terclim by ICS banner
IVES 9 IVES Conference Series 9 The role of NAC61 transcription factor in the regulation of berry ripening progression 

The role of NAC61 transcription factor in the regulation of berry ripening progression 


The undergoing global warming scenario is affecting grapevines phenology, including the timing of berry ripening and harvest date, negatively impacting production and quality. This work reports the crucial role of NAC61, a grapevine NAC transcription factor, in regulating metabolic processes occurring from the onset of ripening onwards. NAC61 high confidence targets mainly represent genes acting on stilbene biosynthesis and regulation, and in osmotic and oxidative/biotic stress-related responses. The direct regulation of the stilbene synthase regulator MYB14, the osmotic stress-related gene DHN1b, and the Botrytis cinerea susceptibility gene WRKY52, were all further validated. We demonstrate that NAC61 self-activates and is targeted by NAC60, another master regulator of grapevine organ maturation. Moreover, NAC61 physically interacts with NAC60 triggering the activation of common targets. In our studies, several NAC-NAC synergistic interactions were demonstrated, allowing us to suppose the existence of a NAC-dependent regulatory network orchestrating berry ripening and whose exploration is our current main purpose. As members of such regulatory network, we defined a core of 13 NACs highly correlated with NAC60, NAC61 and NAC33, the latter? being a repressor of vegetative organ growth during the vegetative-to-mature phase transition. By using DAP-Seq combined with transcriptomic data and functional assays we reconstructed a hierarchical intra-family regulatory network. We confirmed NAC60 and NAC33 as high hierarchy activators and we traced the downstream network genes been active in fruit ripening. This work is of high interest as identifying key regulators governing berry ripening progression provides important biomarkers affecting quality of grapes and wine.


Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster


Alessandra Amato1*, Chiara Foresti1, Luis Orduña2, Oscar Bellon1, Elodie Vandelle1, José Tomás Matus2,  Giovanni Battista Tornielli1, Sara Zenoni1

1 Department of Biotechnology, University of Verona, Verona, Italy
2 Institute for Integrative Systems Biology, Universitat de València-CSIC,46980 Paterna, Valencia, Spain

Contact the author*


Ripening, NAC transcription factors, Regulatory network, Functional analysis, DAP-Seq


IVES Conference Series | Open GPB | Open GPB 2024


Related articles…

Exploring grapevine water relations in the context of fruit growth at pre- and post-veraison

Climate change is increasing the frequency of water deficit in many grape-growing regions. Grapevine varieties differ in their stomatal behavior during water deficit, and their ability to regulate water potential under dry soil conditions is commonly differentiated using the concept of isohydricity. It remains unclear whether stomatal behavior, water potential regulation, and the resulting degree of isohydricity has a relationship with changes to fruit growth during water deficit. This study was conducted on four varieties (`Cabernet Franc`, `Semillon`, `Grenache`, and `Riesling`) subjected to both short-term, severe water deficit and long-term, moderate water deficit applied at both pre- and post-veraison.

Improved analysis of isomeric polyphenol dimers using the 4th dimension of trapped ion mobility spectrometry – mass spectrometry

Dehydrodicatechins have recently received attention as oxidation markers especially in grapes and wine. Their analysis mainly uses LC-MS/MS which is able to differentiate them from their natural isomers (dimeric procyanidins), based on specific fragments

Typicality related to terroir: from conceptual to perceptual representation: study of the links with enological practices

The conceptual image of a wine related to the terroir has consequences in technical terms. Among factors affecting the typicality, producers put forward the environmental factors of the terroir system

Influence of soil type on juice quality in a vineyard from DO Ca Rioja

Soil plays an important role in wine quality, especially its water holding capacity because it affects the balance between vigour and grape yield. The aim of this work was to study the influence of different soil types on the must quality in a vineyard at DO Ca Rioja

New disease-resistant grapevine varieties response to drought under a semi-arid climate

In many regions, climate change leads to an increase in air temperature combined with a reduction of rainfall, intensifying climatic demand and water deficits (WD) (Cardell et al. 2019), which in turn may negatively impact grapevine development, yield and grape composition (Santos et al. 2020). In addition, climate change may also increase disease pressure, leading to further yield and quality losses, besides increasing costs due to increased vineyard spraying (Santos et al. 2020) and reducing viticulture acceptability by consumers (Guichard et al. 2017). Adopting new resistant varieties appears as a promising long-term solution to better manage vine protection, but unfortunately little is known regarding their behavior in front of WD.