terclim by ICS banner
IVES 9 IVES Conference Series 9 Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Abstract

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed. Three different DNA-based techniques, were used to verify mutations (RAPD, ISSR and SSR markers). We also estimated the ploidy levels of regenerants and differences were observed for 10% of Frappato regenerants that were tetraploid. The different ploidy levels induced several anatomical/morphological changes of the shoots, mature leaves and stomata, which were larger in tetraploid as compared to diploid regenerants. Also, the number of chloroplasts per guard cell pair was higher in tetraploids as compared to diploids; on the contrary, the stomatal index was lower in tetraploids than in diploids. These profound morphological alterations may influence a wide range of physiological processes related to adaptation to environmental stresses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Loredana Abbate1, Caterina Catalano1,2, Antonio Motisi1, Dalila Crucitti1, Francesco Carimi1 and Angela Carra1*

1 Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche Via Ugo La Malfa 153, 90146 Palermo, Italy
2 Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Alfonso Corti 12, 20133 Milan, Italy

Contact the author*

Keywords

autopolyploidy, ploidy variability, somatic embryogenesis,  stomatal characteristics, grapevine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Clustering wine aromatic composition of Vitis vinifera grapevine varieties

Climate change is likely to impact wine typicity across the globe, raising concerns in wine regions historically renowned for the quality of their terroir. Amongst several changes in viticultural practices, replacing some of the planting material (i.e clones, rootstocks and cultivars) is thought to be one of the most promising potential levers to be used for adapting to climate change. But the change of cultivars also involves the issue of protecting the region’s wine typicity.

Interpreting wine aroma: from aroma volatiles to the aromatic perception

Wine contains so many odorants that all its olfaction-related perceptions are, inevitably, the result of the interaction between many odorants.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

The grapevine single-berry clock, practical tools and outcomes 

The dynamic sequence of physiological events along the three-months of berry development from anthesis to ripe stage has been thoroughly investigated. Most studies were performed on average samples, taking care to crush enough fruits to fairly represent the overall trend of the future harvest. However, phenological stages like 30% caps off (EL25) highlights the asynchronous nature of this population. Consequently, softening, onset of sugar accumulation and coloration were melted by asynchrony in a developmental mumbo jumbo, until their respective timing could be clarified by single berries approaches.

Developing and assessing different cordon establishment techniques for long-term vineyard management

Aim: The aim of this research is to quantify the impacts of different cordon establishment techniques on vine health and longevity. It is hypothesised that wrapping developing cordon arms tightly around the cordon wire will cause a constriction of the vascular system, becoming worse over time and disrupting the flow of water and nutrients.