terclim by ICS banner
IVES 9 IVES Conference Series 9 Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

Abstract

Grapevine cultivars are vegetatively propagated to maintain their varietal characteristics. This process of multiplication leads to spontaneous somatic mutations that can eventually generate a variant phenotype, of potential interest for cultivar improvement and innovation. However, regardless their phenotypic effect, somatic mutations stack in the genome, and they can be used to reveal the origin and dissemination history of ancient cultivars. Here, a stringent somatic variant calling over whole genome resequencing data from 35 ‘Tempranillo Tinto’ clones or old vines from seven Iberian winemaking regions revealed 135 single nucleotide variations (SNVs) shared by some of the clonal lines. Among them, we selected 56 highly informative SNVs to custom-design a high-throughput genotyping chip for this cultivar, which was validated and further tested in 94 ‘Tempranillo Tinto’ vines from highly diverse regions. Phylogenetic analyses revealed that ‘Tempranillo Tinto’ samples grouped in three major clonal lineages, a clustering that reflected the geographic origin of the samples. After combining these results with genome re-sequencing data from the two ‘Tempranillo Tinto’ parents, we found the Ebro River Valley as the most likely region of origin for ‘Tempranillo Tinto’. Results also revealed one major historical dissemination route that likely progressed westwards from this original site, towards the main winemaking regions found across the Duero River Valley and to the South in Portugal. Collectively, the results obtained in this study shed light on the origin and historical dispersal of ‘Tempranillo Tinto’ in the Iberian Peninsula, and released highly informative SNVs for the differentiation of intra-cultivar lineages.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Javier Tello1*, Pablo Carbonell-Bejerano1, Rafael Torres-Pérez2, Yolanda Ferradás1,3, Carolina Royo1, Juan Carlos Oliveros2, Javier Ibáñez1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño.
2 Centro Nacional de Biotecnología, C/Darwin 3, 28049 Madrid.
3 Current address: Facultad de Biología, Universidad de Santiago de Compostela, 15872 Santiago de Compostela

Contact the author*

Keywords

clonal lineage, grapevine, Iberian Peninsula, phylogenomics, SNV

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Riesling aroma composition in light of changing global temperatures – delving into the effects of warmer nights on the volatile profile of riesling grapes

Climate is a key parameter when the modulation of berry and subsequent wine composition is considered. Recent decades have already seen an increase in global surface temperatures

Application of GiESCO “bio-metaethics” charter in practice: the “direct” involvement of vine grower, winemaker, society

On the basis of a direct agreement between the GiESCO and the vine grower, the winemaker and the consumers (individual; company; public or private organizations), the communication on the content of the charter can be done as follows:
• Commitment to respect the basic rules of the GiESCO “BIO – MetaEthics” charter.
1/ Put Mankind in the depth of all concerns in a universal context: (grower, consumer, citizen, work valuing, education, security)
2/ Insure minimum impact on environment by optimizing cultivation technics: (maximum of natural biodegradable products, friendly practices, short channels, renewable energies, terroir sustainability)

Sensory and nephelometric analysis of tannin fractions obtained by ultrafiltration of red wines

The assessment of red wine mouthfeel relies primarily on the sensory description of its tannic properties. This evaluation could be improved by gaining a better understanding of the physicochemical properties of these tannins. Hence, the objectives of the present study were threefold: (1) to gain an insight into the sensory properties of subpopulations of proanthocyanidic tannins of different molecular sizes obtained through several ultrafiltration steps, (2) to quantify the kinetics of haze formation of these proanthocyanidic tannins in a dynamic polyvinylpyrrolidone (PVP) precipitation test, (3) to determine whether a correlation exists between the sensory and the precipitation data.

Bunch placement effects on dehydration kinetics and physico-chemical composition of Nebbiolo grapes

Sforzato di Valtellina DOCG is a special reinforced red wine produced using withered Nebbiolo grapes. The withering process takes place in traditional rooms under natural environmental conditions; it starts immediately after the harvest and ends not before the 1st December of the same year. The process can be performed with different bunch placements that can influence the grapes features.The purpose of the study is to compare the effect on grape physico-chemical parameters for four withering bunch placement systems: hanged clusters (HC), plastic crates (CT), breathable mesh fabric on wooden frames panels (MF), and reed mats (RM). For all the systems studied, the withering length was two months at a temperature between 6 and 19 °C and a relative humidity of 41-88%.

Il paesaggio delle alberate aversane ed il vino Asprinio

Nel corso del 2009, in alcuni vigneti allevati ad alberata in provincia di Caserta (Italia), è stata avviata una ricerca per valutare la variabilità genetica della popolazione del vitigno ‘Asprinio’, la condizione sanitaria delle piante e le caratteristiche del vino sia rispetto alla forma di allevamento (alberata tradizionale e controspalliera) che all’altezza della fascia produttiva.