In vitro tissue culture as a tool for Croatian grapevine germplasm management

Grapevine cultivars are vegetatively propagated to maintain their varietal characteristics. This process of multiplication leads to spontaneous somatic mutations that can eventually generate a variant phenotype, of potential interest for cultivar improvement and innovation. However, regardless their phenotypic effect, somatic mutations stack in the genome, and they can be used to reveal the origin and dissemination history of ancient cultivars. Here, a stringent somatic variant calling over whole genome resequencing data from 35 ‘Tempranillo Tinto’ clones or old vines from seven Iberian winemaking regions revealed 135 single nucleotide variations (SNVs) shared by some of the clonal lines.

Grapevine nitrogen (N) monitoring is essential for efficient N management plans that optimize fruit yield and quality while reducing fertilizer costs and the risk of environmental contamination. Unlike traditional vegetative-tissue sampling methods, remote sensing technologies, including hyperspectral imaging, have the potential to allow monitoring of the N status of entire vineyards at a per-vine resolution. However, differential N partitioning, variable spectral properties, and complex canopy structures hinder the development of a robust N retrieval algorithm. The present study aimed to establish a solid understanding of vine spectroscopic response at leaf and canopy levels by evaluating the different nitrogen retrieval approaches, including the radiative transfer model.

Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with plant roots and can help plants acquire nutrients from the soil in exchange for photosynthetic carbon. Commercial bioinoculants containing AMF are widely available and represent a potential opportunity to reduce the dependence of grapevines on agrochemicals. However, which commercially available AMF species colonize vine roots and affect vine growth remains unknown. The aim of this study was to identify the AMF species from commercial bioinoculants that colonize grapevine roots using high-throughput sequencing, and to evaluate the performance of five commercial bioinoculants and their effects on own-rooted Cabernet sauvignon.

Root phylloxera (Daktulosphaira vitifoliae) infestation poses a persistent threat to viticulture, yet its latent form (characterized by minimal above-ground symptoms) remains poorly understood, especially under drought stress exacerbated by climate change.

Production of volatile compounds by yeast is known to be modulated by must nitrogen. Nevertheless, various parameter of must quality have an impact on yeast fermentation. In this study we propose to evaluate the impact of nitrogen / lipids balance on a Sauvignon Blanc grape juice (Val de Loire).
Must was prepared from the same grapes at pilot scale. Three modalities were carried out: direct pressing, direct pressing with a pre-fermentation cold stabulation and pellicular maceration before pressing.