Macrowine 2021
IVES 9 IVES Conference Series 9 Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Abstract

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition. The study was carried out in a vineyard of A.O.C. Rioja, planted in 1999 with cv. Tempranillo (Vitis vinifera L.) grafted on 110-Richter rootstock (2,849 vines ha-1). Vines were trained on a double Cordon Royat. The treatments were: tillage (T), cover crop of barley (B) (Hordeum vulgare L.), and cover crop of clover (C) (Trifolium resupinatum L.). Each treatment consisted of three repetitions. Soil NO3–N was monitored at 0-15 and 15-45 cm soil depth at budbreak, bloom, fruit set, veraison and postharvest during four years (2009, 2010, 2011 and 2012). Soil NO3–N was extracted with 2 M KCl and determined by colorimetry. Grapevine N content was analyzed in leaf tissues (blade and petiole) sampled at bloom and veraison. Nitrogen content in leaves was determined by dry and instantaneous combustion. In each repetition, 15-20 grapevines were harvested. Wines were elaborated following the traditional method used in A.O.C. Rioja for red wines. The biogenic amines content in wines (histamine, methylamine, ethylamine, tyramine, phenylethylamine, putrescine, isoamylamine and cadaverine) was determined by HPLC. The results showed that the barley cover crop reduced soil NO3–N availability and clover cover crop increased it. Leaf tissues N content, at bloom of third year decreased with the barley treatment in both blade and petiole. In 2012, N content, in both leaf tissues at bloom, was greater with the clover treatment than with both the tillage and the barley treatments. Content of N in leaf tissues indicated that changes in the soil NO3–N affected levels of N in vines. In the fourth season, total content of biogenic amines in wine decreased in the barley treatment respect to both tillage and clover treatments. Correlations were observed between methylamine and the petiole N content at bloom, histamine and ethylamine respect to both leaf tissues at veraison and putrescine with blade at veraison. Finally, total biogenic amines was positively correlated with both leaf tissues at bloom and at veraison. In conclusion, the concentration of biogenic amines in wines can be affected by the N nutritional status of the grapevines, provoked by changes in the soil NO3–N availability as a result of the implanted cover crops effect.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Eva Peréz-Álvarez, Fernando Peregrina, Maria Cabrita

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Innovations in the use of bentonite in enology: interactions with grape and wine proteins, colloids, polyphenols and aroma compounds.

The use of bentonite in oenology rounds around the limpidity and the stability that determine consumer acceptability. As a matter of fact, the haze formation in wine reduces its commercial value and makes it unacceptable for sale. Stabilization treatments are, therefore, essential to ensure a long-time limpidity and to forecast the formation of deposits in the bottle. Bentonite that is normally used in oenology for clarifying-fining purpose, shows a natural clay-based mineral structure allowing it to swell and to jelly in water and hence in must and wine.

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation.

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze.

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.