Terroir 2020 banner
IVES 9 IVES Conference Series 9 Terpenoid profiles and biosynthetic gene expression pattern in Asti DOCG white muscat grapes at ripening as affected by different canopy management protocols

Terpenoid profiles and biosynthetic gene expression pattern in Asti DOCG white muscat grapes at ripening as affected by different canopy management protocols

Abstract

Aim: The main goal of this study was to find an efficient canopy management to limit the high temperature-related aroma losses of White Muscat grapes, and consequently to preserve the quality standards of Asti DOCG wines.

Methods and Results: Four different strategies have been tested in two vineyards of the Asti DOCG production area: pre-flowering leaf removal (m1), post-berry set leaf removal (m2), leaf removal at veraison (m3), and clusters thinning (m4). Control vines (m0) did not receive any thinning or defoliation. Grapes were collected at four time points: seven days before the commercial harvest, at the commercial harvest scheduled for “Asti spumante” wine, at the commercial harvest scheduled for “Moscato” wine and overripening. Free and glycosylated terpenoids content (GC-MS) as well as the expression of key genes involved in terpenoids biosynthesis and metabolism (RT-qPCR) were analysed separately in skin and pulp. The results revealed a peak of volatile accumulation, which occurred early and late throughout the sampling times. The treatments m3 and m4 were, in general, those more effective in enhancing the aroma profiles in both tissues analysed. Correspondingly, in these grapes, specific genes, such as VvDXS3 and VvGT14 resulted up-regulated. Other genes, such as VvHDR, showed different expression pattern resulting, in general, more expressed in pulp than skin, regardless the applied treatment.

Conclusions:

Based on these preliminary trials carried out in a specific production area of White Muscat, it seems that m3 and m4 treatments had a significant effect on the volatile’s accumulation in both grape skin and pulp. m1 treatment resulted to be the less effective in inducing changes in the aroma profile and the terpenoid biosynthetic pathway.

Significance and Impact of the Study: Moscato d’Asti DOCG is one of the most characteristic enological products of Piemonte (North-West Italy) wine grapes-growing area. It comes exclusively from White Muscat grapes which are exalted by the climatic and geographical conditions of the production area. Indeed, the interactions between vine and environment, limestone terrain and micro-climate typical of hilly zones leads to a characteristic fruity and sweety aroma. The characteristic aroma of Muscat wine is attributed to the presence of specific terpenoids, mainly linalool, nerol, geraniol, trans-piran linalool oxide and citronellol. The grapevine terpenoids pathway is strongly regulated by endogenous and environmental factors and among them, temperature and light exposure plays a crucial role. As recently observed, the content of these compounds is strongly decreasing due to the increasing temperatures. Higher temperature during the growing season is forcing growers to find ways to reliably control grape composition preserving the typical aroma of Asti DOCG wines. The present study could offer important information to address grower’s choice in term of canopy management that are better suited to the changing climate.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Margherita Modesti1*, Ron Shmulevitz, Stefano Brizzolara1, Daniele Eberle2, Guido Bezzo2, Pietro Tonutti1

1Life Sciences Institute, Scuola Superiore Sant’Anna, Piazza Martiri della Libertà 33, 50127 Pisa, Italy
2Consorzio per la Tutela dell’Asti DOCG. Piazza Roma 10, 14100 Asti, Italy

Contact the author

Keywords

Canopy management, Moscato d’Asti DOCG, terpenoid content and biosynthesis, climate change

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Local adaptation tools to ensure the viticultural sustainability in a changing climate

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

A multidisciplinary approach to evaluate the effects of the training system on the performance of “Aglianico del Vulture” vineyards

Vineyards are complex agro-ecosystems with high spatial and temporal variability. An efficient training system may counteract the adverse effects of this variability. Moreover, considering the climate change issues, choosing an efficient training system that enhances water use and protects the vines from radiative thermal stress has become a priority for the farmers. A multidisciplinary approach that assesses the soil-crop-yield-wine relationships of vineyards in a distributed and holistic way could bring added knowledge on the behavior of the different training systems. This ongoing research aimed to implement a multidisciplinary approach to study the behavior of “Aglianico del Vulture” grapevines trained with two different systems: a spurred cordon (SC) and an “Alberello in parete” (AL), grown in a high-quality wine production area of Basilicata region (Italy). The approach merged several methods and scales of soil, ecophysiology, must/wine quality, and spectral data collection to assess the influence of the training system. Homogeneous zones (HZs) in both training systems were defined through a procedure based on geomorphological classification, unmanned aerial vehicles (UAV) images analysis, and a traditional soil survey supported by geophysical scanning. During the 2021 season, TDR probes monitored soil water content, while grapevine health status was assessed using eco-physiological measurements (LWP, chlorophyll content, PSII photosynthetic efficiency, LAI, and point-based field spectroscopy). These grapevine in-vivo measurements validated the spectral vegetation indexes (NDVI, RENDVI, CVI, and TVI) derived from the UAV multispectral imagery, which monitored the grapevine status in a distributed and non-invasive way. Grape yield, quality of berries, must and wine were measured to assess the effects of the training systems. The first experimental year results showed the variability of the vineyards and revealed relationships among soil parameters, crop characteristics, and vegetation indices of the SC and AL training systems. This multidisciplinary study could bring new insights into the vineyard training system’s effects on grape yield and wine quality.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.

Mechanisms involved in the heating of the environment by the aerodynamic action of a wind machine to protect a vineyard against spring frost

One of the main consequences of global warming is the rise of the mean temperature. Thus, the heat summation by the plants begins sooner in the early spring, and by cumulating growing degree-days, phenological development tends to happen earlier. However, spring frost is still a recurrent phenomenon causing serious damages to buds and therefore, threatening the harvests of the winegrowers. The wind machine is a solution to protect fruit crops against spring frost that is increasingly used. It is composed of a 10-m mast with a blowing fan at its peak. By tapping into the strength of the nocturnal thermal inversion, it sweeps the crop by propelling warm air above to the ground. Thus, stratification is momentarily suppressed. Furthermore, the continuous action of the machine, alone or in synergy, or the addition of a heater allow the bud to be bathed in a warmer environment. Also, the punctual action of the tower’s warm gust reaches the bud directly at each rotation period. All these actions allow the bud to continuously warm up, but with different intensities and over a different period. Although there is evidence of the effectiveness of the wind machines, the thermal transfers involved in those mechanisms raise questions about their true nature. Field measurements based on ultrasonic anemometers and fast responding thermocouples complemented by laboratory measurements on a reduced scale model allow to characterize both the airflow produced by the wind machine and the local temperature in its vicinity. Those experiments were realized in the vineyard of Quincy, in the framework of the SICTAG project. In the future paper, we will detail the aeraulic characterization of the wind machine and the thermal effects resulting from it and we will focus on how the wind machine warms up the local atmosphere and enables to reduce the freezing risk.