terclim by ICS banner
IVES 9 IVES Conference Series 9 Estimation of stomatal conductance and chlorophyll fluorescence in Croatian grapevine germplasm under water deficit    

Estimation of stomatal conductance and chlorophyll fluorescence in Croatian grapevine germplasm under water deficit    

Abstract

Water deficit profoundly impacts the quality of grapes and results in considerable reductions in crop yield. First symptoms manifest with reduced stomatal conductance and transpiration, accompanied by the wilting of apical leaves and tendrils. So far, there is no available data on the water stress response in Croatian grapevine germplasm. Therefore, objective of this study was to determine influence of genotype and treatment on stomatal conductance (gsw), transpiration (E), electron transport rate (ETR), and quantum efficiency in light (PhiPS2). In this research we observed the initial response to water deficit of 84 unique genotypes, 70 Vitis vinifera subsp. vinifera and 14 Vitis vinifera subsp. sylvestris accessions. The experiment was conducted in a greenhouse in both 2022 and 2023, involving self-rooted cuttings exposed to water stress and compared to a well-watered control. Multifactorial analysis of variance was used to examine the effects of genotype, treatment, replicate, date and time of measurement on gsw, E, ETR, PhiPS2. In both years gsw and E were significantly influenced by all parameters except replicate, while ETR wasn’t significantly influenced by treatment in second year and PhiPS2 in first year. Due to the observed significance of the interaction between genotype and treatment across all parameters in both years, we employed the pairwise comparisons of treatment levels within each genotype with Bonferroni correction. In this study, a non-destructive high-throughput method for rapid screening of the initial physiological response to water deficit is briefly presented, in which the grapevine genotypes studied are divided into two distinct groups.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luka Marinov1*, Domagoj Ivan Žeravica2, Katarina Lukšić1, Ana Mucalo1, Maja Ozretić Zoković1, Toni Safner3, Goran Zdunić1

1 Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
2 University of Dubrovnik, Dubrovnik, Croatia
3 University of Zagreb, Faculty of Agriculture, Zagreb, Croatia

Contact the author*

Keywords

water stress, genotype, stomatal conductance, sylvestris, vinifera

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.

Effect of regulated deficit irrigation regime on amino acids content of Monastrell (Vitis vinifera L.) grapes

Irrigation is an important practice to influence vine quality, especially in Mediterranean regions, characterized by hot summers and severe droughts during the growing season. This study focused on deficit irrigation regime influence on amino acids composition of Monastrell grapevines under semiarid conditions (Albacete, Southeastern of Spain). In 2019, two treatments were applied: non-irrigation (NI) and regulated deficit irrigation (RDI), watered at 30% of the estimated crop evapotranspiration from fruit set to onset of veraison. Grape amino acids content was analyzed by HPLC. Berries from non-irrigated vines showed higher concentration of several amino acids, such as tryptophan (73%), arginine (70%), lysine (36%), isoleucine (27%), and leucine (21%), compared to RDI grapes. Arginine is, together with ammonium ion, the principal nitrogen source for yeasts during the alcoholic fermentation; while isoleucine, tryptophan, and leucine are precursors of fermentative volatile compounds, key compounds for wine quality. Moreover, NI treatment increased in a 14% the total amino acids content in grapes compared to RDI treatment. The reported effects might be because yield was 70% higher in RDI vines than in the NI ones and, therefore, the sink demand was increased in the irrigated vines. In addition, NI vines suffered more severe water stress and it is known that the amino acids synthesis and accumulation can be influenced by the plant response to stress. According to the results, the irrigation regime showed effect on amino acids concentration in Monastrell grapes under semiarid conditions. Grapes from non-irrigated vines showed a higher content of several amino acids relevant to the fermentative process and to the wine aroma compounds formation. It is demonstrated that the final content of nitrogen-related components in grapes is influenced by the irrigation regime. The convenience of the irrigation strategy to suggest will depend on the desired wine style and the target yield levels.

Lactic acid bacteria: A possible aid to the remediation of smoke taint?

With climate change, the occurrence of wildfires has increased in several viticultural regions of the world. Subsequently, smoke taint has become a major issue, threatening the sustainability of the wine industry.

All acids are equal, but some acids are more equal than others: (bio)acidification of wines

Insufficient acidity in grapes from warm(ing) climates is commonly corrected through addition of tartaric acid during vinification, and less so with other organic acids. One alternative approach involves bio-acidification with certain strains of Lachancea thermotolerans (LT) via lactic acid production during fermentation.

Severe infestations of Daktulosphaeria vitifoliae on the hybrid rootstock 1103 Paulsen in Apulia Region (Italy)

In the last four years, despite repeated fertilization and irrigation applications from the farmer, a progressive vegetative decline and yield decrease have been observed in a large (5 ha) 10-year-old table grapes vineyard of the cv. Autumn Pearl grafted on 1103 Paulsen and located nearby the Ionian Sea in Taranto province (Apulia, Italy).