terclim by ICS banner
IVES 9 IVES Conference Series 9 New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

Abstract

Nowadays, many policies are being adopted for direct agriculture towards more sustainable approaches. To continue to maintain a high production using fewer fertilizers, pesticides and water resources, agronomic techniques must be combined with biotechnological approaches. In grapevine, the breeding programs are restricted by the fact that it has a highly heterozygous genome, therefore, if on the one hand, we try to improve the characteristics, on the other hand it is necessary to preserve the original genome of the varieties. CRISPR-cas9 system is one of the smartest tools to carry out highly precise genetic modifications leaving the genetic background unchanged. To produce edited DNA-free grapevine plant is necessary to dispose of an efficient delivery system to introduce the preassembled ribonucleoproteins (RNP) and to ensure the subsequent step of regeneration of the edited plant. Protoplasts are the best system for this purpose: they represent a highly regenerative platform accessible to most of transformation techniques. The regeneration is possible through somatic embryogenesis. Grapevine is recalcitrant to the regeneration process leading to a low rate of plant recovery. The aim of this study is to optimise the in vitroregeneration process of Cabernet Sauvignon and Glera varieties to apply a DNA-free genome editing approach to improve agronomical and oenological traits. Here, we also provide preliminary studies on the enhancing effect of the current in the regeneration process through the application of an external electric field and evaluating the expression of fluorescent transcriptional reporters of transcription factors involved in shoot regeneration in A.thaliana.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Stefania Zattoni 1*, Edoardo Bertini 2, Erica D’Incà 2, Maddalena Salvalaio 3, Giovanni Sena 3, Sara Zenoni 1

1 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy
2 Edivite s.r.l, San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova
3 Laboratory of plant morphogenesis, Imperial College London, South Kensington Campus, London SW7 2AZ, UK

Contact the author*

Keywords

Protoplast, Somatic embryogenesis, CRISPR-cas9, in vitro regeneration, Electric field

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sensory significance of aroma carry-over during bottling from aromatized wine-based beverages into regular wine

In 2020 one out of  eight wine bottles were filled with a flavoured wine-based beverage.
Read More

Fertility assessment in Vitis vinifera L., cv. Alvarinho

The Portuguese wine production is characterized by wide yield fluctuations, causing considerable implications in the economic performance of this sector. The possibility of predicting the yield in advance is crucial as it enables preliminary planning and management of the available resources. The present work aims to study and evaluate two different techniques for the assessment of vine fertility. vineyards.

Read More

Bunch placement effects on dehydration kinetics and physico-chemical composition of Nebbiolo grapes

Sforzato di Valtellina DOCG is a special reinforced red wine produced using withered Nebbiolo grapes. The withering process takes place in traditional rooms under natural environmental conditions; it starts immediately after the harvest and ends not before the 1st December of the same year. The process can be performed with different bunch placements that can influence the grapes features.The purpose of the study is to compare the effect on grape physico-chemical parameters for four withering bunch placement systems: hanged clusters (HC), plastic crates (CT), breathable mesh fabric on wooden frames panels (MF), and reed mats (RM). For all the systems studied, the withering length was two months at a temperature between 6 and 19 °C and a relative humidity of 41-88%.

Read More

Comparing the effects of vision, smell and taste in red wine quality judgments by experts: sensory cues, mental imagery and verbal representations as drivers of consensus in the multisensory space

In this study, we evaluated the contributions of vision, smell and taste to red wine quality judgments by expert wine tasters.

Read More

Techniques to study graft union formation in grapevine

Grapevines are grown grafted in most viticultural regions. Grapevine rootstocks are either hybrids or pure species of different American Vitis spp. (particularly V. berlandieri, V. rupestris and V. riparia), which were primarily used to provide root resistance to the insect pest Phylloxera. In addition to Phylloxera resistance, grapevine rootstocks were also selected in relation their resistance to various abiotic stress conditions. Future rootstocks should have the potential to adapt viticulture to climate change without changing the characteristics of the harvested product. However, high grafting success rates are an essential prerequisite to be able to use them with all the varieties. The objective of this work is to develop quantitative techniques to characterize graft union formation in grapevine.

Read More