Application of Hyper Spectral Imaging for early detection of rachis browning in table grapes

Carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) are the greenhouse gases (GHG) that have suffered a more marked concentration increase since the Industrial Revolution, with agriculture accounting for 13.5% of the worldwide emissions.

Rapid processing of grapes after harvest has always been considered essential for achieving a balanced sensory wine profile.

The market trend heads to food products with less chemical inputs, including in oenology. During the winemaking process, sulfites are commonly use to avoid microbiological contamination and stabilization of the wine thanks to its antimicrobial and antioxidant activities. Nevertheless, this use is not without consequences on human health and environment, leading for example to allergic reaction and pollution. A biological alternative to these sulfites has emerges: the bioprotection.

Black rot, caused by Phyllosticta ampelicida (Guignardia bidwellii), is a serious fungal disease in viticulture. Incidences increased in recent years due to reduced pesticide treatments, neglected phytosanitary measurements, and effects of climate change.

The recent development of regulatory genomics has raised increasing interest in plant research since transcriptional regulation of genes plays a pivotal role in many biological processes. By shedding light on the target genes of the various transcription factors (TFs), it is therefore possible to infer the influence they exert on the different molecular mechanisms. In this regard, the attention was focused on WRKYs, a family of TFs almost exclusively found in plant species. In grapevine, WRKYs are involved in several biological processes, playing a key role in berry development, hormonal balance and signalling, biotic and abiotic stresses responses, and secondary metabolites biosynthesis.