Macrowine 2021
IVES 9 IVES Conference Series 9 The commercial yeast strain as a significant source of variance for tyrosol and hydroxytyrosol in white wine

The commercial yeast strain as a significant source of variance for tyrosol and hydroxytyrosol in white wine

Abstract

Tyrosol (TYR) and hydroxytyrosol (HYT) are bioactive phenols present in olive oil and wine, basic elements of the Mediterranean diet. TYR is reported in the literature for its interesting antioxidant, cardioprotective and anti-inflammatory properties. In wine, its concentration can reach values as high as about 40 mg/L [Pour Nikfardjam et al. 2007] but, more frequently, this phenol – derived from yeast metabolism of tyrosine during fermentation – is present at lower levels, generally higher in red wines compared to whites. HYT was measured for the first time by Di Tommaso et al. [1998] in Italian wines – with maximum values of 4.20 mg/L and 1.92 mg/L for red and white wines, respectively – while definitely lower concentrations have been found later in Greek samples. Concentrations of about 2-3 mg/L have been reported by Minuti et al. [2006] for red wines while Romboli et al. [2015] observed definitely higher concentrations – up to 25 mg/L – in case of slow fermentations of Sangiovese wines processed in lab-scale. Oddly, in spite of the non-negligible concentration of these compounds in wine, few data are available regarding the concentration variability of TYR and HYT due to not genetically engineered Saccharomyces cerevisiae strains available on the market and used in winery conditions. To investigate this variability, 7 yeast strains (Zymaflore VL1; Fermol Arome Plus; AWRI 796; La Claire EM2; Anchor VIN13; Zymaflore VL3; Mycoferm CRU 31) were used (15 g/hL) to ferment – on semi-industrial scale, at 18-21°C – five Pinot gris juices achieved from different vineyards. They were adequately provided with natural assimilable nitrogen (163-214 mg/L), and had been well settled (36 h, 10°C, < 100 NTU) and supplemented with 20-30 mg/L SO2. After alcoholic fermentation, wines were sulphited (80 mg/L) and maintained sur lies under argon blanketing (4°C x 90 days), with batonnage 1 time a week on average. In the transition from juice to wine, the mean concentrations of TYR and HYT increased about 60 and 20 times. In wine, TYR ranged between 4.20 and 15.51 mg/L, and HYT between 0.33 and 3.45 mg/L confirming the values in the literature. Statistically significant differences have been observed between yeast strains, both for TYR and HYT, and maximum variability between strain mean concentrations was about 35%, corresponding to a range of about 2.2 mg/L TYR and 0.55 mg/L HYT. In any case, the variability linked to the origin of the juice was higher than that linked to the Saccharomyces cerevisiae strain. Pour Nikfardjam et al. 2007]. Mitteilungen Klosterneuburg 57(3), 146-152 Di Tommaso et al. (1998). J. High Res. Chromatography 21(10), 549-553 Minuti et al. (2006). J. Chromatography A, 1114, 263-268 Romboli et al. (2015). W. J. Microbiol. Biotech. 31(7), 1137-1145.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Tomàs Villegas*, Chiara Barnaba, Giorgio Nicolini, Luca Debiasi, Roberto Larcher, Tiziana Nardin

*fondazione E.Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Supramolecular approaches to the study of the astringency elicited by wine phenolic compounds

The objective of this study is to review the scientific evidences and to advance into the knowledge of the molecular mechanisms of astringency. Astringency has been described as the drying, roughing and puckering sensation perceived when some food and beverages are tasted (1). The main, but possibly not the only, mechanism for the astringency is the precipitation of salivary proteins (2,3). Between phenolic compounds found in red wines, flavan-3-ols are the group usually related to the development of this sensation. Other compounds, phenolic or not, like anthocyanins, polysaccharides and mannoproteins could act modifying or modulating astringency perception by hindering the interaction between flavanols and salivary proteins either because of their interaction with the flavanols or because of their interaction with the salivary proteins.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

Effects of a new vacuum evaporation method on chemical and sensory properties of must and wine

A new process for vacuum evaporation was developed where evaporation takes place near the inner surface of a vortex produced by a rotor submerged in the liquid. Contrary to the state of the art the Vortex rotor process does not need a vacuum vessel but the rotating liquid creates a geometrically stable low pressure void surrounded by a vortex stabilized by the equilibrium between centrifugal forces and the pressure difference. First tests with water and sugar solutions at concentrations similar to grape must were conducted to verify the theoretical predictions, test the performance under different conditions and study the effect of various process parameters (Rösti et al 2015).

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

Simultaneous monitoring of dissolved CO2 and collar from Rosé sparkling wine glasses: the impact of yeast macromolecules

Champagne or sparkling wines elaborated through the same traditional method, which consists in two major yeast-fermented steps, typically hold about 10 to 12 g/L of dissolved CO2 after the second fermentation in a closed bottle. Hundreds of molecules and macromolecules originating from grape and yeast cohabit with dissolved CO2; they are essential compounds contributing to many organoleptic characteristics (effervescence, foam, aroma, taste, colour…). Indeed, the second alcoholic fermentation and the maturation on lees (which may last from 12 months up to several years) both induce various quantitative and qualitative changes in the wine through the action of yeast, as listed hereafter: development of aromas during aging on lees, release of nitrogen compounds during autolysis and release of macromolecules (polysaccharides, lipids, nucleic acids) in wine.