Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Recent advancements in genomic sequencing technologies have enabled more detailed and direct studies of DNA methylation, which can help characterise epigenetic variations in plants. The Grapevine Improvement team at the Bragato Research Institute is studying the use of Oxford Nanopore sequencing to identify epigenetic changes associated with environmental differences among clonally-propagated grapevines.

This study involved sequencing DNA from the same Sauvignon Blanc clone, sourced from diverse New Zealand viticultural regions, using the PromethION platform.

Due to the central role of the ripening timing in the evaluation of the varietal response to the environmental resources, a method to manage maturation curves has been developed. The method produces an index of veraison precocity and overcomes several methodological problems, like the visual evaluation of the veraison point and the multi-annual and multi-varieties data processing. It is based on a statistical and mathematical processing of the sugar ripening curves.

In interaction with climate and genetic or human factors, the soil is a major component of the viticulture terroir. The mineral composition of the soil influences vine performance and wine sensory attributes. Among the elements that vines take from the soil, nitrogen is the one that has the strongest impact on vine physiology, vigor and grape composition. In addition to its major effect on primary metabolites in berries, nitrogen plays also a decisive role in the secondary metabolism, especially in the production of key compounds for berries quality, like volatile thiols, methoxypyrazines and glutathione (GSH).

Grapevine rootstocks have traditionally been chosen in order to manage scion vigour, soil pests and soil conditions. Riparia Gloire de Montpellier (RGM) has been in use since the turn of the 19th century, over 100 years and still a remarkably stable source of phylloxera (Daktulosphaeria vitifoliae Fitch) resistance. The original source material was probably collected near the Missouri/Mississippi river confluence, a mid-continental but more southerly location in the United States. It has been hypothesized that more northerly selections of V. riparia Michx might improve both fall acclimation rate and depth of the scion, thus mitigating late fall frost and midwinter freeze damage.

Insufficient acidity in grapes from warm climates/vintages is commonly corrected through addition of tartaric acid during vinification, and less so with other organic acids. An alternative approach involves bio-acidification with the yeast Lachancea thermotolerans (LT) via lactic acid production during fermentation.