From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

The effects of different oxygen and sulphur dioxide additions to South African ‘Sauvignon blanc’ musts were investigated. Oxygen addition without SO2 protection led to lower levels of certain volatile thiols in the wines, with a corresponding decrease in certain phenols and glutathione concentrations.

After Champagne popping, the first consumer’s observation is the shape of the cork stopper. Consumers expect a “mushroom shape”. Nevertheless, we sometimes observe a “barrel” shape due to inappropriate cork’s elastic properties. The aim of this study was to follow the loss of cork stopper resiliency during 26 months according to the density (d) of the cork in contact with the wine. 1680 disks were weighed + measured and divided in 6 density classes: High (H1 d= 0,19 g/cm3 – H2 d= 0,21 g/cm3), Medium (M, not studied) and Low (L1 d= 0,13 g/cm3 – L2 d= 0,14 g/cm3). Then, 138 technical cork stoppers were produced for each of the 4 studied groups. These corks consisted of an agglomerated natural cork granule body to which two natural cork disks were glued. A total of 552 bottles of sparkling wine were closed with these corks and open after 13, 19 and 26 months to follow cork resiliencies. Wine bottles were stored horizontally; thus, the external natural cork disks were in contact to the wine. During the 26 months of the study, highly significant differences (ANOVA) were observed between the resiliencies of H-corks and those of L-corks, whatever the time studied. The diameters of the L-corks were statistically higher than those of the H-corks. No significant differences were observed between L1 and L2 corks. At the opposite, differences were noted between H1 and H2 at 19 and 26 months. This could be explained by the heterogeneity of the resiliency that was higher for H-corks than for L-corks. Finally, the corks were visually (12 judges) divided in 3 classes corresponding to high (expected mushroom shape, i.e high resiliency), medium (irregular shape of the disk in contact with the wine and/or low premature deterioration of the expected resiliency) and low qualities (barrel shape = premature deterioration of the resiliency). The corks were also divided in 3 categories corresponding to 0-33%, 34-66% and 67-100% resiliency. A strong correlation was noted between the visual and the instrumental categorizations. This study strongly evidenced 1) the importance of the cork density on the cork stopper behaviour when opening the bottle and 2) the interest of an instrumental approach reflecting the consumer’s perception.

La brise de mer et les facteurs climatiques qu’elle entraîne (accélération de la vitesse du vent au cours de l’après midi, augmentation de l’humidité et baisse de la temperature) sont d’un intérêt particulier pour la viticulture.

The study of wine evolution during barrel aging is an important aspect of wine quality. Our previous works have shown that wine metabolome monitoring by

1H-NMR approaches allows determining the impact of different winemaking processes including traitements using enzymes or finning agents [1].

In response to changes in their environment, grapevines regulate transpiration using various physiological mechanisms that alter conductance of water through the soil-plant-atmosphere continuum. Expressed as bulk stomatal conductance at the canopy scale, it varies diurnally in response to changes in vapor pressure deficit and net radiation, and over the season to changes in soil water deficits and hydraulic conductivity of both soil and plant. It is necessary to characterize the response of conductance to these variables to better model how vine transpiration also responds to these variables. Furthermore, to be relevant for vineyard-scale modeling, conductance is best characterized using data collected in a vineyard setting. Applying a crop canopy energy flux model developed by Shuttleworth and Wallace, bulk stomatal conductance was estimated using measurements of individual vine sap flow, temperature and humidity within the vine canopy, and estimates of net radiation absorbed by the vine canopy. These measurements were taken on several vines in a non-irrigated vineyard in Bordeaux France, using equipment that did not interfere with ongoing vineyard operations. An inverted Penman-Monteith equation was then used to calculate bulk stomatal conductance on 15-minute intervals from July to mid-September 2020. Time-series plots show significant diurnal variation and seasonal decreases in conductance, with overall values similar to those in the literature. Global sensitivity analysis using non-parametric regression found transpiration flux and vapor pressure deficit to be the most important input variables to the calculation of bulk stomatal conductance, with absorbed net radiation and bulk boundary layer conductance being much less important. Conversely, bulk stomatal conductance was one of the most important inputs when calculating vine transpiration, further emphasizing the need for characterizing its response to environmental changes for use in vineyard water use modeling.