terclim by ICS banner
IVES 9 IVES Conference Series 9 The regulation of ABA-induced anthocyanin accumulation in grape berry

The regulation of ABA-induced anthocyanin accumulation in grape berry

Abstract

Color is a key quality trait for grape berry and the producing wines. Berry color of red genotypes is mainly determined by the quantity and composition of anthocyanins accumulated in the skin and/or pulp. Both genetic and environmental factors could influence the quantity and composition of anthocyanins, while the underlying mechanisms are not fully clear. To explore the mechanisms underlying the diversity of anthocyanin accumulation in grape berry, we compared two grapevine genotypes showing distinct sensitivities to ABA-induced anthocyanin biosynthesis, where one genotype showed minor responses to exogenous ABA application while the other showed significant increase in anthocyanins after exogenous ABA application. Transcriptome and metabolome were conducted and their analysis pointed out that the cis-element of MYBA1 might be responsible for the observed phenotypes. The promoters of MYBA1 were then cloned from both genotypes and several differences in their sequences were observed, but without any mutations in the ABRE elements. Dual-luciferase assay was applied to test the promoter activity and their responses to ABA, with a series of fragmented promoters of MYBA1 from both genotypes. The mutations in a portion of promoter not containing any ABREs were identified as the core for determining the sensitivity of ABA-induced anthocyanin accumulation. These results show the importance of sequence context on the function of cis-element and provide novel insights into the understanding of the mechanisms underlying the diversity of anthocyanin accumulation in grape berry.
Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Yibei Wang1,2, Junhua Kong1,2, Yongjian Wang1,2, Haiqi Wang1,2, Xiaobo Xu1,2, Boxing Shang1,2, Peige Fan1,2, Zhenchang Liang1,2, Zhanwu Dai1,2*

1 State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2 China National Botanical Garden, Beijing 100093, China

Contact the author*

Keywords

color, cis-regulation, sequence context, anthocyanin diversity, metabolism

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Quantification of polysaccharides of variety Pomaces of the D.O.Ca Rioja

Pomace is one of the main residues generated by the wine industry and represents an environmental problem. Currently, there is a growing interest in the revaluation of these products because different bioactive compounds can be obtained from them, such as polyphenols, grape seed oils and polysaccharides. Red grape pomace can be an important source of polysaccharides, but they are currently little studied and even less with viable and environmental extraction processes (green extraction), such as flash extraction. The residual amount of the fraction rich in pectin (residual pulp) and component rich in hemicellulose in the pomace and the strength of association of the pectin with the cellulose-xyloglucan network depend on the degree of extractability of the polysaccharides in red winemaking and on the winemaking conditions.

Read More

THE EFFECT OF BENTONITE FINING ON THE VOLATILE AND NON-VOLATILE PROFILE OF ITALIAN WHITE WINES

Marselan wines have an unusual high proportion of seed derived tannins from grapes having high proportions of skins, which are rich in tannins. But the causes behind this characteristic have not yet been identified. In vintage 2023 wines were made at experimental scale (9 kg by experimental unit) from Arinarnoa, Marselan and Tannat Vitis vinifera grape cultivars by traditional maceration, and by techniques aimed to increase the wine content in skin derived tannin: addition of extraction enzymes, addition at vatting of grape-skin enological tannins, or by extended maceration, known to increase the seed derived tannin contents of wines. Macerations were of 7 days, except in the extended macerations that were of 15 days.

Read More

Gambellara zoning: climate and soil effect on the aromatic fresh and dried grape composition and wine aroma

La région de production de la Gambellara et Recioto di Gambellara DOC (variété Garganega), tout en n’intéressant qu’une surface limitée, présente une certaine variabilité de milieu due à la morphologie du territoire (colline et plaine), à l’état actuel des sols et aux variations climatiques entre les différents sites. Pour les années 2001, 2002

Read More

Yeast interactions in chardonnay wine fermentation: impact of different yeast species using ultra high resolution mass spectrometry

During alcoholic fermentation, when yeasts grow simultaneously, they often do not coexist passively and in most cases interact with each others

Read More

Decline of new vineyards in Southern Spain

In-season vineyard pest management relies on proper timing, selection, and application of products. Most of the research on pest management tends to focus on the influence of regional conditions on these aspects, with an emphasis on product timing and efficacy evaluation. One aspect that is not fully vetted in various vineyard regions is application (sprayer) technology. The purpose of this study was to determine the influence of regional conditions on sprayer performance in commercial wine grape vineyards in eastern Washington.

Read More