terclim by ICS banner
IVES 9 IVES Conference Series 9 Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Mgaloblishvili Rpv29 and Rpv31 loci reveal new insights on downy mildew resistance sources in Vitis vinifera

Abstract

Downy mildew, a disease caused by Plasmopara viticola (Berk. et Curt.) Berl. and De Toni, is one of the strongest threats to grapevine (Vitis vinifera L.) production. Recently, sources of resistance to downy mildew were identified among Caucasian germplasm. Among them, the Georgian variety Mgaloblishvili revealed a unique resistance mechanism. A genome wide association study (GWAS) allowed the identification of the genetic bases of Mgaloblishvili resistance, the loci Rpv29, Rpv30 and Rpv31. To dissect the three resistance loci, Mgaloblishvili genome was sequenced using PacBio HiFi reads and assembled. A chromosome-scale diploid genome assembly consisting of ~ 986 Mb and a contig N50 length of 25.8 Mb was obtained. A total of 58,912 protein-coding genes were predicted on the two sets of phased chromosomes. A whole genome comparison with the genome of the susceptible reference accession PN40024 was performed. Mgaloblishvili resistance loci were in-depth analyzed in terms of structure, gene content, gene expression and impact of structural variants (SVs) and SNPs (Single Nucleotide Polymorphisms). Furthermore, using DNA sequencing data of Mgaloblishvili self-cross progeny, resistance haplotypes were identified for Rpv30 and Rpv31. The obtained data highlighted Mgaloblishvili resistant phenotype as a consequence of multiple small SVs and SNPs, that eventually results into differential transcriptional regulation. Altogether, these genetic resources will increase the knowledge about downy mildew-grapevine pathosystem. Moreover, they will be available for breeding programs aiming to develop grapevine resistant varieties.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Valentina Ricciardi 1, Andrea Minio 2, Melanie Massonnet 2, Alexander H.J. Wittenberg3, David Maghradze 4,5,6, Silvia Laura Toffolatti 1, Osvaldo Failla 1, Dario Cantù 2*, Gabriella De Lorenzis

1 Department of Agricultural and Environmental Sciences, University of Milan
2 Department of Viticulture and Enology, University of California, Davis (CA), USA
3 KeyGene, Wageningen, The Netherlands
4 Caucasus International University, Tbilisi, Georgia
5 Georgian Technical University, Tbilisi, Georgia
6National Wine Agency of Georgia, Tbilisi, Georgia

Contact the author*

Keywords

Grapevine, biotic stress, QTL, genome, Caucasus

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Know thy enemy: oxygen or storage temperature?

It is well known that high oxygen levels and high ageing temperatures are detrimental to white wine’s composition and ageing capacity. However, these results, though valuable

Effects of wine versus de-alcoholised wine on the microbiota-gut-brain axis in a tau-pathology murine model of Alzheimer’s disease

Alzheimer’s Disease (AD) is the most common disorder associated with cognitive impairment and the main cause of dementia globally. Multiple evidence in the last decade suggest that the gut microbiome plays an important role in the pathogenesis and progression of AD via the microbiota-gut-brain axis, a network wherein microbiome and the central nervous system crosstalk via endocrine, immune, neural, and microbial metabolites signalling pathways.

Effects of different crop load and pruning aplications on vi̇ne growing, grape yi̇eld and quality parameters of early sweet (Vitis vinifera L.) grape variety

It is important to examine the yield quality elements of table grape varieties. There are great differences in winter and summer pruning of the early sweet grape variety. For this reason, in the study, the effects of different crop loads and pruning processes on grape yield, quality characteristics and vine development in the early sweet (vitis vinifera L.) Grape variety were investigated.

Designing and managing a sustainable vineyard in a climate change scenario

Extension of the growing season, compression of the annual growth cycle and higher frequency and severity of weather extreme events are consistent features of global warming. While mitigation of factors causing global warming is necessary in the medium-long term, wine growers need “ready to go” adaptation practices to counteract negative effects bound to climate change. This must be done in a sustainably way, meaning that remunerative yield, desired grape quality, low production cost and environment friendly solutions must be effectively merged. In this work, we will review contribution given over the last two decades prioritizing issues related to scion and rootstock choice, changes in vineyard floor management, new perception related to the use of irrigation in vineyards, adaptation practices aimed at decompress maturity, solutions to counteract or minimize damages due to late frost and sunburn and, lastly, some hints on how precision viticulture can help with all of this.

Colored hail‐nets as a tool to improve vine water status: effects on leaf gas exchange and berry quality in Italia table grape

Protecting table grape vineyards with white hail‐nets is a common practice in Southern Italy. Hail‐nets result in shading effects of 10‐20 %, depending on their density