terclim by ICS banner
IVES 9 IVES Conference Series 9 Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Abstract

From fruit set to ripening, the grape berry mesocarp experiences a wide range of dynamic physical, physiological, and biochemical changes, such as mesocarp cell death (MCD) and hydraulic isolation. The premature occurrence of such events is a characteristic of the Niagara Rosada (NR) variety, utilised as table grapes and winemaking. In our opinion, the onset of ripening would not cause MCD, but a down-regulation of respiratory enzymes during the early loss of cell viability, while maintaining membrane integrity. For this, we investigated three distinct developmental stages (green (E-L33), veraison (E-L35), and ripe (E-L39)) of NR berries by label-free proteomics, enzymatic respiratory activity and outer mesocarp imaging. Cell wall-modifying proteins were found to accumulate differently throughout ripening, while cytoplasmic membranes continue intact. In addition, the reduction in the mitochondria cristae density occurred simultaneously with the decrease of malate dehydrogenase and succinate dehydrogenase activities. By proteomics, we identified 956 differentially accumulated proteins, of which most were down-regulated at ripening. However, several respiratory enzymes were among the most abundant proteins at ripening, showing the maintenance of respiratory activity. Furthermore, we hypothesized that gluconeogenesis originating from malate can happen in NR berries, and that sucrose futile cycles may become an important system for storing and unloading carbohydrates. Therefore, the present data indicate that the premature loss of berry mesocarp vitality in NR was not associated with cell death. Moreover, the grape variety and cultivation region can influence protein abundance, enriching our understanding of grape berry proteome and ripening dynamics in tropical conditions.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Luan Cordeiro Corrêa1, Roberta Pena de Paschoa2, Vanildo Silveira2, Ricardo Enrique Bressan-Smith1*

1 Universidade Estadual do Norte Fluminense, Plant Physiology Lab
2 Universidade Estadual do Norte Fluminense, Laboratório de Biotecnologia

Contact the author*

Keywords

grapevine, tropical viticulture, berry maturation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

EXTRACTIBLE COMPOUNDS FROM MICROAGGLOMERATED CORK STOPPERS

After bottling, the wine continues to evolve during storage. The choice of the stopper is an important factor in this evolution. In addition to the oxygen permeability of the closure, the migration of stopper compounds into the wine can also have an impact on the wine organoleptic properties. Many studies have shown that transfers of volatile compounds from the stoppers into the wine can happen depending on the type of closure used (1). Moreover, when cork-made stoppers are used, the migration of phenolic compounds from the stopper into the wine can also occur (2, 3).

Aroma composition of young and aged Lugana and Verdicchio

AIM Verdicchio and Lugana are two Italian white wines produced in the Marche and Garda lake regions respectively. They are however obtained using grape varieties sharing the same genetic background, locally known as Verdicchio in Marche and Trebbiano di Soave in Garda. Anecdotal evidence suggests that these two wine types exhibit distinctive aroma features. The aim of this work was to explore the existence of a recognizable odour profile for Lugana and Verdicchio, and whether specific aroma chemical markers could be identified. METHODS 13 commercial wines, 6 Lugana and 7 Verdicchio were used. Sensory analysis was done using sorting task methodology, assessing only odor similarities. A total of 53 volatile compounds were identified and quantified GC-MS analysis. Aging behaviors were also evaluated after an accelerated aging at 40 ° C for 3 months. RESULTS HCA analysis of sorting task data identified indeed two groups: one characterized by floral and minty notes and mostly associated with Lugana wines, the other characterized by spicy and toasted aromas and mostly associated with Verdicchio. From a chemical point of view, major differences between the two wines types were observed for cis-3-hexenol, methionol, phenylethyl alcohol, and geraniol.

Impact assessment of the reverse osmosis technique in wine alcohol management

Wine authenticity and composition can be influenced by a range of membrane separation processes as reverse osmosis. In the context of climate change, the natural trend is to obtain wines with higher alcoholic concentration when classical winemaking methods are employed, and this may induce alteration of typicity of wines by masking the olfactory and taste properties. This study aimed to evaluate the influence of reverse osmosis techniques used for decrease of ethanol content on the stable isotopic ratios as markers for wine authenticity characteristics.

On quality assurance of winemaking components

This report examines product quality assurance issues arising when technological aids and food additives are utilized in winemaking.

Les activités peroxidasiques du raisin de quelques cépages de Roumanie

Les enzymes d’oxydation (polyphénoloxydase, peroxydase) des raisins sont d’origine génétique dépendantes des facteurs climatiques et agrotechniques (Sapis et al, 1983). Dans le processus technologique de l’obtention du moût de raisins, ces enzymes catalysent l’oxydation de certains composés phénoliques naturellement présents dans le raisin, produisant ainsi des modifications indésirables de la couleur et de l’arôme du vin.