Macrowine 2021
IVES 9 IVES Conference Series 9 Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Abstract

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol). The initial phytosterol concentration determined the amount of consumed nitrogen and therefore the population reached in stationary phase and the maximal fermentation rate. An early loss of viability was observed when lipid concentrations were very low. For example, for an initial phytosterol concentration of 1 mg/L, the viability continuously decreased during the stationary phase and its final value was only 50%. In some fermentations, 10 mg/L oxygen were added at the end of the growth phase, to combine the effects of phytosterols from the musts and the de novo synthesis of ergosterol and unsaturated fatty acids. Oxygen additions highly improved the fermentation kinetics of media with low phytosterol contents. For example, in the medium containing 2 mg/L of phytosterol, the maximum fermentation rate was increased by 45 % and the fermentation time was 70 hours shorter. In the case of media containing 3, 5 and 8 mg/L of phytosterols, the assimilable nitrogen was completely exhausted and the fermentation kinetics as well as the final populations and viabilities (higher than 90%) were identical for the 3 conditions. Impacts of lipid content and oxygen addition on acetate and glycerol synthesis were also quantified. Acetate production was lower for high phytosterol concentrations whereas, in extreme phytosterol deficient musts, oxygenation resulted in a significant increase of both acetate and glycerol synthesis. Similar results were obtained with natural musts containing different amounts of solids. Consequently, this study points out the importance of controlling the nitrogen – lipid balance, especially in nitrogen-rich musts like in Champagne, and also the interest of combining a sufficient initial turbidity with an optimized oxygenation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Thomas Ochando*, Jean-Marie Sablayrolles, Jean-Roch Mouret, Vincent Farines

*INRA (UMR SPO)

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Reaction Mechanisms of Copper and Iron with Hydrogen Sulfide and Thiols in Model Wine

Fermentation derived sulfidic off-odors due to hydrogen sulfide (H2S) and low molecular weight thiols are commonly encountered in wine production and removed by Cu(II) fining. However, the mechanism underlying Cu(II) fining remains poorly understood, and generally results in increased Cu concentration that lead to deleterious reactions in finished wine. The present study describes a mechanistic investigation of the iron and copper mediated reaction of H2S, cysteine, 3-sulfanylhexan-1-ol, and 6-sulfanylhexan-1-ol with oxygen. The concentrations of H2S, thiols, oxygen, and acetaldehyde were monitored over time. It was found that Cu(II) was rapidly reduced by both H2S and thiols to Cu(I).

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

What about oxygen transfer during wine aging in barrels?

During wine aging, several complex phenomena of gas transfer take place in barrels due to the wine/oak contact. The efficiency of this gas transfer varies according to oak wood’s intrinsic physical properties. This research aims to better understand oxygen transfer phenomena through dry oak staves and especially through stave gaps, in order to reevaluate the importance of barrel-making on a barrel’s supply of oxygen. Experimentation was based on the development of an innovative permeameter of laboratory scale, for which the principal operating conditions concerning applied pressure, the choice of liquid phase/gas phase, and the grain type of oak are taken into account and investigated. With a specially developed tightening system, the existing pressure at stave gaps in a barrel could be reproduced on a laboratory scale in order to estimate its influence on oxygen transfer efficiency.