Conservation: the best valorisation strategy for wine growing areas

Over the past two years, the AWRI has received 69 wine samples suspected of being affected by mousy off-flavour. The character has been mostly observed in white wines.

In this work, results about the composition in polyphenols and polyamines in important wine-grape cultivars from the Emilia-Romagna region are presented. Spectrophotometric and HPLC analyses suggest that especially coloured berries are particularly rich of antioxidant species (stilbenes and catechins). Potential allergenic capability of biogenic amines was also characterized.

It is well documented that varietal aroma is an important parameter of wine quality. Chemical compounds responsible for wine varietal aroma are sourced from secondary grape metabolites. Until today little research is conducted on the influence of vine clone on the grape aromatic content of Greek grape varieties. Xinomavro (Vitis vinifera L.) is one of the most important Greek grape varieties, valuable for the wine industry of Northern Greece since it contributes to the production of PDO wine of Naoussa, Amindeo and Goumenissa.

The most recent methodological developments in soil survey and land evaluation, that can be taken as reference in the viticultural field, go over usage of the GIS and database. These informatic tools, which begin to be widely utilised, consent to realise evaluations at different geographic scale and with different data quality and quantity in entrance.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.