DNA-Free genome editing confers disease resistance in grapevine

Nitrogen (N) fertilizer is important for grape growth and the quality of wine. It is essential to address the mismatch between N application and wine composition. Cabernet Gernischt (Vitis vinifera L.), as one of the main wine-grape cultivars in China, was introduced to Yantai wine region in 1892. This grape cultivar is traditionally used for quality dry red wine with fruit, spices aroma, ruby red and full-bodied wines. In order to regulate vine growth and improve grape and wine quality, Cabernet Gernischt grapevines were subjected to decreased levels of N treatments, compared to normal N supply treatment, during grape growing seasons of 2019 and 2020.

Grapevine yield and wine quality are dependent on good quality vegetative growth and root development. Soils that restrict proper grapevine root development, together with the high cost of establishing a new vineyard, require effective soil preparation to sustain productive vineyards for 25 years. This study reviews soil preparation research conducted over the past 50 years and identifies best practices to remove soil physical and chemical impediments to create optimum conditions for root growth.

According to the last IPCC report, the scale of recent climate changes are unprecedented over many centuries. Each of the last four decades has been successively warmer than any decade since 1850. Projections for the future foresee that temperature could reach +3.3°C to +5.7°C under the most pessimistic scenario. It is also projected that every region will face more concurrent and multiple changes in climatic impact-drivers. The frequency of extreme climate events is also likely to increase, as well as the occurrence of indirect constraints. These evolving climatic conditions are alrealdy affecting and will continue to affect the suitability of traditional wine grape production areas, but also create opportunities in new locations.

The undergoing global warming scenario is affecting grapevines phenology, including the timing of berry ripening and harvest date, negatively impacting production and quality. This work reports the crucial role of NAC61, a grapevine NAC transcription factor, in regulating metabolic processes occurring from the onset of ripening onwards. NAC61 high confidence targets mainly represent genes acting on stilbene biosynthesis and regulation, and in osmotic and oxidative/biotic stress-related responses. The direct regulation of the stilbene synthase regulator MYB14, the osmotic stress-related gene DHN1b, and the Botrytis cinerea susceptibility gene WRKY52, were all further validated.

The research is part of the “Ecovinegoals” project, financed by Interreg Adrion funds. It aims to encourage the adoption and dissemination of agroecological practices in intensive wine-growing areas. The study focuses on cost analysis of the wine-growing landscape enhancement in an organic winery in order to provide a useful tool for winemakers to direct their investments in green infrastructures. One of the Italian pilot areas of the Ecovinegoals project is the Venezia Biodistrict, characterized by viticulture in a flat reclamation area of 105,800 hectares.