OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Grape and wine microorganisms: diversity and adaptation 9 Population-wide diversity study in Lachancea thermotolerans highlights superior starters for winemaking

Population-wide diversity study in Lachancea thermotolerans highlights superior starters for winemaking

Abstract

Grapes from warm(ing) climates often contain excessive sugars but lack acidity. This can lead to highly alcoholic wines with compromised stability and balance. The yeast Lachancea thermotolerans can ameliorate such wines due to its metabolic peculiarity – partial fermentation of sugars to lactic acid. This study aimed to elucidate the population-wide diversity in L. thermotolerans, whilst selecting superior strains for wine sector. An extensive collection of isolates (~200) sourced from different habitats worldwide was first genotyped on 14 microsatellite loci. This revealed differentiation of L. thermotolerans genetic groups based on the isolation substrate and geography. The 94 genotyped strains were then characterised in Vitis vinifera cv. Chardonnay fermentations. The comprehensive dataset comprised microbial growth and fermentation kinetics, primary metabolites and 90 volatile compounds. The common traits of L. thermotolerans strains were their glucophilic character, relatively extensive fermentation ability (>7.3 % v/v EtOH), low production of acetic acid and formation of lactic acid. A seven-fold variation was observed in concentrations of lactate (1.8 – 12 g/L), significantly affecting the wine pH (3.2 – 3.8). Besides the strain-derived variation (significant effect on 80/114 parameters), the metabolic dataset showed separation of pre-determined L. thermotolerans genetic groups. The superior L. thermotolerans strains were further evaluated in co-inoculations and sequential inoculations with Saccharomyces cerevisiae, required for fermentation completion. The chemical and sensory modulations in wines further highlighted the potential of L. thermotolerans strains to produce ‘fresher’ wines with lower ethanol content and improved flavour/balance.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Ana Hranilovic (1,2), Vladimir Jiranek (2, 3), Paul R. Grbin (2), Joanna M. Gambetta (4), Leigh Schmidtke (4), Paul K. Boss (5), Joana Coulon (6), Isabelle Masneuf-Pomarede (1,7), Marina Bely (1), Warren Albertin (1,8) 

1. Unité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de Bordeaux, Bordeaux INP, F33882 Villenave d’Ornon France 
2. Department of Wine and Food Science, The University of Adelaide, Adelaide, AU
3. The Australian Research Council Training Centre for Innovative Wine Production, Adelaide, AU
4. National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, AU 
5. CSIRO Agriculture and Food, Adelaide, AU 
6. Biolaffort, Floirac, FR 
7. Bordeaux Sciences Agro, Gradignan, FR 
8. ENSCBP, Bordeaux INP, Pessac, FR 

Contact the author

Keywords

Lachancea thermotolerans, non-Saccharomyces yeasts, population diversity ,wine composition

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

What defines the aging signature of Chasselas wines?

Chasselas is a refined grape variety renowned for its subtlety and its remarkable ability to reflect terroir characteristics [1]. Typically consumed young, it is appreciated for its low acidity and delicate fruity and floral aromas.

Red wine astringency and the influence of wine–saliva aggregates on oral lubrication

Oral tribology receives growing attention in the field of food sciences as it offers great opportunities to establish correlations between physical parameters, such as the coefficient of friction, and sensory perceptions in the human mouth.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

Grapevine genotypes with potential for reducing the carbon footprint in the atmosphere and cultivation in a biological system

The concentration of CO2 in the atmosphere is increasing from year to year. Taking into account the calculations of the greenhouse gas inventory, it was found that approximately 70% of CO2 in the atmosphere is absorbed by vegetation (forests, agricultural land, etc.).

Sustainablity of vineyards in the Priorat region (NE Spain)

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...