DNA-free editing to improve stress resilience of wine grape genotypes recalcitrant-to-regeneration

The aim of this ongoing study is to evaluate the degree of adaptability of grapevine scion:rootstock combinations to different conditions of water constraint. Here we present results from the young vine development phase, using three scenarios of water constraint that were implemented from planting. The experimental vineyard was established in 2020 and the data presented will cover the 2021/2022 and 2022/2023 seasons. The experiment consisted of the cultivars Pinotage (PIN), Shiraz (SHI) and Cabernet Sauvignon (CAB), grafted on two rootstocks, Richter 110 (R110) and USVIT-8-7 (US87).

Recent advancements in genomic sequencing technologies have enabled more detailed and direct studies of DNA methylation, which can help characterise epigenetic variations in plants. The Grapevine Improvement team at the Bragato Research Institute is studying the use of Oxford Nanopore sequencing to identify epigenetic changes associated with environmental differences among clonally-propagated grapevines.

This study involved sequencing DNA from the same Sauvignon Blanc clone, sourced from diverse New Zealand viticultural regions, using the PromethION platform.

In 2019, we have planted the red variety Manseng Noir, as it has been shown that it is the only sister of the Tannat grape. Tannat was introduced to Uruguay in 1870 from the south-western regions of France.

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.

Grapevines are continually wounded throughout their cultivation especially during winter pruning. Grapevines respond to wounding by occluding xylem vessels with gels or tyloses to limit pathogen attack and dehydration of the tissues. Although the production of xylem vessel occlusions has been studied in grapevine, to date we have no knowledge of whether different genotypes respond differently. The objective of this study was to characterize the genetic variation in xylem vessel occulsions in five different scion genotypes pruned at different dates.