terclim by ICS banner
IVES 9 IVES Conference Series 9 Digitalization and valorization of the genotypic and phenotypic information retained within the FEM grapevine germplasm

Digitalization and valorization of the genotypic and phenotypic information retained within the FEM grapevine germplasm

Abstract

The maintenance and valorization of genetic diversity is an undoubtable resource for the viticulture of the future, since the climate crisis is forcing us to think of new, more resilient varieties. For this reason, the grapevine germplasm of the Fondazione Edmund Mach has been continuously expanded in the last decade to a total of 3,120 accessions, whose trueness-to-type has been verified by means of the universal set of nine microsatellites. About two thirds are V. vinifera subsp. vinifera accessions, while the rest consists of naturalized and selected hybrids, V. vinifera subsp. sylvestris, and pure species. The genetic material has also been characterized over three consecutive years for ampelographic, vine development, and biotic stress response traits to be exploited for experimental purposes. All the data and metadata have been digitalized and hosted in a SQL database, the FEMVitisDB, developed with an ontology driven paradigm to annotate the deposited information. The database was built following the MIAPPE checklist to ensure data FAIRness. A RESTful WebServiceAPI based on BrAPI and a web frontend were developed to easily explore the information in the repository.
Findings about the captured genetic diversity, the identified unique profiles, and the scouted unknown and therefore novel genotypes will be discussed. The latter enrich the genetic asset of the grapevine community, towards the feeding of international databases. Where feasible, the first degree of parentage relationship has been reconstructed. Finally, the outcomes regarding the inferred phenological core collections will be introduced to provide an information arsenal for future ’omics analyses.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Paola Bettinelli1*, Daniela Nicolini1, Giulia Betta1, Daniele Migliaro2, Laura Costantini1, Geovani Luciano de Oliveira3, Silvano Clementi1, Luca Zulini1, Paolo Fontana1, Luca Bianco1, Marco Stefanini1, Diego Micheletti, Silvia Vezzulli

1 Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Trento), Italy
2 CREA – Research Centre for Viticulture and Enology, Conegliano (Treviso), Italy
3 Molecular Biology and Genetic Engineering Center (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil

§ equally contributed

Contact the author*

Keywords

Database, collection, breeding, kinship, Vitis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

DETERMINATION OF MINERAL COMPOSITION IN CV. TERAN (VITIS VINIFERA L.) RED WINE AFFECTED BY PRE-FERMENTATIVE MASH COOLING, HEATING, SAIGNÉE TECHNIQUE AND PROLONGED POST-FERMENTATIVE MACERATIONS

This study aimed to determine mineral composition in red wine obtained from cv. Teran (Vitis vinifera L.), autochtonous Croatian grape variety. Six different vinification treatments, including the control treatment (7-day standard maceration), were performed to study the effects of: 48-hour pre-fermentative mash cooling (8 °C) followed by prolonged post-fermentative maceration of 13 days (C15), 28 days (C30), and saignée technique (juice runoff) proceeded with prolonged post-fermentative maceration of 13 days (CS15); and effect of 48-hour heating (50 °C) followed by prolonged post-fermentative maceration of 13 days (H15) and 28 days (H30) on macro- and microelements in wine.

Elucidating contributions by vineyard site on volatile aroma characteristics of pinot noir wines

Correlations between vineyard site and wine have, historically, been limited due to lack of uniformity in scion and rootstock clone and lack of controlled pilot-scale winemaking conditions, particularly temperature

Genetic traceability of the varietal origin of wines: a robust application for must and wines during alcoholic fermentation

Industry and regulatory agencies have developed regulations to ensure authenticity and compliance with wine composition limits. However, this can be truncated by the absence of simple and robust analytical methodologies, uninfluenced by the environment, different oenological techniques and cultural practices. Genetic fingerprinting is the most powerful tool for unequivocal varietal identification; it is not affected by the environment or agronomic practices; however, its usefulness in musts and wines has been controversial and there is currently no routine certification of varietal origin based on DNA analysis.

Exploiting somaclonal variability to increase drought stress tolerance in grapevine 

Global warming has enhanced the frequency and severity of drought events, hence calling for a better management of water resources in the vineyard and for an improvement of breeding platforms. Somatic embryogenesis (SE) (i.e. the initiation of embryos from somatic tissues) can spontaneously generate new genetic variability, which results from genetic mutations, changes in epigenetic marks, or phenotypic alterations.
This study was tailored to test whether vines in vitro regenerated through SE (i.e. somaclones), can tolerate water deprivation better than the mother plant.

Relationship between chemical parameters of tannins and in-mouth attributes of grape phenolic fractions

Establish relationships between taste and mouthfeel properties of grapes and tannin-related chemical parameters. Tempranillo Tinto and Garnacha Tinta grapes were harvested from distinct blocks in different dates; each sample collection date was separated by one week. Grapes were destemmed and macerated in 15% of ethanol for one week. The polyphenolic fraction (PF) of samples was submitted to solid phase extraction on C18 cartridges and recovered with ethanol. PFs were reconstituted in wine model and their taste and mouthfeel properties were characterised by rate-K-attributes methodology. In parallel, concentration (TC) and activity (TAc) of tannins as well as the concentration of tannins linked to anthocyanins (T-A) were determined using HPLC-UV–VIS.