Macrowine 2021
IVES 9 IVES Conference Series 9 Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Abstract

Oak barrels form an integral part of wine production, especially that of high quality wines. However, due to its porosity, wood presents an ecological niche for microbial proliferation and is highly susceptible to microbial spoilage which could cause considerable economic losses. Brettanomyces bruxellensis, the most commonly encountered microorganism responsible for spoilage during barrel ageing, can remain in barrels after barrel sanitation to contaminate new batches of wine after refilling. Therefore, effective sanitation treatments are of utmost importance to prevent recurring wine spoilage. Since a moratorium on the use of sulphur dioxide, the most widely used biocide for barrel sanitation, has been issued by the European Commission, there is a drastic need to evaluate alternative sanitation methods. Literature on the effectiveness of barrel sanitation treatments is scattered and presents inconclusive results. Furthermore, all studies have used culture-dependent methods to detect Brettanomyces which has been reported to attain a viable but non-culturable state [1,2]. Therefore, the aim of this study was to evaluate several physical and chemical sanitation treatments in their efficiency to eliminate B. bruxellensis from oak wood. French oak wood was contaminated with different B. bruxellensis strains and subsequently subjected to several chemical and physical sanitation treatments. The methods included, among others, ozone gas, ozonated water, high pressure ultrasound, steam, peracetic acid, sodium percarbonate and microwave. After their extraction from the oak wood, Brettanomyces cells were subjected to a fluorescence-based live/dead staining and detected by means of flow cytometry. The treatments differed greatly in their ability to reduce B. bruxellensis cells with ozone gas showing promising results. Sodium percarbonate and steam treatments were also effective in reducing cells. This study presents the first evaluation of alternative barrel sanitation treatments by flow cytometry, a culture-independent method, and is also the first study to compare an array of barrel sanitation treatments under controlled conditions.

1. Agnolucci, M., Rea, F., Sbrana, C., Cristani, C., Fracassetti, D., Tirelli, A., Nuti, M., 2010. Sulphur dioxide affects culturability and volatile phenol production by Brettanomyces/ Dekkera bruxellensis. International Journal of Food Microbiology 143, 76-80. 2. Serpaggi, V., Remize, F., Recorbet, G., Gaudot-Dumas, E., Sequeira-Le Grand, A., Alexandre, H., 2012. Characterization of the “viable but nonculturable”(VBNC) state in the wine spoilage yeast Brettanomyces. Food Microbiology 30, 438-447.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Engela Kritzinger*, Maren Scharfenberger-Schm, Ulrich Fischer

*DLR Rheinpfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Defining the mechanisms and impact of winemaking treatments on tannin and polysaccharides in red wine: recent progress in creating diverse styles

Tannin and polysaccharide concentration and composition is important in defining the texture of red wines, but can vary due to factors such as cultivar, region, grape ripeness, viticultural practices and winemaking techniques. However, the concentration and composition of these macromolecules is dependent not only on grape tannin and polysaccharide concentration and composition, but also their extractability and, in the case of polysaccharides, their formation by yeast. Through studies into the influence of grape maturity, winemaking and sensory impacts of red grape polysaccharides, seed and skin tannins, recent research in our laboratory has shown that the processes involved in the extraction of these macromolecules from grapes and their retention in wine are very complex.

Evaluation of Polarized Projective Mapping as a possible tool for attributing South African Chenin blanc dry wine styles

Multiple Factor Analysis (MFA) According to the Chenin blanc Association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded (RRU), and Rich and Ripe Wooded (RRW), classically attributed with the help of sensory evaluation. One of the “rapid methods” has drawn our attention for the purpose of simplifying and making style attribution for large sample sets, evaluated during different sessions, more robust. Polarized Projective Mapping (PPM) is a hybrid of Projective Mapping (PM) and Polarised Sensory Positioning (PSP). It is a reference-based method in which poles
(references) are used for the evaluation of similarities and dissimilarities between samples.

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

Study of the colour and phenolic evolution of three different tannin/anthocyanin ratios over time in a model wine

Phenolic compounds are important quality indicators in red wine. A large number of polyphenols play an important role in wine development, contributing to the colour and the sensory perception of the wines. Anthocyanins are the pigments responsible for the colour in young red wines while tannins are the principal contributors to the bitterness and the astringency of the wines. Wine polyphenols are considered more complex molecules than grape phenolics, due to the enormous number of chemical reactions which take place during the entire winemaking process and storage, forming more stable compounds.

Improving the phenolic composition of cv tempranillo wines by blending grapes of different ripening state

The aim of this work was to reduce the alcohol content of Tempranillo wine. Tempranillo wines were produced by grapes harvested at different ripening dates (August 11 which was 21 oBrix and September 28 with 25 oBrix). At the second date, the Tempranillo wines were elaborated as follows: grapes were destemmed, crushed and collected into 50 L stainless-steel vats. Before preferementative maceration in cold, 50 % (M1) and 70 % (M2) of the must have been replaced by the same percentage of must from the first harvest. In addition, a control wine (C) was performed with only grapes from the second harvest.