DNA-free editing to improve stress resilience of wine grape genotypes recalcitrant-to-regeneration

Different studies have demonstrated that the application of ultrasounds (US) to crushed grapes improves chromatic characteristics of the wines (1,2), increases their polysaccharide content (3) and some aroma compounds are also favored (4,5)

Context and purpose. Climate change and the demand for reducing inputs, including chemical compounds, present significant challenges for perennial fruit crops like grapes and apples.

The Armenian highlands are located on the northern border of western asia and stretch up to the caucasus from the north. Throughout human history, country has played an important role in connecting the civilizations of europe and the near east. The recent large-scale study about the dual domestication origin and evolution of grapes approved that in the Armenian highlands human and grapevine stories are interlaced through centuries and roots of grapevine domestication are found deep in the pleistocene, ending 11.5 thousand years ago. Findings of this study confirmed that glacial episodes distinguish wild grapes into eastern and western ecotypes around 200-400 ka.

The grapevine is facing significant challenges due to climate change, as rising temperatures impact its physiological traits and disrupt plant phenology.

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.