terclim by ICS banner
IVES 9 IVES Conference Series 9 Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Somatic embryogenesis and polyploidy in grapevine: morphological shoot and leaf traits variations

Abstract

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed. Three different DNA-based techniques, were used to verify mutations (RAPD, ISSR and SSR markers). We also estimated the ploidy levels of regenerants and differences were observed for 10% of Frappato regenerants that were tetraploid. The different ploidy levels induced several anatomical/morphological changes of the shoots, mature leaves and stomata, which were larger in tetraploid as compared to diploid regenerants. Also, the number of chloroplasts per guard cell pair was higher in tetraploids as compared to diploids; on the contrary, the stomatal index was lower in tetraploids than in diploids. These profound morphological alterations may influence a wide range of physiological processes related to adaptation to environmental stresses.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Loredana Abbate1, Caterina Catalano1,2, Antonio Motisi1, Dalila Crucitti1, Francesco Carimi1 and Angela Carra1*

1 Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche Via Ugo La Malfa 153, 90146 Palermo, Italy
2 Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Alfonso Corti 12, 20133 Milan, Italy

Contact the author*

Keywords

autopolyploidy, ploidy variability, somatic embryogenesis,  stomatal characteristics, grapevine

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Deciphering the color of rosé wines using polyphenol targeted metabolomics

The color of rosés wines is extremely diverse and a key element in their marketing. It is due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making.

IDENTIFYING POTENTIAL CHEMICAL MARKERS RESPONSIBLE FOR THE PERMISSIVENESS OF BORDEAUX RED WINES AGAINST BRETTANOMYCES BRUXELLENSIS USING UNTARGETED METABOLOMICS

All along the red winemaking process, many microorganisms develop in wine, some being beneficial and essential, others being feared spoilers. One of the most feared microbial enemy of wine all around the world is Brettanomyces bruxellensis. Indeed, in red wines, this yeast produces volatile phenols, molecules associated with a flavor described as “horse sweat”, “burnt plastic” or “leather”. To produce significant and detectable concentrations of these undesired molecules, the yeasts should first grow and become numerous enough. Even if the genetic group of the strain present and the cellar temperature may modulate the yeast growth rate¹ and thus the risk of spoilage, the main factor seems to be the wines themselves, some being much more permissive to B. bruxellensis development than others.

Effect of soil texture on early bud burst

Notre objectif est d’étudier de façon précise les relations entre la physiologie de la vigne et le sol, en prenant en compte l’effet millésime. Nous avons plus précisément étudier la précocité de débourrement de la vigne (stade D) en fonction de la texture du sol et plus particulièrement de la teneur en éléments grossiers.

Relation between the environmental factors of the terroir system and flavan-3-ol composition of grape berry seeds and skin at pre-veraison stage and harvest Influence of dedicate viticultural management

Quantity and quality of flavonoïds in grape berries are important parts of their global quality. Several studies had shown that tannins are responsible for some major flavour properties of red wines such as colour, bitterness and astringency. Nevertheless, their synthesis and properties are still misunderstood. Some studies had suggested that the tannic pool was set before veraison. Thus, the comprehension of the relations between environment and setting of this tannic pool, up to the harvest, is not sufficient.

Climate and mesoclimate zonification in the Miño valley (Galicia, NW Spain)

Galicia est une région située dans le Nord-Ouest de l’Espagne avec une longe tradition de culture de la vigne. A jour d’oui la vigne occupe en Galicia presque 28.500 ha, desquelles 8.100 correspondent aux 5 zones ayant droit à l’appellation DO (« Denominación de Origen ») équivalent aux AOC françaises.