Investigating water stress-related seasonal and spatial patterns and the possible links with juice and wine compositional parameters

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

The adaptation of rootstock to scion variety and soil determines largely the control of the vegetative growth for grapevine. Many experiments were performed in the vineyard to classify the rootstocks according to their soil adaptation and to their effect on vine vigour. So far there are no data describing the course of appearance of rootstock effects after plantation. Moreover the underlying mechanisms of conferred vigour remain largely unknown.

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.

Les indices bioclimatiques constituent un bon outil pour piloter le développement vitivinicole dans une région précise

Context and purpose of the study. Globally, vineyard cultivation uses conventional methods to manage pests, diseases and increase yield.