Terroir 2020 banner
IVES 9 IVES Conference Series 9 International Terroir Conferences 9 Terroir 2020 9 History and innovation of terroir 9 Using image analysis for assessing downy mildew severity in grapevine

Using image analysis for assessing downy mildew severity in grapevine

Abstract

Aim: Downy mildew is a crucial disease in viticulture. In-field evaluation of downy mildew has been classically based on visual inspection of leaves and fruit. Nevertheless, non-invasive sensing technologies could be used for disease detection in grapevine. The aim of this study was to assess downy mildew severity in grapevine leaves using machine vision.

Methods and Results: Leaf disks of the cv Pinot Noir (Vitis vinifera L.) were placed in Petri dishes with the abaxial side up. Plasmopara viticola sporangia were collected from infected leaves in the vineyard and used for the experimental inoculation of the leaf disks in laboratory. Images of Petri dishes including different levels of downy mildew infection were taken using a digital RGB camera. Machine vision techniques were used to estimate downy mildew severity (percentage of pixels representing visual symptoms) on the leaves. The symptoms were evaluated by eight experts, visually estimating the percentage of area showing sporulation. Considering the average evaluation of the experts, the assessment obtained by the new developed algorithm based on computer vision was represented as a R2value of 0.82 and RMSE of 14.34%.

Conclusions:

The results show a strong correlation between the severity computed by machine vision and the visual assessments, opening the possibility of the automated evaluation of downy mildew severity using non-invasive sensors.

Significance and Impact of the Study: The results indicated that machine vision can be applied for assessing and quantify visual symptoms of downy mildew in grapevine

DOI:

Publication date: March 23, 2021

Issue: Terroir 2020

Type: Video

Authors

Inés Hernández1, Salvador Gutiérrez2, Sara Ceballos1, Miriam Alonso1, Umberto Calvo1, Ignacio Barrio1, Fernando Palacios1, Silvia Toffolatti3, Giuliana Maddalena3, Javier Tardaguila1*

1Televitis Research Group. University of La Rioja, 26007 Logroño, Spain
2Department of Computer Science and Engineering, University of Cádiz, 11519 Puerto Real, Spain
3Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, 20133, Milano, Italy

Contact the author

Keywords

Grapevine, downy mildew, non-invasive phenotyping tools, imaging, machine vision

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Impact of the ‘Pinot’-family on early ripening in cool climate viticulture varieties

‘Pinot Precoce Noir’ (PPN) is an early ripening clone of ‘Pinot Noir’ (PN). The phenological differentiation is visible by an about two weeks earlier onset of veraison. It was found that the early veraison locus Ver1 on chromosome 16, previously identified in ‘Calardis Musqué’, originated from PPN. A highly correlated SSR marker, namely GF16-Ver1, was developed and tested for its ability to molecularly differentiate between PPN and PN as well as its potential to trace individual descendants.

Postveraison shoot trimming in Tannat and Merlot: preliminary results on yield components, plant balance and berry composition

There is currently a trend towards the production of wines with low alcohol content. To achieve this, grapes with low sugar content must be used. There are techniques at the vineyard level that can delay ripening and avoid excessive sugar accumulation without, a priori, affecting the final polyphenol content. Postveraison shoot trimming (PVST) is experimentally evaluated for these purposes, but its impact under Uruguayan climatic conditions with high interannual variability is not known. The aim of this work is to assess the PVST in Tannat and Merlot cultivars and their impact on yield components, plant balance and berry primary composition. In this study, two commercial vineyards of 10 years old Tannat and Merlot (grafted on SO4) at Canelones Department were selected. During the 2020-201 growing season, grapevines were submitted to PVST when grapes reached 15º Brix. In a randomized block, trimmed (T) and control (C) plants were evaluated with three repetitions each cultivar. Evaluation of the evolution of primary berry composition during ripening, measurement of yield components and plant balance were performed. For both cultivars, PVST did not affect yield components. Merlot reached 5.4 kg per plant and Tannat 7.1 kg, with not statistical significance between treatments. However, statistical differences were observed in terms of plant balance. In Merlot Ravaz Index reached a difference of 5.3 (12.0 in T and 6.7 in C) meanwhile Tannat reached 3.5 of statistical difference (13.7 in T and 10.2 in C). The tendency to imbalance for the treated plants had an impact on the final grape composition. Merlot grapes showed statistical difference in final total acidity (0.3 g of difference between treatments) while treatments impact final sugar content on Tannat grapes (10.0 g of difference between treatments). Further studies are needed to assess the impact of different canopy management techniques in our conditions.

Climat et sol: critères d’évaluation et effets sur le comportement de la vigne

Le zonage viticole aborde en premier lieu la caractérisation des macroclimats aux échelles des grandes régions, pays, continents ou monde (géoviticulture).

Evaluation of the effect of regulated deficit irrigation on Vitis vinifera Cabernet-Sauvignon physiological traits and final fruit composition

Climate change establishes challenges, as well as opportunities for many sectors, and markedly the wine sector.

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.