terclim by ICS banner
IVES 9 IVES Conference Series 9 Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Abstract

Downy mildew, a disease caused by Plasmopara viticola (Berk. et Curt.) Berl. and De Toni, is one of the strongest threats to grapevine (Vitis vinifera L.) production. Recently, sources of resistance to downy mildew were identified among Caucasian germplasm. Among them, the Georgian variety Mgaloblishvili revealed a unique resistance mechanism. A genome wide association study (GWAS) allowed the identification of the genetic bases of Mgaloblishvili resistance, the loci Rpv29, Rpv30 and Rpv31. To dissect the three resistance loci, Mgaloblishvili genome was sequenced using PacBio HiFi reads and assembled. A chromosome-scale diploid genome assembly consisting of ~ 986 Mb and a contig N50 length of 25.8 Mb was obtained. A total of 58,912 protein-coding genes were predicted on the two sets of phased chromosomes. A whole genome comparison with the genome of the susceptible reference accession PN40024 was performed. Mgaloblishvili resistance loci were in-depth analyzed in terms of structure, gene content, gene expression and impact of structural variants (SVs) and SNPs (Single Nucleotide Polymorphisms). Furthermore, using DNA sequencing data of Mgaloblishvili self-cross progeny, resistance haplotypes were identified for Rpv30 and Rpv31. The obtained data highlighted Mgaloblishvili resistant phenotype as a consequence of multiple small SVs and SNPs, that eventually results into differential transcriptional regulation. Altogether, these genetic resources will increase the knowledge about downy mildew-grapevine pathosystem. Moreover, they will be available for breeding programs aiming to develop grapevine resistant varieties.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Valentina Ricciardi 1, Andrea Minio 2, Melanie Massonnet 2, Alexander H.J. Wittenberg3, David Maghradze 4,5,6, Silvia Laura Toffolatti 1, Osvaldo Failla 1, Dario Cantù 2*, Gabriella De Lorenzis

1 Department of Agricultural and Environmental Sciences, University of Milan
2 Department of Viticulture and Enology, University of California, Davis (CA), USA
3 KeyGene, Wageningen, The Netherlands
4 Caucasus International University, Tbilisi, Georgia
5 Georgian Technical University, Tbilisi, Georgia
6National Wine Agency of Georgia, Tbilisi, Georgia

Contact the author*

Keywords

Grapevine, biotic stress, QTL, genome, Caucasus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The impact of differences in soil texture within a vineyard on vine development and wine quality

Marlborough Sauvignon Blanc has rapidly gained an international reputation for style and quality. The extent to which this can be attributed to the climate, soils or vineyard management is at present unclear. However, the young alluvial soils of the Wairau Plains are considered to play an important role in determining this unique wine style. Marked changes in soil texture occur on the Wairau Plains over short distances.

A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

New Plant Breeding Techniques (NPBTs) have arisen with the objective of surmounting the constraints inherent in conventional breeding methodologies, thereby enhancing plant resilience against both biotic and abiotic stresses. To date the application of genome editing in grapevine is still limited by the necessity to overcome recalcitrance to produce embryogenic calli and to regenerate plants. In our studies, we developed a smart and versatile genetic transformation system carrying all the most promising features of different genome editing approaches. In specific, we joined the GRF-GIF expression to improve regeneration, the systemic movement of the editing transcripts through tRNA-like sequences (TLS) and the cisgenic-like approach to remove transgenes.

Mechanisms responsible for different susceptibility of grapevine varieties to flavescence dorée

Flavescence dorée (FD) is the most serious grapevine yellows disease in Europe. It is caused by phytoplasmas which are transmitted from grapevine to grapevine by the leafhopper Scaphoideus titanus.

Utilisation de données historiques pour caractériser le millésime en cours

Cet article propose la formalisation d’un modèle paramétrique pour représenter l’accumulation des sucres dans les baies de raisin durant la maturation. Le test de ce modèle sur des jeux de données réels a permis de valider l’approche proposée. Une seconde partie est axée sur l’adaptation de la méthode pour permettre la simulation du comportement du millésime en cours dès les premiers relevés de maturité. Ce travail possède de multiples applications dans le domaine de l’aide à la décision.

Impact of Japanese beetles (Popillia japonica Newman) on the chemical composition of two grape varieties grown in Italy (Nebbiolo and Erbaluce)

The Japanese beetle, Popillia japonica Newman, is considered one of the most harmful organisms due to its ability to feed on more than 300 plant species. Symptoms indicative of adult beetles include feeding holes in host plants extending to skeletonization of leaves when population numbers are high. The vine is one of the species most affected by this beetle. However, the damaged plants, even if with difficulty, manage to recover, bringing the bunches of grapes to ripeness.