terclim by ICS banner
IVES 9 IVES Conference Series 9 Elucidating the biological function of EPFL9 in grapevine roots

Elucidating the biological function of EPFL9 in grapevine roots

Abstract

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance. At the root level, preliminary results showed that edited plants have shorter, but ticker roots compared to WT. In addition, to further analyze the root physiology, biological replicates of two VviEPFL9-2 KO lines and WT were planted in rhizotrons and subject to a water deprivation experiment. Our results will allow us to understand if VviEPFL9-2 might be an interesting target for the application of new genomics techniques in grapevine rootstocks to make them more resilient to drought conditions. If so, a new tool will be available to face the challenges that climate changes pose to viticulture.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Umar Shahbaz1,2, Pierre Videau3, Katerina Labonova3, Stefano Piazza1, Mickael Malnoy1, Olivier Zekri3, Michele Faralli2, Lorenza Dalla Costa1*

1 Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
2 Center Agriculture Food Environment (C3A), University of Trento, 38098, San Michele all’Adige, Italy
3 Novatech, Mercier Groupe, Le Champ des Noels, France

Contact the author*

Keywords

Vitis species, rootstocks, Epidermal Patterning Factors, CRISPR/Cas9, resilience to drought

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Different oxygen and sulphur dioxide concentrations in ‘Sauvignon blanc’ must: effect on the composition of the must and wine

The effects of different oxygen and sulphur dioxide additions to South African ‘Sauvignon blanc’ musts were investigated. Oxygen addition without SO2 protection led to lower levels of certain volatile thiols in the wines, with a corresponding decrease in certain phenols and glutathione concentrations.

Advancing wine authentication: non-invasive near-infrared spectroscopy and machine learning for vintage and quality traits assessment

Wine fraud, encompassing counterfeiting and adulteration, poses a significant threat to the wine industry, resulting in annual losses totalling billions of dollars.

Vinhos de talha: to pitch or not to pitch

In Alentejo, south of Portugal there is a traditional way of fermenting wines in clay vessels, known as “Vinhos de Talha”. Clay vessels were traditionally impermeabilized using pine pitch, creating a barrier between the fermenting must and the clay. Due to this unusual production technology that uses of clay vessels, instead of inox or wood vessels, “Vinhos de Talha” present unique characteristics increasingly appreciated by national and international consumers when compared with wine obtained by the said traditional methods of winemaking. Although the positive consumers feedback, there is little literature about the physical-chemical characteristics of these wines (Martins et al, 2018; Cabrita et al, 2018). This work aims to characterize the volatile composition of white wines produced in clay vessels with different coatings and to contribute to the knowledge and preservation of these wines that are a unique cultural heritage. Wine samples were produced during 2019 vintage from white grapes, using the traditional technology associated to these wines.

A deep learning object detection approach for smart pest identification in vineyards

Flavescence dorée (FD) poses a significant threat to grapevine health, with the American grapevine leafhopper, Scaphoideus titanus, serving as the primary vector.

Spotted lanternfly, a new invasive insect in vineyards: is it a threat to grapevines?

The spotted lanternfly (SLF; Lycorma delicatula) is a phloem-feeding polyphagous insect invasive to the Eastern U.S.. Since its first detection in Pennsylvania (U.S.) in 2014, large infestations and economic damage (e.g., decreased yield, vine decline, greater pesticide use) have been reported in an increasing number of vineyards, threatening the sustainability and growth of the wine industry in infested regions. Our team has been investigating the impacts of SLF phloem-feeding on physiological processes, fruit production, juice, and wine composition of different grape cultivars, and also evaluated if the SLF can transmit important grapevine pathogens. In addition, we are working closely with stakeholders to better enumerate the economic damage caused by this pest. These findings will provide relevant information to grape and wine producers to help identify action thresholds and develop a more targeted integrated pest management program.