Terroir 2020 banner
IVES 9 IVES Conference Series 9 Terpenoid profiles and biosynthetic gene expression pattern in Asti DOCG white muscat grapes at ripening as affected by different canopy management protocols

Terpenoid profiles and biosynthetic gene expression pattern in Asti DOCG white muscat grapes at ripening as affected by different canopy management protocols

Abstract

Aim: The main goal of this study was to find an efficient canopy management to limit the high temperature-related aroma losses of White Muscat grapes, and consequently to preserve the quality standards of Asti DOCG wines.

Methods and Results: Four different strategies have been tested in two vineyards of the Asti DOCG production area: pre-flowering leaf removal (m1), post-berry set leaf removal (m2), leaf removal at veraison (m3), and clusters thinning (m4). Control vines (m0) did not receive any thinning or defoliation. Grapes were collected at four time points: seven days before the commercial harvest, at the commercial harvest scheduled for “Asti spumante” wine, at the commercial harvest scheduled for “Moscato” wine and overripening. Free and glycosylated terpenoids content (GC-MS) as well as the expression of key genes involved in terpenoids biosynthesis and metabolism (RT-qPCR) were analysed separately in skin and pulp. The results revealed a peak of volatile accumulation, which occurred early and late throughout the sampling times. The treatments m3 and m4 were, in general, those more effective in enhancing the aroma profiles in both tissues analysed. Correspondingly, in these grapes, specific genes, such as VvDXS3 and VvGT14 resulted up-regulated. Other genes, such as VvHDR, showed different expression pattern resulting, in general, more expressed in pulp than skin, regardless the applied treatment.

Conclusions:

Based on these preliminary trials carried out in a specific production area of White Muscat, it seems that m3 and m4 treatments had a significant effect on the volatile’s accumulation in both grape skin and pulp. m1 treatment resulted to be the less effective in inducing changes in the aroma profile and the terpenoid biosynthetic pathway.

Significance and Impact of the Study: Moscato d’Asti DOCG is one of the most characteristic enological products of Piemonte (North-West Italy) wine grapes-growing area. It comes exclusively from White Muscat grapes which are exalted by the climatic and geographical conditions of the production area. Indeed, the interactions between vine and environment, limestone terrain and micro-climate typical of hilly zones leads to a characteristic fruity and sweety aroma. The characteristic aroma of Muscat wine is attributed to the presence of specific terpenoids, mainly linalool, nerol, geraniol, trans-piran linalool oxide and citronellol. The grapevine terpenoids pathway is strongly regulated by endogenous and environmental factors and among them, temperature and light exposure plays a crucial role. As recently observed, the content of these compounds is strongly decreasing due to the increasing temperatures. Higher temperature during the growing season is forcing growers to find ways to reliably control grape composition preserving the typical aroma of Asti DOCG wines. The present study could offer important information to address grower’s choice in term of canopy management that are better suited to the changing climate.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Margherita Modesti1*, Ron Shmulevitz, Stefano Brizzolara1, Daniele Eberle2, Guido Bezzo2, Pietro Tonutti1

1Life Sciences Institute, Scuola Superiore Sant’Anna, Piazza Martiri della Libertà 33, 50127 Pisa, Italy
2Consorzio per la Tutela dell’Asti DOCG. Piazza Roma 10, 14100 Asti, Italy

Contact the author

Keywords

Canopy management, Moscato d’Asti DOCG, terpenoid content and biosynthesis, climate change

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

Leaf vine content in nutrients and trace elements in La Mancha (Spain) soils: influence of the rootstock

The use of rootstock of American origin has been the classic method of fighting against Phylloxera for more than 100 years. For this reason, it is interesting to establish if different rootstock modifies nutrient composition as well as trace elements content that could be important for determining the traceability of the vine products. A survey of four classic rootstocks (110-Richter, SO4, FERCAL and 1103-Paulsen) and four new ones (M1, M2, M3 and M4) provided by Agromillora Iberia. S.L.U., all of them grafted with the Tempranillo variety, has been carried out during 2019. The eight rootstocks were planted in pots of 500 cc, on three soils with very different characteristics from Castilla-La Mancha (Spain). In the month of July, the leaves were collected and dried in a forced air oven for seven days at 40ºC. Then, the samples were prepared for the analysis determination, carried out by X-Ray fluorescence spectrometry. The results obtained showed that in the case of content in mineral elements in leaf, separated by soil type, we can report the importance of few elements such as Si, Fe, Pb and, especially, Sr. The rootstock does not influence the composition of the vine leaf for the studied elements that are the most important in determining the geochemical footprint of the soil. The influence of the soil can be discriminated according to some elements such as Fe, Pb, Si and, especially, Sr.

