terclim by ICS banner
IVES 9 IVES Conference Series 9 Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes

Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes

Abstract

Rachis browning is a common abiotic stress that occurs during postharvest storage, leading to a decrease in commercial value of table grapes and resulting in significant economic losses. Its early detection could enable the implementation of preventive strategies. In this report, we show the feasibility of a non-destructive early detection of browning based on Hyper Spectral Imaging (HSI). Furthermore, rachis samples were subjected to transcriptomic analysis to understand putative pathways causing differences in browning within varieties. The onset of visible browning of rachis was quantified with ImageJ software analysis in six different table grape varieties (red and white). The varieties investigated are novel table grapes obtained in an ongoing breeding program at CREA-VE in Southern Italy. After harvesting, the bunches were packed in cardboard boxes and stored for two weeks at 2°C with 95% relative humidity to evaluate the shelf-life. The berries were not removed from the grape bunch to follow the onset of browning on the same bunches. Anyway, due to differences in cluster and berry size, the internal area of the rachides was not always visible. Therefore, changes were followed in the apical portion of the rachides. A Specim IQ camera was employed for image detection in the laboratory and the Specim IQ Studio software was used to detect Areas Of Interest (AOIs). Differences in both the visible and Near Infra-Red (NIR) spectral regions were detected before the onset of browning of the rachides. It was possible to observe different changes in the spectral profiles among the varieties.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Teodora Basile1*, Carlo Bergamini1, Lucia Rosaria Forleo1, Flavia Angela Maria Maggiolini1, Margherita D’Amico1, Antonio Domenico Marsico1, Rocco Perniola1, Luca Nerva2, Walter Chitarra2, Maria Francesca Cardone1

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), via Casamassima 148, 70010 Turi (BA), Italy
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (TV), Italy

Contact the author*

Keywords

Vitis vinifera, postharvest, image analysis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Bioanalytical workflow for exploring the chemical diversity and antioxidant capacity of grape juice peptides

The oxidative stability of white wines is related to a flow of chemical reactions involving a number of native wine containing compounds composing their antioxidant metabolome.

Mechanization of pre-flowering leaf removal under the temperate-climate conditions of Switzerland

Grapevine leaf removal (LR) in the cluster area is typically done between fruit set and cluster closure to create an unfavorable microclimate for fungal diseases, such as Botrytis cinerea and powdery mildew. Grape growers are now turning their attention to pre-flowering LR, which has additional benefits under certain conditions. When applied before flowering, LR strongly affects fruit set and thus the number of berries per cluster. It is therefore a good yield control tool, replacing time-consuming manual cluster thinning (Poni et al. 2006). It also improves berry structure, that is, skin thickness, skin-to-pulp ratio, and berry composition (total soluble solids, titratable acidity, and polyphenols) (Palliotti et al. 2012; Komm and Moyer 2015). By exacerbating competition for assimilates between reproductive and vegetative organs, pre-flowering LR also poses some risks. Excessive yield loss at the same year’s harvest due to a too low fruit set rate is the main concern: intensive pre-flowering LR (100% of the cluster area) can induce up to 50% yield loss in potted vines (Poni et al. 2005). Other parameters, such as cool climatic conditions during flowering, also affect fruit set rate and make it difficult to predict potential yield at harvest. Repeated and overly intensive preflowering LR can have repercussions over time and induce a decline in bud fruiting and plant vigor (Risco et al. 2014).

Redwine project: increasing microalgae biomass feedstock by valorising wine gaseous and liquid residues

Global warming due to greenhouse gases (GHG) has become a serious worldwide concern.

Swiss program for the creation of fungal disease resistant grape varieties in Switzerland

Grapevine breeding is part of the research program of Agroscope in Switzerland since 1965. From 1965 to 1995, the aim of the Vitis vinifera crosses was to obtain a high resistance to grey rot (Botrytis cinerea), one of the most virulent fungal pathogens in the Swiss vineyard. In 2021, the grape varieties released from this first breeding program covered 936 ha of the 15’000 ha of the Swiss vineyard.
In 1996, a second breeding program aimed at obtaining, by classical interspecific hybridization, grape varieties resistant to downy mildew (Plasmopara viticola) and powdery mildew (Erisyphe necator) and less sensitive to grey rot (Botrytis cinerea). In order to accelerate and make the selection process more reliable, an early biochemical test was developed based on the natural defense mechanisms of the vine against downy mildew (stilbene phytoalexins). The synthesis of stilbenes (i.e., resveratrol and its oxidized dimers - and -viniférine) and pterostilbenes (methylated derivative) is among the most efficient induced defense mechanisms of grapevine against fungal pathogens on both the leaves and the clusters.

Effect of different winemaking techniques and grape variety on chemo-sensory parameters of white wines

AIM: Study the chemical and sensory parameters of fifty commercial white wines elaborated with different techniques (fermented in oak barrel and aged on lees (FB+AL); aged on lees (AL); and without aging (WA)) and different grape varieties (Verdejo, Sauvignon blanc and Godello).