In vitro tissue culture as a tool for Croatian grapevine germplasm management

Due to the current challenging nature of the global wine market, and recent growth in number and strength of competitors from non-traditional wine producing countries, European wine producers are focussing on the potential to develop a competitive advantage through the concept of terroir.

The recent development of regulatory genomics has raised increasing interest in plant research since transcriptional regulation of genes plays a pivotal role in many biological processes. By shedding light on the target genes of the various transcription factors (TFs), it is therefore possible to infer the influence they exert on the different molecular mechanisms. In this regard, the attention was focused on WRKYs, a family of TFs almost exclusively found in plant species. In grapevine, WRKYs are involved in several biological processes, playing a key role in berry development, hormonal balance and signalling, biotic and abiotic stresses responses, and secondary metabolites biosynthesis.

New Plant Breeding Techniques (NPBTs) have the potential to revolutionize the genetic improvement of grapevine. However, the practical application of these techniques is limited by several challenges, such as the difficulty in generating embryogenic calluses, maintaining their competence during in vitro cultivation, and regenerating plants without defects. To overcome these challenges, we conducted a study to test the effect of two treatments on callus cultures derived from different grapevine varieties, with and without embryogenic competence. The tested substances were Silver Thiosulphate (STS) an ethylene inhibitor, and Salicylic Acid (SA), an elicitor with different effects depending on the concentration of use beyond the ethylene inhibitor activity.

Defined the new paradigm, the applied philosophy, the methodology, the algorithm of the “Charter for Universal Holistic MetaEthic Sustainability 4.1C17.18”, research has continued to define and write, an
handbook that should be:”Complete Universal Holistic MetaEthics 4.1C of descriptors” of the “Charter for Sustainability Universal Holistic MetaEthic 4.1C17.18” with basic and applicative indexing. In these activities and research we have involved over 3500 Italian and non-Italian people from the research world to simple but educated, enlightened and enlightening citizens and we have analyzed over 180000
entries concerning the descriptors above, which represent the basic “descriptors”.

Climate projections for the future suggest favourable conditions for some wine producing regions, but challenging conditions for others. For instance, temperature increases are likely to shift grapevine phenology, ripening and harvest dates, and potentially affect grape quality and yield.