Using nanopore skim-sequencing to characterise regional epigenetic variability in New Zealand Sauvignon Blanc

The French “Coteaux du Layon” Appellation of Origin has built its Jarne on the production of sweet white wines. A network of experimental plots, based on the “terroir” concept, was established in 1990; it allows for the follow-up of the overripening behaviour of the grapes in relation with the agroviticultural parameters.

A critical aspect of wine quality from a consumer perspective is the overall impression of wine flavour, which is formed by the interplay of volatile aroma compounds, their precursors, and taste and matrix components. Grapes contribute some potent aroma compounds, together with a large pool of non-volatile precursors (e.g. glycoconjugates and amino acid conjugates). Aroma precursors can break down through chemical hydrolysis reactions, or through the action of yeast or enzymes, significantly changing the aroma profile of a wine during winemaking and storage. In addition, glycoconjugates of monoterpenes, norisoprenoids and volatile phenols, together with sulfur-conjugates in wine, provide a reservoir of additional flavour through the in-mouth release of volatiles which may be perceived retro-nasally.

Chitin is the main structural component of a large number of organisms (i.e., mollusks, insects, crustaceans, fungi, algae), and marine invertebrates including crabs and shrimps. The main derivative of chitin is chitosan (CH), produced by N-deacetylation of chitin in alkaline solutions. Over the past decade, the OIV/OENO 338A/ 2009 resolution approved the addition of allergen-free fungoid CH to must and wine as an adjuvant for microbiological control, prevention of haziness, metals chelation and ochratoxins removal (European Commission. 2011). Despite several studies on application of CH in winemaking, there are still very limited and controversial data on its interaction with acidic components in wine (Colan-gelo et al., 2018; Castro Marin et al., 2021).

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Grapevine leaf removal (LR) in the cluster area is typically done between fruit set and cluster closure to create an unfavorable microclimate for fungal diseases, such as Botrytis cinerea and powdery mildew. Grape growers are now turning their attention to pre-flowering LR, which has additional benefits under certain conditions. When applied before flowering, LR strongly affects fruit set and thus the number of berries per cluster. It is therefore a good yield control tool, replacing time-consuming manual cluster thinning (Poni et al. 2006). It also improves berry structure, that is, skin thickness, skin-to-pulp ratio, and berry composition (total soluble solids, titratable acidity, and polyphenols) (Palliotti et al. 2012; Komm and Moyer 2015). By exacerbating competition for assimilates between reproductive and vegetative organs, pre-flowering LR also poses some risks. Excessive yield loss at the same year’s harvest due to a too low fruit set rate is the main concern: intensive pre-flowering LR (100% of the cluster area) can induce up to 50% yield loss in potted vines (Poni et al. 2005). Other parameters, such as cool climatic conditions during flowering, also affect fruit set rate and make it difficult to predict potential yield at harvest. Repeated and overly intensive preflowering LR can have repercussions over time and induce a decline in bud fruiting and plant vigor (Risco et al. 2014).