New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

Agroecology is an integrated approach that simultaneously applies ecological and social concepts and principles to redesign and manage food and agricultural systems, promoting agroecosystems with the necessary biological, socio-economic, and institutional diversity and alignment to support greater efficiency. Thus, several studies have been carried out at promoting the adoption of more agroecological practices among farmers and a wider audience concerning soil conservation and health maintenance.

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.

The jurist feels like an intruder when talking about terroirs. He looks at the press and tries to understand. We can read there about the cooking festival of May 30, 1996 which “..highlights products whose quality depends on a region”, that Camembert du pays d’Auge is the only one to be protected, I was thinking of camembert from Normandy, that 80% of Greek feta is made in the Netherlands, I thought it was in Denmark, and that the European Community protects geographical indications of IGP origin, probably a new category replacing the indications protected areas (1). I also learned that distributors are asking for more local products because “they come to confuse the cards in the part engaged with the big brands”. Carrefour has its “Terroirs and drawers”, Prisunic its “Vent d’Ouest”, Intermarché “Les bouquets du terroir”, Monoprix “Les terroirs de France” (2), Promodés and its brand “Reflets de France” for the “Continent” hypermarkets (3). At the same time it is asserted that “The term is a mere common noun. Unprotectable and therefore unprotected” (4).

The wine spoilage yeast Brettanomyces bruxellensis can be found at several steps in the winemaking process due to its resistance to multiple stress conditions. Among the resistance strategies, one could be the formation of biofilm, a lifestyle known to enhance persistence of microorganisms. In this study, we propose to characterize biofilm of B. bruxellensis in wine, especially through several microscopic analyses.

During the tasting of a fine, old wine, the aromas generated in the glass are intertwined in an intimate, complex manner, expressing the fragrance of the aging bouquet. This aging bouquet, which develops during bottle storage through a complex transformation process, may result in a broad palette of nuances. Among these, undergrowth, truffle, toasted, spicy, licorice, fresh red- and black-berry fruit and mint descriptors were recently identified as features of its olfactory representation for red Bordeaux wines. Although a targeted chemical approach focusing on volatile sulfur compounds revealed the role played by dimethyl sulfide, 2-furanmethanethiol, and 3-sulfanylhexanol as molecular markers of the typicality of the wine aging bouquet of red Bordeaux wines, its chemical transcription has only partially been elucidated.