DNA-Free genome editing confers disease resistance in grapevine

Grapevine downy mildew (GDM), caused by the oomycete Plasmopara viticola, is one of the most destructive diseases of Vitis vinifera worldwide. All V. vinifera cultivars are susceptible to P. viticola infection, and epidemics can spread across an entire vineyard within a matter of weeks. Severe outbreaks cause substantial reductions in yield and fruit quality. Tracking GDM spread by manual scouting is time-consuming and unfeasible over large spatial extents.

Extension of the growing season, compression of the annual growth cycle and higher frequency and severity of weather extreme events are consistent features of global warming. While mitigation of factors causing global warming is necessary in the medium-long term, wine growers need “ready to go” adaptation practices to counteract negative effects bound to climate change. This must be done in a sustainably way, meaning that remunerative yield, desired grape quality, low production cost and environment friendly solutions must be effectively merged. In this work, we will review contribution given over the last two decades prioritizing issues related to scion and rootstock choice, changes in vineyard floor management, new perception related to the use of irrigation in vineyards, adaptation practices aimed at decompress maturity, solutions to counteract or minimize damages due to late frost and sunburn and, lastly, some hints on how precision viticulture can help with all of this.

Current remote sensing strategies rely heavily on reflectance data and energy balance modelling using thermal imagery to estimate crop water use and stress. These approaches show great promise for driving precision management decisions, but still require work to better understand how detected changes relate to meaningful physiological changes. Under water stress, grapevines exhibit a range of responses involving both biological and physical changes within leaves and canopies.

In a climate change context, the management of Mediterranean vineyards should be adapted to the new environmental conditions. Predictive models underline that in the future the most of the Mediterranean vineyard regions is expected to experience further warming events producing challenges in ripening balanced fruit. It is already registered that in warm and dry summers, the ripening process is faster and the balance between phenolic and technological (sugar) maturity may not be the desirable. This study investigates the use of specific inactivated yeast derivatives sprayed on the entire canopies of field grown cv Muscat Hamburg vines.

Intensification is considered one of the major drivers of biodiversity loss in farmland. The more intensive management practices that have been adopted the last decades, contributed to species declines from all taxonomic groups. Moreover, agricultural intensification has led to an important change of land use. Complex, mixed agro-ecosystems with cultivated and non-cultivated habitats have been converted to simplified, intensive and homogeneous ones with severe effects on biodiversity.