Ugni blanc berry and wine composition impacted by thirteen rootstocks

A study of soil pH on the experimental field resulted in a high variability of pH on a very small scale. This kind of heterogenity in soil pH have effects on growth of two grapevine varieties on rootstock Kober 5BB

Wine storage ensuring the quality and correct aging is one of the issues that wineries, wine traders and consumers encounter after wine bottling.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

Microbial pathogens of plant have evolved to sense, interpret, and use light to direct their development. One aspect of this evolved relationship is photolyase-mediated repair of UV-induced damage to pathogen DNA. Application of germicidal UV (UV-C) at night circumvents the blue light-driven repair of pathogen DNA and allows non-phytotoxic doses of UV-C to suppress a variety of pathogenic microbes and even certain arthropod pests without damage to vines or fruit. Lamps arrays have been designed specifically for the canopy architecture of grapevines and have been deployed on both tractor-drawn and robotic carriages for partial to near-complete suppression of powdery mildew (Erysiphe necator), sour rot (fungal, bacterial, and arthropod complex), and downy mildew (Plasmopara viticola).

Grape volatile compounds are mainly responsible for wine aroma, so it is important to know the va-rietal aromatic composition throughout ripening process. Currently, there are no tools that allow mea-suring the aromatic composition of grapes, in intact berries and periodically, throughout ripening, in the vineyard or in the winery. For this reason, this work evaluated the use of near infrared spectroscopy (NIR) to estimate the aromatic composition and total soluble solids (TSS) of Tempranillo Blanco berries during ripening. For this purpose, NIR spectra (1100-2100 nm) were acquired from 240 samples of in-tact berries, collected at different dates, from veraison to overripening.