terclim by ICS banner
IVES 9 IVES Conference Series 9 New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

Abstract

Nowadays, many policies are being adopted for direct agriculture towards more sustainable approaches. To continue to maintain a high production using fewer fertilizers, pesticides and water resources, agronomic techniques must be combined with biotechnological approaches. In grapevine, the breeding programs are restricted by the fact that it has a highly heterozygous genome, therefore, if on the one hand, we try to improve the characteristics, on the other hand it is necessary to preserve the original genome of the varieties. CRISPR-cas9 system is one of the smartest tools to carry out highly precise genetic modifications leaving the genetic background unchanged. To produce edited DNA-free grapevine plant is necessary to dispose of an efficient delivery system to introduce the preassembled ribonucleoproteins (RNP) and to ensure the subsequent step of regeneration of the edited plant. Protoplasts are the best system for this purpose: they represent a highly regenerative platform accessible to most of transformation techniques. The regeneration is possible through somatic embryogenesis. Grapevine is recalcitrant to the regeneration process leading to a low rate of plant recovery. The aim of this study is to optimise the in vitroregeneration process of Cabernet Sauvignon and Glera varieties to apply a DNA-free genome editing approach to improve agronomical and oenological traits. Here, we also provide preliminary studies on the enhancing effect of the current in the regeneration process through the application of an external electric field and evaluating the expression of fluorescent transcriptional reporters of transcription factors involved in shoot regeneration in A.thaliana.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Stefania Zattoni 1*, Edoardo Bertini 2, Erica D’Incà 2, Maddalena Salvalaio 3, Giovanni Sena 3, Sara Zenoni 1

1 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy
2 Edivite s.r.l, San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova
3 Laboratory of plant morphogenesis, Imperial College London, South Kensington Campus, London SW7 2AZ, UK

Contact the author*

Keywords

Protoplast, Somatic embryogenesis, CRISPR-cas9, in vitro regeneration, Electric field

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Characterization of the Origin Denomination “Ribeira Sacra”

“Ribeira Sacra” is an origin denomination located between the provinces of Lugo and Ourense, in Galicia (northwest of Spain).
Read More

Expanding the biotechnological potential of M. pulcherrima/fructicola clade for wine-related applications

AIM: Strains belonging to M. pulcherrima/fructicola clade are frequently isolated from flowers, fruits and grape musts, and exhibit a broad spectrum of enzymatic activities and antimicrobial potential (Morata et al., 2019; Sipiczki, 2020; Vicente et al. 2020).

Read More

Effect of vine nitrogen status on grape and wine quality: Terroir study in the Vaud vineyard (Switzerland)

This study was conducted on soil-climate-plant relations (terroir) and their impact on grape composition and wine quality in the canton of Vaud by Agroscope Changins-Wädenswil ACW

Read More

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Read More

The modification of cultural practices in grapevine cv. Syrah, does it modify the characteristics of the musts?

The work shows the results of a year of experimentation (2020) in a Syrah variety vineyard in La Roda (Castilla-La Mancha, Spain). The trial approach was on a randomized block design with two factors: Irrigation (I) and Pruning (P).
Irrigation schedules were adjusted to apply amounts close to 1,500 m3/ha. With this provision, 2 different irrigation treatments were proposed: I1) Start of irrigation from pea-sized grape to post-harvest (providing at least 20 % of the total amount of irrigation water to be provided post-harvest); I2) Start of irrigation from pea-sized grape to harvest (usual irrigation practice in the study area). Pruning was proposed with two treatments, one at the end of January (P1), which is pruning on a conventional date; and P2) pruning carried out at the beginning of budding. In total, 4 repetitions were designed with 4 elementary plots, each one of them representing one of the proposed treatments (I1P1; I1P2; I2P1; I2P2). In total, 16 plots were worked on and each elementary plot consisted of 30 strains, distributed in 3 lines.
The productive response was evaluated with the yield results of the harvest harvested at 23 ºBrix. The qualitative response was measured in the musts through the indices of technological (acidity, pH and potassium) and phenolic maturity and aromatic compounds in free and glycosylated fractions. The treatments tested had, in general, an effect on the different variables analyzed.

Read More