Macrowine 2021
IVES 9 IVES Conference Series 9 Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

Abstract

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition. The study was carried out in a vineyard of A.O.C. Rioja, planted in 1999 with cv. Tempranillo (Vitis vinifera L.) grafted on 110-Richter rootstock (2,849 vines ha-1). Vines were trained on a double Cordon Royat. The treatments were: tillage (T), cover crop of barley (B) (Hordeum vulgare L.), and cover crop of clover (C) (Trifolium resupinatum L.). Each treatment consisted of three repetitions. Soil NO3–N was monitored at 0-15 and 15-45 cm soil depth at budbreak, bloom, fruit set, veraison and postharvest during four years (2009, 2010, 2011 and 2012). Soil NO3–N was extracted with 2 M KCl and determined by colorimetry. Grapevine N content was analyzed in leaf tissues (blade and petiole) sampled at bloom and veraison. Nitrogen content in leaves was determined by dry and instantaneous combustion. In each repetition, 15-20 grapevines were harvested. Wines were elaborated following the traditional method used in A.O.C. Rioja for red wines. The biogenic amines content in wines (histamine, methylamine, ethylamine, tyramine, phenylethylamine, putrescine, isoamylamine and cadaverine) was determined by HPLC. The results showed that the barley cover crop reduced soil NO3–N availability and clover cover crop increased it. Leaf tissues N content, at bloom of third year decreased with the barley treatment in both blade and petiole. In 2012, N content, in both leaf tissues at bloom, was greater with the clover treatment than with both the tillage and the barley treatments. Content of N in leaf tissues indicated that changes in the soil NO3–N affected levels of N in vines. In the fourth season, total content of biogenic amines in wine decreased in the barley treatment respect to both tillage and clover treatments. Correlations were observed between methylamine and the petiole N content at bloom, histamine and ethylamine respect to both leaf tissues at veraison and putrescine with blade at veraison. Finally, total biogenic amines was positively correlated with both leaf tissues at bloom and at veraison. In conclusion, the concentration of biogenic amines in wines can be affected by the N nutritional status of the grapevines, provoked by changes in the soil NO3–N availability as a result of the implanted cover crops effect.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Eva Peréz-Álvarez, Fernando Peregrina, Maria Cabrita

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Modulating role of SO2 in white wine protein haze formation

Despite the extensive research performed during the last decades, the multifactorial mechanism responsible for the white wine protein haze formation is not fully characterized. Herein, a new model is proposed, which is based on the experimental identification of sulfur dioxide as a major modulating factor inducing wine protein haze upon heating. As opposed to other reducing agents, such as 2-mercaptoethanol, dithiothreitol and tris(2-carboxyethyl)phosphine hydrochloride (TCEP), the addition of SO2 to must/wine upon heating cleaves intraprotein disulfide bonds, hinders thiol-disulfide exchange during protein interactions and can lead to the formation of novel inter/intraprotein disulfide bonds. Those are eventually responsible for wine protein aggregation which follows a nucleation-growth kinetic model as shown by dynamic light scattering [1].

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues.

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.