Macrowine 2021
IVES 9 IVES Conference Series 9 The commercial yeast strain as a significant source of variance for tyrosol and hydroxytyrosol in white wine

The commercial yeast strain as a significant source of variance for tyrosol and hydroxytyrosol in white wine

Abstract

Tyrosol (TYR) and hydroxytyrosol (HYT) are bioactive phenols present in olive oil and wine, basic elements of the Mediterranean diet. TYR is reported in the literature for its interesting antioxidant, cardioprotective and anti-inflammatory properties. In wine, its concentration can reach values as high as about 40 mg/L [Pour Nikfardjam et al. 2007] but, more frequently, this phenol – derived from yeast metabolism of tyrosine during fermentation – is present at lower levels, generally higher in red wines compared to whites. HYT was measured for the first time by Di Tommaso et al. [1998] in Italian wines – with maximum values of 4.20 mg/L and 1.92 mg/L for red and white wines, respectively – while definitely lower concentrations have been found later in Greek samples. Concentrations of about 2-3 mg/L have been reported by Minuti et al. [2006] for red wines while Romboli et al. [2015] observed definitely higher concentrations – up to 25 mg/L – in case of slow fermentations of Sangiovese wines processed in lab-scale. Oddly, in spite of the non-negligible concentration of these compounds in wine, few data are available regarding the concentration variability of TYR and HYT due to not genetically engineered Saccharomyces cerevisiae strains available on the market and used in winery conditions. To investigate this variability, 7 yeast strains (Zymaflore VL1; Fermol Arome Plus; AWRI 796; La Claire EM2; Anchor VIN13; Zymaflore VL3; Mycoferm CRU 31) were used (15 g/hL) to ferment – on semi-industrial scale, at 18-21°C – five Pinot gris juices achieved from different vineyards. They were adequately provided with natural assimilable nitrogen (163-214 mg/L), and had been well settled (36 h, 10°C, < 100 NTU) and supplemented with 20-30 mg/L SO2. After alcoholic fermentation, wines were sulphited (80 mg/L) and maintained sur lies under argon blanketing (4°C x 90 days), with batonnage 1 time a week on average. In the transition from juice to wine, the mean concentrations of TYR and HYT increased about 60 and 20 times. In wine, TYR ranged between 4.20 and 15.51 mg/L, and HYT between 0.33 and 3.45 mg/L confirming the values in the literature. Statistically significant differences have been observed between yeast strains, both for TYR and HYT, and maximum variability between strain mean concentrations was about 35%, corresponding to a range of about 2.2 mg/L TYR and 0.55 mg/L HYT. In any case, the variability linked to the origin of the juice was higher than that linked to the Saccharomyces cerevisiae strain. Pour Nikfardjam et al. 2007]. Mitteilungen Klosterneuburg 57(3), 146-152 Di Tommaso et al. (1998). J. High Res. Chromatography 21(10), 549-553 Minuti et al. (2006). J. Chromatography A, 1114, 263-268 Romboli et al. (2015). W. J. Microbiol. Biotech. 31(7), 1137-1145.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Tomàs Villegas*, Chiara Barnaba, Giorgio Nicolini, Luca Debiasi, Roberto Larcher, Tiziana Nardin

*fondazione E.Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of smoke exposure on the chemical composition of grapes

Vineyard exposure to smoke can lead to grapes and wine which exhibit objectionable smoky and ashy aromas and flavours, more commonly known as ‘smoke taint’ [1, 2]. In the last decade, significant bushfires have occurred around the world, including near wine regions in Australia, Canada, South Africa and the USA, as a consequence of the warmer, drier conditions associated with climate change. Considerable research has subsequently been undertaken to determine the chemical, sensory and physiological consequences of grapevine exposure to smoke. The sensory attributes associated with smoke-tainted wine have been linked to the presence of several smoke-derived volatile phenols, such as guaiacols, syringols and cresols [2].

Testing the effectiveness of Cell-Wall material from grape pomace as fining agent for red wines

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.