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IVES 9 IVES Conference Series 9 Autochthonous non-Saccharomyces extra-cellular metabolism of tryptophan, tyrosine, and phenylalanine

Autochthonous non-Saccharomyces extra-cellular metabolism of tryptophan, tyrosine, and phenylalanine


Amino acids are crucial nitrogen sources in yeast metabolism, influencing both biomass production and fermentation rate. The breakdown byproducts of amino acids contribute to the aroma of the wine and wine’s health benefit compounds. This study focused on the yeast’s extracellular metabolic profile of tryptophan, tyrosine, and phenylalanine belonging to the group of aromatic amino acids in experimental Maraština wines. Alcoholic fermentations were conducted on sterile grape Maraština must using seven autochthonous non-Saccharomycesyeasts in sequential fermentation with commercial Saccharomyces cerevisiae. Trials were performed with isolates Metschnikowia pulcherrima K-6, Metschnikowia chyrsoperlae K-11, Metschnikowia sinensis/shanxiensis P-7, Lachancea thermotolerans P-25, Pichia kluyveri Z-3, Hanseniaspora uvarum Z-7, and Hanseniaspora guillermondii N-29, each in triplicate. The control treatment involved commercial strains L. thermotolerans, M. pulcherrima, and S. cerevisiae. A UHPLC-QqQ-MS/MS method was employed to monitor 37 metabolites, with 26 detected in the extracellular extracts produced by yeasts. The most significant changes in the concentration of identified compounds occurred in M. sinensis/shanxiensis/S. cerevisiae and H. guillermondii/S. cerevisiae ferments. M. sinensis/shanxiensiswith S. cerevisiae produced higher amounts of N-acetyl derivatives of tryptophan and phenylalanine, as well as xanthurenic acid and tyramine. Wines produced by H. guillermondii in sequential fermentation with S. cerevisiae had the highest concentration of L-kynurenine and 3-hydroxy anthranilic acid. These findings contribute to our understanding of how autochthonous non-Saccharomyces yeasts contribute to the aroma profile of wines, providing new insights into biotechnological tools for the production of wine starter cultures.


Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster


Ana Boban1*, Urska Vrhovsek2, Andrea Anesi2, Vesna Milanović3, Irena Budić-Leto1

1 Institute for Adriatic Crops and Karst Reclamation, 21000 Split, Croatia
2 Metabolomics Unit, Research and Innovation Centre, Fondazione Edmund Mach, 38098 San Michele all’Adige, Italy
3 Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, Italy

Contact the author*


autochthonous yeast, wine, metabolism, non-Saccharomyces, starter culture


IVES Conference Series | Open GPB | Open GPB 2024


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