Macrowine 2021
IVES 9 IVES Conference Series 9 Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Abstract

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol). The initial phytosterol concentration determined the amount of consumed nitrogen and therefore the population reached in stationary phase and the maximal fermentation rate. An early loss of viability was observed when lipid concentrations were very low. For example, for an initial phytosterol concentration of 1 mg/L, the viability continuously decreased during the stationary phase and its final value was only 50%. In some fermentations, 10 mg/L oxygen were added at the end of the growth phase, to combine the effects of phytosterols from the musts and the de novo synthesis of ergosterol and unsaturated fatty acids. Oxygen additions highly improved the fermentation kinetics of media with low phytosterol contents. For example, in the medium containing 2 mg/L of phytosterol, the maximum fermentation rate was increased by 45 % and the fermentation time was 70 hours shorter. In the case of media containing 3, 5 and 8 mg/L of phytosterols, the assimilable nitrogen was completely exhausted and the fermentation kinetics as well as the final populations and viabilities (higher than 90%) were identical for the 3 conditions. Impacts of lipid content and oxygen addition on acetate and glycerol synthesis were also quantified. Acetate production was lower for high phytosterol concentrations whereas, in extreme phytosterol deficient musts, oxygenation resulted in a significant increase of both acetate and glycerol synthesis. Similar results were obtained with natural musts containing different amounts of solids. Consequently, this study points out the importance of controlling the nitrogen – lipid balance, especially in nitrogen-rich musts like in Champagne, and also the interest of combining a sufficient initial turbidity with an optimized oxygenation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Thomas Ochando*, Jean-Marie Sablayrolles, Jean-Roch Mouret, Vincent Farines

*INRA (UMR SPO)

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Flavanol glycosides in grapes and wines : the key missing molecular intermediates in condensed tannin biosynthesis ?

Polyphenols are present in a wide variety of plants and foods such as tea, cacao and grape1. An important sub-class of these compounds is the flavanols present in grapes and wines as monomers (e.g (+)-catechin or (-)-epicatechin), or polymers also called condensed tannins or proanthocyanidins. They have important antioxidant properties2 but their biosynthesis remains partly unknown. Some recent studies have focused on the role of glycosylated intermediates that are involved in the transport of the monomers and may serve as precursors in the polymerization mechanism3, 4. The global objective of this work is to identify flavanol glycosides in grapes or wines, describe their structure and determine their abundance during grape development and in wine.

Petrolomics-derived data interpretation to study acetaldehyde-epicatechin condensation reactions

During red wine ageing or conservation, color and taste change and astringency tends to reduce. These changes result from reactions of flavan-3-ols and/or anthocyanins among which condensation reactions with acetaldehyde are particularly important. The full characterization of these reactions has not been fully achieved because of difficulties in extracting and separating the newly formed compounds directly from wine. Model solutions mimicking food products constitute a simplified medium for their exploration, allowing the detection of the newly formed compounds, their isolation, and their structure elucidation.

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.