terclim by ICS banner
IVES 9 IVES Conference Series 9 The regulation of ABA-induced anthocyanin accumulation in grape berry

The regulation of ABA-induced anthocyanin accumulation in grape berry

Abstract

Color is a key quality trait for grape berry and the producing wines. Berry color of red genotypes is mainly determined by the quantity and composition of anthocyanins accumulated in the skin and/or pulp. Both genetic and environmental factors could influence the quantity and composition of anthocyanins, while the underlying mechanisms are not fully clear. To explore the mechanisms underlying the diversity of anthocyanin accumulation in grape berry, we compared two grapevine genotypes showing distinct sensitivities to ABA-induced anthocyanin biosynthesis, where one genotype showed minor responses to exogenous ABA application while the other showed significant increase in anthocyanins after exogenous ABA application. Transcriptome and metabolome were conducted and their analysis pointed out that the cis-element of MYBA1 might be responsible for the observed phenotypes. The promoters of MYBA1 were then cloned from both genotypes and several differences in their sequences were observed, but without any mutations in the ABRE elements. Dual-luciferase assay was applied to test the promoter activity and their responses to ABA, with a series of fragmented promoters of MYBA1 from both genotypes. The mutations in a portion of promoter not containing any ABREs were identified as the core for determining the sensitivity of ABA-induced anthocyanin accumulation. These results show the importance of sequence context on the function of cis-element and provide novel insights into the understanding of the mechanisms underlying the diversity of anthocyanin accumulation in grape berry.
Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Yibei Wang1,2, Junhua Kong1,2, Yongjian Wang1,2, Haiqi Wang1,2, Xiaobo Xu1,2, Boxing Shang1,2, Peige Fan1,2, Zhenchang Liang1,2, Zhanwu Dai1,2*

1 State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2 China National Botanical Garden, Beijing 100093, China

Contact the author*

Keywords

color, cis-regulation, sequence context, anthocyanin diversity, metabolism

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Improving stilbenes in vitis Labrusca L. Grapes through methyl jasmonate applications

Grapes (Vitis sp.) are considered a major source of phenolic compounds such as flavonols, anthocyanins and stilbenes. Studies related to the beneficial effects of these compounds on health have encouraged research aimed at increasing their concentration in fruits. On this behalf, several plant growth regulators such as jasmonic acid and its volatile ester, methyl-jasmonate (MeJa), have demonstrated promising results in many fruits. However, Brazilian subtropical climate might interfere on treatment response. The present study aims to evaluate the application of MeJa in the pre-harvest period in Concord and Isabel Precoce grapes (Vitis labrusca L.).

Read More

EXTRACTIBLE COMPOUNDS FROM MICROAGGLOMERATED CORK STOPPERS

After bottling, the wine continues to evolve during storage. The choice of the stopper is an important factor in this evolution. In addition to the oxygen permeability of the closure, the migration of stopper compounds into the wine can also have an impact on the wine organoleptic properties. Many studies have shown that transfers of volatile compounds from the stoppers into the wine can happen depending on the type of closure used (1). Moreover, when cork-made stoppers are used, the migration of phenolic compounds from the stopper into the wine can also occur (2, 3).

Read More

Wine tourism in southern Italy: A surge in popularity and economic impact

Wine tourism has transformed from a leisure activity into a crucial part of the tourist experience, significantly contributing to rural tourism’s expansion in italy. It has witnessed a notable surge in popularity in recent years, evolving as a key motivator for travel (antonioli corigliano, 2002; brunori & rossi, 2000; città del vino & censis servizi, 2011; garibaldi, 2018; 2019a; 2020; montanari, 2009; romano & natilli, 2009). The allure of wine tourism, driven by sensory experiences and cultural immersion, continues to attract a diverse group of tourists. The economic impact is substantial, with events and festivals contributing approximately €2.5 billion annually.

Read More

Pacific Northwest wine regions and climates

This paper presents a review of wine regions in the Pacific Northwest (PNW) of North America. The PNW consists of the states of Oregon, Washington and Idaho and the province of British Columbia.

Read More

Bio‐metaethics viticulture proposed by the Giesco. Direct charter with producers. Example of evaluation of training systems

The key points of the current GiESCO charter ‘BIO‐MetaEthics’ are exposed. The new development in cooperation with Giovanni Cargnello is to apply the principles and the content into the practice by establishing a direct contract with producers and other actors of the wine sector. An evaluation sheet is proposed and tested in a new advanced vineyard. For illustrating the methodology of evaluation, the example of the choice of the training systems is detailed on a wide range of situations. 

Read More