terclim by ICS banner
IVES 9 IVES Conference Series 9 Estimation of stomatal conductance and chlorophyll fluorescence in Croatian grapevine germplasm under water deficit    

Estimation of stomatal conductance and chlorophyll fluorescence in Croatian grapevine germplasm under water deficit    

Abstract

Water deficit profoundly impacts the quality of grapes and results in considerable reductions in crop yield. First symptoms manifest with reduced stomatal conductance and transpiration, accompanied by the wilting of apical leaves and tendrils. So far, there is no available data on the water stress response in Croatian grapevine germplasm. Therefore, objective of this study was to determine influence of genotype and treatment on stomatal conductance (gsw), transpiration (E), electron transport rate (ETR), and quantum efficiency in light (PhiPS2). In this research we observed the initial response to water deficit of 84 unique genotypes, 70 Vitis vinifera subsp. vinifera and 14 Vitis vinifera subsp. sylvestris accessions. The experiment was conducted in a greenhouse in both 2022 and 2023, involving self-rooted cuttings exposed to water stress and compared to a well-watered control. Multifactorial analysis of variance was used to examine the effects of genotype, treatment, replicate, date and time of measurement on gsw, E, ETR, PhiPS2. In both years gsw and E were significantly influenced by all parameters except replicate, while ETR wasn’t significantly influenced by treatment in second year and PhiPS2 in first year. Due to the observed significance of the interaction between genotype and treatment across all parameters in both years, we employed the pairwise comparisons of treatment levels within each genotype with Bonferroni correction. In this study, a non-destructive high-throughput method for rapid screening of the initial physiological response to water deficit is briefly presented, in which the grapevine genotypes studied are divided into two distinct groups.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luka Marinov1*, Domagoj Ivan Žeravica2, Katarina Lukšić1, Ana Mucalo1, Maja Ozretić Zoković1, Toni Safner3, Goran Zdunić1

1 Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
2 University of Dubrovnik, Dubrovnik, Croatia
3 University of Zagreb, Faculty of Agriculture, Zagreb, Croatia

Contact the author*

Keywords

water stress, genotype, stomatal conductance, sylvestris, vinifera

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

“Terroir” studies in the Côtes du Rhône controlled appellation: from zoning to application

This work gives a summary of the most important programmes about viticultural « terroirs », developed on the « Côtes du Rhône » controlled appellation area for about twenty years.

Studio preliminare sulla microzonazione Bioclimatica condotto in un’area viticola collinare

La caratterizzazione bioclimatica del territorio rappresenta un elemento sempre più impor­tante per il miglioramento dell’ attività agricola. La conoscenza degli andamenti assunti dai parametri meteorologici puà consentire di individuare le peculiarità dei singoli appezzamenti aziendali, ottimizzando le scelte sia in termini tattici (esecuzione dei più opportuni interventi colturali) che strategici (scelta delle specie o varietà più idonee a valorizzare ciascun am­biente).

Wine by-products valorisation by green chemistry methods: Impact of the extraction process on the structure, functionalities and activity of the extracted molecules

Wine by-products valorisation by green chemistry methods: Impact of the extraction process on the structure, functionalities and activity of the extracted molecules

Typicité et terroir : importance relative du type de sol et du niveau de maturité dans la typologie sensorielle du vin

Le lien fonctionnel entre typicité et terroir a été étudié en prenant en compte deux dimensions importantes : le type de sol et la date de vendanges. Ces deux facteurs sont, à des degrés divers

Elucidating the biological function of EPFL9 in grapevine roots

Epidermal Patterning Factors are a class of cysteine rich peptides known to be involved in many developmental processes. The role of EPF1, EPF2 and EPFL9 in controlling leaf stomata formation has been well described in model plants and cereals, and recently also in grapevine, while little is known about their activity in other organs. The aim of our study is to investigate whether VviEPFL9-2 can have a specific biological function in grapevine roots, where it resulted to be expressed. As grapevine is cultivated in the form of a grafted plant, we focused our study on the commonly used rootstock Kober 5BB (Vitis berlandieri x Vitis riparia). VviEPFL9-2 was edited in Kober 5BB plants using Agrobacterium tumefaciens transformation of embryogenic calli and the CRISPR/Cas9 technology. The phenotypic evaluation in greenhouse indicated that, as expected, the leaves of knock-out (KO) plants have a significant lower stomatal density compared to WT, associated with a lower stomatal conductance.