Terroir 2020 banner
IVES 9 IVES Conference Series 9 Plant nitrogen assimilation and partitioning as a function of crop load

Plant nitrogen assimilation and partitioning as a function of crop load

Abstract

Aims: The optimization of nitrogen use efficiency (NUE, i.e. uptake, assimilation and partitioning) is a solution towards the sustainable production of premium wines, while reducing fertilization and environmental impact. The influence of crop load on the accumulation of N compounds in fruits is still poorly understood. The present study assesses the impacts of bunch thinning on NUE and the consequences on the free amino N (FAN) profile in fruits.

Methods and Results: A large crop load gradient was imposed by bunch thinning (0.5 to 2.5 kg m–2) in a homogeneous plot of 225 vines. Isotope-labelled foliar urea (10 atom % 15N) was applied on the canopy of the fertilized treatment at veraison. The plants were excavated at four phenological stages over the two seasons (bud burst, flowering, veraison and harvest) and were individually split into five plant parts (roots, trunk, canopy, pomace and must). Total nitrogen and its stable isotope composition were determined in each part, with the aim of monitoring NUE as a function of crop load and fertilization.

The N concentration in fruits either at veraison or at harvest was not related to crop load variation. N concentration was maintained in the must to the detriment of N content in the roots. The root dry weight was 15 % lower and the root N quantity 27 % lower under high yielding conditions (HYC, compared to low yielding conditions LYC). The fertilizer N uptake was 41 % higher under HYC than under LYC. Consequently, urea supply had a positive impact on the yeast assimilable N concentration in the must (+55 mg L-1) only under HYC. However, the must FAN profile was significantly affected by the crop load, suggesting a possible modification of the aroma potential, independently from fertilization and grape maturation.

Conclusion: 

Using a 15N-labeling method, we demonstrate that grapevine has a strong ability to regulate nitrogen uptake and reserve mobilization to maintain a constant fruit N concentration despite changes in crop load. Foliar-urea fertilization at veraison was more efficient under HYC and helped to fulfill grape N demand, while limiting the mobilization of N reserves. However, the crop load affected the must FAN profile, inducing a possible modification of the fruit aroma. 

Significance and Impact of the Study: These findings highlight the great capacity of plants to adapt their N metabolism to constraints, e.g. bunch thinning in this case. These results are important to improve perennial fruit crop production through higher fertilization efficiency and lower environmental impact. Without fertilization, plant nutrition can be enhanced through the optimization of agricultural practices. The root activity appears to be key for understanding the mechanisms that balance N nutrition in plants

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Thibaut Verdenal1, Vivian Zufferey1, Agnes Dienes-Nagy1, Olivier Viret2, Cornelis van Leeuwen3, Jorge Spangenberg4, Jean-Laurent Spring1

1Agroscope Institute, Av. Rochettaz 21, CH-1009 Pully, Switzerland
2Direction générale de l’agriculture, de la viticulture et des affaires vétérinaires (DGAV), Av. de Marcelin 29, CH-1110 Morges, Switzerland
3EGFV, Bordeaux Sciences Agro, INRAE, Univ. Bordeaux, ISVV, F-33882 Villenave d’Ornon, France
4Institute of Earth Surface Dynamics, University of Lausanne, CH-1015 Lausanne, Switzerland

Contact the author

Keywords

Nitrogen partitioning, crop load, isotope labelling, amino acids, vines

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Effect of non-Saccharomyces yeast and lactic acid bacteria on selected sensory attributes and polyphenols of Syrah wines

Consumers predominantly use visual, aromatic and texture cues as quality/preference indicators to describe olfactory sensations. In this study, the effect of micro-organism in wine production was investigated using analytical and sensory techniques to achieve relevant analytical characterisation. Selected anthocyanins, flavan-3-ols, flavonols and phenolic acids were quantified in Syrah wines using RP-HPLC-DAD. Standard oenological parameters were also measured. Syrah grape must was fermented with various combinations of Saccharomyces cerevisiae (S. cerevisiae) and non-Saccharomyces (Metschnikowia pulcherrima or Hanseniaspora uvarum) yeasts, which was followed by sequential inoculation of lactic acid bacteria (LAB) (Oenococcus oeni or Lactobacillus plantarum).

Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.

Impact of aging on dimethyl sulfide (DMS) in Corvina and Corvinone wines

Amarone is an Italian red wine produced in the Valpolicella area, in north-eastern Italy. Due to its elaboration with withered grapes, Amarone is a rather unique example of dry red wine. However, there is very limited data so far concerning the volatile composition of commercial Amarone wines, which also undergo a cask aging of 2-4 years before release. The present work aims at characterizing the aroma composition of Amarone and to elucidate the relationships between chemical composition and sensory characters.

Partitioning of seasonal above‐ground biomass of four vineyard-grown varieties: development of a modelling framework to infer temperature-rate response functions

Aims: Forecasting the biomass allocation among source and sinks organs is crucial to better understand how grapevines control the distribution of acquired resources and has a great meaning in term of making decisions about agricultural practices in vineyards. Modelling plant growth and development is one of prediction approaches that play this role when it concerns growth rates in response to variation in environmental conditions

The generation of suspended cell wall material may limit the effect of ultrasound in some varieties

The disruptive effect exerted by high-power ultrasound (US) on plant cell walls, natural barriers to the diffusion of compounds of interest during the maceration of red wines, is established as the reason behind the chromatic improvement that its treatment causes. However, sometimes this improvement is not observed, especially with short maceration times. The presence of a high quantity of suspended cell wall material, which formation is favored by the sonication, could be the cause of this lack of positive results since this cell wall material has a high affinity for phenolic compounds.