terclim by ICS banner
IVES 9 IVES Conference Series 9 Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Abstract

Water resource is a major limiting factor impacted by climate change that threatens grapevine production and quality. Understanding the ecophysiological mechanisms involved in the response to water deficit is crucial to select new varieties more drought tolerant. A major bottleneck that hampers such advances is the lack of methods for measuring fine functioning traits on thousands of plants as required for genetic analyses. This study aimed at investigating how water deficit affects the trade-off between carbon gains and water losses in a large panel representative of the Vitis vinifera genetic diversity. 250 genotypes were grown under 3 watering scenarios (well-watered, moderate and severe water deficit) in a high-throughput phenotyping platform. To assess traits related to carbon and water functioning on the whole panel, we deployed an original approach where 120 leaves of 40 genotypes were phenotyped combining low-throughput devices to precisely measure ecophysiological traits, as well as innovative, portable high-throughput devices to measure near infrared reflectance, porometry and chlorophyll fluorescence. These data allowed us to build cutting-edge statistical models, such as multiblock models, which jointly use data from different devices, for predicting ecophysiological traits. Models for predicting photosynthesis and transpiration were accurate enough to be applied on the entire panel, only measured with high-throughput devices. Such predictions highlighted a wide range of genotypic variability and contrasting responses to water deficit. Multi-traits and Multi-Environment Genome Wide Association Studies further revealed genomic regions associated with these responses, and underlying candidate genes are being investigated. 

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Eva Coindre1,2*, Laurine Chir2, Maxime Ryckewaert3, Romain Boulord2, Mélyne Falcon2, Thomas Laisné2, Gaëlle Rolland2, Maëlle Lis2, Llorenç Cabrera-Bosquet2, Agnès Doligez1, Thierry Simonneau2, Benoît Pallas2, Aude Coupel-Ledru2, Vincent Segura1,4

1 AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, France
2 LEPSE, Univ Montpellier, INRAE, Institut Agro, Montpellier, France
3 Inria, LIRMM, Univ Montpellier, CNRS, Montpellier, France
4 UMT Geno-Vigne, IFV, INRAE, Montpellier, France

Contact the author*

Keywords

water deficit, high throughput phenotyping, prediction, photosynthesis/transpiration coupling, GWAS

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

CHARACTERIZATION OF THE VOLATILE COMPOUNDS PROFILE OF COMMERCIAL GRAPPAS OBTAINED FROM THE POMACE OF AMARONE WINES

Grappa is a traditional Italian alcoholic beverage, with an alcohol content generally between 40-60% vol., obtained from the distillation of grape pomace used for the production of wine. Grappa are often aged in wooden barrels. There are various types of grappa: young, aromatic, aged, extra-aged depending on whether the distillate comes from aromatic vines or is aged in wooden barrels for shorter or longer periods. There is also flavored grappa if herbs, fruit or roots are added. All this makes it an extremely heterogeneous product both from an organoleptic and compositional point of view.

Effects of regulated deficit irrigation (RDI) on grape composition in Monastrell grapevines under semiarid conditions

The influence of two pre-veraison and post-veraison regulated deficit irrigation (RDI) strategies on yield and grape quality was analyzed during a two year period for mature grapevines (cv. Monastrell) in Southeastern of Spain

Direct-injection HPLC for simultaneous determination of individual and total polyphenols in red wines: validation of the method

Polyphenols are very important compounds of red wines, serving as essential bioactive components and playing an important role in sensory properties. The determination of individual phenolic compounds in red wine is commonly performed by HPLC analysis, while the total polyphenols are quantified by spectrophotometric methods, usually by the method of absorbance at 280 nm (index of ribéreau-gayon) or the method of index of folin-ciocalteu. In this work, we pioneeringly proposed a new and fast method for simultaneous determination of individual and total polyphenols in red wines by direct-injection HPLC without sample preparation.

Microbial consortia as a tool for sustainable vineyard management: A study on their acceptance among Veneto region’s grape-growers

In recent years, sustainability has become a key focus in agriculture, including viticulture.