terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring diversity of grapevine responses to Flavescence dorée infection

Exploring diversity of grapevine responses to Flavescence dorée infection

Abstract

Flavescence dorée, a serious threat to grapevine cultivation in several European Countries, is caused by phytoplasmas in the 16Sr-V ribosomal group, classified as quarantine organisms in the EU and transmitted mainly by the insect vector Scaphoideus titanus. The disease is controlled only by indirect and preventive measures, with important economic and environmental concerns. Genetic resources from the great variety of Vitis vinifera germplasm together with application of new genomic techniques could be applied to produce resistant/tolerant plants, once the genetic bases of susceptibility are elucidated. In a current Italian project (BIORES*) we are evaluating different international and local grapevine cvs. as well as microvine plants for their response to FD transmission and multiplication in controlled conditions. Infective vectors were caged onto potted plants of 8 varieties for 5 weeks. Insect survival and infectivity were monitored and plant infections were quantitatively tested 11 weeks post inoculation.
In parallel, previous data and materials generated by the Project Consortium are being mined to select putative resistance- or susceptibility-related genes, to be further validated in functional analyses, either on already produced transgenic plants or in future genome editing experiments to introduce precise targeted mutations in candidate genes of the highly susceptible cv. Chardonnay, with the final goal of reducing FD susceptibility without compromising other productive traits.

*Funded by the European Union – NextGenerationEU.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Francesca Cavagna1, Enea Guerrieri1, Davide Danzi1, Sabrina Palmano2, Cristina Marzachì2, Nicola Mori1, Annalisa Polverari1*

1 Department of Biotechnology, University of Verona, via della Pieve 70, San Pietro in Cariano, Verona, Italy
2 Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy

Contact the author*

Keywords

Phytoplasma, Germplasm, Breeding, Genome editing, Sustainable viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Colloids in red wines: new insights from recent research

Despite their significant impact on wine quality and stability, colloids in red wine remain relatively under-researched. A series of studies, developed in the context of the d-wines project, aimed to provide a comprehensive understanding of the structure, composition, and formation mechanisms of red wine colloids by studying monovarietal wines from 10 of the most significant Italian red grape varieties. Starting from the idea that proteins, polysaccharides, and tannins should be involved in colloid formation, 110 monovarietal red wines were analysed for these components, revealing high inter- and intra-varietal diversity [1].

NEAR INFRARED SPECTROSCOPY FOR THE ESTIMATION OF TEMPRANILLO BLANCO VOLATILE COMPOSITION ALONG GRAPE MATURATION

Grape volatile compounds are mainly responsible for wine aroma, so it is important to know the va-rietal aromatic composition throughout ripening process. Currently, there are no tools that allow mea-suring the aromatic composition of grapes, in intact berries and periodically, throughout ripening, in the vineyard or in the winery. For this reason, this work evaluated the use of near infrared spectroscopy (NIR) to estimate the aromatic composition and total soluble solids (TSS) of Tempranillo Blanco berries during ripening. For this purpose, NIR spectra (1100-2100 nm) were acquired from 240 samples of in-tact berries, collected at different dates, from veraison to overripening.

Protein extracts of the Andean pseudocereals quinoa and kiwicha as alternatives for the fining of wine phenolics.

INTRODUCTION: Lately, there has been an increasing interest in using plant-derived proteins for wine phenolic fining.

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.