terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - New biotechnological tools 9 Deciphering the function and regulation of VviEPFL9 paralogs to modulate stomatal density in grapevine through New Genomic Techniques

Deciphering the function and regulation of VviEPFL9 paralogs to modulate stomatal density in grapevine through New Genomic Techniques

Abstract

Stomata are microscopic pores mainly located in leaf epidermis, allowing gas exchanges between plants and atmosphere. Stomatal initiation relies on the transcription factor SPEECHLESS which is mainly regulated by the MAP kinase cascade, in turn controlled by small signaling peptides, the Epidermal Patterning Factors (EPF and EPF-Like), namely EPF1, EPF2 and EPFL9. While EPF1 and EPF2 induce the inhibition of SPEECHLESS, their antagonist, EPFL9, stabilizes it, leading to stomatal formation. In grapevine, there are two paralogs for EPFL9, VviEPFL9-1 and VviEPFL9-2. Despite their structural similarity, it remains unclear whether they are differentially regulated and have distinct roles. In our study we showed that while VviEPFL9-1 is expressed only in the apex, VviEPFL9-2 is expressed both in the apex and in mature leaves along the plant axis and is significantly repressed by ABA. To support experimental data, an in-silico transcriptomic analysis has been carried out using publicly available datasets. In addition, both genes were functionally characterized using knock-out (KO) mutants generated via CRISPR/Cas9. Leaf stomatal density and gas exchange parameters were significantly different between ‘Sugraone’ WT plants and VviEPFL9-2 KO lines, whereas the differences were negligible between WT and VviEPFL9-1 KO lines. Moreover, a water stress experiment was carried out to deeply compare the physiology of edited lines and WT plants under drought conditions. Our results suggest that VviEPFL9 paralogs have distinct roles in determining stomatal plasticity during leaf growth, and that VviEPFL9-2 may be considered a key target to increase grapevine resilience to water deficiency.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Umar Shahbaz1,2, Pierre Videau3, Katerina Labonova3, David Navarro-Payá4, Alvaro Vidal1,2, José Tomás Matus4, Mickael Malnoy1, Olivier Zekri3, Fabio Fiorani5, Michele Faralli2, Lorenza Dalla Costa1*

1 Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy
2 Center Agriculture Food Environment (C3A), University of Trento, 38098, San Michele all’Adige, Italy
3 Novatech, Mercier Groupe, Le Champ des Noels, France
4 Institute for Integrative Systems Biology, Universitat de València-CSIC,46980 Paterna, Valencia, Spain
5 Institute of Bio- and Geo-Sciences, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany

Contact the author*

Keywords

Vitis vinifera, Epidermal Patterning Factors, CRISPR/Cas9, gas exchange, water stress

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Fleurtai, Soreli and Tocai Friulano: perspectives for quality integration of wine together with protection of the DOCG Lison Classico appellation

In modern viticulture, sustainability must be considered not only into the winery, but in the vineyard as well, being that with the most attentive interventions in order to protect the environment. In this context, the new “fungi resistant” varieties represent a valid option for reducing the negative environmental impact of agrochemicals used in viticulture, including those ones used in organic farming (given the copper accumulation into soils). Several application studies have demonstrated the enological validity of many resistant varieties, both in price and as a blend. Also, under the production point of view, the feasibility and economical sustainability of the new resistant varieties was verified. The aim of this work was to deepen the knowledge on the organoleptic characteristics of wines obtained from the Fleurtai and Soreli varieties and to compare them with the wine obtained from Tocai Friulano, the mother variety in the area destined for the production of the Lison Classico DOCG appellation. The purpose of the work is then to verify the possibility of introducing resistant varieties into the DOCG while maintaining the wine name of the appellation linked to the territory.

A blueprint for managing vine physiological balance at different spatial and temporal scales in Champagne

In Champagne, the vine adaptation to different climatic and technical changes during these last 20 years can be seen through physiological balance disruptions. These disruptions emphasize the general grapevine decline. Since the 2000s, among other nitrogen stress indicators, the must nitrogen has been decreasing. The combination of restricted mineral fertilizers and herbicide use, the growing variability of spring rainfall, the increasing thermal stress as well as the soil type heterogeneity are only a few underlying factors that trigger loss of physiological balance in the vineyards. It is important to weigh and quantify the impact of these factors on the vine. In order to do so, the Comité Champagne uses two key-tools: networking and modelization. The use of quantitative and harmonized ecophysiological indicators is necessary, especially in large spatial scales such as the Champagne appellation. A working group with different professional structures of Champagne has been launched by the Comité Champagne in order to create a common ecophysiology protocol and thus monitor the vine physiology, yearly, around 100 plots, with various cultural practices and types of soil. The use of crop modelling to follow the vine physiological balance within different pedoclimatic conditions enables to understand the present balance but also predict the possible disruptions to come in future climatic scenarios. The physiological references created each year through the working group, benefit the calibration of the STICS model used in Champagne. In return, the model delivers ecophysiology indicators, on a daily scale and can be used on very different types of soils. This study will present the bottom-up method used to give accurate information on the impacts of soil, climate and cultural practices on vine physiology.

Monitoring of ripening and yield of vineyards in Nemea region using UAV

Nemea region is the largest POD zone in Greece. Agiorgitiko (Vitis vinifera L. cv.) is the most cultivated variety in Greece with significant wine potential.

Use of uv light for suppression of grapevine diseases

Microbial pathogens of plant have evolved to sense, interpret, and use light to direct their development. One aspect of this evolved relationship is photolyase-mediated repair of UV-induced damage to pathogen DNA. Application of germicidal UV (UV-C) at night circumvents the blue light-driven repair of pathogen DNA and allows non-phytotoxic doses of UV-C to suppress a variety of pathogenic microbes and even certain arthropod pests without damage to vines or fruit. Lamps arrays have been designed specifically for the canopy architecture of grapevines and have been deployed on both tractor-drawn and robotic carriages for partial to near-complete suppression of powdery mildew (Erysiphe necator), sour rot (fungal, bacterial, and arthropod complex), and downy mildew (Plasmopara viticola).

Feminin vs masculin: the sensorial opposition between chambolle-musigny and gevrey-chambertin wines and the socioeconomical construction of a terroir/taste wine paradigm

At least since de XIXth century, wine writers oppose quite often the wines from Gevrey-Chambertin to the wines from Chambolle-Musigny claiming that the former are more “masculine” (full-bodied, powerful tannins, leathery, rustic…) and the later more “feminine” (delicate, elegant, silky, flowery…).