terclim by ICS banner
IVES 9 IVES Conference Series 9 The regulation of ABA-induced anthocyanin accumulation in grape berry

The regulation of ABA-induced anthocyanin accumulation in grape berry

Abstract

Color is a key quality trait for grape berry and the producing wines. Berry color of red genotypes is mainly determined by the quantity and composition of anthocyanins accumulated in the skin and/or pulp. Both genetic and environmental factors could influence the quantity and composition of anthocyanins, while the underlying mechanisms are not fully clear. To explore the mechanisms underlying the diversity of anthocyanin accumulation in grape berry, we compared two grapevine genotypes showing distinct sensitivities to ABA-induced anthocyanin biosynthesis, where one genotype showed minor responses to exogenous ABA application while the other showed significant increase in anthocyanins after exogenous ABA application. Transcriptome and metabolome were conducted and their analysis pointed out that the cis-element of MYBA1 might be responsible for the observed phenotypes. The promoters of MYBA1 were then cloned from both genotypes and several differences in their sequences were observed, but without any mutations in the ABRE elements. Dual-luciferase assay was applied to test the promoter activity and their responses to ABA, with a series of fragmented promoters of MYBA1 from both genotypes. The mutations in a portion of promoter not containing any ABREs were identified as the core for determining the sensitivity of ABA-induced anthocyanin accumulation. These results show the importance of sequence context on the function of cis-element and provide novel insights into the understanding of the mechanisms underlying the diversity of anthocyanin accumulation in grape berry.
Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Yibei Wang1,2, Junhua Kong1,2, Yongjian Wang1,2, Haiqi Wang1,2, Xiaobo Xu1,2, Boxing Shang1,2, Peige Fan1,2, Zhenchang Liang1,2, Zhanwu Dai1,2*

1 State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2 China National Botanical Garden, Beijing 100093, China

Contact the author*

Keywords

color, cis-regulation, sequence context, anthocyanin diversity, metabolism

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Kinetic investigations of the sulfite addition on flavanols

Sulfonated monomeric and dimeric flavan-3-ols are recently discovered in wine and proved to have great importance in understanding wine chemistry and quality [1, 2].

Read More

Towards an ecological architecture inspired by underground cellars: An example of the thermal inertia of Moldovan underground cellars and new geothermal and Canadian well approaches

The search for underground shelters is one of the oldest forms of human habitation, providing refuge in extreme environments such as deserts and polar regions.

Read More

Aspect juridiques des terroirs

Le “terroir” est dans tous les discours, les articles, les étiquettes et les publicités. Le voca­ble est en situation d’utilisation euphorique. Indiscutablement l’emploi historique est agri­cole, puis viticole, mais il n’est jamais juridique.

Read More

Effect of terroir on the quality evolution of Cabernet-Sauvignon in Penedès A.0.C.

Le Cabernet-Sauvignon est un cépage très répandu dans la région du Penedès (Espagne) où cette variété peut bien s’adapter et donne des produits de haute qualité.

Read More

High pressure homogenization of wine lees. A tool to streamline the management of wine ageing

Aging on lees (AOL) has been used for wine aging for a long time, thanks to its ability to modify wine composition, improving sensory characteristics and stability. However, the prolonged contact with fermentation lees may increase the risk of developing sensory defects, due to the growth of unwanted microorganisms. Furthermore, AOL requires a large amount of work to manage bâtonnage and for topping up the barrels, significantly increasing production costs.

Read More