terclim by ICS banner
IVES 9 IVES Conference Series 9 The regulation of ABA-induced anthocyanin accumulation in grape berry

The regulation of ABA-induced anthocyanin accumulation in grape berry

Abstract

Color is a key quality trait for grape berry and the producing wines. Berry color of red genotypes is mainly determined by the quantity and composition of anthocyanins accumulated in the skin and/or pulp. Both genetic and environmental factors could influence the quantity and composition of anthocyanins, while the underlying mechanisms are not fully clear. To explore the mechanisms underlying the diversity of anthocyanin accumulation in grape berry, we compared two grapevine genotypes showing distinct sensitivities to ABA-induced anthocyanin biosynthesis, where one genotype showed minor responses to exogenous ABA application while the other showed significant increase in anthocyanins after exogenous ABA application. Transcriptome and metabolome were conducted and their analysis pointed out that the cis-element of MYBA1 might be responsible for the observed phenotypes. The promoters of MYBA1 were then cloned from both genotypes and several differences in their sequences were observed, but without any mutations in the ABRE elements. Dual-luciferase assay was applied to test the promoter activity and their responses to ABA, with a series of fragmented promoters of MYBA1 from both genotypes. The mutations in a portion of promoter not containing any ABREs were identified as the core for determining the sensitivity of ABA-induced anthocyanin accumulation. These results show the importance of sequence context on the function of cis-element and provide novel insights into the understanding of the mechanisms underlying the diversity of anthocyanin accumulation in grape berry.
Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Yibei Wang1,2, Junhua Kong1,2, Yongjian Wang1,2, Haiqi Wang1,2, Xiaobo Xu1,2, Boxing Shang1,2, Peige Fan1,2, Zhenchang Liang1,2, Zhanwu Dai1,2*

1 State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2 China National Botanical Garden, Beijing 100093, China

Contact the author*

Keywords

color, cis-regulation, sequence context, anthocyanin diversity, metabolism

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Spatio-temporal analysis of grapevine water behaviour in hillslope vineyards. the example of corton hill, Burgundy

Hillslope vineyards show various and complex water dynamics between soil and plants, and in order to gain further insight into this phenomenon, 8 grapevine plots were monitored during three vintages, from 2010 to 2013, on Corton Hill, Burgundy, France.

Read More

Vineyard mulching offer many benefits beyond winter protection

Grapevines are susceptible to freezing damage at temperatures below -5°F during the winter season. Preventing winter injury to grapevines is a major challenge in many grape-producing regions. Conventional methods such as hilling-up soil over graft unions have been developed as winter protection methods for preventing vine loss. However, these practices have drawbacks such as soil erosion, vine damage and crown gall development.

Read More

Influence of Partial Rootzone Drying on grape and wine anthocyanin composition

The effect of Partial Rootzone Drying (PRD) on fruit and wine composition has been investigated. At harvest, total anthocyanin and phenolic concentration of Shiraz and Cabernet Sauvignon fruit was either unaltered or increased by PRD relative to control irrigation over two seasons. Where there was an increase in anthocyanin concentration

Read More

Rootstocks and climate change: adding up means learning faster

In this video recording of the IVES science meeting 2025, Gonzaga Santesteban (Public University of Navarra, Pamplona, Spain) speaks about rootstocks, climate change and meta-analysis. This presentation is based on an original article accessible for free on OENO One.

Read More

Changes in wine secondary metabolites composition by the timing of inoculation with lactic acid bacteria: impact on wine aroma

For the first time, it was established that the timing of inoculation with LAB could significantly impact the concentration of many secondary metabolites leading to significant aromatic changes. From studied compounds, the most influenced were esters and diacetyl.

Read More