terclim by ICS banner
IVES 9 IVES Conference Series 9 Comparative QTL mapping of phenology traits in three cross populations of grapevine

Comparative QTL mapping of phenology traits in three cross populations of grapevine

Abstract

Long-term studies on grapevine phenology have clearly demonstrated that global warming is affecting phenological events, leading to an anticipation in their timing, and negatively impacting grape yield and berry quality. Therefore, dissecting the genetic determinants involved in the plant regulation of the phenological stages of budburst, flowering, veraison and ripening can improve our knowledge of the underlying mechanisms and support plant breeding programs and the advancement of vineyard management strategies.
We report here the results of a QTL mapping experiment conducted on three segregating populations obtained from the crossing of ‘Cabernet Sauvignon’ and ‘Corvina’, ‘Corvina’ and the hybrid ‘Solaris’ and ‘Rhine Riesling’ and ‘Cabernet Sauvignon’. High-density parental and integrated linkage maps were developed by using genotypic information, obtained through hybridization to the Illumina Vitis18KSNP chip, of DNA from 144, 129 and 139 individuals respectively. Each progeny was then evaluated in the field over four seasons. The phenological traits budburst, flowering, veraison as well as technological ripening were assessed, and correlations across years and traits were estimated. A summary of all mapped QTLs in the different years in each population is provided and QTLs reproducible across years and populations as well as potential underlying candidates are discussed.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Martina Marini1, Laura Costantini2, Silvia Pettenuzzo2,3,4, Silvia Lorenzi2 ,Tahir Mujtaba1, Riccardo Mora1, Ron Shmuleviz1,Giovanni Battista Tornielli1,5, Giada Bolognesi1, Maria Stella Grando3, Diana Bellin1

1 Department of Biotechnology, University of Verona, Italy
2Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
3Center Agriculture Food and Environment (C3A), University of Trento, Italy
4Department of Chemical Sciences, University of Padova, Italy
5Current address: Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Italy

Contact the author*

Keywords

climate change, phenology, cross populations, QTL mapping, candidate genes

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Evolution of the metabolic profile of grapes in a context of climate change

In the current context of global climate change, anticipating the evolution of the oenological potential of emblematic grape varieties of regions such as Burgundy and Champagne is a guarantee of the sustainability of a sector which has considerable economic weight. however, if various models of climate change cast doubt on the sustainability of these grape varieties in these regions, appellation decrees, as well as consumer expectations, do not allow or consider the use of alternative grape varieties. In addition, control/compensation methods such as irrigation are also not permitted.

Delaying irrigation initiation linearly reduces yield with little impact on maturity in Pinot noir

When to initiate irrigation is a critical annual management decision that has cascading effects on grapevine productivity and wine quality in the context of climate change. A multi-site trial was begun in 2021 to optimize irrigation initiation timing using midday stem water potential (ψstem) thresholds characterized as departures from non-stressed baseline ψstemvalues (Δψstem). Plant material, vine and row spacing, and trellising systems were concomitant among sites, while vine age, soil type, and pruning systems varied. Five target Δψstem thresholds were arranged in an RCBD and replicated eight times at each site: 0.2, 0.4, 0.6, 0.8, and 1.0 MPa (T1, T2, T3, T4, and T5, respectively). When thresholds were reached, plots were irrigated weekly at 70% ETc. Yield components and berry composition were quantified at harvest. To better generalize inferences across sites, data were analyzed by ANOVA using a mixed model including site as a random factor. Across sites, irrigation was initiated at Δψstem = 0.24, 0.50, 0.65, 0.93, and 0.98 MPa for T1, T2, T3, T4, and T5, respectively. Consistent significant negative linear trends were found for several key yield and berry composition variables. Yield decreased by 12.9, 15.9, 19.5, and 27.4% for T2, T3, T4, and T5, respectively, compared to T1 (p < 0.0001) across sites that were driven by similarly linear reductions in berry weight (p < 0.0001). Comparatively, berry composition varied little among treatments. Juice total soluble solids decreased linearly from T1 to T5 – though only ranged 0.9 Brix (p = 0.012). Because producers are paid by the ton, and contracts simply stipulate a target maturity level, first-year results suggest that there is no economic incentive to induce moderate water deficits before irrigation initiation, regardless of vineyard site. Subsequent years will further elucidate the carryover effects of delaying irrigation initiation on productivity over the long term.

Which potential for Near Infrared Spectroscopy to characterize rootstock effects on grapevines?

Developing rootstocks adapted to environmental constraints constitutes a key lever for grapevine adaptation to climate change. In this context, Near Infrared Spectroscopy (NIRS) could be used as a high-throughput phenotyping technique to simplify the study of rootstocks in grafted situations. This study is an exploratory analysis to evaluate the potential of NIRS acquired on grafted tissues to reveal rootstock effects as well as the plasticity of combinations of scion/rootstock to better characterize these interactions.
Through the study of 25 combinations (5 scions times 5 rootstocks) in a dedicated experimental vineyard, we showed that NIRS obtained from grafted tissues capture rootstock and scion/rootstock interaction signals, up to 20% of the total variance at specific wavelengths.

Distribution of fungicide-resistant Botrytis cinerea mutations in the Tokaj and Eger wine regions

Botrytis cinerea is one of the most widespread host-specific fungal pathogens, causing significant yield losses and economic damage to vineyards every year.

La haie bocagère comme critère de zonage à l’échelle parcellaire

In the French AOCs, the production area of ​​the raw material can be subject to plot delimitation based on criteria of physical environment and use. On the other hand, many environmental zonings are developing and the AOCs are called upon include provisions relating to these concerns. Hedges, through their effects on local changes in the regional climate and on functional biodiversity, can impact the functioning of vines and orchards. It is for this reason that their consideration as a delimitation criterion is envisaged.