Using nanopore skim-sequencing to characterise regional epigenetic variability in New Zealand Sauvignon Blanc

In viticulture, microbial communities – particularly fungi – play a vital role in plant health, disease management, and grape quality.

As greenhouse gas emissions continue to rise, climate projections indicate an increased likelihood of heat waves and drier conditions in canada. these changes pose significant challenges to grapevine cultivation, particularly during critical growth stages such as new plantings. interspecific hybrid grape varieties, developed through different breeding programs that combine vitis vinifera with more robust species like v. riparia and v. labrusca varieties, are often touted for their potential resilience to environmental stress.

SO₂ play a major role in the stability and wine during storage. Nowadays, the reduction of chemical input during red winemaking and especially the removing SO₂ is a growing expectation from the consumers. Winemaking without SO₂ is a big challenge for the winemakers since the lack of SO₂ affects directly the wine chemical evolution such as the phenolic compounds as well as its microbiological stability.

Wine grapes breeding has been concentrating a lot of efforts within the grape research community over the last decade. The quick phenotyping of genotype quality traits including aroma composition remains challenging. Selected Ion Flow Tube Mass Spectrometry (SIFT-MS), a technology first available in 2008 and developing rapidly, could be particularly valuable for this usage. The aims of this study were i) to use SIFT-MS, to analyze the whole volatilome from different grape varieties, ii) to assess the ability of this technology to discriminate varieties according to their grape aroma composition, and iii) to study the stability of SIFT-MS signal over maturation to define a sampling strategy.

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