Using nanopore skim-sequencing to characterise regional epigenetic variability in New Zealand Sauvignon Blanc

vine plant material is one of the major factors of terroir. The vine numbers over 1,000 species, of which the main cultivated species, Vitis vinifera, includes some 6,000 varieties. For the last forty years, selection has been carried out on these, mainly through clonal selection. However, today, only 300 varieties present one or more clones. A dozen varieties are considered as international. The extreme requirements of selection, in terms of diseases, provoke the elimination of the majority of selected plants. This approach to selection is not thorough because it focuses mainly on elimination of virosis and phytoplasma diseases.

Commercial grapevine propagation relies on the ability of dormant wood material to develop adventitious roots.

Methoxypyrazines (MPs) are readily extracted from grape berry and rachis during fermentation and can impart “green” and “herbaceous” sensory attributes to wine. Irrespective of whether MPs, including 3-isobutyl-2-methoxypyrazine (IBMP), 3-isopropyl-2-methoxypyrazine (IPMP), and 3-sec-butyl-2-methoxypyrazine (SBMP), are extracted from berry or other vine material, techniques for remediation of wine with overpowering sensory characters attributable to MPs suffer from poor specificity or produce undesirable sensory outcomes, meaning that alternative control approaches are needed.

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

The evaluation of the current and future impact of climate change on viticulture requires an integrated view on a complex interacting system within the soil-plant-atmospheric continuum under continuous change. Aside of the globally observed increase in temperature in almost all viticulture regions for at least four decades, we observe several clear trends at the regional level in the ratio of precipitation to potential evapotranspiration. Additionally the recently published 6th assessment report of the ipcc (the physical science basis) shows case-dependent further expected shifts in climate patterns which will have substantial impacts on the way we will conduct viticulture in the decades to come.