes a mechanistic investigation of the iron and copper mediated reaction of H2S, cysteine, 3-sulfanylhexan-1-ol, and 6-sulfanylhexan-1-ol with oxygen. The concentrations of H2S, thiols, oxygen, and acetaldehyde were monitored over time. It was found that Cu(II) was rapidly reduced by both H2S and thiols to Cu(I).

Colour assessment of port wines using colorimetric and spectrophotometric methods

Colour is an important quality parameter in wines and is the result of a complex mixture of pigments
(including anthocyanins and their derivatives, quinones, xanthyllium compounds, etc.). Red wine colour changes over time as pigments react between themselves and with other wine macromolecules
(particularly polyphenols). During wine tasting, colour is normally assessed on the outer rim of the wine profile in a tilted glass, since most wines are too opaque to be analysed in the middle of the glass. Therefore, depending on the depth of observation considered, the perception of wine colour can be different.