terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Abiotic interactions 9 From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

Abstract

One of the major challenges for food security and sovereignty is to produce stress-tolerant plants without introducing foreign DNA, because the legislative process, that bans transgenics, challenges us to find new solutions for producing plants that can survive the drought. To achieve this goal, we need to identify genes that can be modified to improve stress tolerance in plants. In this work, we present an online tool for exploring the transcriptome of grapevines under water stress, which is one of the most important abiotic stresses affecting viticulture. The tool is based on a comprehensive collection of rna-seq data from 997 experiments, covering four different tissues (leaf, root, berry, and shoot), various levels of water stress, and diverse genetic backgrounds (cultivars and rootstocks) with different levels of tolerance to water stress. The tool will be integrated within VitViz and Grapedia, two web portals that provide access to genomic data of grapevine. The tool allows us to compare the expression of all grapevine genes, using the V3 genome of ‘PN40024’ as a reference. With this app, we discovered a couple of genes that could boost the drought tolerance of grapevines by cis/trans-genesis (such as the raffinose synthase and AQUILO) or by genome editing (GRETCHEN HAGEN gene). We applied these two methods to different rootstocks and cultivars of grapevines.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2,Antonio Santiago Pajuelo2, Maria Stella Grando4, Felipe Gainza-Cortés3, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000 , Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38010 San Michele all’Adige, Italy

Contact the author*

Keywords

Transcriptome, Abiotic stress, Drought, Rna-seq, Bioinformatics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sensory evaluation of grape berries: predictive power for sensory properties of Sauvignon blanc, Riesling and Pinot noir wines

Sensory analysis of grape berries is a common tool to evaluate the degree of grape maturation and to make sound picking decisions.

Spotted lanternfly, a new invasive insect in vineyards: is it a threat to grapevines?

The spotted lanternfly (SLF; Lycorma delicatula) is a phloem-feeding polyphagous insect invasive to the Eastern U.S.. Since its first detection in Pennsylvania (U.S.) in 2014, large infestations and economic damage (e.g., decreased yield, vine decline, greater pesticide use) have been reported in an increasing number of vineyards, threatening the sustainability and growth of the wine industry in infested regions. Our team has been investigating the impacts of SLF phloem-feeding on physiological processes, fruit production, juice, and wine composition of different grape cultivars, and also evaluated if the SLF can transmit important grapevine pathogens. In addition, we are working closely with stakeholders to better enumerate the economic damage caused by this pest. These findings will provide relevant information to grape and wine producers to help identify action thresholds and develop a more targeted integrated pest management program.

Impact of fining agents on Swiss Pinot noir red wines

In the context of climate change, excessive bitterness and astringency in wines have become increasingly prevalent. While variety selection and viticultural practices offer long-term solutions, they require considerable time before yielding practical results. In contrast, fining remains an accessible and immediate tool for winemakers.

Effect of ozone treatments in wine production on colour traits, volatile composition, and sensory characteristics of young and short-term aged white wines

The main aim of WiSSaTech project (PRIN P2022LXY3A),supported by the Italian Ministero dell’Università e della Ricerca and European Union-NextGenerationEU, is to investigate eco-friendly and safe alternatives to sulphur dioxide (SO2) in wine production.

Terroir et variabilité microclimatique : pour une approche à l’échelle de la parcelle

The climatic component is one of the elements of the zoning of viticultural potential, alongside the geological and pedological components (Morlat, 1989; Lebon et al , 1993). Many climatic indices have thus been defined to estimate the potential for wine production at the scale of a region or a country (Carbonneau et al ., 1992). The main climatic variables used are temperature and radiation. We note in particular the indices of Branas, Huglin and Ribereau-Gayon (Huglin, 1986). However, few studies have been undertaken on the spatial variability of microclimatic conditions at the scale of a vineyard, a valley, or even a municipality.