In vitro tissue culture as a tool for Croatian grapevine germplasm management

In oenology, bio-protection consists in adding bacteria, yeasts or a mixture of microorganisms on grape must before fermentation in order to reduce the use of chemical compounds such as sulphites.

Sulphur dioxide has been used for many years for its antimicrobial, antioxidant and antioxydasic properties in winemaking but nowadays, it is a source of controversy. Indeed, consumers are more attentive to the naturalness of their foods and beverages and the legislation is changing to reduce the total SO₂ levels allowed in wines. To limit and replace the doses of sulphur dioxide applied, winemakers can now use bioprotection consisting in live yeast addition as alternative,seems to be promising. This process, lightly used in from the food industry, allows to colonize the environment and limit the development or even eliminate undesirable microorganisms without altering the sensory properties of the product.

Recent regulations concerning alcoholic beverages have prompted producers to revise their product labels to incorporate nutritional information. In this context, qr codes containing such information, known as e-labels, are now being employed on wine labels for the first time.

By the end of this century, up to 90% of traditional viticulture regions in the Mediterranean, including Sicily, are projected to face extinction due to escalating climate challenges such as severe droughts, heatwaves, and unseasonal rains.

Grapevine rootstocks have traditionally been chosen in order to manage scion vigour, soil pests and soil conditions. Riparia Gloire de Montpellier (RGM) has been in use since the turn of the 19th century, over 100 years and still a remarkably stable source of phylloxera (Daktulosphaeria vitifoliae Fitch) resistance. The original source material was probably collected near the Missouri/Mississippi river confluence, a mid-continental but more southerly location in the United States. It has been hypothesized that more northerly selections of V. riparia Michx might improve both fall acclimation rate and depth of the scion, thus mitigating late fall frost and midwinter freeze damage.