terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring diversity of grapevine responses to Flavescence dorée infection

Exploring diversity of grapevine responses to Flavescence dorée infection

Abstract

Flavescence dorée, a serious threat to grapevine cultivation in several European Countries, is caused by phytoplasmas in the 16Sr-V ribosomal group, classified as quarantine organisms in the EU and transmitted mainly by the insect vector Scaphoideus titanus. The disease is controlled only by indirect and preventive measures, with important economic and environmental concerns. Genetic resources from the great variety of Vitis vinifera germplasm together with application of new genomic techniques could be applied to produce resistant/tolerant plants, once the genetic bases of susceptibility are elucidated. In a current Italian project (BIORES*) we are evaluating different international and local grapevine cvs. as well as microvine plants for their response to FD transmission and multiplication in controlled conditions. Infective vectors were caged onto potted plants of 8 varieties for 5 weeks. Insect survival and infectivity were monitored and plant infections were quantitatively tested 11 weeks post inoculation.
In parallel, previous data and materials generated by the Project Consortium are being mined to select putative resistance- or susceptibility-related genes, to be further validated in functional analyses, either on already produced transgenic plants or in future genome editing experiments to introduce precise targeted mutations in candidate genes of the highly susceptible cv. Chardonnay, with the final goal of reducing FD susceptibility without compromising other productive traits.

*Funded by the European Union – NextGenerationEU.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Francesca Cavagna1, Enea Guerrieri1, Davide Danzi1, Sabrina Palmano2, Cristina Marzachì2, Nicola Mori1, Annalisa Polverari1*

1 Department of Biotechnology, University of Verona, via della Pieve 70, San Pietro in Cariano, Verona, Italy
2 Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy

Contact the author*

Keywords

Phytoplasma, Germplasm, Breeding, Genome editing, Sustainable viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Creativini: an augmented reality card game to promote the learning of the reasoning process of a technical management route for making wine 

Nowadays, the entire viticultural and enological process is wisely thought out according to the style of wine to be produced and the local climatic conditions. Acquiring the approach of a technical management route specific for wine production remains a complex learning process for students. To enhance such learning, The Ecole d’Ingénieurs de PURPAN (PURPAN), an engineering school located in Toulouse southwest France, has recently developed Creativini, a collaborative card game in English made of 150 cards spread into 14 batches. Students in groups of 3 to 6 must design a technical production route, from plant material to bottling.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties

The effects of climate change in viticulture are currently a major concern, with heat waves and drought affecting yield, wine quality, and in extreme cases, even plant survival. Ancient grapevine varieties have high intravarietal genetic variability that so far has been explored successfully to improve yield and must quality. Currently, there is little information available on intravarietal variability regarding responses to stress. In the current work, the intravarietal genetic variability of several Portuguese varieties was studied for yield, must quality, and tolerance to abiotic stress, through indirect, rapid, and nondestructive measurements carried out in the field.

Pedological criteria according to the French hierarchy of vintages, Appellations d’Origine Contrôlée (AOC): study of two toposequences located in the Burgundian “Côte”

The concept of terroir is defined by a set of natural and human factors. On the slopy vineyards of the Burgundian « Côte », the « Appellations d’Origine Contrôlée (AOC) » spread out according to the slope in their order of quality : « AOC Grand Cru » at the top, « AOC Premier Cru » and « AOC Village » and « Bourgogne » on the piemont. In order to correlate the hierarchy of the vintages with the evolution of the topographic and pedological criteria, two toposequences were studied, in Gevrey Chambertin (« Côte de Nuits ») and Aloxe Corton (« Côte de Beaune »).

Effects of the addition of yeast derived products during aging in chardonnay sparkling winemaking

From the beginning of the yeast autolysis process, several interesting intracellular and cell wall constituyents are released to the media providing different characteristics to the wine, being this process extensively studied in sparkling wines due to their important contribution to their properties (1-2). Yeast derived products (YDs) try to emulate the natural yeast autolysis compounds release enhancing the organoleptic characteristics of resulting wines (2-3). This study is a comprehensive evaluation of the impact of the addition of different YDs added to base wine on the chemical, physical and sensory characteristics of the resulting sparkling wines. METHODS: Chardonnay base wine was employed to carry out this study. Three experimental YDs were added at 5 and 10 g/hL to the tirage liqueur: a yeast autolysate (YA), a yeast protein extract (PE) and an inactivated dry yeast from Torulaspora delbrueckii, (TD), and two commercial specific inactivated dry yeast: OPTIMUM WHITE® (OW) and PURE-LONGEVITY®(PL). After second fermentation, measurements were carried out after 3, 6, 9 and 18 months of aging on lees. General enological parameters, proteins, polysaccharides (HPLC-DAD-RID), volatile compounds profile (GC-MS), foaming characteristics (Mosalux), and descriptive sensory analyses were carried out.