terclim by ICS banner
IVES 9 IVES Conference Series 9 Integrated approaches for the functional characterization of miRNAs in grapevine

Integrated approaches for the functional characterization of miRNAs in grapevine

Abstract

Micro(mi)RNAs are small non-coding RNAs that regulate several pathways and are widely recognised as key players in plant development, tissue differentiation, and many other important physiological processes, including plant adaptation to biotic and abiotic stresses. The release of plant genomes and the application of high throughput sequencing have considerably extended miRNA discovery across many species, including grapevine (Vitis spp.). Despite their relevance in plant development, functional studies in grapevine to clarify the function of miRNAs are not yet available. Through the grapevine genetic improvement platform IMPROVIT at CNR-IPSP (http://www.ipsp.cnr.it/en/thematics/turin-headquarter-thematics/improvit/), we developed integrated approaches to discover miRNA function in grapevine. Transgenic plants were generated by either overexpressing or silencing vvi-miR393, a miRNA conserved in different plant species, and vvi_miC137, a grapevine-specific miRNA whose function is unknown despite being transcriptionally regulated in response to biotic and abiotic stresses. A total of 212 transgenic grapevines from two V. vinifera cultivars (Chardonnay and Bragat rosa) and the 110R rootstock were characterized. Molecular analyses showed that overexpressing lines increased the expression of the selected miRNAs up to 10-fold, whereas silencing by short tandem target mimic (STTM) approach reduced the expression of mature miRNAs by 70%. Five independed transgenic lines for each genotype and construct were acclimatised in greenhouse. Additionally, vvi_miC137 lines were ecophysiologically characterized under well-watered and drought conditions. Preliminary results showed that vvi_miC137 influenced plant development and leaf gas exchanges, its partial silencing improved grapevine growth performance. This miRNA could represent a new potential target for genetic improvement by gene editing.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Chiara Pagliarani1, Amedeo Moine1, Anastasiia Kasianova1,2, Paolo Boccacci1, Luca Nerva3, Andrea Delliri1, Claudio Lovisolo2, Walter Chitarra3, Irene Perrone1, Giorgio Gambino1*

1 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
2 Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco (TO), Italy
3 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)

Contact the author*

Keywords

miRNAs, genetic transformation, functional studies, grapevine development, water stress

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Multisensory experiential wine marketing

Interest in the pairing, or matching, of wine with music goes way back, with commentators initially using musical metaphors merely to describe the wines that they were writing about. More recently, however, this has transformed into a growing range of multisensory tasting events in which wine and music are deliberately paired to assess, or increasingly to illustrate, the impact of the latter on

Modulation of berry composition by different vineyard management practices

High concentration of sugars in grapes and alcohol in wines is one of the consequences of climate change on viticulture production in several wine-growing regions. In order to investigate the possibilities of adaptation of vineyard management practices aimed to reduce the accumulation of sugar during the maturation phase without reducing the accumulation of anthocyanins in grapes, a study with severe shoot trimming, shoot thinning, cluster thinning and date of harvest was conducted on Merlot variety in Istria region (Croatia), under the Mediterranean climate. Four factors which may affect grape maturation and its composition at harvest were investigated in a two-years experiment; severe shoot trimming applied at veraison when >80% of berries changed colour (in comparison to untreated control), shoot thinning (0 and 30%), cluster thinning (0 and 30%), and the date of harvest (early and standard harvest dates). Shoot thinning had no significant impact on berry composition, despite the obtained reduction in yield per vine. Lower Brix in grapes were obtained with earlier harvest date and if no cluster thinning was applied, although at the same time a reduction in the concentration of anthocyanins in berries was observed in these treatments. On the other hand, if severe shoot trimming was applied when >80% of berries changed colour, a reduction of Brix was obtained without a negative impact on berry anthocyanins concentration. We conclude that in cases when undesirably high sugar concentrations at harvest are expected, severe shoot trimming at 80% veraison may effectively be used in order to obtain moderate sugar concentration in berries together with the adequate phenolic composition.

Contribution of seeds to red wine phenolic composition

Tannin composition is an important attribute in red wine quality, and it is therefore critical to understand the factors influencing tannin extraction during alcoholic fermentation. Tannins contribute to the mouthfeel of wines, but they also form pigmented polymers...

Il Lambrusco reggiano e il territorio di pianura: un modello efficace

Il caso “Lambrusco” è emblematico di un buon connubio tra un gruppo di vitigni ed un territorio di pianura caratterizzato da suoli fertili e alluvionali, che determinano un elevato sviluppo

Study of fungal and bacterial laccases for the reduction of ochratoxin A content in model wine

Ochratoxin A (OTA) is a mycotoxin produced by several filamentous fungi infecting grape bunches (Penicillium and Aspergillus spp.), this toxin pass to must when grapes are crushed and later it is found in wine. Following the evaluations of the toxicity of OTA, European Commission Regulations have been promulgated introducing upper limits for OTA concentrations in various commodities (cereals, cereal products, dried vine fruit, coffee, wine, grape juice, baby foods and dietary foods for special medical purposes).