terclim by ICS banner
IVES 9 IVES Conference Series 9 In vitro tissue culture as a tool for Croatian grapevine germplasm management

In vitro tissue culture as a tool for Croatian grapevine germplasm management

Abstract

In vitro culture makes it possible to carry out specific studies that would not be possible with whole plants grown in the field or in a greenhouse. Cryopreservation allows long-term preservation without metabolic changes in the plant material and cryotherapy can be efficient in virus elimination, which is a major scientific challenge.
The preculture media of cryopreservation protocols were evaluated on three Croatian grape varieties with different antioxidants (salicylic acid, ascorbic acid and glutathione). The highest growth in vitro was achieved on the medium with the addition of glutathione and the lowest with the addition of salicylic acid. Growth in vitro and regeneration after cryopreservation depended on genotype and health status. The cryopreservation protocols tested (as part of cryotherapy) did not result in sufficient regeneration after cryopreservation (10-15%) in the varieties tested.
Virus elimination was tested for economically important viruses by meristem tip culture in 18 Croatian varieties. In vitro regeneration ranged 6.82-53.22%, but virus elimination was achieved in only three cultivars and was very low (23.8%). In addition, two new grapevine viruses (GVG and GBV-1) were tested. The results showed a low percentage of virus elimination (2%) by meristem tip culture in three-month-old tissue cultures.
Preliminary research activities were carried out by micrografting with Croatian grape varieties. The survival rate was good, but regeneration was difficult to achieve. A more detailed study is in progress. Overall, the presented methods of meristem tip culture, cryopreservation and micrografting should be further evaluated for the Croatian grapevine germplasm to enable wider application.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Zvjezdana Marković1*, Željko Andabaka1, Domagoj Stupić1, Iva Šikuten1, Petra Štambuk1, Edi Maletić1, Jasminka Karoglan Kontić Jasminka1, Darko Vončina2, Darko Preiner1

1 University of Zagreb, Faculty of Agriculture, Department of Viticulture and Enology, Zagreb
2 University of Zagreb, Faculty of Agriculture, Department of Plant Pathology, Zagreb

Contact the author*

Keywords

Vitis vinifera L., cryopreservation, preculture with antioxidants, virus elimination, meristem culture, regeneration

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of dosage sugar-type and ageing on finished sparkling wine composition and development of Maillard reaction-associated compounds

The Maillard reaction (MR) is a non-enzymatic reaction between reducing sugars and amino acids, resulting in the production of volatile and flavour-active compounds.

Read More

Ability of Saccharomyces cerevisiae strains to modulate the aroma of albariño wines

The objective of the present work is to evaluate the impact of three S. cerevisiae strains on the comprehensive aroma profile of Albariño wine along its shelf life.

Read More

Linear sweep voltammetry to classify and characterize the antioxidant properties of tannins

In recent years, numerous studies have been carried out at the OIV on oenological tannins, both with regard to oenological properties and methods of characterization. The results of these recent studies have led to the revision of the general monograph and the drafting of four new monographs, one for each of the four chemical classes into which the tannins have been grouped: ellagitannins, gallotannins, procyanidins/prodelphinidins, profisetinidins/prorobinetinins.

Read More

Impact of urbanization on optimum wine Terroirs in the Bordeaux region sample of one township of the Entre-Deux-Mers Area

L’étude présentée porte sur une commune de l’Entre-Deux-Mers dans le bordelais. Nous caractérisons dans un premier temps les potentialités des sols vis-à-vis de la production de vins rouges de qualité (délimitation de terroirs grâce à la prise en compte des couvertures pédologiques et des caractéristiques morphométriques du terrain : pentes, expositions, convexités. Dans un second temps, nous récapitulons l’évolution historique des occupations des sols depuis 1790 : l’emplacement des vignes est ainsi localisé sur les terroirs respectifs des communes. Enfin, une étude prospective résultant d’enquêtes sur l’utilisation du foncier, situe le devenir prévisible de l’espace étudié (en particulier dans ses composantes viticoles et urbaines).

Read More

The influence of terroir on the quality of wine of the Cahors A.O.C

Dans le but d’améliorer la qualité et la typicité des vins de l’Appellation d’0rigine Contrôlée CAHORS, une étude a été réalisée afin de mettre en évidence l’adéquation Cépage-Terroir- Qualité du vin.
Selon la méthodologie proposée par MORLAT et ASSELIN (1992), neuf unités terroirs ont été déterminées. Sur chacune, des parcelles de référence homogènes quant au matériel végétal Cot ou Malbec ( cépage principal de cette appellation greffé sur S04, et aux méthodes culturales, ont été suivies au niveau agronomique et œnologique (GARCIA et al., 1996).

Read More