terclim by ICS banner
IVES 9 IVES Conference Series 9 Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Abstract

From fruit set to ripening, the grape berry mesocarp experiences a wide range of dynamic physical, physiological, and biochemical changes, such as mesocarp cell death (MCD) and hydraulic isolation. The premature occurrence of such events is a characteristic of the Niagara Rosada (NR) variety, utilised as table grapes and winemaking. In our opinion, the onset of ripening would not cause MCD, but a down-regulation of respiratory enzymes during the early loss of cell viability, while maintaining membrane integrity. For this, we investigated three distinct developmental stages (green (E-L33), veraison (E-L35), and ripe (E-L39)) of NR berries by label-free proteomics, enzymatic respiratory activity and outer mesocarp imaging. Cell wall-modifying proteins were found to accumulate differently throughout ripening, while cytoplasmic membranes continue intact. In addition, the reduction in the mitochondria cristae density occurred simultaneously with the decrease of malate dehydrogenase and succinate dehydrogenase activities. By proteomics, we identified 956 differentially accumulated proteins, of which most were down-regulated at ripening. However, several respiratory enzymes were among the most abundant proteins at ripening, showing the maintenance of respiratory activity. Furthermore, we hypothesized that gluconeogenesis originating from malate can happen in NR berries, and that sucrose futile cycles may become an important system for storing and unloading carbohydrates. Therefore, the present data indicate that the premature loss of berry mesocarp vitality in NR was not associated with cell death. Moreover, the grape variety and cultivation region can influence protein abundance, enriching our understanding of grape berry proteome and ripening dynamics in tropical conditions.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Luan Cordeiro Corrêa1, Roberta Pena de Paschoa2, Vanildo Silveira2, Ricardo Enrique Bressan-Smith1*

1 Universidade Estadual do Norte Fluminense, Plant Physiology Lab
2 Universidade Estadual do Norte Fluminense, Laboratório de Biotecnologia

Contact the author*

Keywords

grapevine, tropical viticulture, berry maturation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Viticulture and climate: from global to local

Aims: This review aims to (1) present the multiple interests of studying and depicting and climate spatial variability for vitivinicultural terroirs study; (2) explain the factors that affect climate spatial variability according to the spatial scale considered and (3) provide guidelines for climate zoning considering challenges linked to each methodology considered.

Yeast derivatives: a promising alternative in wine oxidation prevention?

Oxidation processes constitute a main problem in winemaking. Oxidation result in color browning and varietal aroma loss, which are key attributes of wine organoleptic quality

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Conduite en Lys: résultats pendant la formation du système avec le cépage Loureiro dans la région des “Vinhos Verdes”

Dans la région des “Vinhos Verdes” les études sur les systèmes de conduite de la vigne sont très importantes et beaucoup de travaux ont été faits pendant les dernières années. Cet essai

Carbon isotope discrimination in berry juice sugars: changes in response to soil water deficits across a range of vitis vinifera cultivars

In wine producing regions around the world, climate change has the potential to decrease the frequency and amount of precipitation and increase average and extreme temperatures. This will lower soil water availability and increase evaporative demand, thereby increasing the frequency and intensity of water deficit experienced in vineyards. Among other things, grapevines manage water deficit by regulating stomatal closure. The dynamics of this regulation, however, have not been well characterized across the range of Vitis vinifera cultivars. Providing a method to understand how different cultivars regulate their stomata, and hence water use in response to changes in soil water deficits will help growers manage vineyards and select plant material to better meet quality and yield objectives in a changing climate.