terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Abiotic interactions 9 From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

Abstract

One of the major challenges for food security and sovereignty is to produce stress-tolerant plants without introducing foreign DNA, because the legislative process, that bans transgenics, challenges us to find new solutions for producing plants that can survive the drought. To achieve this goal, we need to identify genes that can be modified to improve stress tolerance in plants. In this work, we present an online tool for exploring the transcriptome of grapevines under water stress, which is one of the most important abiotic stresses affecting viticulture. The tool is based on a comprehensive collection of rna-seq data from 997 experiments, covering four different tissues (leaf, root, berry, and shoot), various levels of water stress, and diverse genetic backgrounds (cultivars and rootstocks) with different levels of tolerance to water stress. The tool will be integrated within VitViz and Grapedia, two web portals that provide access to genomic data of grapevine. The tool allows us to compare the expression of all grapevine genes, using the V3 genome of ‘PN40024’ as a reference. With this app, we discovered a couple of genes that could boost the drought tolerance of grapevines by cis/trans-genesis (such as the raffinose synthase and AQUILO) or by genome editing (GRETCHEN HAGEN gene). We applied these two methods to different rootstocks and cultivars of grapevines.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2,Antonio Santiago Pajuelo2, Maria Stella Grando4, Felipe Gainza-Cortés3, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000 , Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38010 San Michele all’Adige, Italy

Contact the author*

Keywords

Transcriptome, Abiotic stress, Drought, Rna-seq, Bioinformatics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Effects of Non-Grape Materials (MOG) on wine quercetin composition: insights from synthetic and Merlot grape juice fermentation

Quercetin precipitation has become an increasingly common issue in red wine, often resulting in visually unpleasant sediments and diminished product quality.

Bench terraces, agricultural practices and viticultural zoning in Ribeira Sacra (Galicia, Spain).

L’aire d’AOC Ribeira Sacra s’étend sur plus de 200 km au large des versants escarpés du Miño et du Sil, dans la Galice (Espagne).

Portable NIR spectroscopy for nutrient profiling in rootstock and scion material: enhancing decision-making in the grafting industry

The success of grafting in viticulture is deeply influenced by the nutrient composition of both rootstock and scion
materials. Key components such as nitrogen and carbohydrates play a crucial role in graft compatibility, establishment,
and overall plant vigor [1].

Caractérisation du terroir en Espagne : méthodologie de l’évaluation et de la validation

In recent years, there has been a growing interest in characterizing the ecological environment of vineyard production, and the growing need to delimit and characterize with precision the different homogeneous viticultural units. This has allowed the development of new studies which have as their objective the Vineyard Zoning. The delimitation and characterization of wine-growing areas poses specific problems in Spain, not only linked to the specific characteristics of the territory, but also to the size, distribution and index of viticultural occupation in the designations of origin.

The challenge of improving oenological quality in favorable conditions for productivity

Marselan (Cabernet-Sauvignon x Grenache), has been planted for more than 20 years now in Uruguay. Due to its good agronomic and oenological aptitudes under uruguayan conditions, it is currently the red variety with highest plantation rate. The objective of the study was to identify management practices, aimed at improving quality in highly productive vineyards, different fruit/leaf regulation methods were tested in southern Uruguay.