Macrowine 2021
IVES 9 IVES Conference Series 9 Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Abstract

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol). The initial phytosterol concentration determined the amount of consumed nitrogen and therefore the population reached in stationary phase and the maximal fermentation rate. An early loss of viability was observed when lipid concentrations were very low. For example, for an initial phytosterol concentration of 1 mg/L, the viability continuously decreased during the stationary phase and its final value was only 50%. In some fermentations, 10 mg/L oxygen were added at the end of the growth phase, to combine the effects of phytosterols from the musts and the de novo synthesis of ergosterol and unsaturated fatty acids. Oxygen additions highly improved the fermentation kinetics of media with low phytosterol contents. For example, in the medium containing 2 mg/L of phytosterol, the maximum fermentation rate was increased by 45 % and the fermentation time was 70 hours shorter. In the case of media containing 3, 5 and 8 mg/L of phytosterols, the assimilable nitrogen was completely exhausted and the fermentation kinetics as well as the final populations and viabilities (higher than 90%) were identical for the 3 conditions. Impacts of lipid content and oxygen addition on acetate and glycerol synthesis were also quantified. Acetate production was lower for high phytosterol concentrations whereas, in extreme phytosterol deficient musts, oxygenation resulted in a significant increase of both acetate and glycerol synthesis. Similar results were obtained with natural musts containing different amounts of solids. Consequently, this study points out the importance of controlling the nitrogen – lipid balance, especially in nitrogen-rich musts like in Champagne, and also the interest of combining a sufficient initial turbidity with an optimized oxygenation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Thomas Ochando*, Jean-Marie Sablayrolles, Jean-Roch Mouret, Vincent Farines

*INRA (UMR SPO)

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of industrial-scale serial filtration on macromolecules in red wines

Filtration is a critical step in ensuring the clarity and microbial stability of wine prior to bottling. However the process of filtering potentially reduces red wine quality by removing some of the macromolecules that contribute to the texture of the wine. Commercial red wines, Cabernet Sauvignon (CAS) and Shiraz (SHZ), of two vintages and two grades (premium grade wines from the older vintage: CAS13 and SHZ13; and standard grade wines from a younger vintage: CAS14 and SHZ14) were filtered through industrial-scale commercial filtration units prior to bottling. Samples were taken before and after cross-flow filtration, lenticular filters, 0.65 µm and 0.45 µm pore size nylon membrane filters. The concentration and composition of macromolecules, including tannins and polysaccharides, were measured in all samples as well as particle size distribution and wine colour.

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha.

Impact of glutathione and elemental sulphur juice addition on the volatile thiol production in South African Sauvignon blanc wine

Three compounds, 3-mercaptohexanol (3MH), 3-mercaptohexyl-acetate (3MHA) and 4-mercapto-4-methylpentan-2-one (4MMP), also known as varietal thiols, have been identified to contribute positively to wine aroma and are responsible for the distinct gooseberry, grapefruit, guava and box tree character found in Sauvignon blanc wines. Certain volatile thiol compounds though, can cause off-aromas of onion, garlic, rubber and rotten egg, this group of molecules is known as reductive sulphur compounds (RSC). This study looks into how the addition of sulphur-compounds to Sauvignon blanc juice contributes to the varietal thiol (3MH and 3MHA) concentration and reductive sulphur compound concentration in South African Sauvignon blanc wine.

Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Wine industry is looking forward for innovative, safe and eco-friendly antimicrobial products allowing the reduction of chemical treatments in the grape defense and the winemaking process that can affect negatively the quality of the product. Ozone has been tested in food industry giving good results in preventing fungi and bacteria growth on a wide spectrum of vegetables and fruits, due to its oxidant activity and ability to attack numerous cellular constituents. Ozone leaves no chemical residues on the food surface, decomposing itself rapidly in oxygen. Gaseous ozone has been already tested for table grapes storage and on wine grapes during withering.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.