Enhancing plant defense: carbon dots for efficient spray-induced gene silencing 

Although some wine production processes still rely on post-production evaluation and off-site laboratory analysis, the new winemaking industry is aware of a need for a better knowledge of the process to improve the properties of the final product. Thus, more and more wineries are interested in incorporating quality-by-design (QbD) strategies instead of postproduction testing because of the possibility to early detect deviations in fermentation or any other wine process. This would allow to detect unwanted situations and eventually to ‘readjust’ the process, thus minimizing rejects.

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The studies developed by INRA and UV, in Angers, concern wine-growing areas and their optimized management, both from an agro-viticultural and oenological point of view. Previous work (Morlat, 1989) made it possible to give a scientific dimension to the concept of viticultural terroir and demonstrated the considerable influence of this production factor on the quality and typicity of wines (Asselin et al, 1992 ) . A methodology for the integrated characterization of terroirs, based on the “Basic Terroir Natural Unit” (considered as the smallest spatial unit of territory usable in practice, and in which the response of the vine is homogeneous), has been development (Riou et al , 1995).

Alcoholic fermentation is the main step for winemaking, mainly performed by the yeast Saccharomyces cerevisiae. But other wine yeasts called non-Saccharomyces may contribute to alcoholic fermentation and modulate the wine aroma complexity. The recurrent problem with the use of these non-Saccharomyces yeasts is their trend to die off prematurely during alcoholic fermentation, leading to a lack of their interesting aromatic properties searched in the desired wine. This phenomenon appears to be mainly due to interactions with S. cerevisiae. These interactions are often negatives but remain unclear because of the species and strain specific response. Among the non-Saccharomyces yeasts, Lachancea thermotolerans is a wine yeast naturally found in grape must and well known as a great L-lactic acid producer and an aromatic molecules enhancer, but its behavior during alcoholic fermentation can be completely different in co-fermentation with S. cerevisiae in function of strain used.

Drought and heat waves deriving from climate change have been affecting grapevine plants and altering wine characteristics in the past years, and effects are expected to get worst. Innovative approaches to address this problem have been undertaken in several varieties, that consist in exploring intravarietal variability to identify genotypes that are tolerant to abiotic stress. Such is the case of the variety Arinto, where an experimental population of 165 clones installed according to a resolvable row-column design with 6 replicates, was scanned for several parameters, including surface leaf temperature (SLT). Linear mixed models were fitted to the data of the traits evaluated, and the empirical best linear unbiased predictors (EBLUPs) of genotypic effects for each trait were obtained as well as the coefficient of genotypic variation (CVG) and broad sense heritability.