terclim by ICS banner
IVES 9 IVES Conference Series 9 Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Abstract

Grapevine (Vitis spp.) is a globally significant fruit crop, and enhancing its agronomic and oenological traits is crucial to meet changing agricultural conditions and consumer demands. Conventional breeding has played a key role in domesticating grapevine varieties, but it is a time-consuming process to develop new cultivars with desirable traits for cultivation.
New plant breeding techniques (NpBTs) offer a potential revolution in grapevine cultivation, and genome editing has shown promise for targeted mutagenesis. The success of these biotechnological approaches relies on efficient in vitroregeneration protocols, particularly through somatic embryogenesis (SE). This method has proven successful in some Vitis species, but its effectiveness varies due to the genotype-dependent nature of many cultivars. Moreover, protoplasts have proven to be particularly suitable for genome editing applications, but protoplasts regeneration remains generally considered inefficient in grapevine.
The focus of this study is to enhance in vitro plant regeneration protocols via SE and isolate and regenerate plants from protoplasts derived from embryogenic calli of the Corvina veronese, a variety economically important in Veneto region. Protoplasts will serve as a platform for DNA-free genome editing using CRISPR/Cas9 to target genes responsible for grapevine susceptibility to powdery and downy mildew. The study includes a preliminary phenotypic characterization of regenerated plants to assess whether gene editing or the regeneration process has influenced their morphology and behaviour compared to plants grown under standard conditions. This research aims to accelerate the development of grapevine varieties with improved traits, addressing the challenges posed by conventional breeding methods.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clarissa Ciffolillo1*, Edoardo Bertini2, Stefania Zattoni1, Sara Lissandrini1, Sara Zenoni1, Giovanni Battista Tornielli1,3

1Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2Edivite s.r.l. San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova, Italy
3Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

Vitis vinifera, Corvina veronese, Somatic embryogenesis, Protoplasts, DNA-free genome editing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

IMPACT OF MANNOPROTEIN N-GLYCOSYL PHOSPHORYLATION AND BRANCHING ON WINE POLYPHENOL INTERACTIONS WITH YEAST CELL WALLS

Yeast cell walls (CWs) may adsorb wine components with a significant impact on wine quality. When dealing with red wines, this adsorption is mainly related to physicochemical interactions between wine polyphenols and cell wall mannoproteins. However, mannoproteins are a heterogeneous family of complex peptidoglycans including long and highly branched N-linked oligosaccharides and short linear O-linked oligosaccharides, resulting in a huge structural diversity.

Comparison between satellite and ground data with UAV-based information to analyse vineyard spatio-temporal variability

Currently, the greatest challenge for vine growers is to improve the yield and quality of grapes by minimizing costs and environmental impacts. This goal can be achieved through a better knowledge of vineyard spatial variability. Traditional platforms such as airborne, satellite and unmanned aerial vehicles (UAVs) solutions are useful investigation tools for vineyard site specific management.

Legacy of land-cover changes on soil erosion and microbiology in Burgundian vineyards

Soils in vineyards are recognized as complex agrosystems whose characteristics reflect complex interactions between natural factors (lithology, climate, slope, biodiversity) and human activities. To date, most of the unknown lies in an incomplete understanding of soil ecosystems, and specifically in the microbial biodiversity even though soil microbiota is involved in many key functions, such as nutrient cycling and carbon sequestration. Soil biological properties are indicative of soil quality. Therefore, understanding how soil communities are related to soil ecosystem functioning is becoming an essential issue for soil strategy conservation. Here, we propose to assess the importance of land-cover history on the present-day microbiological and physico-chemical properties. The studied area was selected in the Burgundian vineyards (Pernand-Vergelesses, Burgundy, France) where land occupation has been reconstructed over the last 40 years. Soil samples were collected in five areas reflecting various land cover history (forest, vineyards, shifting from forest to vineyards). For each area, physico-chemical parameters (pH, C, N, P, grain size) were measured and DNA was extracted to characterize the abundance and diversity of microbial communities. The obtained results show significant differences in the five areas suggesting that present-day microbial molecular biomass and bacterial taxonomic is partly inherited from past land occupation. Over longer period of time, such study of land-uses legacies may help to better assess ecosystem recovery and the impact of management practices for a better soil quality and vineyards sustainability.

New insight the pinking phenomena of white wine

Pinking of white wine is an undesired change potentially occurring over storage, leading to the turning of color from yellow into salmon-red hue.

1H-NMR-based Untargeted Metabolomics to assess the impact of soil type on the chemical composition of Mediterranean red wines

Untargeted metabolomics has proven to be an effective method to study the impact of the terroir on metabolic profile of wines. In this context, the aim of this study was to evaluate the effects of different soil types on the chemical composition of Mediterranean red wines, through 1H-NMR metabolomics combined with chemometrics.Grapes from Nero d’Avola L. red cultivar cultivated on four different soil types were separately vinified to obtain four different red wines.One milliliter of raw wine was analyzed by means of a Bruker Avance II 400 spectrometer operating at 400.15 MHz