DNA-Free genome editing confers disease resistance in grapevine

The wine industry works to minimize pesticides and adapt to climate change. Breeding programs have developed disease-resistant grape varieties, particularly against downy and powdery mildew, to minimize pesticide applications [1]. However, their enological potential remains underexplored.

Nitrogen (N) fertilizer is important for grape growth and the quality of wine. It is essential to address the mismatch between N application and wine composition. Cabernet Gernischt (Vitis vinifera L.), as one of the main wine-grape cultivars in China, was introduced to Yantai wine region in 1892. This grape cultivar is traditionally used for quality dry red wine with fruit, spices aroma, ruby red and full-bodied wines. In order to regulate vine growth and improve grape and wine quality, Cabernet Gernischt grapevines were subjected to decreased levels of N treatments, compared to normal N supply treatment, during grape growing seasons of 2019 and 2020.

La création officielle du groupe Experts Zonage Vitivinicole à l’O.I.V., qui s’inscrit dans la Commission Viticulture, est récente. Le Professeur Mario FREGONI en assure la présidence depuis 1998, assisté du vice-président et du secrétaire général Mario FALCETTI. Ils ont été confirmés dans leurs fonctions lors des sessions de mars 2001. Actuellement, le groupe d’experts Zonage Vitivinicole de l’O.I.V. se compose de 40 délégués, représentant 18 pays membres. La mise en place de ce groupe a tout d’abord été initiée par l’Instituto Agrario de San Michele (Italie) et l’Unité de Recherches Vigne et Vin du Centre INRA d’Angers (France). Une collaboration entre les chercheurs s’est installée très tôt, dès 1987.

A new process for vacuum evaporation was developed where evaporation takes place near the inner surface of a vortex produced by a rotor submerged in the liquid. Contrary to the state of the art the Vortex rotor process does not need a vacuum vessel but the rotating liquid creates a geometrically stable low pressure void surrounded by a vortex stabilized by the equilibrium between centrifugal forces and the pressure difference. First tests with water and sugar solutions at concentrations similar to grape must were conducted to verify the theoretical predictions, test the performance under different conditions and study the effect of various process parameters (Rösti et al 2015).

Hanseniaspora vineae (Hv) is a fermenting non-Saccharomyces yeast that compared to Saccharomyces cerevisiae (Sc) present some peculiar features on its metabolism that make it attractive for its use in wine production. Among them, it has been reported a faster yeast lysis and release of polysaccharides, as well as increased ß-glucosidase activity. Hv also produces distinctive aroma compounds, including elevated levels of fermentative compounds such as ß-phenylethyl acetate and norisoprenoids like safranal. However, it is known for its high nutritional requirements, resulting in prolonged and sluggish fermentations, even when complemented with Sc strain and nutrients.