Terroir 2020 banner
IVES 9 IVES Conference Series 9 Potential application of indigenous Pichia kluyveri for enhanced wine aroma quality

Potential application of indigenous Pichia kluyveri for enhanced wine aroma quality

Abstract

Aims: In previous work, five indigenous Pichia kluyveri strains, GS1-1, FS-2-7, HS-2-1, C730 and C732, were isolated and selected from spontaneous fermented wines from Ningxia and Gansu. The aims of this study were to 1) evaluate resistance of these strains to environmental stressors that may restrict their growth and the progress of alcoholic fermentation; 2) Investigate their fermentation dynamics; 3) Characterise aroma profiles of Cabernet Sauvignon wines made from mixed cultures of P. kluyveri and Saccharomyces cerevisiae

Methods and Results: Tolerance assays were conducted in YEPD medium to test resistance of each Pichia kluyveri strain to sugar, pH, ethanol, temperature and free SO2. All strains except FS-2-7 were able to tolerate 60% w/v glucose, low pH of 2.0, 16% v/v ethanol, extreme fermentation temperatures (11˚C and 44˚C), and 500 mg/L total SO2. Following this, these strains were inoculated into a synthetic grape juice medium to test their fermentation performance and evaluate basic parameters of the final synthetic wine. Strain HS-2-1 was the first to initiate fermentation, and produced significantly higher amounts of total organic acids and less volatile acids compared to other strains. Thus, strain HS-2-1 was chosen for further characterisation in Cabernet Sauvignon fermentation trials co-fermented with S. cerevisiae NX11424 at different ratios. Viable yeast cell numbers were determined by plate counting. Yeast-derived volatile compounds of the final wine were analysed using head space-solid phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC/MS). Mixed fermentation did not inhibit HS-2-1 growth, and also produced less volatile acid, and significantly more esters and higher alcohols compared to single fermentation by S. cerevisiae. Notably, concentrations of isopentanol, ethyl butyrate, ethyl hexanoate, ethyl octanoate, ethyl 9-decenoate and ethyl lactate increased in line with the increased proportion of HS-2-1 in the inoculum.

Conclusions: 

This study shows indigenous P. kluyveri HS-2-1 has good resistance to alcoholic fermentation associated common stressors, better fermentation performance, and excellent oenological characteristics when co-fermenting with S. cerevisiae

Significance and Impact of the Study: Chinese wine regions such as Ningxia and Gansu have developed dramatically in recent years. These wine regions are in great need to produce wines with typical regional characteristics. To promote regional typicity, using selected indigenous yeasts could introduce a unique local character or “terroir” during winemaking. Pichia kluyveri widely occurs at earlier stages of spontaneous fermentation, however limited research has been done on its oenological characteristics. This study comprehensively investigated the features of indigenous P. kluyveri strain(s), and highlighted the potential application of strain HS-2-1 in winemaking by co-fermenting with S. cerevisiae for improving the fruity and floral aroma profile of these Chinese wines.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type: Video

Authors

iao Jiang1, Wenjing Zhang1, Li Feng1, Dongqing Ye1, Yanlin Liu1,2*

1College of Enology, Northwest Agricultural and Forestry University, Yangling, Shaanxi 712100, China
2Shaanxi Engineering Research Center for Viti-viniculture, Yangling, Shaanxi 712100, China

Contact the author

Keywords

Pichia kluyveri, stress tolerance, fermentation, volatile compounds, aroma  

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Impact of changes in pruning practices on vine growth and yield

A gradual decline in vineyards has been observed over the past twenty years worldwide. This might be explained by the climate change, practices change or the increase of dieback diseases. To increase the longevity of vines, we studied the impact of different pruning strategies in four adult and four young vineyards located in France and Spain. In France, vineyards were planted with Cabernet franc on 3309C while Spanish trials were planted with Tempranillo grafted on 110R. Vegetative expression, yield, quality of berries and wood vessels conductivity were measured. The distribution of vegetative expression, yield and berry composition between primary and secondary vegetation were quantified. Finally, tomography was used to evaluate the implication of the treatments on sap flows.
First results show that i) the respectful pruning leads to an increase of 30 to 50% more secondary shoots than the aggressive pruning in France and between 15 and 20% in Spain, ii) there is no major effect on the yield over the first two years following the implementation of the new pruning practices, although the proportion of clusters from suckers is higher on the respectful pruning method. On young vines, the development of the trunk according to a respectful pruning leads to a loss of harvest 2 years after planting. This is due to the removal, on the future trunk, of the green suckers which carrying bunches. This operation carried out in spring rather than during winter pruning, would promote a better leaf / fruit balance when the plant comes into production, and could lead to better hydraulic conduction in the vessels of the trunk. Maintaining these trials for several years will provide more robust data to assess the impact of these practices on the vines over the long term.

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…