terclim by ICS banner
IVES 9 IVES Conference Series 9 Functional characterisation of genetic elements regulating bunch morphology in grapevine

Functional characterisation of genetic elements regulating bunch morphology in grapevine

Abstract

Vitis vinifera L., is considered one of the world’s most important cultivated fruit crops. In agriculture, bunch morphology is a grapevine-specific trait, which directly impacts fruit quality and health.
Bunch size, shape, and compactness are major aspects of bunch morphology, with the degree of compactness emerging as an important trait for grapevine genetic enhancement and vineyard management. The importance of this trait stems from its impact on disease susceptibility, berry ripening, and other grape quality properties. However, current knowledge of the genes controlling it remains limited.
This study aims to identify and characterise genetic elements regulating grapevine bunch formation, while also providing valuable understanding of molecular and cellular regulation of this important process. This will be done by carrying out three planned objectives, summarised as: identification of candidate genes, overexpression of candidates in model plants, and genetically engineering grapevine for selected genes.
Ten candidate genes were identified based on their presence in loci associated with bunch architecture traits, expression patterns during flower development, and sequence homology with genes regulating plant architecture in other species. These genes were cloned into plant expression vectors that were used to transform tomato and Arabidopsis thaliana plants. The research anticipates observing phenotypic evidence in the transformed model plants, which may suggest a potential role in grapevine bunch architecture regulation. The identification and characterisation of genes controlling bunch morphology offer promising avenues for advancing grapevine breeding and cultivation practices, benefiting the viticulture industry and consumers alike, by improving growth, health, and fruit production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Kerry-Ann Jordaan1*, Manuela Campa1, Luca Nerva2, Johan Burger1, Justin Lashbrooke1

1Department of Genetics, Stellenbosch University, P/Bag X1, Matieland, 7602, South Africa
2Research Centre for Viticulture and Oenology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile, 31015 Conegliano (TV), Italy

Contact the author*

Keywords

bunch morphology, overexpression, grapevine, compactness, transformation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Use of the stics crop model as a tool to inform vineyard zonages

STICS est un modèle de culture développé à l’INRA (France) depuis 1996. Il simule les bilans de carbone, d’eau et d’azote dans le système culture-sol, piloté par des données climatiques journaliéres. Il calcule à la fois des variables agricoles (rendement en quantité et qualité) et environnementales (pertes en eau et en azote). Une des originalités de STICS est son adaptabilité à de nombreuses cultures (herbacées, ligneuses, annuelles, pérennes) rendue possible par le choix de paramètres génériques et d’options de formalismes. Le travail présenté traite, dans un premier temps, des spécificités de STICS pour la vigne en terme de bilan trophique, de fonctionnement énergétique et hydrique et d’estimation des teneurs en sucre en en eau du raisin. Nous montrons ensuite diverses sorties du modèle qui permettent de caractériser des terroirs du vignoble des Côtes du Rhône.

Improving stilbenes in vitis Labrusca L. Grapes through methyl jasmonate applications

Grapes (Vitis sp.) are considered a major source of phenolic compounds such as flavonols, anthocyanins and stilbenes. Studies related to the beneficial effects of these compounds on health have encouraged research aimed at increasing their concentration in fruits. On this behalf, several plant growth regulators such as jasmonic acid and its volatile ester, methyl-jasmonate (MeJa), have demonstrated promising results in many fruits. However, Brazilian subtropical climate might interfere on treatment response. The present study aims to evaluate the application of MeJa in the pre-harvest period in Concord and Isabel Precoce grapes (Vitis labrusca L.).

Rootstock mediated responses of grapevine (Vitis vinifera L.) metabolism and physiology to combined water deficit and salinity stress in Syrah grafts

Water deficit and salinity are increasingly affecting the viticulture and wine industry. These two stresses are intimately related; understanding the physiological and metabolic responses of grapevines to water deficit, salinity and combined stress is critical for developing strategies to mitigate the nega- tive impacts of these stresses on wine grape production. These strategies can include selecting more tolerant grapevine cultivars and graft combinations, improving irrigation management, and using soil amendments to reduce the effects of salinity. For this purpose, understanding the response of grape- vine metabolism to altered water balance and salinity is of pivotal importance.

Towards a better understanding of the root system diversity and plasticityin young grafted vines using 2D imaging and 3D modelling tools

Three-dimensional functional-structural root architecture models, which decompose the root system architecture (RSA) into elementary developmental processes such as root emission, axial growth, branching patterns and tropism have become useful tools for (i) reconstructing in silico the spatial and temporal dynamics of root systems in a soil volume, (ii) analyzing their genotypic diversity and plasticity to the environment, and (iii) overcoming the bottleneck associated with their visualization and measurement in situ. Here, we present an original work on RSA phenotyping and modelling in grapevine. First, we developed 2D image-based analysis pipelines to quantify morphological and architectural traits in young grafts. Second, we parametrized and validated the 3D root model Archisimple on two rootstock genotypes (RGM, 1103P) grafted with V. vinifera Cabernet-Sauvignon and grown in different controlled conditions (rhizotrons, pots, tubes).

The effect of water stress deficit on ‘Xynisteri’ grapes through systems biology approaches

Cyprus is one of the very few phyloxera-free areas worldwide where the vast majority of vines are own-rooted and non-irrigated. ‘Xynisteri’ is a predominant indigenous cultivar, particularly amenable to extreme conditions such as drought and hot climate, thus rendering it appropriate for marginal soils and adverse climatic conditions. In the current work, a comparative study between irrigated (irrigation initiated at BBCH 71) and non-irrigated vines was conducted.