In vitro tissue culture as a tool for Croatian grapevine germplasm management

Planting vineyards in cooler climates has been used over recent years as
a strategy to counter the climatic shifts caused by climate change. A move towards higher altitudes in hilly and mountainous wine regions may provide a solution to deleterious effects that increased ambient temperatures have on wine quality. Until now, the influences of higher altitudes and their climates, as well as their effect on vine growing cycles, still holds a lot of scientific uncertainty. The transnational EU-funded project REBECKA (Interreg V-A IT-AT: ITAT1002, duration: 2017-2019) has the objective to develop a regional valuation method to rate the suitability for viticulture in South Tyrol (Italy) and Carinthia (Austria). Preliminary surveys were performed regarding the effects of altitude on ripening performance of the cultivar Pinot Noir.

The wine industry worldwide faces more and more challenges due to climate change, such as increased dryness in some areas, water stress, sunburn and early harvesting during hot summer temperatures¹. One of the resulting problems for the wine quality might be a higher prevalence of the untypical aging off-flavor (ATA)². A substance, which Rapp and Versini made responsible for ATA, is the 2-aminoace-tophenone (2-AAP)³. 2-AAP in wine causes a naphthalene, wet towels, wet wool, acacia flower or just a soapy note⁴.

The climate has warmed over the past century or more bringing about changes in numerous aspects in both earth and human systems

Current remote sensing strategies rely heavily on reflectance data and energy balance modelling using thermal imagery to estimate crop water use and stress. These approaches show great promise for driving precision management decisions, but still require work to better understand how detected changes relate to meaningful physiological changes. Under water stress, grapevines exhibit a range of responses involving both biological and physical changes within leaves and canopies.

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.