Macrowine 2021
IVES 9 IVES Conference Series 9 Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Abstract

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol). The initial phytosterol concentration determined the amount of consumed nitrogen and therefore the population reached in stationary phase and the maximal fermentation rate. An early loss of viability was observed when lipid concentrations were very low. For example, for an initial phytosterol concentration of 1 mg/L, the viability continuously decreased during the stationary phase and its final value was only 50%. In some fermentations, 10 mg/L oxygen were added at the end of the growth phase, to combine the effects of phytosterols from the musts and the de novo synthesis of ergosterol and unsaturated fatty acids. Oxygen additions highly improved the fermentation kinetics of media with low phytosterol contents. For example, in the medium containing 2 mg/L of phytosterol, the maximum fermentation rate was increased by 45 % and the fermentation time was 70 hours shorter. In the case of media containing 3, 5 and 8 mg/L of phytosterols, the assimilable nitrogen was completely exhausted and the fermentation kinetics as well as the final populations and viabilities (higher than 90%) were identical for the 3 conditions. Impacts of lipid content and oxygen addition on acetate and glycerol synthesis were also quantified. Acetate production was lower for high phytosterol concentrations whereas, in extreme phytosterol deficient musts, oxygenation resulted in a significant increase of both acetate and glycerol synthesis. Similar results were obtained with natural musts containing different amounts of solids. Consequently, this study points out the importance of controlling the nitrogen – lipid balance, especially in nitrogen-rich musts like in Champagne, and also the interest of combining a sufficient initial turbidity with an optimized oxygenation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Thomas Ochando*, Jean-Marie Sablayrolles, Jean-Roch Mouret, Vincent Farines

*INRA (UMR SPO)

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure.

Prevention of wine oxidation during barrel aging: an innovative method to measure antioxidant

Wine oxidation is a problem that affects the freshness, the aromatic profile, the colour and also the mouthfeel of the wine. It mainly concerns white wines. Oxygen interactions with wine compounds lead to the phenomena cited above that are responsible for the depreciation of these wines. Barrel aging is a crucial step in the wine process because it allows many modifications as wine enrichment, colour stabilization, clarification and also a slow oxygenation of the wine. Effects of the oak barrel have to be known to prevent oxidation of the wine. We have been interested in the main antioxidant compounds released by oak barrels to the wine and we have developed an innovative method to reach directly these antioxidant compounds at the oak stave surface.

Using elicitors in different grape varieties. Effect over their phenolic composition

Phenolic compounds are very important in crop plants and have been the subject of a large number of studies. Three main reasons can be cited for optimizing the level of phenolic compounds in crop plants: their physiological role in plants, their technological significance for food processing, and their nutritional characteristics1 Indeed, an enormous diversity of phenolic antioxidants is found in fruits and vegetables, and their presence and roles can be affected or modified by several pre- and postharvest cultural practices and/or food processing technologies (Ruiz-García et al. 2012, Goldman et al. 1999, Tudela et al. 2002). In winegrapes, the technological importance of phenolic compounds, mainly flavonoids, is well-known.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.