terclim by ICS banner
IVES 9 IVES Conference Series 9 Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Abstract

Downy mildew, a disease caused by Plasmopara viticola (Berk. et Curt.) Berl. and De Toni, is one of the strongest threats to grapevine (Vitis vinifera L.) production. Recently, sources of resistance to downy mildew were identified among Caucasian germplasm. Among them, the Georgian variety Mgaloblishvili revealed a unique resistance mechanism. A genome wide association study (GWAS) allowed the identification of the genetic bases of Mgaloblishvili resistance, the loci Rpv29, Rpv30 and Rpv31. To dissect the three resistance loci, Mgaloblishvili genome was sequenced using PacBio HiFi reads and assembled. A chromosome-scale diploid genome assembly consisting of ~ 986 Mb and a contig N50 length of 25.8 Mb was obtained. A total of 58,912 protein-coding genes were predicted on the two sets of phased chromosomes. A whole genome comparison with the genome of the susceptible reference accession PN40024 was performed. Mgaloblishvili resistance loci were in-depth analyzed in terms of structure, gene content, gene expression and impact of structural variants (SVs) and SNPs (Single Nucleotide Polymorphisms). Furthermore, using DNA sequencing data of Mgaloblishvili self-cross progeny, resistance haplotypes were identified for Rpv30 and Rpv31. The obtained data highlighted Mgaloblishvili resistant phenotype as a consequence of multiple small SVs and SNPs, that eventually results into differential transcriptional regulation. Altogether, these genetic resources will increase the knowledge about downy mildew-grapevine pathosystem. Moreover, they will be available for breeding programs aiming to develop grapevine resistant varieties.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Valentina Ricciardi 1, Andrea Minio 2, Melanie Massonnet 2, Alexander H.J. Wittenberg3, David Maghradze 4,5,6, Silvia Laura Toffolatti 1, Osvaldo Failla 1, Dario Cantù 2*, Gabriella De Lorenzis

1 Department of Agricultural and Environmental Sciences, University of Milan
2 Department of Viticulture and Enology, University of California, Davis (CA), USA
3 KeyGene, Wageningen, The Netherlands
4 Caucasus International University, Tbilisi, Georgia
5 Georgian Technical University, Tbilisi, Georgia
6National Wine Agency of Georgia, Tbilisi, Georgia

Contact the author*

Keywords

Grapevine, biotic stress, QTL, genome, Caucasus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Alimentary film to reduce cork taint and improve wine organoleptic quality

Wine quality may be compromised by mouldy off‒flavours related to cork taint. Although different compounds are considered to be involved in this wine defect, haloanisoles (HAs), and among them the 2,4,6-trichloroanisole (TCA), are claimed as the main responsible.

Read More

Enological evaluation of the attitude of the grapevine fumin to give varietal wines

Initiatives have been ongoing in recent years to safeguard biodiversity in the oenological sector via a process of enhancement of ancient varieties, under a pressure of a market strongly oriented towards production deriving from native vines of specific geographical zones. In that sense, Aosta Valley
(Italy) has raised the need to preserve and characterize its minority vine varieties which have the potentiality to give varietal wines. Fumin represents the 7% of the production of the region with 16 hectares of vineyards and 753 hectolitres of derived wine. Due to its large phenolic potential, strong astringency and deep colour, it has long been, and is still today, assembled or blended with other varieties as occurs, for example, for the Torrette.

Read More

Effect Of Grape Polysaccharides On The Volatile Composition Of Red Wines

Yeast mannoproteins and derivates are polysaccharides produced from the cell walls of different yeast strains widely used in the winemaking and finning of wines to improve their overall stability and sensory properties.

Read More

Characterized one of the largest collections of grapevine rootstocks (non-vinifera)

Microsatellite markers are a valuable tool to facilitate the management of germplasm collections and assess genetic diversity. This study reports the genetic characterization of a large collection of 379 rootstocks and other non-viniferaaccessions maintained at the University of Milan, Italy.

Read More

New methods and technologies to describe the environment in terroir studies

The concept of terroir in viticulture deals with the influence of environmental factors on vine behaviour and grape ripening. Recent advances in technology, in particular computer technology, allow a more in-depth study of the environment. Geomorphology can be studied with digital Elevation Models (DEM). Soils can be surveyed with geophysics.

Read More