terclim by ICS banner
IVES 9 IVES Conference Series 9 Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Abstract

Grapevine (Vitis spp.) is a globally significant fruit crop, and enhancing its agronomic and oenological traits is crucial to meet changing agricultural conditions and consumer demands. Conventional breeding has played a key role in domesticating grapevine varieties, but it is a time-consuming process to develop new cultivars with desirable traits for cultivation.
New plant breeding techniques (NpBTs) offer a potential revolution in grapevine cultivation, and genome editing has shown promise for targeted mutagenesis. The success of these biotechnological approaches relies on efficient in vitroregeneration protocols, particularly through somatic embryogenesis (SE). This method has proven successful in some Vitis species, but its effectiveness varies due to the genotype-dependent nature of many cultivars. Moreover, protoplasts have proven to be particularly suitable for genome editing applications, but protoplasts regeneration remains generally considered inefficient in grapevine.
The focus of this study is to enhance in vitro plant regeneration protocols via SE and isolate and regenerate plants from protoplasts derived from embryogenic calli of the Corvina veronese, a variety economically important in Veneto region. Protoplasts will serve as a platform for DNA-free genome editing using CRISPR/Cas9 to target genes responsible for grapevine susceptibility to powdery and downy mildew. The study includes a preliminary phenotypic characterization of regenerated plants to assess whether gene editing or the regeneration process has influenced their morphology and behaviour compared to plants grown under standard conditions. This research aims to accelerate the development of grapevine varieties with improved traits, addressing the challenges posed by conventional breeding methods.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clarissa Ciffolillo1*, Edoardo Bertini2, Stefania Zattoni1, Sara Lissandrini1, Sara Zenoni1, Giovanni Battista Tornielli1,3

1Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2Edivite s.r.l. San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova, Italy
3Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

Vitis vinifera, Corvina veronese, Somatic embryogenesis, Protoplasts, DNA-free genome editing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Determination of quality related polyphenols in chilean wines by absorbance-transmission and fluorescence excitation emission matrix (a-teem) analyses

Phenolic composition is essential to wine quality (Cleary et al., 2015; Bindon et al., 2020; Niimi et al., 2020) and its assessment is a strong industrial need to quality management.

ASSESSING THE ROLE OF 27 KNOWN BITTER COMPOUNDS IN COMMERCIAL WHITE WINES COMBINING LC-MS QUANTIFICATION AND SENSORY ANALYSIS

The balance between the different flavours of a wine largely determines its perception and appreciation by the consumers. In white wines, sweetness and sourness are usually the two poles balancing the taste properties. The bitter flavour, on the other hand, is frequently associated with a loss of equilibrium and all white wines (dry and sweet, young and aged) are affected.
Several bitter compounds are already well-described in wines.

Exploring the potential of Hanseniaspora vineae for quality wines production

Traditionally, non-saccharomyces yeasts were deemed undesirable in winemaking, for this reason, it is a common practice to add sulphites to prevent their proliferation during the initial stages of vinification. However, the current research on yeast diversity has unveiled numerous non-saccharomyces strains possessing advantageous traits that enrich the sensory profile of wines. The genus hanseniaspora is often associated with wine fermentation and is also commonly found on grapes.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

ReGenWine: A transdisciplinary project to assess concepts in regenerative viticulture

Regenerative agriculture is a set of agricultural practices that focus on improving the health of the soil, increasing biodiversity, and enhancing ecosystem services.