terclim by ICS banner
IVES 9 IVES Conference Series 9 Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes

Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes

Abstract

Rachis browning is a common abiotic stress that occurs during postharvest storage, leading to a decrease in commercial value of table grapes and resulting in significant economic losses. Its early detection could enable the implementation of preventive strategies. In this report, we show the feasibility of a non-destructive early detection of browning based on Hyper Spectral Imaging (HSI). Furthermore, rachis samples were subjected to transcriptomic analysis to understand putative pathways causing differences in browning within varieties. The onset of visible browning of rachis was quantified with ImageJ software analysis in six different table grape varieties (red and white). The varieties investigated are novel table grapes obtained in an ongoing breeding program at CREA-VE in Southern Italy. After harvesting, the bunches were packed in cardboard boxes and stored for two weeks at 2°C with 95% relative humidity to evaluate the shelf-life. The berries were not removed from the grape bunch to follow the onset of browning on the same bunches. Anyway, due to differences in cluster and berry size, the internal area of the rachides was not always visible. Therefore, changes were followed in the apical portion of the rachides. A Specim IQ camera was employed for image detection in the laboratory and the Specim IQ Studio software was used to detect Areas Of Interest (AOIs). Differences in both the visible and Near Infra-Red (NIR) spectral regions were detected before the onset of browning of the rachides. It was possible to observe different changes in the spectral profiles among the varieties.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Teodora Basile1*, Carlo Bergamini1, Lucia Rosaria Forleo1, Flavia Angela Maria Maggiolini1, Margherita D’Amico1, Antonio Domenico Marsico1, Rocco Perniola1, Luca Nerva2, Walter Chitarra2, Maria Francesca Cardone1

1 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), via Casamassima 148, 70010 Turi (BA), Italy
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (TV), Italy

Contact the author*

Keywords

Vitis vinifera, postharvest, image analysis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Use of ultrasounds to accelerate aging on lees of red wines

Aging on lees (AOL) is a powerful technique to protect varietal aroma and color. Simultaneously, helps to soften tannins and increase and improve wine body and structure. AOL is complementary to barrel aging modulating the wood impact and protecting wine from oxidative conditions.

The socioclimatic dynamics and the table grape production during a long-drought: the case of Brazilian semiarid

In 2022, the area cultivated with grapes in Brazil counted 75 thousand ha. About 1/2 of the grape production is located in rio grande do sul state, in South Brazil. Nonetheless, the northeast region, especially the Sao Francisco River Valley (SFRV), is increasing its area and production, mainly pushed by table grapes. The states of bahia and pernambuco already respond for circa 1/3 of brazilian grape production.

USE OF 13C CP/MAS NMR AND EPR SPECTROSCOPIC TECHNIQUES TO CHARACTERIZE MACROMOLECULAR CHANGES IN OAK WOOD(QUERCUS PETRAEA) DURING TOASTING

For coopers, toasting process is considered a crucial step in barrel production during which oak wood (Q. petraea) develops several aromatic nuances released to the wine during its maturation. Toasting consists of applying different degrees of heat to a barrel for a specific period. As the temperature increases, thermal degradation of oak wood structure produces a huge range of chemical compounds. Many studies have identified the main key aroma volatile compounds (whisky-lactone, furfural, eugenol, guaiacol, vanillin). However, detailed information on how the chemical structure of oak wood degrades with increasing toasting level is still lacking.

Understanding graft union formation by using metabolomic and transcriptomic approaches during the first days after grafting in grapevine

Since the arrival of Phyloxera (Daktulosphaira vitifolia) in Europe at the end of the 19th century, grafting has become essential to cultivate Vitis vinifera. Today, grafting provides not only resistance to this aphid, but it used to adapt the cultivars according to the type of soil, environment, or grape production requirements by using a panel of rootstocks. As part of vineyard decline, it is often mentioned the importance of producing quality grafted grapevine to improve vineyard longevity, but, to our knowledge, no study has been able to demonstrate that grafting has a role in this context. However, some scion/rootstock combinations are considered as incompatible due to poor graft union formation and subsequently high plant mortality soon after grafting. In a context of climate change where the creation of new cultivars and rootstocks is at the centre of research, the ability of new cultivars to be grafted is therefore essential. The early identification of graft incompatibility could allow the selection of non-viable plants before planting and would have a beneficial impact on research and development in the nursery sector. For this reason, our studies have focused on the identification of metabolic and transcriptomic markers of poor grafting success during the first days/week after grafting; we have identified some correlations between some specialized metabolites, especially stilbenes, and grafting success, as well as an accumulation of some amino acids in the incompatible combination. The study of the metabolome and the transcriptome allowed us to understand and characterise the processes involved during graft union formation.

Long-lasting flavour perception of wines treated with oenological additives considering the individual PROP taste-phenotype

The use of oenological additives is becoming a common practice due to the technological and sensory properties they provide to the wines. However, the number of studies focused on the impact that these additives might induce on wine flavor perception during wine tasting is still quite scarce. The aim of this work was to evaluate the effect of three different types of common oenological additives: two oenotannins (ellagitannin and gallotannin) and a commercial preparation of yeast mannoproteins on the long-lasting flavor perception (aroma and astringency).