terclim by ICS banner
IVES 9 IVES Conference Series 9 Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Abstract

Water resource is a major limiting factor impacted by climate change that threatens grapevine production and quality. Understanding the ecophysiological mechanisms involved in the response to water deficit is crucial to select new varieties more drought tolerant. A major bottleneck that hampers such advances is the lack of methods for measuring fine functioning traits on thousands of plants as required for genetic analyses. This study aimed at investigating how water deficit affects the trade-off between carbon gains and water losses in a large panel representative of the Vitis vinifera genetic diversity. 250 genotypes were grown under 3 watering scenarios (well-watered, moderate and severe water deficit) in a high-throughput phenotyping platform. To assess traits related to carbon and water functioning on the whole panel, we deployed an original approach where 120 leaves of 40 genotypes were phenotyped combining low-throughput devices to precisely measure ecophysiological traits, as well as innovative, portable high-throughput devices to measure near infrared reflectance, porometry and chlorophyll fluorescence. These data allowed us to build cutting-edge statistical models, such as multiblock models, which jointly use data from different devices, for predicting ecophysiological traits. Models for predicting photosynthesis and transpiration were accurate enough to be applied on the entire panel, only measured with high-throughput devices. Such predictions highlighted a wide range of genotypic variability and contrasting responses to water deficit. Multi-traits and Multi-Environment Genome Wide Association Studies further revealed genomic regions associated with these responses, and underlying candidate genes are being investigated. 

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Eva Coindre1,2*, Laurine Chir2, Maxime Ryckewaert3, Romain Boulord2, Mélyne Falcon2, Thomas Laisné2, Gaëlle Rolland2, Maëlle Lis2, Llorenç Cabrera-Bosquet2, Agnès Doligez1, Thierry Simonneau2, Benoît Pallas2, Aude Coupel-Ledru2, Vincent Segura1,4

1 AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France
2 LEPSE, Univ Montpellier, INRAE, Institut Agro, Montpellier, France
3 Inria, LIRMM, Univ Montpellier, CNRS, Montpellier, France
4 UMT Geno-Vigne, IFV, INRAE, Montpellier, France

Contact the author*

Keywords

water deficit, high throughput phenotyping, prediction, photosynthesis/transpiration coupling, GWAS

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock.

Read More

The future of pesticide regulation in the EU – between precaution and proportionality

The article analyzes current developments in European pesticide regulation.

Read More

Effects of environmental factors and vineyard pratices on wine flora dynamics

he intensification of t vineyard practices led to an impoverishment of the biological diversity. In vineyard management, the reflection to reduce pesticides uses concerns mainly the soil management of the vineyard, and often focuses on flora management in the inter-row.

Read More

Development of bioprospecting tools for oenological applications

Wine is the result of a complex biochemical process. From a microbiological point of view, the grape berry is characterised by a heterogeneous microbiota composed of different microorganisms (yeasts, bacteria and filamentous fungi) which will play a predominant role in the quality of the final product. At this level, yeasts play a predominant role in the chemistry of wine, as they

Read More

FERMENTATION POTENTIAL OF INDIGENOUS NON-SACCHAROMYCES YEASTS ISOLATED FROM MARAŠTINA GRAPES OF CROATIAN VINEYARDS

The interest in indigenous non-Saccharomyces yeast for use in wine production has increased in recent years because they contribute to the complex character of the wine. The aim of this work was to investigate the fermentation products of ten indigenous strains selected from a collection of native yeasts established at the Institute for Adriatic Crops and Karst Reclamation in 2021, previously isolated from Croatian Maraština grapes, belonging to Hypopichia pseudoburtonii, Metschnikowia pulcherrima, Metschnikowia sinensis, Metschnikowia chrysoperlae, Lachancea thermotolerans, Pichia kluyveri, Hanseniaspora uvarum, Hanseniaspora guillermondii, Hanseniaspora pseudoguillermondii, and Starmerella apicola species, and compare it with commercial non-Saccharomyces and Saccharomyces strains.

Read More