terclim by ICS banner
IVES 9 IVES Conference Series 9 Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Abstract

From fruit set to ripening, the grape berry mesocarp experiences a wide range of dynamic physical, physiological, and biochemical changes, such as mesocarp cell death (MCD) and hydraulic isolation. The premature occurrence of such events is a characteristic of the Niagara Rosada (NR) variety, utilised as table grapes and winemaking. In our opinion, the onset of ripening would not cause MCD, but a down-regulation of respiratory enzymes during the early loss of cell viability, while maintaining membrane integrity. For this, we investigated three distinct developmental stages (green (E-L33), veraison (E-L35), and ripe (E-L39)) of NR berries by label-free proteomics, enzymatic respiratory activity and outer mesocarp imaging. Cell wall-modifying proteins were found to accumulate differently throughout ripening, while cytoplasmic membranes continue intact. In addition, the reduction in the mitochondria cristae density occurred simultaneously with the decrease of malate dehydrogenase and succinate dehydrogenase activities. By proteomics, we identified 956 differentially accumulated proteins, of which most were down-regulated at ripening. However, several respiratory enzymes were among the most abundant proteins at ripening, showing the maintenance of respiratory activity. Furthermore, we hypothesized that gluconeogenesis originating from malate can happen in NR berries, and that sucrose futile cycles may become an important system for storing and unloading carbohydrates. Therefore, the present data indicate that the premature loss of berry mesocarp vitality in NR was not associated with cell death. Moreover, the grape variety and cultivation region can influence protein abundance, enriching our understanding of grape berry proteome and ripening dynamics in tropical conditions.

Download the abstract

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Luan Cordeiro Corrêa1, Roberta Pena de Paschoa2, Vanildo Silveira2, Ricardo Enrique Bressan-Smith1*

1 Universidade Estadual do Norte Fluminense, Plant Physiology Lab
2 Universidade Estadual do Norte Fluminense, Laboratório de Biotecnologia

Contact the author*

Keywords

grapevine, tropical viticulture, berry maturation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Regional discrimination of shiraz using targeted and non-targeted analytical approaches

Aims: Shiraz is the most widely cultivated grape variety in Australia, and is grown under a range of viticultural and climatic conditions. Given its importance to the Australian wine sector, a number of studies have been conducted in recent years which involved a comprehensive assessment of grape composition, in order to objectively predict wine quality and style outcomes.

Read More

Stabulation (lees stirring) in must as a method for aroma intensification: A comparison with skin contact and a classical version of Traminer and Sauvignon blanc in Austria

In the course of this study, stabilisation (lees stirring in unclarified must) with skin contact and classic white wine vinification were compared for the Sauvignon blanc and Traminer varieties in Austria. The test wines were analysed for the volatile substances esters, free monoterpenes and fruity thiols

Read More

Winemaking techniques and wine tasting methods at the end of the Middle Ages

Les pratiques de vinification et de dégustation du vin sont souvent perçues, à travers un discours marketing très puissant, sous l’angle d’une tradition millénaire qui perdure depuis le Moyen Âge. En Bourgogne, il est courant de rattacher les racines de ces pratiques à l’activité des institutions ecclésiastiques qui possédaient d

Read More

Description of the effect of the practical management in the characterization of « terroir effect »

The characterization of « the soil effect » in vine growing is often limited to the description of the physical components of the terroir. Many works were done in this direction and corresponded to geological, pedological or agronomical approaches. However, if the physical environment influences the vine and its grapes, its effect becomes limited at the scale of exploitation. Thus, it could be important to consider how the viticulturist « translated » the potential.

Read More

Different yield regulation strategies in semi-minimal-pruned hedge (SMPH) and impact on bunch architecture

Yields in the novel viticulture training system Semi-Minimal-Pruned Hedge (SMPH) are generally higher compared to the traditional Vertical Shoot Positioning (VSP). Excessive yields have a negative impact on the vine and wine quality, which can result in substantial losses in yield in subsequent vintages (alternate bearing) or penalties in fruit quality. Therefore yield regulation is essential. The bunch architecture in SMPH differs from VSP. Generally there is a higher amount but smaller bunches with lower single berry weights in SMPH compared to VSP.

Read More