terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring diversity of grapevine responses to Flavescence dorée infection

Exploring diversity of grapevine responses to Flavescence dorée infection

Abstract

Flavescence dorée, a serious threat to grapevine cultivation in several European Countries, is caused by phytoplasmas in the 16Sr-V ribosomal group, classified as quarantine organisms in the EU and transmitted mainly by the insect vector Scaphoideus titanus. The disease is controlled only by indirect and preventive measures, with important economic and environmental concerns. Genetic resources from the great variety of Vitis vinifera germplasm together with application of new genomic techniques could be applied to produce resistant/tolerant plants, once the genetic bases of susceptibility are elucidated. In a current Italian project (BIORES*) we are evaluating different international and local grapevine cvs. as well as microvine plants for their response to FD transmission and multiplication in controlled conditions. Infective vectors were caged onto potted plants of 8 varieties for 5 weeks. Insect survival and infectivity were monitored and plant infections were quantitatively tested 11 weeks post inoculation.
In parallel, previous data and materials generated by the Project Consortium are being mined to select putative resistance- or susceptibility-related genes, to be further validated in functional analyses, either on already produced transgenic plants or in future genome editing experiments to introduce precise targeted mutations in candidate genes of the highly susceptible cv. Chardonnay, with the final goal of reducing FD susceptibility without compromising other productive traits.

*Funded by the European Union – NextGenerationEU.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Francesca Cavagna1, Enea Guerrieri1, Davide Danzi1, Sabrina Palmano2, Cristina Marzachì2, Nicola Mori1, Annalisa Polverari1*

1 Department of Biotechnology, University of Verona, via della Pieve 70, San Pietro in Cariano, Verona, Italy
2 Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy

Contact the author*

Keywords

Phytoplasma, Germplasm, Breeding, Genome editing, Sustainable viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Emerging pest pressures in viticulture: a brief review of Argyrotaenia Ljungiana in Eastern Europe

As viticulture faces increasing threats from emerging pests, understanding and dealing with new infestations is crucial.

Impact of soil characteristics on grape composition of Tempranillo variety under different weather conditions in Rioja DOCa (Spain)

Aims: The objective of this research was to analyse the spatial and temporal variability of vine phenology of the Tempranillo variety in the Toro Designation of Origen (DO) related to climatic conditions at present and under future climate change scenarios.

Unraveling vineyard site from vintage contributions: Elemental composition of site-specific Pinot noir wines across multiple vintages

Understanding vineyard site contribution to elemental composition of wines has, historically, been limited due to lack of continuity across multiple vintages, as well as lack of uniformity in scion clone and lack of controlled pilot-scale winemaking conditions.  We recently completed our fifth vintage, and have elemental composition characterizing wines from four vintages (2015–2018)

Green berries on Gewürztraminer (Vitis vinifera L.) in South Tyrol (Italy)

The grape variety Gewürztraminer is known to be affected by two physiological disorders namely berry shrivel and bunch stem necrosis. During the season 2014 we noticed a new symptomatology type of ripening disorder on the variety. The new symptom showed not all berries fallowing the normal maturation stages, but single berries remaining at a soft but green stage till harvest. The broad distribution of these so called “green berries” symptoms in different production sites of our region, caused huge damage due to the difficulty of eliminating single berries per bunch before harvesting. Therefore, the Research Centre Laimburg began to investigate the reasons and origins of this new symptom. This work shows the results of first attempts to find causes for the symptom as well as the resulting approach to mitigate symptoms. Applications of magnesium leaf fertilizer showed first promising results against this putative disorder. To study the causal effect of the green berries 30 symptomatic vineyards in 2014 have been selected for a monitoring during the season 2016. To evaluate the foliar nutrient treatment two vineyards have been selected for application of magnesium sulfate and magnesium chloride. Leaf and berry nutrient analysis, as well as the main quality parameters during ripening have been performed. As soon as “green berries” symptoms appeared, incidence and severity have been evaluated. Most of the symptomatic vineyards of the 2016 monitoring showed light to clear magnesium deficit symptoms on their foliage. Only during the seasons 2020 and 2021 “green berries” symptoms could be found in the leaf fertilizer treatment vineyards. Both seasons showed a significant effect of the magnesium treatments to reduce the incidence and severity of the symptom. It seems that the appearance of the “green berries” symptom on Gewürztraminer is correlated to a disturbed uptake of magnesium of the vines.

Soil incorporation of new superabsorbent hydrogels to improve vine tolerance to summer stress: physiological validation and vineyard applications

Hydrogels are soil-conditioning materials capable of absorbing substantial amounts of water relative to their weight.