terclim by ICS banner
IVES 9 IVES Conference Series 9 Functional characterisation of genetic elements regulating bunch morphology in grapevine

Functional characterisation of genetic elements regulating bunch morphology in grapevine

Abstract

Vitis vinifera L., is considered one of the world’s most important cultivated fruit crops. In agriculture, bunch morphology is a grapevine-specific trait, which directly impacts fruit quality and health.
Bunch size, shape, and compactness are major aspects of bunch morphology, with the degree of compactness emerging as an important trait for grapevine genetic enhancement and vineyard management. The importance of this trait stems from its impact on disease susceptibility, berry ripening, and other grape quality properties. However, current knowledge of the genes controlling it remains limited.
This study aims to identify and characterise genetic elements regulating grapevine bunch formation, while also providing valuable understanding of molecular and cellular regulation of this important process. This will be done by carrying out three planned objectives, summarised as: identification of candidate genes, overexpression of candidates in model plants, and genetically engineering grapevine for selected genes.
Ten candidate genes were identified based on their presence in loci associated with bunch architecture traits, expression patterns during flower development, and sequence homology with genes regulating plant architecture in other species. These genes were cloned into plant expression vectors that were used to transform tomato and Arabidopsis thaliana plants. The research anticipates observing phenotypic evidence in the transformed model plants, which may suggest a potential role in grapevine bunch architecture regulation. The identification and characterisation of genes controlling bunch morphology offer promising avenues for advancing grapevine breeding and cultivation practices, benefiting the viticulture industry and consumers alike, by improving growth, health, and fruit production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Kerry-Ann Jordaan1*, Manuela Campa1, Luca Nerva2, Johan Burger1, Justin Lashbrooke1

1Department of Genetics, Stellenbosch University, P/Bag X1, Matieland, 7602, South Africa
2Research Centre for Viticulture and Oenology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile, 31015 Conegliano (TV), Italy

Contact the author*

Keywords

bunch morphology, overexpression, grapevine, compactness, transformation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].

Techniques to study graft union formation in grapevine

Grapevines are grown grafted in most viticultural regions. Grapevine rootstocks are either hybrids or pure species of different American Vitis spp. (particularly V. berlandieri, V. rupestris and V. riparia), which were primarily used to provide root resistance to the insect pest Phylloxera. In addition to Phylloxera resistance, grapevine rootstocks were also selected in relation their resistance to various abiotic stress conditions. Future rootstocks should have the potential to adapt viticulture to climate change without changing the characteristics of the harvested product. However, high grafting success rates are an essential prerequisite to be able to use them with all the varieties. The objective of this work is to develop quantitative techniques to characterize graft union formation in grapevine.

Analysis of mousy off-flavour wines

Winemakers are increasingly experimenting with new techniques, such as spontaneous fermentation, prolonged yeast contact, higher pH, minimal sulphur dioxid, filtration and clarification or oxidative ageing. Along with this, the risk of microbial spoilage increases, and so the off-flavour mousiness, long time underestimated, is becoming more frequent. Characteristic of the mousy off-flavour is the delayed perception after swallowing the wine. After a few seconds the flavour appears, reminiscent of a dirty mouse cage. There are three known compounds that cause mousy off-flavor: 2-ethyltetrahydropyridine, 2-acetyltetrahydopyridine, and 2-acetylpyrroline. Yeasts such as Dekkera/Brettanomyces and heterofermentative lactic acid bacteria like Lactobacillus hilgardii can release these compounds.

La variabilità del colore in vini rosati dell’Italia meridionale

Nei vini rosati, è il colore ad avere il primo impatto con il consumatore. Esso risulterà tanto più accattivante, quanto più elegante e raffinato si presenta.

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution.