terclim by ICS banner
IVES 9 IVES Conference Series 9 Elucidating the biological function of EPFL9 in grapevine roots

Elucidating the biological function of EPFL9 in grapevine roots

Abstract

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance. At the root level, preliminary results showed that edited plants have shorter, but ticker roots compared to WT. In addition, to further analyze the root physiology, biological replicates of two VviEPFL9-2 KO lines and WT were planted in rhizotrons and subject to a water deprivation experiment. Our results will allow us to understand if VviEPFL9-2 might be an interesting target for the application of new genomics techniques in grapevine rootstocks to make them more resilient to drought conditions. If so, a new tool will be available to face the challenges that climate changes pose to viticulture.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Umar Shahbaz1,2, Pierre Videau3, Katerina Labonova3, Stefano Piazza1, Mickael Malnoy1, Olivier Zekri3, Michele Faralli2, Lorenza Dalla Costa1*

1 Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
2 Center Agriculture Food Environment (C3A), University of Trento, 38098, San Michele all’Adige, Italy
3 Novatech, Mercier Groupe, Le Champ des Noels, France

Contact the author*

Keywords

Vitis species, rootstocks, Epidermal Patterning Factors, CRISPR/Cas9, resilience to drought

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

ANTI-TRANSPIRANT MODULATION OF GRAPE RIPENING: EFFECTS ON MERLOT VINE DEVELOPMENT AND ROSÉ WINE PHENOLIC AND AROMATIC PROFILES

Climate changes are impacting viticultural regions throughout the world with temperature increases being most prevalent.1 These changes will not only impact the regions capable of growing grapes, but also
the grapes that can be grown.2 As temperatures rise the growing degree days increase and with it the sugar accumulation within the berries and subsequent alcohol levels in wine. Consequently, viticultural
practices need to be examined to decrease the levels of sugars.

Read More

Baccus: from framework to web platform for self-assessment of wine sustainability

Sustainability is becoming an increasingly present challenge, whether due to an increase in the level of perception and demand from consumers and stakeholders or the rise of events linked to climate change, which directly impacts agricultural-based sectors such as the vine and wine industry.

Read More

Guyot or pergola for dehydration of Rondinella grape

Pergola veronese is the most important vine training system in Valpolicella area but Guyot in the last decades is diffusing. Rondinella is one of the three most important varieties

Read More

Strategies for sample preparation and data handling in GC-MS wine applications

It is often said that wine is a complex matrix and the chemical analysis of wine with the thousands of compounds detected and often measured is proof. New technologies can assist not only in separating and identifying wine compounds, but also in providing information about the sample as a whole. Information-rich techniques can offer a fingerprint of a sample (untargeted analysis), a comprehensive view of its chemical composition. Applying statistical analysis directly to the raw data can significantly reduce the number of compounds to be identified to the ones relevant to a particular scientific question. More data can equal more information, but also more noise for the subsequent statistical handling.

Read More

Geological history and landscape of the Coastal wine-growing region, South Africa

The geology of the Western Cape testifies to the former existence of a late Precambrian supercontinent, its fragmentation, the closure of an ocean between the South African and South American continental precursors (Kalahari and Rio de la Plata cratons), the accumulation of marine sediments and limestones, and their compression during a collision between these cratons

Read More