terclim by ICS banner
IVES 9 IVES Conference Series 9 Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Abstract

Grapevine (Vitis spp.) is a globally significant fruit crop, and enhancing its agronomic and oenological traits is crucial to meet changing agricultural conditions and consumer demands. Conventional breeding has played a key role in domesticating grapevine varieties, but it is a time-consuming process to develop new cultivars with desirable traits for cultivation.
New plant breeding techniques (NpBTs) offer a potential revolution in grapevine cultivation, and genome editing has shown promise for targeted mutagenesis. The success of these biotechnological approaches relies on efficient in vitroregeneration protocols, particularly through somatic embryogenesis (SE). This method has proven successful in some Vitis species, but its effectiveness varies due to the genotype-dependent nature of many cultivars. Moreover, protoplasts have proven to be particularly suitable for genome editing applications, but protoplasts regeneration remains generally considered inefficient in grapevine.
The focus of this study is to enhance in vitro plant regeneration protocols via SE and isolate and regenerate plants from protoplasts derived from embryogenic calli of the Corvina veronese, a variety economically important in Veneto region. Protoplasts will serve as a platform for DNA-free genome editing using CRISPR/Cas9 to target genes responsible for grapevine susceptibility to powdery and downy mildew. The study includes a preliminary phenotypic characterization of regenerated plants to assess whether gene editing or the regeneration process has influenced their morphology and behaviour compared to plants grown under standard conditions. This research aims to accelerate the development of grapevine varieties with improved traits, addressing the challenges posed by conventional breeding methods.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clarissa Ciffolillo1*, Edoardo Bertini2, Stefania Zattoni1, Sara Lissandrini1, Sara Zenoni1, Giovanni Battista Tornielli1,3

1Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2Edivite s.r.l. San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova, Italy
3Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

Vitis vinifera, Corvina veronese, Somatic embryogenesis, Protoplasts, DNA-free genome editing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.

Estudio de la fertilidad de los suelos para la zonificación vitícola de la D.O. MONTILLA-MORILES

La D.O. Montilla-Moriles, situada en el sur de la provincia de Córdoba, corresponde a una de las zonas de mayor interés dentro de la vitivinicultura andaluza. Las formaciones de suelos

Phenolic composition and physicochemical analysis of wines made with the Syrah grape under double pruning in the Brazilian high-altitude Cerrado

This study explores the growing potential of vitiviniculture in Brazil’s Federal District, an emerging wine region marked by unique climatic conditions and innovative cultivation techniques.

Soil proximal sensing provides direction in delineating plant water status of ‘crimson seedless’ (Vitis vinifera L.) vineyards

Crimson Seedless’ (Vitis vinifera L.) is a late-ripening, red seedless table grape cultivar with inadequate anthocyanin accumulation and less than ideal berry size issues

The South African vineyard landscapes: impact on long term cultural practices

This paper follows the one presented by Saayman at the International Symposium on Landscapes of Vines and Wines in the Loire Valley during July 2003. Where Saayman’s paper described the heritage and development of South African vineyard landscapes, this one focuses on how the landscape is used to assist in decision-making concerning the most important long term practices.