terclim by ICS banner
IVES 9 IVES Conference Series 9 Functional characterisation of genetic elements regulating bunch morphology in grapevine

Functional characterisation of genetic elements regulating bunch morphology in grapevine

Abstract

Vitis vinifera L., is considered one of the world’s most important cultivated fruit crops. In agriculture, bunch morphology is a grapevine-specific trait, which directly impacts fruit quality and health.
Bunch size, shape, and compactness are major aspects of bunch morphology, with the degree of compactness emerging as an important trait for grapevine genetic enhancement and vineyard management. The importance of this trait stems from its impact on disease susceptibility, berry ripening, and other grape quality properties. However, current knowledge of the genes controlling it remains limited.
This study aims to identify and characterise genetic elements regulating grapevine bunch formation, while also providing valuable understanding of molecular and cellular regulation of this important process. This will be done by carrying out three planned objectives, summarised as: identification of candidate genes, overexpression of candidates in model plants, and genetically engineering grapevine for selected genes.
Ten candidate genes were identified based on their presence in loci associated with bunch architecture traits, expression patterns during flower development, and sequence homology with genes regulating plant architecture in other species. These genes were cloned into plant expression vectors that were used to transform tomato and Arabidopsis thaliana plants. The research anticipates observing phenotypic evidence in the transformed model plants, which may suggest a potential role in grapevine bunch architecture regulation. The identification and characterisation of genes controlling bunch morphology offer promising avenues for advancing grapevine breeding and cultivation practices, benefiting the viticulture industry and consumers alike, by improving growth, health, and fruit production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Kerry-Ann Jordaan1*, Manuela Campa1, Luca Nerva2, Johan Burger1, Justin Lashbrooke1

1Department of Genetics, Stellenbosch University, P/Bag X1, Matieland, 7602, South Africa
2Research Centre for Viticulture and Oenology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile, 31015 Conegliano (TV), Italy

Contact the author*

Keywords

bunch morphology, overexpression, grapevine, compactness, transformation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

REDWINE project: use of Chlorella vulgaris to prevent biotic and abiotic stress in Palmela’s region, Portugal, vineyards

The new EU Green Deal aims to achieve GHG emissions reduction by at least 55% by 2030 and a climate neutral EU economy by 2050.
REDWine concept will be realized through the establishment of an integrated Living Lab demonstrating the viability of the system at TRL 7. The Living Lab will be able to utilize 2 ton of fermentation off-gas/year (90% of total CO2 produced in the fermenter) and 80 m3 of liquid effluent (100% of the liquid effluent generated during fermenter washing) to produce 1 ton (dry weight) of Chlorella biomass/year. This biomass will be processed under a downstream extraction process to obtain added-value extracts and applied in food, cosmetic and agricultural end-products and to generate a new EcoWine. REDWine will focus on the recovery of off-gas from a 20.000L fermenter of red wine production existing in Adega Cooperativa de Palmela (ACP, located in Palmela, Portugal).

The effect of wine cork closures on volatile sulfur compounds during accelerated post-bottle ageing in Shiraz wines

Reduced off-flavour is an organoleptic defect due to an excess of volatile sulfur compounds (VSC) in wine and often happening in Shiraz wines. This off-flavour is a direct consequence of the lack of oxygen flow during winemaking and bottle storage. Therefore, wine closure could have a direct impact on the formation of VSC due to the oxygen transfer rate that can modulate their levels. Even if dimethylsulfide (DMS) contributes to reduced off-flavor, it is also a fruity note enhancer in wine and its evolution during wine ageing is not well understood.

Evaluation of climate change impacts at the Portuguese Dão terroir over the last decades: observed effects on bioclimatic indices and grapevine phenology

In the last decades the growers of the Portuguese Dão winegrowing region (center of Portugal) are experiencing changes in climate that are influencing either grape phenology berry health and ripening. Aiming to study the relationships between climate indices (CI), seasonal weather and grapevine phenology, in this work long-term climate and phenological data collected at the experimental vineyard of the Portuguese Dão research centre between 1958 and 2019 (61 years) for the red variety Touriga Nacional, was analyzed. The trends over time for the classical temperature-based indices (Growing Season Temperature – GST -, Growing Degree Days – GDD, Huglin Index – HI and Cool Night Index – CI) presented a significantly positive slope while the Dryness Index (DI) showed a negative trend over the last 61 years. Regarding grapevine phenology, an average advance of 4.5 days per decade in the harvest day was observed throughout the last 61 years. Consequently, the weather conditions during the ripening period have changed, showing an increasing trend over time in the average temperature (higher magnitude in the maximum than in the minimum temperature) and a decrease in the accumulated rainfall. A regression analysis showed that ~50% of harvest date variability over years was explained by the temperature-based indices variability. These observed effects of climate change on bioclimatic indices and corresponding anticipation of harvest date can still be considered advantageous for the Dão terroir as it allows to achieve an optimal berry ripening before the common equinox rains and, therefore, avoid the potential negative impacts of the rainfall on berry health and composition.

Permanent vs temporary cover crops in a Sangiovese vineyard: preliminary results on vine physiology and productive traits

Cover crops in vineyards have been extensively studied, as the choice of grass species and their management significantly influence soil properties and vine performance.

Vineyard Landscape in Vale dos Vinhedos: Pressure and Protection

The region with Origin Indication Vale dos Vinhedos (IPVV) is located in the cities of Bento Gonçalves, Garibaldi and Monte Belo do Sul. It was established in November 2002, through an act of Instituto Nacional de Propriedade Industrial, according to Resolução INPI Nº. 75, of 2000, and Law Nº. 9.279 of 1996. The changes on the grape growing and wine making in the last 15 years, with the expansion of grapevines Vitis viníferas cultivation, the installation of several modern family wine companies, making wine with competitive quality, among other aspects, have enabled the implementation of concepts of Geographical Indications.