terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Abstract

Global warming has enhanced the frequency and severity of drought events, hence calling for a better management of water resources in the vineyard and for an improvement of breeding platforms. Somatic embryogenesis (SE) (i.e. the initiation of embryos from somatic tissues) can spontaneously generate new genetic variability, which results from genetic mutations, changes in epigenetic marks, or phenotypic alterations.

This study was tailored to test whether vines in vitro regenerated through SE (i.e. somaclones), can tolerate water deprivation better than the mother plant.

Physiological trials of water stress and recovery were conducted on two populations of different somaclone lines of Vitis vinifera ‘Nebbiolo’ and of 110R (V. rupestris x V. berlandieri) rootstock, respectively regenerated in absence and presence of in vitro selective pressure. During the experiments, dynamic changes in the main eco-physiological parameters were monitored on target somaclones and compared with those measured on plants of the corresponding mother plant lines. Alterations in biometric and anatomical traits were also inspected. The observed responses were further deepened by analyzing differences in the accumulation of defense secondary metabolites and hormones and in the transcription of stress-responsive genes. In parallel, genetic mutations potentially controlling specific physiological adjustments were searched, by sequencing the genomes of the best and worst performing lines.

The integration of physiological, biochemical and molecular data proved that grapevine somaclones are more tolerant to drought and that therefore the exploitation of somaclonal variability can represent an effective and ready-to-use genetic improvement strategy for implementing clonal selection and breeding programs in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Amedeo Moine1*, Paolo Boccacci1, Walter Chitarra1,2, Luca Nerva1,2, Giorgio Gambino1, Irene Perrone1, Chiara Pagliarani1

1 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)

Contact the author*

Keywords

Vitis Spp., water stress, somatic embryogenesis, genetic variability, gas exchange

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Correction de la teneur en alcool des vins par évaporation partielle sous vide en cours de fermentation alcoolique

Climate change has become a reality that is becoming more and more apparent every day, with changes in the physico-chemical composition of grapes and an increase in the alcohol content of finished wines. These higher alcoholic degrees are not without consequences for the success of alcoholic and malolactic fermentation. Correcting the alcohol content (-20% of the initial alcoholic strength) is also part of an approach designed to meet consumer expectations for healthier, lighter or lower-alcohol wines (9 to 13% vol.). Correcting the alcohol content of wines also rebalances the mouthfeel by reducing the alcohol’s burn.

A preliminary study of clonal selection in cv. Viura in relation to varietal aroma profile

Viura is a synonym for Macabeo and currently it is the most widely planted white grape variety in D.O.Ca. Rioja, with 3,569 ha, representing 84% of the white grape cultivated area. It is a generous-yielding grape, presenting low values of titratable acidity and with large and compact clusters which makes it susceptible to Botrytis cinerea. Thus, this variety not always satisfies the wine grower’s prospects. Nowadays, the available plant material is scarce, moreover, it was selected on the basis of other quality criteria, not currently requested.

Genetic variation among wild grapes native to Japan

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan. A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan.

Progetto di zonazione delle valli di Cembra e dell’Adige. Analisi del comportamento della varietà Pinot nero in ambiente subalpino

Nel 1990 la Cantina LA VIS ha intrapreso un progetto di zonazione dei terreni vitati allo scopo di acquisire le conoscenze scientifiche atte a consentire il miglioramento delle qualità dei prodotti. Tale progetto si è articolato su di una superficie di 2000 ettari ubicati lungo l’asta fluviale del fiume Adige da Trento a Salorno e del torrente Avisio da Lavis a Segonzano.

Effects of the biodynamic preparations 500 and 501 on vine and berry physiology, pedology and the soil microbiome

In the pursuit of increasing sustainability, climate change resiliency and independence of synthetic pesticides in agriculture, the interest of consumers and producers in organic and biodynamic farming is steadily increasing. This is in particular the case for the vitivinicultural industry in Europe, where more and more producers are converting from organic to biodynamic farming. However, clear scientific evidence showing that biodynamic farming improves vine physiology, vine stress resilience, berry or wine quality, or is more sustainable for the environment is still lacking although this issue has been addressed by several research teams worldwide.