In vitro tissue culture as a tool for Croatian grapevine germplasm management

Considering that global warming is changing berry ripening timing and progression, uncovering the molecular mechanisms and identifying key regulators governing berry ripening could provide important tools in maintaining high quality grapes and wine. NAC (NAM/ATAF/CUC) transcription factors represent an interesting family due to their key role in the developmental processes control, such as fruit-ripening-associated genes expression, and in the regulation of multiple stress responses. Between the 74 NAC family members, we selected 12 of them as putative regulators of berry ripening: NAC01, NAC03, NAC05, NAC11, NAC13, NAC17, NAC18, NAC26, NAC33, NAC37, NAC60 and NAC61.

In recent years, “no-low” products seem to become a new worldwide trend. It appears to be a possible answer to the well-known context of climate change, the decline in wine consumption, and the wellness/health trend (“free from” claims, vegan, and so on…) That consumers are looking for. The aim of this study is to provide an overview of the “no-low” products sold in the french market (but not only french products), focusing on the labelling, packaging, and sales presentation of these products.

Context and purpose of the study. Climate change is a major challenge in wine production. It results in erratic weather conditions which may lead to a reduction in grape yield and the subsequent grape and wine quality.

Grapevine cultivation is threatened by the global warming, which combines high temperatures and reduced rainfall, impacting in wine quality and even plant survival. Breeding for varieties resilient to these challenges must address plant traits such as tolerance to supraoptimal temperatures and optimized water use efficiency while minimizing productivity and quality losses. Stomatal abundance (SA) determines the maximum leaf potential for transpiration and thus water loss and cooling. Since SA results from a developmental process during leaf emergence and growth, knowledge on the genetic control of this process would provide specific targets for modification.

The recent expansion of arid areas due to climate change is putting grapevine and the other traditional productions at risk in all Mediterranean countries with a limited availability of fundamental resources such as water. It is possible to improve the resilience of vineyards by developing sustainable agricultural practices based on biological and natural resources such as endophytic microorganisms that colonize inner plant tissues, and which can potentially increase the tolerance to abiotic stresses. A selection of grapevine endophytes was conducted from 2021 to 2023 as part of the PRIMA project PROSIT.