New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

Climate transition with frequent heat waves and long drought periods threatens grapevine productivity and wine quality in the Mediterranean regions. Microorganisms are known to contribute to plant fitness and to stimulate plant resilience against biotic and abiotic factors. In this work, it was assessed the impact of long-term drought on soil microbiome associated to grapevine in open field in Alentejo, renowned Portuguese wine region. Soil and plant tissues of drought tolerant Syrah cultivar exposed to three irrigation levels (100%- FI, 50%-DI ETc; rain-fed–NI) for 5 years were sampled for two years (2022-2023). Metabarcoding analysis of soil bacteria (16S V4 rRNA) and fungi (ITS sub-region) were integrated with soil physiochemical properties and leaves´ physiological data. Pre-dawn leaf water potential and stomatal conductance confirmed the imposed drought scenarios. Even though, α- and β-diversity of prokaryotic and eukaryotic microbial communities differed more by season than water availability, samples clustered according to soil water content and pH (p<0.05). Fungal communities show higher differences in the structure across treatments than bacteria. In 2023, 16 bacterial against 61 fungal ASVs were significatively different in abundance between NI and FI. Beijerinckiaceae, Bradyrhizobiaceae (Alphaproteobacteria) and Nocardioidaceae, Streptomycetaceae (Actinobacteria) families resulted to be significatively more abundant in NI, while Ascomycota, Basidyomicota and Mortierellomycota are the most important fungal phyla in NI. With culturomics data, this study aims to gather insights into how soil microbiome is remodelled under drought and contribute to select bacterial and fungal taxa with potential to mitigate drought stress in vineyards.

Wine tasting has been shown to provide emotions to tasters (Coste et al. 2018). How will expertise impact this emotional response? Burnham and Skilleås (2012) reported that the cultural, experiential, and aesthetic competencies characterize an expert in wine compared to a novice. Although there is no consensual definition of an aesthetic experience, Burnham and Skilleås (2012) reported that aesthetic appreciation is “disinterested, normative for others and communicable” in comparison to sensory pleasure.

Downy mildew is one of the most important grapevine diseases, caused by the Oomycete Plasmopara viticola. The management of the disease in organic agriculture can require up to 15 copper applications per year. However, copper accumulates in the soil, is phytotoxic and is toxic for organisms living in the soil, its use has been restricted in European Union to maximum 28 kg in 7 years. Therefore, testing of alternatives with equal effectiveness is desirable. Among those, the natural biopolymer chitosan, obtained from crab shells, proved to be effective toward downy mildew in plot experiments. The aim of our trials was to extend chitosan applications in large scale experiments in different years, cultivars and environmental conditions.

Today in the wine industry, a lot of alternatives are available for reducing phytosanitary inputs. Among these, prophylaxis, the use of biocontrol products and the deployment of pathogen-resistant vines are the most promising. eugenol (2-methoxy-4-(2-propenyl)-phenol), a molecule with recognised antifungal properties, can contribute to the last two alternatives. This molecule has been identified as an endogenous compound in the baco blanc hybrid variety used in armagnac pdo, which is at least tolerant to botrytis cinerea.

Shrinking berries are the common symptoms of Berry Shrivel (BS) and Late-Season-Dehydration (LSD) in grapevine cultivars.