terclim by ICS banner
IVES 9 IVES Conference Series 9 Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Abstract

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed. Three different DNA-based techniques, were used to verify mutations (RAPD, ISSR and SSR markers). We also estimated the ploidy levels of regenerants and differences were observed for 10% of Frappato regenerants that were tetraploid. The different ploidy levels induced several anatomical/morphological changes of the shoots, mature leaves and stomata, which were larger in tetraploid as compared to diploid regenerants. Also, the number of chloroplasts per guard cell pair was higher in tetraploids as compared to diploids; on the contrary, the stomatal index was lower in tetraploids than in diploids. These profound morphological alterations may influence a wide range of physiological processes related to adaptation to environmental stresses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Loredana Abbate1, Caterina Catalano1,2, Antonio Motisi1, Dalila Crucitti1, Francesco Carimi1 and Angela Carra1*

1 Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche Via Ugo La Malfa 153, 90146 Palermo, Italy
2 Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Alfonso Corti 12, 20133 Milan, Italy

Contact the author*

Keywords

autopolyploidy, ploidy variability, somatic embryogenesis,  stomatal characteristics, grapevine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Contaminations croisées avec les produits phytosanitaires dans les vins bio. Sources potentielles et mesures de prévention.

Organic wines, although resulting from a production method based on the non-use of synthetic phytosanitary products, are not always free of residues. These residues can result from cross-contamination during production in the field or in the cellar, during the production or aging of the wine. In recent years, with the improvement of analysis techniques, a molecule, phosphonic acid, the main metabolite of fosetyl-al (banned in organic farming) is regularly quantified in organic wines and its origin is not clearly identified.

An Ag+ SPE method combined with Deans’ switch heart-cutting MDGC–MS/Olfactometry approach for identifying unknown volatile thiols in wine

Wine aroma is a crucial quality criterion. A multitude of volatile compounds have been identified and correlated to the aroma attributes perceived in wine.

Vine selection in France: An assessment after more than 60 years of work

It was at the end of the second world war that professor Branas laid the foundations of french vine selection. He was also behind the creation of domaine de vassal (1949) and antav (1962), which were to become the bridgeheads of the french strategy for the conservation, selection and multiplication of viticultural diversity. Initially based on visually virus-symptom-free massal selections, with the main aim of providing healthy, clearly-identified plant material, the process evolved as knowledge gained towards clonal selection.

Old but gold: the interest for minor grapevine varieties – a case study in the Veneto region (Italy)

The biodiversity of cultivated vines has been significantly reduced due to a series of factors that have favoured the cultivation of a limited number of varieties and clones over time. In veneto, since 1980, a series of important actions have been implemented to counter this process. These actions have focused on the conservation of germplasm identified in the territory and the recovery of varieties historically present in the region, which were in danger of being abandoned and disappearing.

Relationship between soil and grapevine variety in the wineyard of Jura: example for the “Trousseau” variety from the “Terroir” of Montigny-Lès-Arsures (France)

Seven plots located in the commune of Montigny-lès-Arsures (Jura, 39), planted with grapevine varieties Trousseau and Savagnin, were chosen for a study of soil pits and a distribution of major and trace chemical elements in soils and wines. It was shown that the mineral matrix of the soil reflects the geological substratum and the sub-surface alteration process, while the organic soil matrix depends on agro-viticultural practices.