terclim by ICS banner
IVES 9 IVES Conference Series 9 Comparative QTL mapping of phenology traits in three cross populations of grapevine

Comparative QTL mapping of phenology traits in three cross populations of grapevine

Abstract

Long-term studies on grapevine phenology have clearly demonstrated that global warming is affecting phenological events, leading to an anticipation in their timing, and negatively impacting grape yield and berry quality. Therefore, dissecting the genetic determinants involved in the plant regulation of the phenological stages of budburst, flowering, veraison and ripening can improve our knowledge of the underlying mechanisms and support plant breeding programs and the advancement of vineyard management strategies.
We report here the results of a QTL mapping experiment conducted on three segregating populations obtained from the crossing of ‘Cabernet Sauvignon’ and ‘Corvina’, ‘Corvina’ and the hybrid ‘Solaris’ and ‘Rhine Riesling’ and ‘Cabernet Sauvignon’. High-density parental and integrated linkage maps were developed by using genotypic information, obtained through hybridization to the Illumina Vitis18KSNP chip, of DNA from 144, 129 and 139 individuals respectively. Each progeny was then evaluated in the field over four seasons. The phenological traits budburst, flowering, veraison as well as technological ripening were assessed, and correlations across years and traits were estimated. A summary of all mapped QTLs in the different years in each population is provided and QTLs reproducible across years and populations as well as potential underlying candidates are discussed.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Martina Marini1, Laura Costantini2, Silvia Pettenuzzo2,3,4, Silvia Lorenzi2 ,Tahir Mujtaba1, Riccardo Mora1, Ron Shmuleviz1,Giovanni Battista Tornielli1,5, Giada Bolognesi1, Maria Stella Grando3, Diana Bellin1

1 Department of Biotechnology, University of Verona, Italy
2Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
3Center Agriculture Food and Environment (C3A), University of Trento, Italy
4Department of Chemical Sciences, University of Padova, Italy
5Current address: Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Italy

Contact the author*

Keywords

climate change, phenology, cross populations, QTL mapping, candidate genes

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

EFFECT OF FUMARIC ACID ON SPONTANEOUS FERMENTATION IN GRAPE MUST

Malolactic fermentation (MLF)¹, the decarboxylation of L-malic acid into L-lactic acid, is performed by lactic acid bacteria (LAB). MLF has a deacidifying effect that may compromise freshness or microbiological stability in wines² and can be inhibited by fumaric acid [E297] (FA). In wine, can be added at a maximum allowable dose of 0.6 g/L³. Its inhibition with FA is being studied as an alternative strategy to minimize added doses of SO₂⁴. In addition, wine yeasts are capable of metabolizing and storing small amounts of FA and during alcoholic fermentation (AF).

Effect of regulated deficit irrigation regime on amino acids content of Monastrell (Vitis vinifera L.) grapes

Irrigation is an important practice to influence vine quality, especially in Mediterranean regions, characterized by hot summers and severe droughts during the growing season. This study focused on deficit irrigation regime influence on amino acids composition of Monastrell grapevines under semiarid conditions (Albacete, Southeastern of Spain). In 2019, two treatments were applied: non-irrigation (NI) and regulated deficit irrigation (RDI), watered at 30% of the estimated crop evapotranspiration from fruit set to onset of veraison. Grape amino acids content was analyzed by HPLC. Berries from non-irrigated vines showed higher concentration of several amino acids, such as tryptophan (73%), arginine (70%), lysine (36%), isoleucine (27%), and leucine (21%), compared to RDI grapes. Arginine is, together with ammonium ion, the principal nitrogen source for yeasts during the alcoholic fermentation; while isoleucine, tryptophan, and leucine are precursors of fermentative volatile compounds, key compounds for wine quality. Moreover, NI treatment increased in a 14% the total amino acids content in grapes compared to RDI treatment. The reported effects might be because yield was 70% higher in RDI vines than in the NI ones and, therefore, the sink demand was increased in the irrigated vines. In addition, NI vines suffered more severe water stress and it is known that the amino acids synthesis and accumulation can be influenced by the plant response to stress. According to the results, the irrigation regime showed effect on amino acids concentration in Monastrell grapes under semiarid conditions. Grapes from non-irrigated vines showed a higher content of several amino acids relevant to the fermentative process and to the wine aroma compounds formation. It is demonstrated that the final content of nitrogen-related components in grapes is influenced by the irrigation regime. The convenience of the irrigation strategy to suggest will depend on the desired wine style and the target yield levels.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Grapevine is grown as a graft since the end of the 19th century. Rootstocks not only provide tolerance to Phylloxera but also ensure the supply of water and mineral nutrients to the scion. Rootstocks are an important mean of adaptation to environmental conditions, because the scion controls the typical features of the grapes and wine. However, among the large diversity of rootstocks worldwide, few of them are commercially used in the vineyard. The aim of this study was to investigate the extent to which rootstocks modify the mineral composition of the petioles of the scion. Vitis vinifera cvs. Cabernet-Sauvignon, Pinot noir, Syrah and Ugni blanc were grafted onto 55 different rootstock genotypes and planted in a vineyard as three replicates of 5 vines. Petioles were collected in the cluster zone with 6 replicates per combination. Petiolar concentrations of 13 mineral elements (N, P, K, S, Mg, Ca, Na, B, Zn, Mn, Fe, Cu, Al) at veraison were determined. Scion, rootstock and the interaction explained the same proportion of the phenotypic variance for most mineral elements. Rootstock genotype showed a significant influence on the petiole mineral element composition. Rootstock effect explained from 7 % for Cu to 25 % for S of the variance. The difference of rootstock conferred mineral status is discussed in relation to vigor and fertility. Rootstocks were also genotyped with 23 microsatellite markers. Data were analysed according to genetic groups in order to determine whether the petiole mineral composition could be related to the genetic parentage of the rootstock. Thanks to a highly powerful design, it is the first time that such a large panel of rootstocks grafted with 4 scions has been studied. These results give the opportunity to better characterize the rootstocks and to enlarge the diversity used in the vineyard.

Tuning the pH during the fermentation has a strong effect on the wine protein composition and the stability of the resulting white wines

Previous results have shown the impact of the pH on the stability of white wine proteins. In a context of global warming that implies increases in ethanol content and pH

Vine nitrogen status and the terroir effect: a study on cv. Doral in the Vaud vineyard (Switzerland)

A 3-year study was conducted in the Vaud vineyard (Switzerland) to evaluate the effects of « terroir » on the ecophysiology and fruit compostion of Vitis vinifera L. cv.