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IVES 9 IVES Conference Series 9 OIV 9 OIV 2024 9 Orals - Oenology, methods of analysis 9 Development of a standardized method for metabolite analysis by NMR to assess wine authenticity

Development of a standardized method for metabolite analysis by NMR to assess wine authenticity

Abstract

The wine sector generates a considerable amount of wealth but is facing a perpetual problem of fraud. Wine counterfeiting is one of the oldest and most common cases of food fraud worldwide. Therefore, the authenticity and traceability of wine are major concerns for both industry and consumers. To address these issues, robust and reliable analysis and control methods are necessary. Several methods have been developed, ranging from simple organoleptic tests to more advanced methodologies such as isotopic techniques or residual radioactivity measurements. However, with counterfeiting becoming increasingly sophisticated, more complex methodologies are needed for their detection. In this context, innovative tools for the analysis of metabolites and other small organic molecules can offer new perspectives for ensuring wine authenticity. In this work, we propose the development and validation of an open metabolomic approach based on 1H NMR to assess wine authenticity, using standardized, flexible, and accessible analytical and data processing protocols. Additionally, combinatorial approaches using orthogonal techniques e.g. NMR, high-resolution mass spectrometry, and data fusion are presented. These efforts represent a significant advancement towards establishing a standardized and open methodology for certifying wine authenticity using 1H NMR metabolomics and establish new metabolomic approaches for wine authentication and quality control.

Desarrollo de un método estandarizado mediante análisis metabolómico RMN para garantizar la autenticidad del vino

El sector vitivinícola genera una cantidad considerable de riqueza pero enfrenta un problema creciente de fraude. La falsificación de vinos es uno de los casos más antiguos y comunes de fraude alimentario en todo el mundo. Por lo tanto, la autenticidad y trazabilidad del vino son preocupaciones importantes tanto para la industria como para los consumidores. Para abordar estos problemas, son necesarios métodos de análisis y control robustos y confiables. Se han desarrollado varios métodos, que van desde simples pruebas organolépticas hasta metodologías más avanzadas como técnicas isotópicas o mediciones de radiactividad residual. Sin embargo, con la falsificación volviéndose cada vez más sofisticada, se necesitan metodologías más complejas para su detección. En este contexto, las aplicaciones innovadoras de herramientas metabolómicas pueden ofrecer nuevas perspectivas para garantizar la autenticidad del vino.  En este trabajo, proponemos el desarrollo y validación de un método metabolómico abierto basado en rmn 1h para garantizar la autenticidad del vino, utilizando protocolos estandarizados, flexibles y accesibles. Estos esfuerzos representan un avance significativo hacia el establecimiento de una metodología estandarizada y abierta para certificar la autenticidad del vino utilizando metabolómica de rmn 1h.  Además, se están explorando otros métodos, como la combinación de análisis por rmn, espectrometría de masas de alta resolución y fusión de datos. Este estudio abre el camino para nuevos métodos metabolómicos para la autenticación y el control de calidad del vino.

Développement d’une méthode standardisée par analyse métabolomique RMN pour garantir l’authenticité des vins

Le secteur viticole génère une grande quantité de richesse, mais doit faire face à un problème croissant de fraudes. La contrefaçon des vins est l’un des cas les plus anciens et les plus courants de fraude alimentaire dans le monde. Par conséquent, l’authenticité et la traçabilité du vin sont des enjeux majeurs pour la filière et les consommateurs. Pour répondre à ces enjeux, il est nécessaire de disposer de méthodes d’analyse et de contrôle robustes et fiables. Plusieurs méthodes ont été développées, allant de simples tests organoleptiques à des méthodologies plus avancées telles que les techniques isotopiques, ou les mesures de radioactivité résiduelle. Cependant, avec des contrefaçons devenant de plus en plus sophistiquées, des méthodologies plus complexes sont nécessaires pour leur détection. Dans ce contexte, des applications innovantes des outils de la métabolomique peuvent offrir de nouvelles perspectives pour assurer l’authenticité du vin.   Dans ce travail, nous proposons le développement et la validation d’une approche métabolomique ouverte basée sur la rmn 1h pour garantir l’authenticité du vin, en utilisant des protocoles standardisés flexibles et accessibles. Ces travaux représentent une avancée significative vers l’établissement d’une méthodologie normalisée et ouverte pour la certification de l’authenticité d’un vin en utilisant la métabolomique rmn 1h.   De plus, d’autres approches sont en cours d’exploration comme la combinaison des analyses par rmn, spectrométrie de masse à haute résolution et fusion de données. Cette étude ouvre la voie à de nouvelles approches métabolomiques pour l’authentification et le contrôle de la qualité du vin.

DOI:

Publication date: November 18, 2024

Issue: OIV 2024

Type: Article

Authors

Guillaume Leleu1, Patricia Homobono Brito de Moura1,2, Grégory Da Costa1,2, Cléo Berrehail2,3, Jean-Claude Boulet4,5, Rémi Butelle1, Catherine Deborde2,3#, Matthieu Dubernet6, Laetitia Gaillard7, Antoine Galvan7, Karen Gaudin1,8, Alexandra Gossé9, Markus Herderich10,11,12, Daniel Jacob2,3#, Christine Le Guernevé4, Elodie Milani Crouzet4,5, Annick Moing2,3, Sophie Rosset7, Josep Valls-Fonayet1,2, Flynn Watson11,12, Tristan Richard1,2

1 Univ. Bordeaux, Bordeaux INP, INRAE, Bordeaux Sciences Agro, OENO, UMR 1366, ISVV, F-33140 Villenave-d’Ornon, France
2 MetaboHUB-Bordeaux, F-33140 Villenave-d’Ornon, France
3 INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave-d’Ornon, F-33140, France
4 INRAE, Institut Agro Montpellier, Univ. Montpellier, SPO, UMR 1083, F-34060 Montpellier, France
5 INRAE, PROBE research infrastructure, PFP polyphenols analysis facility, F-34060 Montpellier, France
6 Laboratoire Dubernet, 35 rue de Combe du Meunier, 11100 Montredon-des-Corbières, France
7 Service Commun des Laboratoires, 3 avenue du Dr. Albert Schweitzer, F-33600 Pessac, France
8 Univ. Bordeaux, Laboratoire de Chimie Analytique, F-33000, Bordeaux, France
9 Les Grands Chais De France, 1 rue de la Division Leclerc, F-67290 Petersbach, France
10 The University of Adelaide, Waite Research Institute, Urrbrae, 5064, SA, Australia
11 The Australian Wine Research Institute (AWRI), Urrbrae, 5064, SA, Australia
12 Metabolomics Australia, Urrbrae, 5064, SA, Australia
# Actual address: INRAE, UR 1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France, INRAE, PROBE Research Infrastructure, BIBS facility, Institute, F-44300 Nantes, France

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Full papers OIV 2024 | IVES Conference Series | OIV | OIV 2024

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