terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Abstract

Global warming has enhanced the frequency and severity of drought events, hence calling for a better management of water resources in the vineyard and for an improvement of breeding platforms. Somatic embryogenesis (SE) (i.e. the initiation of embryos from somatic tissues) can spontaneously generate new genetic variability, which results from genetic mutations, changes in epigenetic marks, or phenotypic alterations.

This study was tailored to test whether vines in vitro regenerated through SE (i.e. somaclones), can tolerate water deprivation better than the mother plant.

Physiological trials of water stress and recovery were conducted on two populations of different somaclone lines of Vitis vinifera ‘Nebbiolo’ and of 110R (V. rupestris x V. berlandieri) rootstock, respectively regenerated in absence and presence of in vitro selective pressure. During the experiments, dynamic changes in the main eco-physiological parameters were monitored on target somaclones and compared with those measured on plants of the corresponding mother plant lines. Alterations in biometric and anatomical traits were also inspected. The observed responses were further deepened by analyzing differences in the accumulation of defense secondary metabolites and hormones and in the transcription of stress-responsive genes. In parallel, genetic mutations potentially controlling specific physiological adjustments were searched, by sequencing the genomes of the best and worst performing lines.

The integration of physiological, biochemical and molecular data proved that grapevine somaclones are more tolerant to drought and that therefore the exploitation of somaclonal variability can represent an effective and ready-to-use genetic improvement strategy for implementing clonal selection and breeding programs in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Amedeo Moine1*, Paolo Boccacci1, Walter Chitarra1,2, Luca Nerva1,2, Giorgio Gambino1, Irene Perrone1, Chiara Pagliarani1

1 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)

Contact the author*

Keywords

Vitis Spp., water stress, somatic embryogenesis, genetic variability, gas exchange

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Ampelograpic and genetic characterisation of grapevine genetic resources from Ozalj-Vivodina region (Croatia)

Ozalj- vivodina region is small vine growing area (only about 100 hectares of vineyards), but with significant number of old, ancient vineyards planted between 50 and 100 years ago. Trend of abandoning or replanting ancient vineyards takes place for the last 30 years. This trend results in grapevine germplasm erosion because traditional varieties are replaced with well known international varieties.Few known traditional varieties are dominantly present in ancient vineyards together with many others of unknown identity. Historical data about prevalence and characteristic of varieties on this area are very poor.

Territorial delimitation of viticultural “Oltrepo Pavese (Lombardy)” using grape ripening precocity

L’Oltrepò Pavese est une zone de collines de la Lombardie, région située au nord de l’Italie avec un vignoble qui s’étend sur près de 15 000 ha. Cette zone représente la plus grande aire de production de la région et une des A.O.C. les plus étendues de tout le pays. Les cépages les plus cultivés, même historiquement, sont autochtones : la Barbera et la Croatina utilisés pour la production de vin rouge «Oltrepò» et le Pinot noir pour la production de vins mousseux. Pour le zonage viticole de cette A.O.C., il a été pris en considération: le climat, les sols, les caractéristiques viti-vinicoles.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

Influence of the different cork stoppers and sulfur dose in champagne quality

As is well known, Champagne is a product of the highest quality recognized in the international market. Champagne is a type of sparkling wine made in the Champagne region

Effect of regulated deficit irrigation regime on amino acids content of Monastrell (Vitis vinifera L.) grapes

Irrigation is an important practice to influence vine quality, especially in Mediterranean regions, characterized by hot summers and severe droughts during the growing season. This study focused on deficit irrigation regime influence on amino acids composition of Monastrell grapevines under semiarid conditions (Albacete, Southeastern of Spain). In 2019, two treatments were applied: non-irrigation (NI) and regulated deficit irrigation (RDI), watered at 30% of the estimated crop evapotranspiration from fruit set to onset of veraison. Grape amino acids content was analyzed by HPLC. Berries from non-irrigated vines showed higher concentration of several amino acids, such as tryptophan (73%), arginine (70%), lysine (36%), isoleucine (27%), and leucine (21%), compared to RDI grapes. Arginine is, together with ammonium ion, the principal nitrogen source for yeasts during the alcoholic fermentation; while isoleucine, tryptophan, and leucine are precursors of fermentative volatile compounds, key compounds for wine quality. Moreover, NI treatment increased in a 14% the total amino acids content in grapes compared to RDI treatment. The reported effects might be because yield was 70% higher in RDI vines than in the NI ones and, therefore, the sink demand was increased in the irrigated vines. In addition, NI vines suffered more severe water stress and it is known that the amino acids synthesis and accumulation can be influenced by the plant response to stress. According to the results, the irrigation regime showed effect on amino acids concentration in Monastrell grapes under semiarid conditions. Grapes from non-irrigated vines showed a higher content of several amino acids relevant to the fermentative process and to the wine aroma compounds formation. It is demonstrated that the final content of nitrogen-related components in grapes is influenced by the irrigation regime. The convenience of the irrigation strategy to suggest will depend on the desired wine style and the target yield levels.