In vitro tissue culture as a tool for Croatian grapevine germplasm management

Measurements of parameters spatialy positionned, with on line sensors mounted on classical machinery or airborne imagery is no more a problem in viticulture. In a short time, high resolution data dedicated to the assessment of the vine characteristics, the soil, the harvest, etc. will become a reality.

Aging process is an essential stage in the improvement of wine quality. This process is usually performed by contact with oak wood whose compounds are released and transferred to wine, acquiring typical aging bouquet. Although the use of oak chips is a practice generally accepted as alternative to barrels to shorten aging process, the application of emerging technologies is being unfolded to accelerate this stage.

Aim: Climate is arguably one of the most important factors determining the quality of wine from any given grapevine variety. High rainfall during spring can promote growth of the vines but increases the risk of fungal disease, while vineyard operations can be disrupted, as machinery may be prevented from getting in the vineyard owing to muddy soils.

Laccases from lactic acid bacteria (LAB) are described as multicopper oxidase enzymes with copper union sites. Among their applications, phenolic compounds’ oxidation and biogenic amines’ degradation, have been described. Besides, the role of LAB in the toxicity reduction of ochratoxin A (OTA) has been reported (Fuchs et al., 2008; Luz et al., 2018). Fungal laccases, but not bacterial laccases, have been screened for OTA and mycotoxins’ degradation (Loi et al., 2018). OTA is a mycotoxin produced by some fungal species, such as Penicillium and Aspergillus sp., which infect grape bunches used for winemaking.

Wine quality depends on grape biochemical constituents, including sugars, organic acids, amino acids, and bound and free aroma compounds, which are influenced by vineyard location and environmental factors such as temperature and precipitation [1].