Macrowine 2021
IVES 9 IVES Conference Series 9 Development and validation of a standardized oxidation assay for the accurate measurement of the ability of different wines to form “de novo” oxidation-related aldehydes

Development and validation of a standardized oxidation assay for the accurate measurement of the ability of different wines to form “de novo” oxidation-related aldehydes

Abstract

From the standpoint of wine aroma oxidation there are two effects observed: aroma degradation of oxygen sensitive compounds (polyfunctional mercaptans) and the appearance of new substances with high aromatic power (acetaldehyde, methional, phenylacetaldehyde, sotolon, alkenals, isobutanal and 2, 3-metylbutanals) (1-5). According to our experience, Strecker aldehydes are compounds with highest sensory relevance in the oxidative degradation of many wines (5-7). Based on previous research, it was observed that these compounds can be already present in freshly bottled wines, free from any sign of oxidation; forming stable, non-volatile and odorless complexes with sulfur dioxide (8). During storage in the bottle these compounds are released as the level of free SO2 decreases by oxidation causing a shift in the SO2-aldehyde adduct chemical equilibria. Moreover, wine aldehydes can be formed throught direct oxidation of their precursors (“de novo” formation), when the free SO2 level is under 5 mg/l (7-8). The main goal of this work is to study the intrinsic ability of the wines for the formation of “aldehydes de novo”. Hence, a method to consume oxygen at controlled doses, at 45 ° C, has been developed. This oxidation method allows to reach de novo formation of aldehydes on a fast way (2-7 days) depending on the wine. The validation of this method is carried out maintaining the same oxidation conditions at 25º C. In addition, the same wines have been submitted to consecutive air saturation cycles (9) for means of comparation. The proposed strategy comprises the study of eight red wines in duplicate, each wine underwent three increasing oxygen doses. The analysis carried out at the beginning at the and end of the oxidation were: aminoacids, metals, free and total SO2, total carbonyl compounds, acetaldehyde, color, IPT, Folin, as well as major and trace aroma compounds. The results show that this is a reproducible method of oxidation, which allows to reach de novo formation of aldehydes at all doses studied. Different profiles of oxygen consumption are obtained depending on the age and previous contact with oxygen, temperature had a strong effect on the formation of Strecker aldehydes with respect to the oxygen consumed.

1. Wildenradt et al., AJEV,1974, 25, 119 2. Escudero et al., JAFC, 2000, 48, 4268 3. Ferreira, A.C.S et al., JAFC, 2003, 51, 1377 4. Cutzach et al., JISVV, 1998, 32, 211 5. Culleré et al., JAFC, 2007, 55, 876 6. San Juan et al., JAFC, 2012, 60, 5045 7. Ferreira et al., JAFC, 2014,62, 10015 8. Bueno et al., JAFC., DOI 10.102117acs.jafc5b04634 9. Ferreira et al. ., JAFC., 2015, 63, 10928

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Almudena Marrufo-Curtido*, Ana Escudero, Ignacio Ontañon, Mónica Bueno, Vanesa Carrascon, Vicente Ferreira

*

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Phenolic profiles of minor red grape cultivars autochthonous from the Spanish region of La Mancha

The phenolic profiles of little known red grape cultivars, namely Garnacho, Moribel and Tinto Fragoso, which are autochthonous from the Spanish region of La Mancha (ca. 600,000 ha of vineyards) have been studied over the consecutive seasons of years 2013 and 2014. The study was separately performed over the skins, the pulp and the seeds, and comprised the following phenolic types: anthocyanins, flavonols, hydroxycinnamic acid derivatives (HCADs), total proanthocyanidins (PAs) and their structural features. The selected grape cultivars belong to the Vine Germplasm Bank created in this region in order to preserve the great diversity of genotypes grown in La Mancha.

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality.

Metabolomics comparison of non-Saccharomyces yeasts in Sauvignon blanc and Shiraz

Saccharomyces cerevisiae (SC) is the main driver of alcoholic fermentation however, in wine, non-Saccharomyces species can have a powerful effect on aroma and flavor formation. This study aimed to compare untargeted volatile compound profiles from SPME-GC×GC-TOF-MS of Sauvignon blanc and Shiraz wine inoculated with six different non-Saccharomyces yeasts followed by SC. Torulaspora delbrueckii (TD), Lachancea thermotolerans (LT), Pichia kluyveri (PK) and Metschnikowia pulcherrima (MP) were commercial starter strains, while Candida zemplinina (CZ) and Kazachstania aerobia (KA), were isolated from wine grape environments. Each fermentation produced a distinct chemical profile that was unique for both grape musts. The SC-monoculture and CZ-SC sequential fermentations were the most distinctly different in the Sauvignon blanc while the LT-SC sequential fermentations were the most different from the control in the Shiraz fermentations.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.