terclim by ICS banner
IVES 9 IVES Conference Series 9 Functional characterisation of genetic elements regulating bunch morphology in grapevine

Functional characterisation of genetic elements regulating bunch morphology in grapevine

Abstract

Vitis vinifera L., is considered one of the world’s most important cultivated fruit crops. In agriculture, bunch morphology is a grapevine-specific trait, which directly impacts fruit quality and health.
Bunch size, shape, and compactness are major aspects of bunch morphology, with the degree of compactness emerging as an important trait for grapevine genetic enhancement and vineyard management. The importance of this trait stems from its impact on disease susceptibility, berry ripening, and other grape quality properties. However, current knowledge of the genes controlling it remains limited.
This study aims to identify and characterise genetic elements regulating grapevine bunch formation, while also providing valuable understanding of molecular and cellular regulation of this important process. This will be done by carrying out three planned objectives, summarised as: identification of candidate genes, overexpression of candidates in model plants, and genetically engineering grapevine for selected genes.
Ten candidate genes were identified based on their presence in loci associated with bunch architecture traits, expression patterns during flower development, and sequence homology with genes regulating plant architecture in other species. These genes were cloned into plant expression vectors that were used to transform tomato and Arabidopsis thaliana plants. The research anticipates observing phenotypic evidence in the transformed model plants, which may suggest a potential role in grapevine bunch architecture regulation. The identification and characterisation of genes controlling bunch morphology offer promising avenues for advancing grapevine breeding and cultivation practices, benefiting the viticulture industry and consumers alike, by improving growth, health, and fruit production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Kerry-Ann Jordaan1*, Manuela Campa1, Luca Nerva2, Johan Burger1, Justin Lashbrooke1

1Department of Genetics, Stellenbosch University, P/Bag X1, Matieland, 7602, South Africa
2Research Centre for Viticulture and Oenology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile, 31015 Conegliano (TV), Italy

Contact the author*

Keywords

bunch morphology, overexpression, grapevine, compactness, transformation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Variability of Tempranillo phenology within the toro do (Spain) and its relationship to climatic characteristics

Aims: The objective of this research was to analyse the spatial and temporal variability of vine phenology of the Tempranillo variety in the Toro Designation of Origen (DO) related to climatic conditions at present and under future climate change scenarios.

Future projections for chilling and heat forcing for European vineyards

Aims: The aims of this study were: (1) to compute recent-past thermal conditions over European vineyards, using state-of-the art bioclimatic indices: chilling portions and growing degree hours; (2) to compute future changes of these thermal conditions using a large ensemble of high-resolution climate models.

Grapevine nitrogen status: correlation between chlorophyll indices n-tester and spadGrapevine nitrogen status

Knowledge of the nitrogen nutrition status of grapevines is essential for the sustainable management of their nutrition for the production of quality grapes. The measurement of the chlorophyll index is a rapid, non-destructive and relatively inexpensive method that provides a good approximation of the nitrogen nutrition status of the vine during the season. Interpretation thresholds are currently insufficient or non-existent for some chlorophyll meters. Ideally, they should be available for each variety and each phenological stage. In order to popularize the use of chlorophyll-meters, measurements were carried out at Agroscope in Switzerland to establish the correlation between the indices obtained by the devices N-tester and SPAD 502.

Influence of protective colloids on tartrate stability, polysaccharide contents and volatile compound profile of a white wine

The tartaric salts precipitation is one of the main issues regarding wine instability 1. In addition to the well-known and deeply studied phenomena of potassium hydrogentartrate precipitation (KHT), the last decade has been increased the phenomena of calcium tartrate (CaT) precipitation, that is a concern for the wine industry 2.

Berry weight loss in Vitis vinifera (L.) cultivars during ripening

Berry shriveling (BS) in vineyards are caused by numerous factors such as sunburn, dehydration, stem necrosis. Climate change results in an increase in day and night temperatures, rainfall throughout the year, changes in the timing and quantities, long dry summers and a combination of climatic variability such as floods, droughts and heatwaves). Grape development and its composition at harvest is influenced by the latter as grape metabolites are sensitive to the environmental conditions. The grape berry experiences water loss and an increase in flavour development as a result of the BS. An increased sugar content in grapes will result in higher alcohol wines and concentration of grape aromas which may be detrimental to the final wine quality.