terclim by ICS banner
IVES 9 IVES Conference Series 9 Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

Abstract

Grapevine cultivars are vegetatively propagated to maintain their varietal characteristics. This process of multiplication leads to spontaneous somatic mutations that can eventually generate a variant phenotype, of potential interest for cultivar improvement and innovation. However, regardless their phenotypic effect, somatic mutations stack in the genome, and they can be used to reveal the origin and dissemination history of ancient cultivars. Here, a stringent somatic variant calling over whole genome resequencing data from 35 ‘Tempranillo Tinto’ clones or old vines from seven Iberian winemaking regions revealed 135 single nucleotide variations (SNVs) shared by some of the clonal lines. Among them, we selected 56 highly informative SNVs to custom-design a high-throughput genotyping chip for this cultivar, which was validated and further tested in 94 ‘Tempranillo Tinto’ vines from highly diverse regions. Phylogenetic analyses revealed that ‘Tempranillo Tinto’ samples grouped in three major clonal lineages, a clustering that reflected the geographic origin of the samples. After combining these results with genome re-sequencing data from the two ‘Tempranillo Tinto’ parents, we found the Ebro River Valley as the most likely region of origin for ‘Tempranillo Tinto’. Results also revealed one major historical dissemination route that likely progressed westwards from this original site, towards the main winemaking regions found across the Duero River Valley and to the South in Portugal. Collectively, the results obtained in this study shed light on the origin and historical dispersal of ‘Tempranillo Tinto’ in the Iberian Peninsula, and released highly informative SNVs for the differentiation of intra-cultivar lineages.

Download the abstract

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Javier Tello1*, Pablo Carbonell-Bejerano1, Rafael Torres-Pérez2, Yolanda Ferradás1,3, Carolina Royo1, Juan Carlos Oliveros2, Javier Ibáñez1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño.
2 Centro Nacional de Biotecnología, C/Darwin 3, 28049 Madrid.
3 Current address: Facultad de Biología, Universidad de Santiago de Compostela, 15872 Santiago de Compostela

Contact the author*

Keywords

clonal lineage, grapevine, Iberian Peninsula, phylogenomics, SNV

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

For a phenomenology of terroir. A consumers’ perspective

This study investigates the notion of terroir by applying a phenomenological approach, focusing on the subjective experience of consumers. We will consider how terroir is described by consumers in order to gauge their subjective viewpoint and understand their way of describing and defining this spatiality.

Read More

Influence of grape withering on corvina and corvinone aroma composition

AIM:Valpolicella is a wine region located in Italy north-east, famous for the production of dry and sweet red wines from withered grapes, including Amarone and Recioto. The aim of this study is to understand the influence of the withering process on Corvina and Corvinone wines aroma profiles. METHODS:Wines were produced with a standard red wine winemaking protocol with Corvina and Corvinone grapes from different Valpolicella vineyards and vintages. In consideration of the local traditional practice of post-harvest withering of the grapes, wines from each vineyard were obtained from either fresh and withered grapes. Wines were analysed by Solid Phase Extraction and Solid Phase Micro Extraction gas chromatography coupled to mass spectrometry.

Read More

Highlighting a link between the structure of mannoproteins and their foaming properties in sparkling wines

Effervescence and foaming properties are the main visual characteristics assessed by the consumer during
sparkling wine tasting.

Read More

Discrimination of monovarietal Italian red wines using derivative voltammetry

Identification of specific analytical fingerprints associated to grape variety, origin, or vintage is of great interest for wine producers, regulatory agencies, and consumers. However, assessing such varietal fingerprint is complex, time consuming, and requires expensive analytical techniques. Voltammetry is a fast, cheap, and user-friendly analytical tool that has been used to investigate and measure wine phenolics.

Read More

The heritage behind the very old vineyards – The novelty with tradition for the future 

In Portugal, the prospection and conservation of representative samples of intra-varietal variability of grapevine has been carried out for 46 years, and in 2010 an infrastructure was created for the conservation of all these genetic resources – the portuguese association for grapevine diversity (porvid) experimental centre for the conservation of grapevine diversity. the aim is to save the genetic identity of ancient varieties to prevent their imminent loss and to preserve the raw material for current and future selections, thus adding economic value and sustainability to the vine and wine sector.

Read More