Investigating water stress-related seasonal and spatial patterns and the possible links with juice and wine compositional parameters

On the basis of a direct agreement between the GiESCO and the vine grower, the winemaker and the consumers (individual; company; public or private organizations), the communication on the content of the charter can be done as follows:
• Commitment to respect the basic rules of the GiESCO “BIO – MetaEthics” charter.
1/ Put Mankind in the depth of all concerns in a universal context: (grower, consumer, citizen, work valuing, education, security)
2/ Insure minimum impact on environment by optimizing cultivation technics: (maximum of natural biodegradable products, friendly practices, short channels, renewable energies, terroir sustainability)

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes
such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species
diversity of many of the relevant ecological niches within the broader wine environment, from vineyard
soils to plants and grapes to fermentation. The data highlight that species identities and diversity
significantly impact agronomic performance of vineyards as well as wine quality, but the complexity
of these systems and of microbial growth dynamics has defeated attempts to offer actionable
tools to guide or predict specific outcomes of ecosystem-based interventions.

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.

‘Tempranillo Tinto’ is one of the most relevant grapevine cultivars worldwide. Despite its early ripening and relatively short vegetative cycle, which may not be ideal for high-quality grape and wine production in warming conditions, its long-standing cultivation has led to an intense multiplication by cuttings, which originated the high level of clonal variation currently available. Now, this intra-varietal diversity provides an interesting opportunity for cultivar improvement by identifying genotypes with better adaptation potential.

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors. The impact on wine aroma is mainly attributed to volatile phenols, while in-mouth hydrolysis of glycosylated forms may be responsible for long-lasting “ashy” aftertastes (1).