Using nanopore skim-sequencing to characterise regional epigenetic variability in New Zealand Sauvignon Blanc

Climate change is expected to provoke an increase in the frequency and intensity of drought events and water scarcity that will have detrimental effects on photosynthesis and plant yield. To sustain an appropriate plant yield under sub-optimal conditions, a common practice is the application of high amounts of fertilizers with negative environmental consequences. The present study aims at evaluating the interplay between water and nutrient availability, namely nitrogen (N) and potassium (K), in two grapevine cultivars with a different sensitivity to water shortage stress. Two-year-old Vitis Vinifera cv. Cabernet Sauvignon and Grenache grapevine plants grafted on SO4 rootstock have been transferred in pots under semi-environmental conditions.

Since 1998, the GiESCO (previously named GESCO: Groupe d’Etude des Systèmes de COnduite de la vigne) has provided the scientific community with relevant contributions to the study of terroirs. Here is a synthesis of the main terroir-related fields and the major ideas the GiESCO has developed: Basic Terroir Unit and climate, Vine Ecophysiology and microclimate – moderate drought, Vineyard heterogeneity and new technologies, Viticultural Terroir Unit and canopy management, Terroir – Territory and man.

In a context of climate change and excessive use of agrochemical products, sustainable approaches for environmental and human health such as the use of bio stimulants in viticulture represent a potential option, against abiotic and biotic threats. Bio stimulants are organic compounds, microbes, or a combination of both, that stimulate plant’s vital processes, allowing high yields and good quality products. In vines, may trigger an innate immune response leading to the synthesis of secondary metabolites, key compounds for the organoleptic properties of grapes and wines.

Astringency has been defined by the American Society for Testing Materials as “the complex of sensations due to shrinking, drawing or puckering of the epithelium as a result of exposure to substances such as alums or tannins”. Regarding the importance of astringency in wine consumer acceptance, elucidating the molecular mechanisms underpinning this complex sensation represents an important goal for scientists. Although different mechanisms have been described (Gibbins & Carpenter, 2013), the salivary protein precipitation is still the most accepted theory. According to this, wine astringency perceived in the oral cavity is originally attributed to the interaction and subsequence precipitation of salivary proteins by wine tannins –mainly flavanols–.

In recent years, numerous studies have been carried out at the OIV on oenological tannins, both with regard to oenological properties and methods of characterization. The results of these recent studies have led to the revision of the general monograph and the drafting of four new monographs, one for each of the four chemical classes into which the tannins have been grouped: ellagitannins, gallotannins, procyanidins/prodelphinidins, profisetinidins/prorobinetinins.