terclim by ICS banner
IVES 9 IVES Conference Series 9 Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Abstract

Water resource is a major limiting factor impacted by climate change that threatens grapevine production and quality. Understanding the ecophysiological mechanisms involved in the response to water deficit is crucial to select new varieties more drought tolerant. A major bottleneck that hampers such advances is the lack of methods for measuring fine functioning traits on thousands of plants as required for genetic analyses. This study aimed at investigating how water deficit affects the trade-off between carbon gains and water losses in a large panel representative of the Vitis vinifera genetic diversity. 250 genotypes were grown under 3 watering scenarios (well-watered, moderate and severe water deficit) in a high-throughput phenotyping platform. To assess traits related to carbon and water functioning on the whole panel, we deployed an original approach where 120 leaves of 40 genotypes were phenotyped combining low-throughput devices to precisely measure ecophysiological traits, as well as innovative, portable high-throughput devices to measure near infrared reflectance, porometry and chlorophyll fluorescence. These data allowed us to build cutting-edge statistical models, such as multiblock models, which jointly use data from different devices, for predicting ecophysiological traits. Models for predicting photosynthesis and transpiration were accurate enough to be applied on the entire panel, only measured with high-throughput devices. Such predictions highlighted a wide range of genotypic variability and contrasting responses to water deficit. Multi-traits and Multi-Environment Genome Wide Association Studies further revealed genomic regions associated with these responses, and underlying candidate genes are being investigated. 

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Eva Coindre1,2*, Laurine Chir2, Maxime Ryckewaert3, Romain Boulord2, Mélyne Falcon2, Thomas Laisné2, Gaëlle Rolland2, Maëlle Lis2, Llorenç Cabrera-Bosquet2, Agnès Doligez1, Thierry Simonneau2, Benoît Pallas2, Aude Coupel-Ledru2, Vincent Segura1,4

1 AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France
2 LEPSE, Univ Montpellier, INRAE, Institut Agro, Montpellier, France
3 Inria, LIRMM, Univ Montpellier, CNRS, Montpellier, France
4 UMT Geno-Vigne, IFV, INRAE, Montpellier, France

Contact the author*

Keywords

water deficit, high throughput phenotyping, prediction, photosynthesis/transpiration coupling, GWAS

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Copper reduction strategy for sangiovese in organic viticulture

Organic viticulture requires copper based treatments for bunch protection even though an intensive employment is no longer admitted because of its low leaching and phytotoxicity in the soil. UE Reg. 1981/2018 set copper employment to 4 kg/ha for year or 28 during 7 years with an absolute level allowed of 6 Kg/ha although those limits were decreased frequently.

Read More

Hanseniaspora in wine-making: their genetic modification and potential role in acid modulation

Hanseniaspora spp. are one of the most common yeast isolates in vineyards and wineries and play an important role in wine-making.

Read More

Illuminating vineyard management: Elevating operational efficiency through advanced sensing and data analytics

In this video recording of the IVES science meeting 2024, Luca Brillante (California State University, Fresno, USA) speaks about vineyard management, advanced sensing and data analytics. This presentation is based on an original article accessible for free on OENO One.

Read More

Chemical profiling and sensory analysis of wines from resistant hybrid grape cultivars vs conventional wines

Recently, there has been a shift toward sustainable wine production, according to EU policy (F2F and Green Deal), to reduce pesticide usage, improve workplace health and safety, and prevent the impacts of climate change. These trends have gained the interest of consumers and winemakers. The cultivation of disease resistant hybrid grape cultivars (DRHGC), known as ‘PIWI’ grapes can help with these objectives [1]. This study aimed to profile white and red wines produced from DRHGC in South Tyrol (Italy). Wines produced from DRHGCs were compared with conventional wines produced by the same wineries. The measured parameters were residual sugars, organic acids, alcohol content, pigments and other phenolics by LC-QqQ/MS, colorimetric indexes (CIELab); and volatile profiles (HS-SPME-GCxGC-ToF/MS [2]).

Read More

Evaluation of aroma characteristics in Vitis amurensis grapes across different regions by using HS-SPME-GC/MS

Background: Aroma compounds are important secondary metabolite in grapes and play important roles in the flavor and quality of grape berries and their wines. Vitis amurensis grape belongs to the East Asian Vitis spp., with excellent cold and disease resistance, and exhibits strong brewing potential. However, it has not been effectively utilized and there is no systematic research on the aroma compounds of V. amurensis grapes.
Methods: To provide sufficient experimental evidence for the characteristic aroma of V. amurensis grape, HS-SPME-GC/MS was used to identify the aroma compounds of five V. amurensis (‘Beiguohong’, ‘Beiguolan’, ‘Shuangfeng’, ‘Shuanghong’, ‘Shuangyou’) and three interspecific hybrids (‘Beibinghong’, ‘Xuelanhong’, ‘Zuoyouhong’) grapes in Zuojia and Ji’an. The grape berries were collected at harvest in 2020, 2021 and 2022.

Read More