terclim by ICS banner
IVES 9 IVES Conference Series 9 Estimation of stomatal conductance and chlorophyll fluorescence in Croatian grapevine germplasm under water deficit    

Estimation of stomatal conductance and chlorophyll fluorescence in Croatian grapevine germplasm under water deficit    

Abstract

Water deficit profoundly impacts the quality of grapes and results in considerable reductions in crop yield. First symptoms manifest with reduced stomatal conductance and transpiration, accompanied by the wilting of apical leaves and tendrils. So far, there is no available data on the water stress response in Croatian grapevine germplasm. Therefore, objective of this study was to determine influence of genotype and treatment on stomatal conductance (gsw), transpiration (E), electron transport rate (ETR), and quantum efficiency in light (PhiPS2). In this research we observed the initial response to water deficit of 84 unique genotypes, 70 Vitis vinifera subsp. vinifera and 14 Vitis vinifera subsp. sylvestris accessions. The experiment was conducted in a greenhouse in both 2022 and 2023, involving self-rooted cuttings exposed to water stress and compared to a well-watered control. Multifactorial analysis of variance was used to examine the effects of genotype, treatment, replicate, date and time of measurement on gsw, E, ETR, PhiPS2. In both years gsw and E were significantly influenced by all parameters except replicate, while ETR wasn’t significantly influenced by treatment in second year and PhiPS2 in first year. Due to the observed significance of the interaction between genotype and treatment across all parameters in both years, we employed the pairwise comparisons of treatment levels within each genotype with Bonferroni correction. In this study, a non-destructive high-throughput method for rapid screening of the initial physiological response to water deficit is briefly presented, in which the grapevine genotypes studied are divided into two distinct groups.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Luka Marinov1*, Domagoj Ivan Žeravica2, Katarina Lukšić1, Ana Mucalo1, Maja Ozretić Zoković1, Toni Safner3, Goran Zdunić1

1 Institute for Adriatic Crops and Karst Reclamation, Split, Croatia
2 University of Dubrovnik, Dubrovnik, Croatia
3 University of Zagreb, Faculty of Agriculture, Zagreb, Croatia

Contact the author*

Keywords

water stress, genotype, stomatal conductance, sylvestris, vinifera

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

Genomic characterization of terpene biosynthetic genes in seven Vitis vinifera L. varieties 

Grapes (Vitis vinifera L.) are a fruit crop of high economic significance globally. Each grapevine cultivar is characterized by its distinctive grape aroma, affecting the wine quality. In several cultivars, the aroma is shaped by terpenoid (mono- and sesqui-terpenoids). Their profile is controlled by terpene synthases (TPS), which are part of a largely expanded gene family. How the variation in TPS copy number and sequence among cultivars determines terpenoid profiles of grapes remains largely unexplored. We annotated TPS in the haplotypes of seven genomes (Riesling, Albariño, Fiano, Gewürztraminer, Pinot Noir, Cabernet Sauvignon, and Viognier) using BLAST, GMAP, PFAM, and phylogenetic analyses. Further, TPS expression patterns and terpenoid accumulation during berry development and ripening were characterized using RNA-Seq and SPME/GC-MS platforms, respectively. Variation in TPS copy number exists among cultivars. Specifically, the TPS counts span a range of 251 to 150 for Riesling and Fiano, respectively, when considering combined haplotypes within each cultivar. Total terpenoid accumulation patterns throughout development were consistent among the five aromatic cultivars, marked by high concentrations in flowers, followed by a decline and subsequent rise during berry development and ripening, respectively. Conversely, non-aromatic cultivars exhibited no substantial increase in terpenoid concentration during ripening. Transcriptome and network analyses are currently employed to determine which TPS are expressed in the berry and determine the terpenoid profile of the specific cultivar. These findings shed light on the genomic determinants of grape aroma in major cultivars, and allow future studies focused on cultivar-specific responses of terpenoid biosynthesis to environmental stresses.

The evolution of wine appellations in the United States

Le système des appellations d’origine aux Etats-Unis était adopté en 1978 et est entré en vigueur en 1983. Jusqu’à présent, 146 aires viticoles avaient été établies dans 26 états.

Volatile and phenolic profiles of wines closed with different stoppers and stored for 30 months

The aim of this study was to evaluate the volatile and phenolic profiles of three red and one rosé wines stored in bottles for 30 months. Four wines were provided by a winery located in South Tyrol

Digitising the vineyard: developing new technologies for viticulture in Australia 

New and developing technologies, that provide sensors and the software systems for using and interpreting them, are becoming pervasive through our lives and society. From smart phones to cars to farm machinery, all contain a range of sensors that are monitored automatically with intelligent software, providing us with the information we need, when we need it. This technological revolution has the potential to monitor all aspects of vineyard activity, assisting growers to make the management choices they need to achieve the outcomes they want. For example, a future vineyard may possess automated imaging that generates a three dimensional model of the vine canopy, highlighting differences from the desired structure and how to use canopy management to improve fruit composition, or generates maps with yield estimates and measurements of berry composition throughout the growing season.