terclim by ICS banner
IVES 9 IVES Conference Series 9 The regulation of ABA-induced anthocyanin accumulation in grape berry

The regulation of ABA-induced anthocyanin accumulation in grape berry

Abstract

Color is a key quality trait for grape berry and the producing wines. Berry color of red genotypes is mainly determined by the quantity and composition of anthocyanins accumulated in the skin and/or pulp. Both genetic and environmental factors could influence the quantity and composition of anthocyanins, while the underlying mechanisms are not fully clear. To explore the mechanisms underlying the diversity of anthocyanin accumulation in grape berry, we compared two grapevine genotypes showing distinct sensitivities to ABA-induced anthocyanin biosynthesis, where one genotype showed minor responses to exogenous ABA application while the other showed significant increase in anthocyanins after exogenous ABA application. Transcriptome and metabolome were conducted and their analysis pointed out that the cis-element of MYBA1 might be responsible for the observed phenotypes. The promoters of MYBA1 were then cloned from both genotypes and several differences in their sequences were observed, but without any mutations in the ABRE elements. Dual-luciferase assay was applied to test the promoter activity and their responses to ABA, with a series of fragmented promoters of MYBA1 from both genotypes. The mutations in a portion of promoter not containing any ABREs were identified as the core for determining the sensitivity of ABA-induced anthocyanin accumulation. These results show the importance of sequence context on the function of cis-element and provide novel insights into the understanding of the mechanisms underlying the diversity of anthocyanin accumulation in grape berry.
Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Yibei Wang1,2, Junhua Kong1,2, Yongjian Wang1,2, Haiqi Wang1,2, Xiaobo Xu1,2, Boxing Shang1,2, Peige Fan1,2, Zhenchang Liang1,2, Zhanwu Dai1,2*

1 State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2 China National Botanical Garden, Beijing 100093, China

Contact the author*

Keywords

color, cis-regulation, sequence context, anthocyanin diversity, metabolism

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Influence of irrigation on water status, productivity, yield and must composition in Tempranillo grapevine under Duero Valley zone conditions

Cette étude a pour but d’évaluer la modification de l’état hydrique (potentiel hydrique foliaire), le comportement productif (matière sèche et rendement) et la expression qualitative (poids de baie, degrée Brix, pH, acidité totale, concentration polyphénolique) de la varieté Tempranillo dans la Vallée du Douro, à l’A.O. Cigales, comme conséquence de l’application d’une irrigation modérée. Pour développer l’essai on a appliqué les suivantes

Read More

DETERMINATION OF MINERAL COMPOSITION IN CV. TERAN (VITIS VINIFERA L.) RED WINE AFFECTED BY PRE-FERMENTATIVE MASH COOLING, HEATING, SAIGNÉE TECHNIQUE AND PROLONGED POST-FERMENTATIVE MACERATIONS

This study aimed to determine mineral composition in red wine obtained from cv. Teran (Vitis vinifera L.), autochtonous Croatian grape variety. Six different vinification treatments, including the control treatment (7-day standard maceration), were performed to study the effects of: 48-hour pre-fermentative mash cooling (8 °C) followed by prolonged post-fermentative maceration of 13 days (C15), 28 days (C30), and saignée technique (juice runoff) proceeded with prolonged post-fermentative maceration of 13 days (CS15); and effect of 48-hour heating (50 °C) followed by prolonged post-fermentative maceration of 13 days (H15) and 28 days (H30) on macro- and microelements in wine.

Read More

Viticultural zoning of the country of Mendoza, Argentina. Study of the first zone : department of Luján de Cuyo. Statement of the study year 2002

La région viticole de Mendoza est la principale zone vitivinicole d’Argentine qui se compose de 3 oasis (Nord, Valle de Uco, Sud). La première zone vitivinicole, située dans l’oasis Nord, est composée par les département de Luján de Cuyo et Maipu. C’est la zone de production la plus ancienne et la plus reconnue pour la qualité de sa production. Ce travail se porte plus particulièrement sur le département de Luján de Cuyo qui constitue le lieu traditionnel de production du Malbec argertin. Ce travail propose de caractériser les terroirs et de mettre en avant leurs typicités.

Read More

Distribution analysis of myo and scyllo-inositol in natural grape must

s it is well known, myo and scyllo-inositol are two characteristic sugars of grape must and, for this reason, their quantification has been proposed to control the authenticity of the concentrated and rectificated grape must.

Read More

Experiments with the use of stems in Pinot noir winemaking

Vinification trials were carried out between 2018 and 2021 in the experimental winery at Laimburg Research Centre, Alto Adige, to test the effect of grape stem inclusion during fermentation of Pinot Noir.

Read More