terclim by ICS banner
IVES 9 IVES Conference Series 9 Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

Abstract

Grapevine cultivars are vegetatively propagated to maintain their varietal characteristics. This process of multiplication leads to spontaneous somatic mutations that can eventually generate a variant phenotype, of potential interest for cultivar improvement and innovation. However, regardless their phenotypic effect, somatic mutations stack in the genome, and they can be used to reveal the origin and dissemination history of ancient cultivars. Here, a stringent somatic variant calling over whole genome resequencing data from 35 ‘Tempranillo Tinto’ clones or old vines from seven Iberian winemaking regions revealed 135 single nucleotide variations (SNVs) shared by some of the clonal lines. Among them, we selected 56 highly informative SNVs to custom-design a high-throughput genotyping chip for this cultivar, which was validated and further tested in 94 ‘Tempranillo Tinto’ vines from highly diverse regions. Phylogenetic analyses revealed that ‘Tempranillo Tinto’ samples grouped in three major clonal lineages, a clustering that reflected the geographic origin of the samples. After combining these results with genome re-sequencing data from the two ‘Tempranillo Tinto’ parents, we found the Ebro River Valley as the most likely region of origin for ‘Tempranillo Tinto’. Results also revealed one major historical dissemination route that likely progressed westwards from this original site, towards the main winemaking regions found across the Duero River Valley and to the South in Portugal. Collectively, the results obtained in this study shed light on the origin and historical dispersal of ‘Tempranillo Tinto’ in the Iberian Peninsula, and released highly informative SNVs for the differentiation of intra-cultivar lineages.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Javier Tello1*, Pablo Carbonell-Bejerano1, Rafael Torres-Pérez2, Yolanda Ferradás1,3, Carolina Royo1, Juan Carlos Oliveros2, Javier Ibáñez1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño.
2 Centro Nacional de Biotecnología, C/Darwin 3, 28049 Madrid.
3 Current address: Facultad de Biología, Universidad de Santiago de Compostela, 15872 Santiago de Compostela

Contact the author*

Keywords

clonal lineage, grapevine, Iberian Peninsula, phylogenomics, SNV

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Soil chemistry as a measure of the distinctiveness of american viticultural areas of the Columbia basin, USA

The Columbia Basin, a semi-arid region centered in the eastern part of Washington State, is the second largest wine grape growing region in the United States and presently contains 10 American Viticultural Areas

Nucleophilic fraction to estimate the antioxidant activity of inactivated yeast derivates

Oxidation in wine is mostly related to the Michael addition of nucleophiles on two quinones formed from the oxidation of ortho-diphenols. In wine this mechanism is responsible for the increase of the yellow hue and aroma loss. Glutathione exerts its antioxidant activity throughout its competitive addition onto quinones, but many other compounds can have the same behavior: sulfanyl
compounds, amino acids, etc. Addition of yeast derivates during the winemaking process can increase the level of those nucleophilic compounds and then confer to the wine a higher resistance

Zoning mountain landscapes for a valorisation of high identity products

Mountain agriculture is made difficult by the geomorphological complexity of the territory. This is especially true for viticulture: over the centuries the work of men in such a difficult environment

Viticultural characterisation of soils from triassic period at Beaumes-de-Venise (Côtes du Rhône, France)

Wineries of Beaumes-de-Venise area make their best red wines with grapes from the “Triassic terroir”. This « terroir » is characterized by soils from the Triassic period. These specific soils are complex and quite heterogeneous. They originate from an eventful geological history to keep in mind to understand soils geographical distribution.

An effective approach to mitigating ochratoxin A (OTA) levels in wine with minor impact on wine quality

OTA occurrence in wine is well-documented, with higher levels typically found in red (< 0.01-7.63 μg/l), followed by rose (0.01-2.40 μg/l) and white wine (<0.01-1.72 μg/l). Incidence rates are nOTAble, with studies showing OTA present in 53% of 521 red wines, 69% of 98 rose, and 61% of 301 white wines analysed. In europe, wine is estimated to be the second source of OTA intake after cereals. Since 2006, the maximum allowable limit for OTA in wine is 2 μg/l, according to regulation (ec) no. 1881/2006.