terclim by ICS banner
IVES 9 IVES Conference Series 9 Selection of beneficial endophytes from Sicilian grapevine germplasm 

Selection of beneficial endophytes from Sicilian grapevine germplasm 

Abstract

The recent expansion of arid areas due to climate change is putting grapevine and the other traditional productions at risk in all Mediterranean countries with a limited availability of fundamental resources such as water. It is possible to improve the resilience of vineyards by developing sustainable agricultural practices based on biological and natural resources such as endophytic microorganisms that colonize inner plant tissues, and which can potentially increase the tolerance to abiotic stresses. A selection of grapevine endophytes was conducted from 2021 to 2023 as part of the PRIMA project PROSIT. In particular, the research aimed to select consortia of endophytic bacteria and/or fungi able to improve the grapevine tolerance to drought. To this aim, vine leaves were harvested during two vegetative seasons, from local varieties subjected to a long-lasting adaptation to arid conditions without regular irrigation. A wide diversity of genera and species belonging to different microbial phyla was isolated on artificial media (NA and PDA) from surface-sterilized grapevine leaves. Selected fungal and bacterial isolates were identified by molecular barcoding based on ITS and 16S rDNA sequences, respectively. Overall, bacterial endophytes were isolated in higher numbers than fungal ones. In 2022, a higher abundance of fungal colonies was isolated at the end of the growing season, suggesting a seasonal dynamic of the microbiota composition. Some of the selected isolates belong to species already known as PGP endophytes of crops, including grapevine. Preliminary in vitro experiments confirmed that those strains can increase the concentration of auxins, ammonium and soluble phosphate.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Dalila Crucitti1*, Irene Doro2, Michela Zottini2, Alessandra Tondello3, Andrea Squartini3, Roberto De Michele1, Francesco Carimi1, Davide Pacifico1

1 IBBR CNR – Institute of Biosciences and Bioresources, via Ugo La Malfa 153, 90146 Palermo
2 Department of Biology, Università degli Studi di Padova, via U. Bassi 58b, 35131 Padova, Italy
3 Department of Agronomy, Animals, Food, Natural Resources, and Environment, DAFNAE Università degli Studi di Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy

Contact the author*

Keywords

drought stress, culturable endophytes, bacteria, fungi

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Contaminations croisées avec les produits phytosanitaires dans les vins bio. Sources potentielles et mesures de prévention.

Organic wines, although resulting from a production method based on the non-use of synthetic phytosanitary products, are not always free of residues. These residues can result from cross-contamination during production in the field or in the cellar, during the production or aging of the wine. In recent years, with the improvement of analysis techniques, a molecule, phosphonic acid, the main metabolite of fosetyl-al (banned in organic farming) is regularly quantified in organic wines and its origin is not clearly identified.

Cover crops under-vine impact on grapevine performance and vineyard soil microorganisms is highly affected by edaphoclimatic conditions at a regional scale 

Soil management through cover crops can influence the cycle of nutrients, promote water infiltration, decrease erosion, and enhance the soil microbiota biodiversity, improving the grapevine performance. However, the area under the vines tends to be left bare by applying herbicides or tillage to avoid competition with the crop in semi-arid climates. Use of covers under-vine might be an alternative to these practices aiming at grapevine quality and soil health improvement. The aim of this research was to study the implications of soil management under the vines (cultivation and cover crops) on growth, yield, berry composition and soil microbial communities. A cover crop composed by a mixture of legumes was sown and compared with a control (cultivation), which includes frequent tillage to keep the soil bare, in three areas characterized by different edaphoclimatic conditions in the region of Navarra.

The dynamics of δ13C and δ18O in musts during berries development

Aim: Many processes or reactions that occur in plants involved isotopic discrimination. Water availability, for example, affects the isotopic ratio of carbon (δ13C) and oxygen (δ18O). In viticulture, δ13C is used in experiments related to water relations and irrigation in vineyards. δ18O is used much less but it could be a good complement to δ13C. The aim of this study was to generate knowledge on how these isotopic ratios, measured in musts, could help to better understand the water behavior of grape varieties. 

Study to optimize the effectiveness of copper treatments for low impact viticulture

Among all pathologies that afflict grapevine, Downy Mildew (DM) is the most important. Generally controlled using Copper (Cu), recently European Commission confirmed its usage but limiting the maximum amount to 28 Kg per hectare in 7 years (Reg. EU 2018/1981).

Can wine competition awarded points be correlated with wine chromatic and aromatic composition?

The quality of wine is difficult to define. This is most certainly accredited to everyone´s different perception of quality. Some of the indicators of high-quality wines are complexity, balance, color and intensity. Color is one of the most crucial attributes of quality, not only for the obvious implications for their perception but also because they are indicators of other aspects related to its aroma and taste. Phenolic compounds are the main responsible for wine color, being anthocyanin and tannins the most determinant compounds in red wines. In addition to color, wine aroma is another important attribute linked with quality and consumer preferences.