DNA-free editing to improve stress resilience of wine grape genotypes recalcitrant-to-regeneration

In 1979 an enlightened and farsighted business owner in an area and in an activity unknown to him and in 120 hectares of land cultivated with corn and wheat expressed to one of us that he wanted to start a business in the wine sector. The first innovative “Vigna Dogarina Scientific Applicative Project” has become famous and harmoniously inserted in and with the “Territoir” of eastern Veneto in northeastern Italy. The revolutionary project allowed one of us: 1. to put into practice results of research related to the applied philosophy, vision, methodology of the “Great MetaEthic Chain 4.1C®” algorithm of the “Conegliano Campus 5.1C®” that considers all material, immaterial, spiritual, technical, economic, environmental, social, existential, relational, ethical, MetaEthical factors with basic indexing in a harmonious chain “ 4.1C®” and application “5.1C®”, 2. to implement:

The soil plays a pivotal role in the agroecological transition processes, due to its numerous implications in production support, water regulation, air and nutrient supply, and its function of reservoir for the major part of planet biodiversity. Therefore, soil quality and adequate soil management are key levers for an ecologically and economically sustainable viticulture. Gascogn’Innov (2017-2022) is an Operational Group funded by the European Innovation Partnership for Agriculture. As such, it gathered winegrowers from the south-west of France (Gascony), scientists, advisors and technicians, around a project focused on the biological functioning of viticultural soil and the design of better-adapted technical paths for soil protection.

The paper presents results that develop the results of studies carried out in 2022-2023 under the OIV grant on the topic of distribution of deuterium (2H(D)) in the intracellular water of grapes and wines, taking into account the impact of natural, climatic and technogenic factors using quantitative nuclear magnetic resonance spectroscopy (qNMR).

Polyphenols are present in a wide variety of plants and foods such as tea, cacao and grape1. An important sub-class of these compounds is the flavanols present in grapes and wines as monomers (e.g (+)-catechin or (-)-epicatechin), or polymers also called condensed tannins or proanthocyanidins. They have important antioxidant properties2 but their biosynthesis remains partly unknown. Some recent studies have focused on the role of glycosylated intermediates that are involved in the transport of the monomers and may serve as precursors in the polymerization mechanism3, 4. The global objective of this work is to identify flavanol glycosides in grapes or wines, describe their structure and determine their abundance during grape development and in wine.

Il Barbera rappresenta sicuramente uno dei più importanti vitigni autoctoni del Piemonte occu­pando circa il 50% della superficie vitata regionale. Esso è ancora diffuso su un’area molto vasta, che si estende per oltre 200.000 ha, dando origine a diverse produzioni vinicole tutelate da denominazioni d’origine.