terclim by ICS banner
IVES 9 IVES Conference Series 9 Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Abstract

Grapevine (Vitis spp.) is a globally significant fruit crop, and enhancing its agronomic and oenological traits is crucial to meet changing agricultural conditions and consumer demands. Conventional breeding has played a key role in domesticating grapevine varieties, but it is a time-consuming process to develop new cultivars with desirable traits for cultivation.
New plant breeding techniques (NpBTs) offer a potential revolution in grapevine cultivation, and genome editing has shown promise for targeted mutagenesis. The success of these biotechnological approaches relies on efficient in vitroregeneration protocols, particularly through somatic embryogenesis (SE). This method has proven successful in some Vitis species, but its effectiveness varies due to the genotype-dependent nature of many cultivars. Moreover, protoplasts have proven to be particularly suitable for genome editing applications, but protoplasts regeneration remains generally considered inefficient in grapevine.
The focus of this study is to enhance in vitro plant regeneration protocols via SE and isolate and regenerate plants from protoplasts derived from embryogenic calli of the Corvina veronese, a variety economically important in Veneto region. Protoplasts will serve as a platform for DNA-free genome editing using CRISPR/Cas9 to target genes responsible for grapevine susceptibility to powdery and downy mildew. The study includes a preliminary phenotypic characterization of regenerated plants to assess whether gene editing or the regeneration process has influenced their morphology and behaviour compared to plants grown under standard conditions. This research aims to accelerate the development of grapevine varieties with improved traits, addressing the challenges posed by conventional breeding methods.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clarissa Ciffolillo1*, Edoardo Bertini2, Stefania Zattoni1, Sara Lissandrini1, Sara Zenoni1, Giovanni Battista Tornielli1,3

1Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2Edivite s.r.l. San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova, Italy
3Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

Vitis vinifera, Corvina veronese, Somatic embryogenesis, Protoplasts, DNA-free genome editing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A applied viticultural zoning, based on the “secteurs de la reference” methodology, in the Cognac vineyard (France)

Dans les Charentes, en réponse à une crise de production du vignoble destiné à la production de Cognac, un plan de diversification viticole pour des vins de pays de qualité est mis en place. Il nécessite une connaissance des sols et de leurs caractéristiques viticoles pour orienter le choix des types de vins et adapter l’itinéraire technique de production.

Comparative study of qualitative and quantitative characters of grape cultivar ‘Mavrodafni’ (Vitis vinifera L.) grown in different regions of the PDO Mavrodafni Patras

‘Mavrodafni’ (Vitis vinifera L.) is considered one of the oldest grapevine cultivars indigenous to the Greek vineyard, with western Peloponnese being its primary center of cultivation. ‘Renio’ is considered to be either a variant of ‘Mavrodafni’ or an altogether different cultivar. Both ‘Mavrodafni’ and ‘Renio’ can be found in the vineyards of the centers of cultivation, since ‘Renio’ is considered to be more productive compared to ‘Mavrodafni’, and for this reason, it has gradually replaced ‘Mavrodafni’ from cultivation over the course of time. The aim of the present study was to assay the mechanical properties, the polyphenolic content and the antioxidant capacity of skin extracts and must of berries coming from ‘Mavrodafni’ and ‘Renio’, cultivated in the same vineyard as well as in the different regions of cultivation of the PDO Mavrodafni Patras.

Identification of cis-2-methyl-4-propyl-1,3-oxathiane as a new volatile sulfur compound (VSC) in wine

Despite their trace concentrations, volatile sulfur compounds (VSCs) are an important category of flavour-active compounds that significantly contribute to desirable or undesirable aromas of many foods and beverages. In wines, VSCs in the form of polyfunctional thiols, notably 3-sulfanylhexan-1-ol (3-SH), 3-sulfanylhexyl acetate (3-SHA), and 4-sulfanyl-4-methyl-pentan-2-one (4-MSP), possess extremely low olfactory thresholds (≈ ng/L) and pleasant “tropical aroma” notes. They have received much attention with respect to their sensory contributions, quantitative occurrences, biogenesis, and thiol management through viticulture and winemaking. However, the fate of these potent volatiles are still not fully understood.

Research summary on the use of Terroir as a wine purchasing cue

Due to the current challenging nature of the global wine market, and recent growth in number and strength of competitors from non-traditional wine producing countries, European wine producers are focussing on the potential to develop a competitive advantage through the concept of terroir.

Wine tartaric stability based on hydrogel application

Tartrates are salts of tartaric acid that occur naturally in wine and lead to sediments that cause consumers’ rejection. There are currently different treatments to prevent its occurrence, with cold stabilization being the most traditional and well-known method.