terclim by ICS banner
IVES 9 IVES Conference Series 9 Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Abstract

Downy mildew, a disease caused by Plasmopara viticola (Berk. et Curt.) Berl. and De Toni, is one of the strongest threats to grapevine (Vitis vinifera L.) production. Recently, sources of resistance to downy mildew were identified among Caucasian germplasm. Among them, the Georgian variety Mgaloblishvili revealed a unique resistance mechanism. A genome wide association study (GWAS) allowed the identification of the genetic bases of Mgaloblishvili resistance, the loci Rpv29, Rpv30 and Rpv31. To dissect the three resistance loci, Mgaloblishvili genome was sequenced using PacBio HiFi reads and assembled. A chromosome-scale diploid genome assembly consisting of ~ 986 Mb and a contig N50 length of 25.8 Mb was obtained. A total of 58,912 protein-coding genes were predicted on the two sets of phased chromosomes. A whole genome comparison with the genome of the susceptible reference accession PN40024 was performed. Mgaloblishvili resistance loci were in-depth analyzed in terms of structure, gene content, gene expression and impact of structural variants (SVs) and SNPs (Single Nucleotide Polymorphisms). Furthermore, using DNA sequencing data of Mgaloblishvili self-cross progeny, resistance haplotypes were identified for Rpv30 and Rpv31. The obtained data highlighted Mgaloblishvili resistant phenotype as a consequence of multiple small SVs and SNPs, that eventually results into differential transcriptional regulation. Altogether, these genetic resources will increase the knowledge about downy mildew-grapevine pathosystem. Moreover, they will be available for breeding programs aiming to develop grapevine resistant varieties.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Valentina Ricciardi 1, Andrea Minio 2, Melanie Massonnet 2, Alexander H.J. Wittenberg3, David Maghradze 4,5,6, Silvia Laura Toffolatti 1, Osvaldo Failla 1, Dario Cantù 2*, Gabriella De Lorenzis

1 Department of Agricultural and Environmental Sciences, University of Milan
2 Department of Viticulture and Enology, University of California, Davis (CA), USA
3 KeyGene, Wageningen, The Netherlands
4 Caucasus International University, Tbilisi, Georgia
5 Georgian Technical University, Tbilisi, Georgia
6National Wine Agency of Georgia, Tbilisi, Georgia

Contact the author*

Keywords

Grapevine, biotic stress, QTL, genome, Caucasus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Vineyard management for environment valorisation

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...
Read More

“Compost Application in the Vineyard: Effects on Soil Nutrition and Compaction”

The mechanization of pruning and harvesting in vineyards has increased the risk of soil compaction. To reclaim soil properties or avoid this degradation process, it is crucial to properly manage the soil organic matter, and the application of compost derived from the vines themselves is a strategy to achieve this. The objective of this study was to evaluate the properties of soil treated with different doses of compost applied both on the vine row and the inter rows of a Vitis vinifera crop.

Read More

The challenge of viticultural landscapes

Le monde vitivinicole est de plus en plus concerné par la question paysagère : l’enjeu est de taille puisqu’il s’agit de la survie de l’image positive dont bénéficient les Appellations d’Origine Contrôlée. Les paysages sont composés d’éléments qui renvoient à des références socioculturelles fortes, susceptibles de modeler l’image d’un produit et d’en déterminer à notoriété et le prix. Dans un monde médiatisé comme le nôtre, le visuel construit l’arrière-plan des représentations mentales associées à toute marchandise ; et pour les aliments, produits de la terre, ce visuel est forcément paysager.

Read More

Mechanistic insights into the bioavailability of oleocanthal and oleacein from olive oil in presence of wine active peptides and amino acids

Oleocanthal (OC) and oleacein (OL) are highly bioactive secoiridoids found in olive oil at elevated concentrations, especially when it is produced from unripe olives (Olea europaea L.). Both compounds have been correlated with strong activities against serious diseases through recent clinical trials. The most important clinical trials have been performed in patients against chronic lymphocytic

Read More

Influence of protein stabilization with aspergillopepsin I on wine aroma composition

The protein haze formation in white and rosé wines during storage, shipping and commercialization has always been an important issue for winemakers. Among the various solutions industrially proposed, the use of bentonite is certainly the most widespread. However, the harmful effects of this treatment are known either in terms of wine volume loss and wine flavour and aroma.

Read More