terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring diversity of grapevine responses to Flavescence dorée infection

Exploring diversity of grapevine responses to Flavescence dorée infection

Abstract

Flavescence dorée, a serious threat to grapevine cultivation in several European Countries, is caused by phytoplasmas in the 16Sr-V ribosomal group, classified as quarantine organisms in the EU and transmitted mainly by the insect vector Scaphoideus titanus. The disease is controlled only by indirect and preventive measures, with important economic and environmental concerns. Genetic resources from the great variety of Vitis vinifera germplasm together with application of new genomic techniques could be applied to produce resistant/tolerant plants, once the genetic bases of susceptibility are elucidated. In a current Italian project (BIORES*) we are evaluating different international and local grapevine cvs. as well as microvine plants for their response to FD transmission and multiplication in controlled conditions. Infective vectors were caged onto potted plants of 8 varieties for 5 weeks. Insect survival and infectivity were monitored and plant infections were quantitatively tested 11 weeks post inoculation.
In parallel, previous data and materials generated by the Project Consortium are being mined to select putative resistance- or susceptibility-related genes, to be further validated in functional analyses, either on already produced transgenic plants or in future genome editing experiments to introduce precise targeted mutations in candidate genes of the highly susceptible cv. Chardonnay, with the final goal of reducing FD susceptibility without compromising other productive traits.

*Funded by the European Union – NextGenerationEU.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Francesca Cavagna1, Enea Guerrieri1, Davide Danzi1, Sabrina Palmano2, Cristina Marzachì2, Nicola Mori1, Annalisa Polverari1*

1 Department of Biotechnology, University of Verona, via della Pieve 70, San Pietro in Cariano, Verona, Italy
2 Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy

Contact the author*

Keywords

Phytoplasma, Germplasm, Breeding, Genome editing, Sustainable viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of ethanol removal on wine composition: Nero d’Avola as case study

Over the past twenty years, climate change has increased the sugar content of grape must, leading to higher ethanol concentrations in wines.

Identification and sensory properties of novel 3-sulfanylhexan-1-ol esters in wine

Polyfunctional thiols are among the most potent aroma compounds in wine and play an important role in the varietal character of a wide range of white and red wines, especially Sauvignon blanc wines.

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

The influence of initial phenolic content on the outcome of pinot noir wine microoxygenation

Over the years, microoxygenation (MOX) has become a popular vinification technique to improve wine sensory qualities. However, among the impacting factors reported

A multivariate clustering approach for a gis based territorial characterization of the montepulciano d’abruzzo DOCG “Colline Teramane”

The aim of the project was to characterize the Premium Denomination of Guaranteed Origin (DOCG) “Colline Teramane” wine-growing region and to delineate and define homogeneous zones (terroir units) within it, by applying a multivariate clustering approach combined with geomatics.