Enhancing plant defense: carbon dots for efficient spray-induced gene silencing 

Soil and climate of 3 vineyards have been characterised in order to determine their influence on grape quality. These vineyards are located in Conca de Barberà (Catalonia, NE Spain) and belong to Cabernet sauvignon and Grenache noir cultivars. All 3 plots are very close, so only interannual climatic data of the nearest meteorological station have been considered.

Most of the effects of ultrasound (US) result from the collapse of bubbles due to cavitation. The shockwave produced is associated with shear forces, along with high localised temperatures and pressures. However, the high-speed stream, radical species formation, and heat generated during sonication may also affect the stability of some enzymes and proteins, depending on their chemical structure. Recently, Ce-lotti et al. (2021) reported the effects of US on protein stability in wines. To investigate this further, the effect of temperature (40°C and 70°C; 60s), sonication (20 kHz and 100 % amplitude, for 20s and 60s, leading to the same temperatures as above, respectively), in combination with Aspergillopepsins I (AP-I) supplementation (100 μg/L), was studied on unstable protein concentration (TLPs and chitinases) using HPLC with an UV–Vis detector in a TLPs-supplemented model system and in an unstable white wine.

Aims: The aims of this study are to determine the influences of the local geology, mineralogy and geochemistry of surroundings, substrate and soil on the cultivation of vines, these as an additional factor of specificity and locality in the production of wine and definition of terroir, as well as for the discrimination of local variance of substrate and soil properties for the strategic management of cultivation plots and/or the evaluation of new cultivation regions, necessary within a scope of global climate change.

Increasing soil organic carbon (SOC) in vineyards enhances soil health with associated benefits for climate change resilience and mitigation.

Grapevine is subject to multiple stresses, either biotic or abiotic, frequently in combination. These stresses may negatively impact the health status of plants and reduce yields. For biotic stress, grapevine is affected by numerous pest and diseases such as downy and powdery mildews, grey mold, black rot, grapevine fanleaf virus and trunk diseases (namely GTDs). The interaction between grapevine and pathogens is relatively complex and linked to various pathogenicity factors including cell-wall-degrading enzymes (especially CAZymes) and phytotoxic secondary metabolites, growth regulators, effectors proteins, and fungal viruses.