terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Abiotic interactions 9 From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

Abstract

One of the major challenges for food security and sovereignty is to produce stress-tolerant plants without introducing foreign DNA, because the legislative process, that bans transgenics, challenges us to find new solutions for producing plants that can survive the drought. To achieve this goal, we need to identify genes that can be modified to improve stress tolerance in plants. In this work, we present an online tool for exploring the transcriptome of grapevines under water stress, which is one of the most important abiotic stresses affecting viticulture. The tool is based on a comprehensive collection of rna-seq data from 997 experiments, covering four different tissues (leaf, root, berry, and shoot), various levels of water stress, and diverse genetic backgrounds (cultivars and rootstocks) with different levels of tolerance to water stress. The tool will be integrated within VitViz and Grapedia, two web portals that provide access to genomic data of grapevine. The tool allows us to compare the expression of all grapevine genes, using the V3 genome of ‘PN40024’ as a reference. With this app, we discovered a couple of genes that could boost the drought tolerance of grapevines by cis/trans-genesis (such as the raffinose synthase and AQUILO) or by genome editing (GRETCHEN HAGEN gene). We applied these two methods to different rootstocks and cultivars of grapevines.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2,Antonio Santiago Pajuelo2, Maria Stella Grando4, Felipe Gainza-Cortés3, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000 , Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38010 San Michele all’Adige, Italy

Contact the author*

Keywords

Transcriptome, Abiotic stress, Drought, Rna-seq, Bioinformatics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Influence of preflowering basal leaf removal on aromatic composition of cv. Tempranillo wine from semiarid climate (Extremadura Western Spain)

Abstract In this work the effects of early leaf removal performed manually at preflowering phenological stage, on the volatile composition of Tempranillo (Vitis vinifera L.) wines were studied. From 2009-2011 vintages 34 wine volatile compounds were identified and quantified by gas chromatography-mass spectrometry (GC-MS) where early leaf removal only modified 25 of them. The total C6 compounds, acetates and volatiles acids (with exception of isobutyric acid) were affected by defoliation, whereas alcohols and esters showed a minor effect. Furthermore the vintage effect also was shown.

Testing the effectiveness of Cell-Wall material from grape pomace as fining agent for red wines

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.

The impact of grazing by cattle on Vitis vinifera L. cv. Shiraz vegetative growth and metabolite profile

Context and purpose of the study. Globally, vineyard cultivation uses conventional methods to manage pests, diseases and increase yield.

L’étude “terroirs d’Anjou”: un exemple de caractérisation intégrée des terroirs viticoles, utilisable à l’échelle parcellaire

Natural factors of the production (“terroir” and vintage) are known as an important element for identifying wines by their genuine typicité and their authenticity. The program “Terroirs d’Anjou” (1994-1999) aims at bringing the necessary scientific basis for a rational and reasoned exploitation of the terroir.

Is complex nutrition more advantageous than mineral nitrogen for the fermentative capacities of S. cerevisiae?

During alcoholic fermentation, nitrogen is an essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (yan) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species which may lead to economic losses. However, correcting this nitrogen deficiency is sometimes not enough to restore proper fermentation performance. This suggests the existence of other nutritional shortages.