terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Abstract

Global warming has enhanced the frequency and severity of drought events, hence calling for a better management of water resources in the vineyard and for an improvement of breeding platforms. Somatic embryogenesis (SE) (i.e. the initiation of embryos from somatic tissues) can spontaneously generate new genetic variability, which results from genetic mutations, changes in epigenetic marks, or phenotypic alterations.

This study was tailored to test whether vines in vitro regenerated through SE (i.e. somaclones), can tolerate water deprivation better than the mother plant.

Physiological trials of water stress and recovery were conducted on two populations of different somaclone lines of Vitis vinifera ‘Nebbiolo’ and of 110R (V. rupestris x V. berlandieri) rootstock, respectively regenerated in absence and presence of in vitro selective pressure. During the experiments, dynamic changes in the main eco-physiological parameters were monitored on target somaclones and compared with those measured on plants of the corresponding mother plant lines. Alterations in biometric and anatomical traits were also inspected. The observed responses were further deepened by analyzing differences in the accumulation of defense secondary metabolites and hormones and in the transcription of stress-responsive genes. In parallel, genetic mutations potentially controlling specific physiological adjustments were searched, by sequencing the genomes of the best and worst performing lines.

The integration of physiological, biochemical and molecular data proved that grapevine somaclones are more tolerant to drought and that therefore the exploitation of somaclonal variability can represent an effective and ready-to-use genetic improvement strategy for implementing clonal selection and breeding programs in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Amedeo Moine1*, Paolo Boccacci1, Walter Chitarra1,2, Luca Nerva1,2, Giorgio Gambino1, Irene Perrone1, Chiara Pagliarani1

1 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)

Contact the author*

Keywords

Vitis Spp., water stress, somatic embryogenesis, genetic variability, gas exchange

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The origin and the discovery of “terroir”

Le mot “terroir” dérive du latin “terra”, mais déjà les Romains l’indiquaient comme “locus” ou”loci”, c’est-à-dire un lieu ayant le “genius”destiné à la production d’un produit d’excellente qualité.

Contribution du potentiel glycosidique à l’arôme des vins de Grenache noir et Syrah en Vallée du Rhône

Grenache Noir and Syrah are the predominant grape varieties in the French Rhone valley vineyard, and produce wines with well differentiated aromatic notes. This study aimed at investigating the contribution of glycoconjugated precursors to these aromatic specificities, through their analytical profiles and the sensory influence of the odorant compounds they release during wine aging. The aglycones released by enzymatic hydrolysis of glycosidic extracts

Towards a regional mapping of vine water status based on crowdsourcing observations

Monitoring vine water status is a major challenge for vineyard management because it influences both yield and harvest quality. It is also a challenge at the territorial scale for identifying periods of high water restriction or zones regularly impacted by water stress. This information is of major importance for defining collective strategies, anticipating harvest logistic or applying for irrigation authorisation. At this spatial scale, existing tools and methods for monitoring vine water status are few and often require strong assumptions (e.g. water balance model). This paper proposes to consider a collaborative collection of observations by winegrowers and wine industry stakeholders (crowdsourcing) as an interesting alternative. Indeed, it allows the collection of a large number of field observations while pooling the collection effort. However, the feasibility of such a project and its interest in monitoring vine water status at regional scale has never been tested.

The objective of this article is to explore the possibility of making a regional map of vine water status based on crowdsourcing observations. It is based on the study of the free mobile application ApeX-Vigne, which allows the collection of observations about vine shoot growth. This information is easy to collect and can be considered, under certain conditions, as a proxy for vine water status. This article presents the first results obtained from the nearly 18,000 observations collected by winegrowers and wine industry stakeholders during 2019, 2020 and 2021 seasons. It presents the vine shoot growth maps obtained at regional scale and their evolution over the three vintages studied. It also proposes an analysis of the factors that favoured the number of observations collected and those that favoured their quality. These results open up new perspectives for monitoring vine water status at a regional scale but above they provide references for other crowdsourcing projects in viticulture.

Impact of aging on dimethyl sulfide (DMS) in Corvina and Corvinone wines

Amarone is an Italian red wine produced in the Valpolicella area, in north-eastern Italy. Due to its elaboration with withered grapes, Amarone is a rather unique example of dry red wine. However, there is very limited data so far concerning the volatile composition of commercial Amarone wines, which also undergo a cask aging of 2-4 years before release. The present work aims at characterizing the aroma composition of Amarone and to elucidate the relationships between chemical composition and sensory characters.

From soil to canopy, the diversity of adaptation strategies  to abiotic constraints in grapevine

Climate change is here. One of the main consequences is an increase in the frequency and severity of abiotic stresses which mostly occur in a combined manner. Grapevine, which grows in a large diversity of pedo-climatic conditions, has presumably evolved different mechanisms to allow this widespread adaptation. Harnessing the genetic diversity in these mechanisms will be central to the future of viticulture in many traditional wine growing areas. The interactions between the scion and the rootstock through grafting add an additional level of diversity and adaptive potential to explore.
At the physiological level, these mechanisms are related to processes such as root system development and functioning (water and nutrient uptake), interactions with the soil microbiome, gas exchange regulation, hydraulic properties along the soil-plant-atmosphere continuum, reserve storage, short and long distance signaling mechanisms and plasticity for some of these traits.