terclim by ICS banner
IVES 9 IVES Conference Series 9 In vitro tissue culture as a tool for Croatian grapevine germplasm management

In vitro tissue culture as a tool for Croatian grapevine germplasm management

Abstract

In vitro culture makes it possible to carry out specific studies that would not be possible with whole plants grown in the field or in a greenhouse. Cryopreservation allows long-term preservation without metabolic changes in the plant material and cryotherapy can be efficient in virus elimination, which is a major scientific challenge.
The preculture media of cryopreservation protocols were evaluated on three Croatian grape varieties with different antioxidants (salicylic acid, ascorbic acid and glutathione). The highest growth in vitro was achieved on the medium with the addition of glutathione and the lowest with the addition of salicylic acid. Growth in vitro and regeneration after cryopreservation depended on genotype and health status. The cryopreservation protocols tested (as part of cryotherapy) did not result in sufficient regeneration after cryopreservation (10-15%) in the varieties tested.
Virus elimination was tested for economically important viruses by meristem tip culture in 18 Croatian varieties. In vitro regeneration ranged 6.82-53.22%, but virus elimination was achieved in only three cultivars and was very low (23.8%). In addition, two new grapevine viruses (GVG and GBV-1) were tested. The results showed a low percentage of virus elimination (2%) by meristem tip culture in three-month-old tissue cultures.
Preliminary research activities were carried out by micrografting with Croatian grape varieties. The survival rate was good, but regeneration was difficult to achieve. A more detailed study is in progress. Overall, the presented methods of meristem tip culture, cryopreservation and micrografting should be further evaluated for the Croatian grapevine germplasm to enable wider application.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Zvjezdana Marković1*, Željko Andabaka1, Domagoj Stupić1, Iva Šikuten1, Petra Štambuk1, Edi Maletić1, Jasminka Karoglan Kontić Jasminka1, Darko Vončina2, Darko Preiner1

1 University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb
2 University of Zagreb, Faculty of Agriculture, Department of Plant Pathology, Zagreb

Contact the author*

Keywords

Vitis vinifera L., cryopreservation, preculture with antioxidants, virus elimination, meristem culture, regeneration

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

WINE FERMENTATION METABOLITES PRODUCED BY TWO TORULASPORA DELBRUECKII STRAINS ISOLATED FROM OKANAGAN VALLEY, BC, CANADA VINEYARDS

Wine aroma is influenced by various factors, from agricultural practices in the vineyard to the enological choices made by winemakers throughout the vinification process. Spontaneous fermentations have a characteristically deeper complexity of aromas when compared to fermentations that have been inoculated with Saccharomyces (S.) cerevisiae because of the diversity of microflora naturally present on grape skins. Non-Saccharomyces yeast are being extensively studied for their ability to positively contribute to wine aroma and flavour. These yeasts are known to liberate more bound volatile compounds present in grape must than S. cerevisiae through the enzymatic action of β-glucosidases and β-lyases1.

Deep learning based models for grapevine phenology

the phenological evolution is a crucial aspect of grapevine growth and development. Accurate detection of phenological stages can improve vineyard management, leading to better crop yield and quality traits. However, traditional methods of phenological tracking such as on-site observations are time-consuming and labour-intensive. This work proposes a scalable data-driven method to automatically detect key phenological stages of grapevines using satellite data. Our approach applies to vast areas because it solely relies on open and satellite data having global coverage without requiring any in-field data from weather stations or other sensors making the approach extensible to other areas.

The influence of different fertiliser applications and canopy management practices on the potassium content and pH of juice and wine of Vitis vinifera L. cvs. Cabernet-Sauvignon and Cabernet franc

In an attempt to reduce the pH of juice and wine, different fertiliser applications and canopy management practices were evaluated in South Africa in a field trial. Fertiliser treatments entailed no, CaSO4, Ca(OH)2, and MgSO4 fertilisation.

Process for partial or total dealcoholization of wine using a post-fermentation microbiological technique

The dealcoholized wine sector is experiencing strong market growth, driven by increasing consumer demand.

Ability of lactic acid bacterial laccases to degrade biogenic amines and OTA in wine

Two of the most harmful microbial metabolites for human health that can be present in wines and either fermented or raw foods are biogenic amines (BA) and ochratoxine A (OTA). Winemakers are aware of the need to avoid their presence in wine by using different strategies, one of them is the use of enzymes. Some recombinant laccases have been characterized and revealed as potential tools to degrade these toxic compounds in wine[1], specifically biogenic amines[2].