terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Abstract

Global warming has enhanced the frequency and severity of drought events, hence calling for a better management of water resources in the vineyard and for an improvement of breeding platforms. Somatic embryogenesis (SE) (i.e. the initiation of embryos from somatic tissues) can spontaneously generate new genetic variability, which results from genetic mutations, changes in epigenetic marks, or phenotypic alterations.

This study was tailored to test whether vines in vitro regenerated through SE (i.e. somaclones), can tolerate water deprivation better than the mother plant.

Physiological trials of water stress and recovery were conducted on two populations of different somaclone lines of Vitis vinifera ‘Nebbiolo’ and of 110R (V. rupestris x V. berlandieri) rootstock, respectively regenerated in absence and presence of in vitro selective pressure. During the experiments, dynamic changes in the main eco-physiological parameters were monitored on target somaclones and compared with those measured on plants of the corresponding mother plant lines. Alterations in biometric and anatomical traits were also inspected. The observed responses were further deepened by analyzing differences in the accumulation of defense secondary metabolites and hormones and in the transcription of stress-responsive genes. In parallel, genetic mutations potentially controlling specific physiological adjustments were searched, by sequencing the genomes of the best and worst performing lines.

The integration of physiological, biochemical and molecular data proved that grapevine somaclones are more tolerant to drought and that therefore the exploitation of somaclonal variability can represent an effective and ready-to-use genetic improvement strategy for implementing clonal selection and breeding programs in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Amedeo Moine1*, Paolo Boccacci1, Walter Chitarra1,2, Luca Nerva1,2, Giorgio Gambino1, Irene Perrone1, Chiara Pagliarani1

1 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)

Contact the author*

Keywords

Vitis Spp., water stress, somatic embryogenesis, genetic variability, gas exchange

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Great highlands wine growing terroir: conditions and expressions

During 1982 started our wine growing project at the Puntalarga Hill, between 2500 and 2600 meters a.s.l.: 5.78 ºN, 72.98 ºW. Pinot noir, white Riesling and Riesling x Silvaner crossings are the most planted grapevines. Since 1984 research and development activities are carried out on pertinent subjects.

Revisiting the effect of subsurface irrigation and partial rootzone drying on canopy size and yield of Cabernet Sauvignon using remote sensing techniques

Irrigation is an essential tool for grape production, especially where rainfall does not meet the optimal water requirements needed to achieve yield and quality targets. Increased evaporative demand of grapevines due to changing climate conditions, and a growing awareness for sustainable farming, require the improvement of irrigation techniques to maximize water use efficiency, i.e. using less water to achieve the same yields or the same water but larger yields. In this study, the performance of Cabernet Sauvignon vines was compared under three irrigation techniques: conventional aboveground drip irrigation, subsurface irrigation installed directly under the vine row, and partial rootzone drying in which two subsurface lines were buried in the middle of the two interrow spacings on each side of the vine row with irrigation alternated between the two lines based on soil moisture content.

Late winter pruning induces a maturity delay under temperature-increased conditions in cv. Merlot from Chile

Chile is considered vulnerable to climate change; and these phenomena affect several mechanisms in the grape physiology and quality. The global temperature increase affects sugar contents, organic acids, and phenolic compounds in grapes, producing an imbalance maturity. In this sense, an alternative to reduce the impact is to perform pruning after vine budburst, known as “Late Pruning” (LP).

Analytical characterization of Oloroso Sherry in Sherry Cask seasoning and its influence in the ageing of brandy de jerez

Oloroso Sherry is a typical fortified wine from Jerez de la Frontera (south of Spain). It is one of the most used in the seasoning of oak barrels, called Sherry Cask, destined in this area for ageing brandies or condiments as wine vinegars. Brandy de Jerez is an European Geographical Indication for grape-derived spirits. Its special organoleptic characteristics are due to its traditional dynamic ageing in Sherry Casks. American oak is the most common wood employed in Jerez area, where Brandy de Jerez is exclusively manufactured. During ageing period of Sherry and brandies, the wood is not only a container, it is involved in several physicochemical process with the Sherry or the distillate. Oak wood is the responsible of the presence of many compounds in the products, affecting their aroma and chemical composition and having a high influence in their final quality. Moreover, the seasoned wood with Sherry wine could transfer the compounds from wine into the brandy, improving its aroma and flavor.

Towards an ecological architecture inspired by underground cellars: An example of the thermal inertia of Moldovan underground cellars and new geothermal and Canadian well approaches

The search for underground shelters is one of the oldest forms of human habitation, providing refuge in extreme environments such as deserts and polar regions.