terclim by ICS banner
IVES 9 IVES Conference Series 9 Functional characterisation of genetic elements regulating bunch morphology in grapevine

Functional characterisation of genetic elements regulating bunch morphology in grapevine

Abstract

Vitis vinifera L., is considered one of the world’s most important cultivated fruit crops. In agriculture, bunch morphology is a grapevine-specific trait, which directly impacts fruit quality and health.
Bunch size, shape, and compactness are major aspects of bunch morphology, with the degree of compactness emerging as an important trait for grapevine genetic enhancement and vineyard management. The importance of this trait stems from its impact on disease susceptibility, berry ripening, and other grape quality properties. However, current knowledge of the genes controlling it remains limited.
This study aims to identify and characterise genetic elements regulating grapevine bunch formation, while also providing valuable understanding of molecular and cellular regulation of this important process. This will be done by carrying out three planned objectives, summarised as: identification of candidate genes, overexpression of candidates in model plants, and genetically engineering grapevine for selected genes.
Ten candidate genes were identified based on their presence in loci associated with bunch architecture traits, expression patterns during flower development, and sequence homology with genes regulating plant architecture in other species. These genes were cloned into plant expression vectors that were used to transform tomato and Arabidopsis thaliana plants. The research anticipates observing phenotypic evidence in the transformed model plants, which may suggest a potential role in grapevine bunch architecture regulation. The identification and characterisation of genes controlling bunch morphology offer promising avenues for advancing grapevine breeding and cultivation practices, benefiting the viticulture industry and consumers alike, by improving growth, health, and fruit production.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Kerry-Ann Jordaan1*, Manuela Campa1, Luca Nerva2, Johan Burger1, Justin Lashbrooke1

1Department of Genetics, Stellenbosch University, P/Bag X1, Matieland, 7602, South Africa
2Research Centre for Viticulture and Oenology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile, 31015 Conegliano (TV), Italy

Contact the author*

kerryaj@sun.ac.za

Keywords

bunch morphology, overexpression, grapevine, compactness, transformation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Beyond classical statistics – data fusion coupled with pattern recognition

AIM: Patterns in data obtained from wine chemical and sensory evaluations are difficult to infer using classical statistics.

Read More

Interest and impact of PVP/PVI (Polyvinylpyrrolidone/ Polyvinylimidazole) on winemaking and final quality of wines

Céline Sparrow a, Christophe Morge a, a SOFRALAB SAS, 79, av. A.A. Thévenet – CS 11031 – 51530 Magenta, France Consumers’ health and security force authorities to limit, in wine as in others food industry products, the concentration in « dangerous » molecules. Therefore the legal limit in heavy metals keeps on decreasing. As per proof EU regulation just decrease the stain concentration in wine from 0,2 to 0,15 mg/l. Certain changes , such as sodium arsenite treatment in vines, disappearance of brass in wineries to the benefit of stainless steel, limit even more the concentration of heavy metals in wines. But the use of copper derivates in vines treatments is difficult to replace. In the case of wine and its elaboration, the problem is even more complex. Indeed, regulation forces the wine producers to control the concentration of certain heavy metals in final wines.

Read More

Influence of social interaction levels on panel effectiveness in developing wine sensory profiles using consensus method

The development of sensory profiles is crucial for quality control and innovation in the wine industry. If quantitative descriptive analysis is the most commonly used method for establishing sensory profiles due to its robustness, it presents significant limitations.

Read More

Carbohydrate dynamics in Shiraz to determine seasonal allocation to the perennial and annual parts in respect to climatic challenges

The dynamic changes of non-structural carbohydrates (NSC) in grapevines during the growing season is driven by phenological events and environmental factors.

Read More

Georgian vitis germplasm: conservation, research and usage

Grapevine Vitis vinifera L. is a leader perennial crops for the Republic of Georgia, the South Caucasus. This is a region where the first wine making practice was initiated 8.000 years ago (McGovern et al. 2017) and a spot of grape domestication. The country of Georgia holds 525 local and more than 60 breeding varieties – they are preserved in 9 field collections inside the country.The list of recommended wine cultivars contains 34 names, including 27 old autochthonous varieties and covering 94% of the country’s vineyards.

Read More