Macrowine 2021
IVES 9 IVES Conference Series 9 Characterizing the effects of nitrogen on grapevines with different scion/rootstock combinations: agronomic, metabolomic and transcriptomic approaches

Characterizing the effects of nitrogen on grapevines with different scion/rootstock combinations: agronomic, metabolomic and transcriptomic approaches

Abstract

Most vineyards are grafted and include a variety (Vitis vinifera) grafted over a wild Vitis rootstock (hybrids of V. berlandieri, riparia and rupestris). Grape berry quality at harvest depends on a subtle balance between acidity and the concentrations of sugars, polyphenols and precursors of aroma compounds. The mechanisms controlling the balance of sugars/acids/polyphenols are influenced by the abiotic environment, in particular nitrogen supply, and interact with the genotypes of both the scion variety and the rootstock. Previous work suggests that some of the effects of water stress are in fact linked to a nitrogen deficiency driven indirectly by the reduction of water absorption. The root system (i.e rootstock) plays an important role in the uptake, reduction, transport and storage of nitrogen, and the water balance of the plant. In this context, we studied the mechanisms involved in the regulation of the synthesis of flavonoids in berries in response to nitrogen nutrition with different scion/rootstock combinations. Two varieties (Cabernet Sauvignon and Pinot Noir) were subjected to different nitrogen supplies in two experimental systems, in pots under semi-controlled conditions and in a vineyard. Agronomic analysis confirmed that high nitrogen supply increased the nitrogen content of different organs (leaf blades, petioles and berries) as well as leaf surface area and cane pruning weight. Metabolomic analyses of berry skins revealed an accumulation of secondary metabolites whose nature depended on the different rootstock/scion combinations studied. In addition, an increase in the synthesis of anthocyanins and flavonols was observed in the berry skins in response to the decrease in nitrogen nutrition. High nitrogen supply also increased the average degree of polymerization of tannins, while the contents of flavan-3-ols and procyanidins in the seeds and skins of the berries were not affected. Global transcriptome (using RNA sequencing) and targeted (qPCR) analyses showed changes in the abundance of transcripts of genes related to the metabolism of flavonoids in response to nitrogen status. Nitrogen supply also influenced the transcript amounts of positive (MYB) and negative (Lateral Boundary Organ Domain) transcription factors controlling of the biosynthesis of flavonoids.

Acknowledgements: This work was funded by the Conseil Interprofessionnel du Vin de Bordeaux, the KBBE Innovine project and COST Action FA 1106.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Serge Delrot*, Aude Habran, Cornelis van Leeuwen, Eric Gomes, Flavia Guzzo, Ghislaine Hilbert, Mauro Commisso, Pierre Helwi, Stefano Negri

*UMR1287-EGFV

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Determination of metallic elements in Chilean wines by atomic absorption spectroscopy and inductively coupled plasma–mass spectrometry

The chemical composition of wines depends on series of variables such as the type of grape, edaphoclimatic conditions, and viticulture and winemaking practices employed during production. Metallic elements play a significant role during winemaking (e.g. as catalysts of oxidation reactions) and have been previously employed for the classification of wines according to provenance. In this work, we focused on the analysis of metallic elements (K, Na, Ca, Zn, Cu, Fe, Mg, Mn, Ni, Cr, Al, Pb, Cd, Hg, Se, Co, Sn and As) in 145 Chilean wine samples (102 reds and 43 white wines), of seven grape varieties, and five of the major wine producing regions in Chile.

Microbial life in the grapevine: what can we expect from the leaf microbiome?

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents.

Application of high power ultrasounds during red wine vinification

Wine color is one of the main organoleptic characteristics influencing its quality. It is of especial interest in red vinifications due to the economic resources that wineries have to invest for the extraction of the phenolic compounds responsible of wine color, compounds that are mainly located inside the skin cell vacuoles. Moreover, these phenolic compounds not only influence color but also other organoleptic properties such as body, mouthfeel, astringency and flavour. The transference of phenolic compounds from grapes to must during vinification is closely related with the type of grapes and the winemaking technique.

A preliminary study of clonal selection in cv. Viura in relation to varietal aroma profile

Viura is a synonym for Macabeo and currently it is the most widely planted white grape variety in D.O.Ca. Rioja, with 3,569 ha, representing 84% of the white grape cultivated area. It is a generous-yielding grape, presenting low values of titratable acidity and with large and compact clusters which makes it susceptible to Botrytis cinerea. Thus, this variety not always satisfies the wine grower’s prospects. Nowadays, the available plant material is scarce, moreover, it was selected on the basis of other quality criteria, not currently requested.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.