Macrowine 2021
IVES 9 IVES Conference Series 9 Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Abstract

Oak barrels form an integral part of wine production, especially that of high quality wines. However, due to its porosity, wood presents an ecological niche for microbial proliferation and is highly susceptible to microbial spoilage which could cause considerable economic losses. Brettanomyces bruxellensis, the most commonly encountered microorganism responsible for spoilage during barrel ageing, can remain in barrels after barrel sanitation to contaminate new batches of wine after refilling. Therefore, effective sanitation treatments are of utmost importance to prevent recurring wine spoilage. Since a moratorium on the use of sulphur dioxide, the most widely used biocide for barrel sanitation, has been issued by the European Commission, there is a drastic need to evaluate alternative sanitation methods. Literature on the effectiveness of barrel sanitation treatments is scattered and presents inconclusive results. Furthermore, all studies have used culture-dependent methods to detect Brettanomyces which has been reported to attain a viable but non-culturable state [1,2]. Therefore, the aim of this study was to evaluate several physical and chemical sanitation treatments in their efficiency to eliminate B. bruxellensis from oak wood. French oak wood was contaminated with different B. bruxellensis strains and subsequently subjected to several chemical and physical sanitation treatments. The methods included, among others, ozone gas, ozonated water, high pressure ultrasound, steam, peracetic acid, sodium percarbonate and microwave. After their extraction from the oak wood, Brettanomyces cells were subjected to a fluorescence-based live/dead staining and detected by means of flow cytometry. The treatments differed greatly in their ability to reduce B. bruxellensis cells with ozone gas showing promising results. Sodium percarbonate and steam treatments were also effective in reducing cells. This study presents the first evaluation of alternative barrel sanitation treatments by flow cytometry, a culture-independent method, and is also the first study to compare an array of barrel sanitation treatments under controlled conditions.

1. Agnolucci, M., Rea, F., Sbrana, C., Cristani, C., Fracassetti, D., Tirelli, A., Nuti, M., 2010. Sulphur dioxide affects culturability and volatile phenol production by Brettanomyces/ Dekkera bruxellensis. International Journal of Food Microbiology 143, 76-80. 2. Serpaggi, V., Remize, F., Recorbet, G., Gaudot-Dumas, E., Sequeira-Le Grand, A., Alexandre, H., 2012. Characterization of the “viable but nonculturable”(VBNC) state in the wine spoilage yeast Brettanomyces. Food Microbiology 30, 438-447.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Engela Kritzinger*, Maren Scharfenberger-Schm, Ulrich Fischer

*DLR Rheinpfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.

Anthropogenic factors in modulations of fungal populations from grapes to wines and their repercussions on wine characteristics

The effects of anthropogenic activities on vineyard (different plant protections) and in winery
(pressing/clarification step, addition of sulfur dioxide) on fungal populations from grape to wine were studied. The studied anthropogenic activities modify the fungal diversity. Thus, lower biodiversity of grapes from organic modality was measured for the three vintages considered compared to biodiversity from ecophyto modality and conventional modality. The pressing / clarification steps strongly modify fungal populations and the influence of the winery flora is highlighted.

Ageing of sweet wines: oxygen evolution according to bung and barrel type

Barrel ageing is a crucial step in the wine process because it allows many changes to the wine as enrichment, colour stabilization, clarification and also a slow oxygenation. Effects of the oak barrel have to be known to prevent oxidation of the wine. The type of bung used during ageing is also a parameter to consider. Ageing sweet wines in barrel is a real challenge. These wines may need some oxygen at the beginning of ageing but they should be protected at the end of their maturation, to avoid oxidation.