Macrowine 2021
IVES 9 IVES Conference Series 9 Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Abstract

Oak barrels form an integral part of wine production, especially that of high quality wines. However, due to its porosity, wood presents an ecological niche for microbial proliferation and is highly susceptible to microbial spoilage which could cause considerable economic losses. Brettanomyces bruxellensis, the most commonly encountered microorganism responsible for spoilage during barrel ageing, can remain in barrels after barrel sanitation to contaminate new batches of wine after refilling. Therefore, effective sanitation treatments are of utmost importance to prevent recurring wine spoilage. Since a moratorium on the use of sulphur dioxide, the most widely used biocide for barrel sanitation, has been issued by the European Commission, there is a drastic need to evaluate alternative sanitation methods. Literature on the effectiveness of barrel sanitation treatments is scattered and presents inconclusive results. Furthermore, all studies have used culture-dependent methods to detect Brettanomyces which has been reported to attain a viable but non-culturable state [1,2]. Therefore, the aim of this study was to evaluate several physical and chemical sanitation treatments in their efficiency to eliminate B. bruxellensis from oak wood. French oak wood was contaminated with different B. bruxellensis strains and subsequently subjected to several chemical and physical sanitation treatments. The methods included, among others, ozone gas, ozonated water, high pressure ultrasound, steam, peracetic acid, sodium percarbonate and microwave. After their extraction from the oak wood, Brettanomyces cells were subjected to a fluorescence-based live/dead staining and detected by means of flow cytometry. The treatments differed greatly in their ability to reduce B. bruxellensis cells with ozone gas showing promising results. Sodium percarbonate and steam treatments were also effective in reducing cells. This study presents the first evaluation of alternative barrel sanitation treatments by flow cytometry, a culture-independent method, and is also the first study to compare an array of barrel sanitation treatments under controlled conditions.

1. Agnolucci, M., Rea, F., Sbrana, C., Cristani, C., Fracassetti, D., Tirelli, A., Nuti, M., 2010. Sulphur dioxide affects culturability and volatile phenol production by Brettanomyces/ Dekkera bruxellensis. International Journal of Food Microbiology 143, 76-80. 2. Serpaggi, V., Remize, F., Recorbet, G., Gaudot-Dumas, E., Sequeira-Le Grand, A., Alexandre, H., 2012. Characterization of the “viable but nonculturable”(VBNC) state in the wine spoilage yeast Brettanomyces. Food Microbiology 30, 438-447.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Engela Kritzinger*, Maren Scharfenberger-Schm, Ulrich Fischer

*DLR Rheinpfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Field-grown Sauvignon Blanc berries react to increased exposure by controlling antioxidant homeostasis and displaying UV acclimation responses that are influenced by the level of ambient light

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).

Non-invasive headspace sorptive extraction for monitoring volatile compounds production by saccharomyces and non-saccharomyces strains throughout alcoholic fermentation

Wine is a solution containing abundant volatile compounds which contribute to their aroma. Many of them are produced by yeast as metabolism by-products. Different yeast strains produce different volatile profiles. The possibility of studying the evolution of volatile compounds during fermentation, using sampling methods that not alter the volume of fermentation media, is of great interest. In spite of this, non-invasive methods to monitoring the evolution of volatile profile during fermentation have been seldom used. The goals of this work were to use by first time the headspace sorptive extraction (HSSE) as non-invasive method to monitor the evolution of volatile profiles throughout alcoholic fermentation and to study the changes on volatile profiles produced by Saccharomyces cerevisiae and Lachancea thermotolerans during fermentation of a must with high sugar content.

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).