Macrowine 2021
IVES 9 IVES Conference Series 9 Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Abstract

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition. The study was carried out in a vineyard of A.O.C. Rioja, planted in 1999 with cv. Tempranillo (Vitis vinifera L.) grafted on 110-Richter rootstock (2,849 vines ha-1). Vines were trained on a double Cordon Royat. The treatments were: tillage (T), cover crop of barley (B) (Hordeum vulgare L.), and cover crop of clover (C) (Trifolium resupinatum L.). Each treatment consisted of three repetitions. Soil NO3–N was monitored at 0-15 and 15-45 cm soil depth at budbreak, bloom, fruit set, veraison and postharvest during four years (2009, 2010, 2011 and 2012). Soil NO3–N was extracted with 2 M KCl and determined by colorimetry. Grapevine N content was analyzed in leaf tissues (blade and petiole) sampled at bloom and veraison. Nitrogen content in leaves was determined by dry and instantaneous combustion. In each repetition, 15-20 grapevines were harvested. Wines were elaborated following the traditional method used in A.O.C. Rioja for red wines. The biogenic amines content in wines (histamine, methylamine, ethylamine, tyramine, phenylethylamine, putrescine, isoamylamine and cadaverine) was determined by HPLC. The results showed that the barley cover crop reduced soil NO3–N availability and clover cover crop increased it. Leaf tissues N content, at bloom of third year decreased with the barley treatment in both blade and petiole. In 2012, N content, in both leaf tissues at bloom, was greater with the clover treatment than with both the tillage and the barley treatments. Content of N in leaf tissues indicated that changes in the soil NO3–N affected levels of N in vines. In the fourth season, total content of biogenic amines in wine decreased in the barley treatment respect to both tillage and clover treatments. Correlations were observed between methylamine and the petiole N content at bloom, histamine and ethylamine respect to both leaf tissues at veraison and putrescine with blade at veraison. Finally, total biogenic amines was positively correlated with both leaf tissues at bloom and at veraison. In conclusion, the concentration of biogenic amines in wines can be affected by the N nutritional status of the grapevines, provoked by changes in the soil NO3–N availability as a result of the implanted cover crops effect.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Eva Peréz-Álvarez, Fernando Peregrina, Maria Cabrita

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Grape byproducts as source of resveratrol oligomers for the development of antifungal extracts

Grape canes are a non-recycled byproduct of wine industry (1-5 tons per hectare per year) containing valuable phytochemicals of medicine and agronomical interest. Resveratrol and wine polyphenols are known to exert a plethora of health-promoting effects including antioxidant capacity, cardioprotection, anticancer activity, anti-inflammatory effects, and estrogenic/antiestrogenic properties (Guerrero et al. 2009). Additionally, resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance (Chang et al. 2011). Our project aims to develop polyphenol-rich grape cane extracts to fight phytopathogenic or clinically relevant fungi. We initiate the project with the development of analytical methods to analyze resveratrol mono- and oligomers (dimers, trimers and tetramers) from grape canes and we evaluate their potential activity against clinically relevant opportunistic fungal pathogens (Houillé et al. 2014).

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Ageing of Sauvignon Blanc white wines with Specific Inactivated Dry Yeasts: Effect on physical and chemical characteristics

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Effects of bottle closure type on sensory characteristics of Chasselas wines

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.