Macrowine 2021
IVES 9 IVES Conference Series 9 Development and validation of a standardized oxidation assay for the accurate measurement of the ability of different wines to form “de novo” oxidation-related aldehydes

Development and validation of a standardized oxidation assay for the accurate measurement of the ability of different wines to form “de novo” oxidation-related aldehydes

Abstract

From the standpoint of wine aroma oxidation there are two effects observed: aroma degradation of oxygen sensitive compounds (polyfunctional mercaptans) and the appearance of new substances with high aromatic power (acetaldehyde, methional, phenylacetaldehyde, sotolon, alkenals, isobutanal and 2, 3-metylbutanals) (1-5). According to our experience, Strecker aldehydes are compounds with highest sensory relevance in the oxidative degradation of many wines (5-7). Based on previous research, it was observed that these compounds can be already present in freshly bottled wines, free from any sign of oxidation; forming stable, non-volatile and odorless complexes with sulfur dioxide (8). During storage in the bottle these compounds are released as the level of free SO2 decreases by oxidation causing a shift in the SO2-aldehyde adduct chemical equilibria. Moreover, wine aldehydes can be formed throught direct oxidation of their precursors (“de novo” formation), when the free SO2 level is under 5 mg/l (7-8). The main goal of this work is to study the intrinsic ability of the wines for the formation of “aldehydes de novo”. Hence, a method to consume oxygen at controlled doses, at 45 ° C, has been developed. This oxidation method allows to reach de novo formation of aldehydes on a fast way (2-7 days) depending on the wine. The validation of this method is carried out maintaining the same oxidation conditions at 25º C. In addition, the same wines have been submitted to consecutive air saturation cycles (9) for means of comparation. The proposed strategy comprises the study of eight red wines in duplicate, each wine underwent three increasing oxygen doses. The analysis carried out at the beginning at the and end of the oxidation were: aminoacids, metals, free and total SO2, total carbonyl compounds, acetaldehyde, color, IPT, Folin, as well as major and trace aroma compounds. The results show that this is a reproducible method of oxidation, which allows to reach de novo formation of aldehydes at all doses studied. Different profiles of oxygen consumption are obtained depending on the age and previous contact with oxygen, temperature had a strong effect on the formation of Strecker aldehydes with respect to the oxygen consumed.

1. Wildenradt et al., AJEV,1974, 25, 119 2. Escudero et al., JAFC, 2000, 48, 4268 3. Ferreira, A.C.S et al., JAFC, 2003, 51, 1377 4. Cutzach et al., JISVV, 1998, 32, 211 5. Culleré et al., JAFC, 2007, 55, 876 6. San Juan et al., JAFC, 2012, 60, 5045 7. Ferreira et al., JAFC, 2014,62, 10015 8. Bueno et al., JAFC., DOI 10.102117acs.jafc5b04634 9. Ferreira et al. ., JAFC., 2015, 63, 10928

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Almudena Marrufo-Curtido*, Ana Escudero, Ignacio Ontañon, Mónica Bueno, Vanesa Carrascon, Vicente Ferreira

*

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Moscatel vine-shoot extracts as grapevine biostimulant to increase the varietal aroma of Airén wines

There is a growing interest in the exploitation of vine-shoots waste, since they are often left or burned. Sánchez-Gómez et al. [1] have shown that vines-shoots aqueous extracts have significant contents of bioactive compounds, among which several polyphenols and volatiles are highlighted. Recent studied had demonstrated that the chemical composition of vine-shoots is enhanced when vine-shoots are toasted
[2,3]. The application of vegetable products in the vineyards has led to significant changes towards a more “Sustainable Viticulture”. An innovative foliar application for Airén vine-shoot extracts have been carried out to the vineyard. It has been shown that they act as grape biostimulants, improving certain wine quality characteristics [4].

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation.

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method.

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).