terclim by ICS banner
IVES 9 IVES Conference Series 9 Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Proteomic profiling of grape berry presenting early loss of mesocarp cell vitality

Abstract

From fruit set to ripening, the grape berry mesocarp experiences a wide range of dynamic physical, physiological, and biochemical changes, such as mesocarp cell death (MCD) and hydraulic isolation. The premature occurrence of such events is a characteristic of the Niagara Rosada (NR) variety, utilised as table grapes and winemaking. In our opinion, the onset of ripening would not cause MCD, but a down-regulation of respiratory enzymes during the early loss of cell viability, while maintaining membrane integrity. For this, we investigated three distinct developmental stages (green (E-L33), veraison (E-L35), and ripe (E-L39)) of NR berries by label-free proteomics, enzymatic respiratory activity and outer mesocarp imaging. Cell wall-modifying proteins were found to accumulate differently throughout ripening, while cytoplasmic membranes continue intact. In addition, the reduction in the mitochondria cristae density occurred simultaneously with the decrease of malate dehydrogenase and succinate dehydrogenase activities. By proteomics, we identified 956 differentially accumulated proteins, of which most were down-regulated at ripening. However, several respiratory enzymes were among the most abundant proteins at ripening, showing the maintenance of respiratory activity. Furthermore, we hypothesized that gluconeogenesis originating from malate can happen in NR berries, and that sucrose futile cycles may become an important system for storing and unloading carbohydrates. Therefore, the present data indicate that the premature loss of berry mesocarp vitality in NR was not associated with cell death. Moreover, the grape variety and cultivation region can influence protein abundance, enriching our understanding of grape berry proteome and ripening dynamics in tropical conditions.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Luan Cordeiro Corrêa1, Roberta Pena de Paschoa2, Vanildo Silveira2, Ricardo Enrique Bressan-Smith1*

1 Universidade Estadual do Norte Fluminense, Plant Physiology Lab
2 Universidade Estadual do Norte Fluminense, Laboratório de Biotecnologia

Contact the author*

Keywords

grapevine, tropical viticulture, berry maturation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Nitrogen uptake, translocation and YAN in berries upon water deficit in grapevines with contrasting stomatal sensitivity

Nitrogen (N2) is critical in grape berries, especially in organic wine making. After intake, N2 follows various metabolic and allocation routes and, from veraison, partly reallocates into berries. Water deficit affects the N2 nutrition due to a poor diffusion in soil solution and vascular mobilisation. Also, affects photosynthesis and the energy needed for metabolism, whose extent would depend on the stomatal sensitivity of the plant. We have assessed the effect of a moderate water deficit from pea size, in 3 years old field grown potted plants of Chardonnay (CH) and Cabernet Sauvignon (CS), differing in stomatal sensitivity, on the N2 status of plant parts. Water deficit reduced photosynthesis, leaf area and fresh and dry plant mass along the season, but up to a higher extent in CS.

Untargeted LC-HRMS analysis to discover new taste-active compounds in spirits.

​For several years, the chemistry of taste has aroused high interest both from academics and industrials. Plant kingdom is a rich and reliable source of new taste-active compounds. Many sweet, bitter or sour molecules have been identified in various plants [1]. They belong to diverse chemical families and their sensory properties are strongly affected by slight structural modifications. As a consequence, the investigation of natural taste-active products in a given matrix appears as a major challenge for chemists. Such studies are particularly relevant in oenology since they allow a better understanding of wine and spirit taste.

The Cognac industry: history, successes and challenges

With alcohol consumption steadily declining, the growing popularity of dry january, a fiercely competitive environment, high dry matter inflation, economic upheavals, commercial uncertainties… The wine industry must adapt and offer products that meet consumer expectations, without denying their historical singularities.

Influence on grape aroma of nitrogen compounds and elicitors foliar applications in vineyards

The grape volatile compounds determine the wine quality and typicity [1]. Thus, looking for agronomic tools to improve its composition it is of great interest in the sector [2]

Bud fruitfulness in Vitis vinifera L. cv. Chardonnay in cool climate regions in South Africa

Bud fruitfulness is a key determinant of the potential and the actual yield. The formation of the grapevine yield spans over a period of two consecutive growing seasons (Ferrara & Mazzeo, 2023).