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IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2010 9 Impact of microbiological and enological techniques on wine macromolecules 9 Evolution of biogenic amines content in wine during sample conservation – method optimisation for analysis of biogenicamines

Evolution of biogenic amines content in wine during sample conservation – method optimisation for analysis of biogenicamines


The present paper reports the development of an optimized method for simultaneous analysis of 8 biogenic amines (Histamine, Methylamine, Ethylamine, Tyramine, Putrescine, Cadaverine, Phenethylamine, and Isoamylamine). It is based on a method developed by Gomez-Alonso et al. in 2007. The proposed analytical method has the following advantages: easy derivatization of wines, quantification of biogenic amines, and complete degradation of excess derivatization reagent during sample preparation to preserve column. It consists of reversed phase separation by HPLC and UV- vis detection of the aminoenones formed by the reaction of amino compounds with the derivatizationreagent diethyl ethoxymethylenemalonate (DEEMM). The technique was confirmed with an alternative oenological analysis method for the validation, quality control and uncertainty assessment (OIV Oeno 10/2005).

Publication date: July 3, 2024

Issue: Macrowine 2010

Type: Article


Benoît BACH1, Stéphanie LE QUERE1, Magali GRIMBAUM2, Patrick VUCHOT 1, Laurent BARNAVON 1

1 Inter Rhône, Service technique, 2260 route du Grès, F-84100 Orange, F.

2 Institut Français de la Vigne et du Vin, Pôle Rhône-Méditerranée, Institut Rhodanien, 2260, route du Grès F-84100 Orange, F.

Contact the author*


wine, biogenic amine, Rhône Valley


IVES Conference Series | Macrowine | Macrowine 2010


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