Assessment of the impact of actions in the vineyard and its surrounding environment on biodiversity in Rioja Alavesa (Spain)

Traditional viticulture areas have experienced in the last decades an intensification of field practices, linked to an increased use of fertilisers and phytosanitary products, and to a more intensive mechanization and uniformization of the landscape. This change in management has sometimes led to higher rates of soil erosion andloss of soil structure, fertility decline, groundwater contamination, and to an increased pressure of pests and diseases. Additionally, intensification usually leads to a simplification of landscapes, of particular concern in prestigious wine grape regions where the economical revenue encourages the conversion of land use from natural habitats to high value wine grape production. To revert this trend, it is necessary that growers implement actions that promote biodiversity in their vineyards. The aim of this study is to assess the impact of the implementation of cover crops, vegetational corridors, dry stone walls and vineyard biodiversity hotspots estimated through the study of arthropods. The work has been carried out in four vineyards in Rioja Alavesa belonging to Ostatu winery, where these infrastructures were implemented in 2020. The presence and diversity of arthropods was studied by capturing them at different times in the season and at different distances from the infrastructure using pit-fall traps in the soil and yellow, white and blue chromatic traps at the canopy level. This is a preliminary study in which all adult insects were sorted to the taxonomic level of order and Coleoptera were classified to morphospecies. The results obtained show that there is a relationship between the basic characteristics of the vineyard and the arthropods captured, with a positive effect, although also dependent on the vineyard, of the presence of infrastructure.

Traditional agroforestry vineyards, sources of inspiration for the agroecological transition of viticulture

A unique “terroir” can be found in southern Bolivia, which combines the specific features of climate, topography and altitude of high valleys, with the management of grapevines staked on trees. It is one of the rare remnants of agroforestry viticulture. A survey was carried out among 29 grapegrowers in three valleys, to characterize the structure and management of these vineyards, and identify the services they expect from trees. Farms were small (2.2 ha on average) and 85% of vineyards were less than 1 ha. Viticulture was associated with vegetable, fruit and fodder production, sometimes in the same fields. Molle trees were found in all plots, together with one or two other native tree species. Traditional grapevine varieties such as Negra Criolla, Moscatel de Alejandría and Vicchoqueña were grown with a large range of densities from 1550 to 9500 vines ha-1. From 18 to 30% of them were staked on trees, with 1.2 to 4.9 vines per tree. The management of these vineyards (irrigation, fertilization and grapevine protection) was described, the most particular technical operation being the coordinated pruning of trees and grapevines. Three types of management could be identified in the three valleys. Grapegrowers had a clear idea of the ecosystem services they expected from trees in their vineyards. The main one was protection against climate hazards (hail, frost, flood). Then they expected benefits in terms of pest and disease control, improvement of soil fertility and resulting yield. At last, some producers claimed that tree-staking was quicker and cheaper than conventional trellising. It can be hypothesized then that agroforestry is a promising technique for the agroecological transition of viticulture. Its contribution to the “terroir” of the high valleys of southern Bolivia and its link with the specificities of the wines and spirits produced there remain to be explored.

Differential responses of red and white grape cultivars trained to a single trellis system – the VSP

Commercial grape production relies on training grapevine cultivars onto a variety of trellis systems. Training allows for well-lit leaves and clusters, maximizing fruit quality in addition to facilitating cultivation, harvesting, and diseases control. Although grapevines can be trained onto an infinite variety of trellis systems, most red and white cultivars are trained to the standard VSP (Vertical Shoot Positioning) system. However, red and white cultivars respond differently to VSP in fruit composition and growth characteristics, which are yet to be fully understood. Therefore, the objective of this study was to examine the influence of the VSP trellis system on fruit composition of three red, Cabernet Sauvignon, Merlot and Syrah, and three white, Chardonnay, Riesling, and Gewurztraminer cultivars grown under uniform growing conditions in the same vineyard. All cultivars were monitored for maturity and harvested at their physiologically maximum possible sugar concentration to compare various fruit quality attributes such as Brix, pH, TA, malic and tartaric acids, glucose and fructose, potassium, YAN, and phenolic compounds including total anthocyanins, anthocyanin profile, and tannins. A distinct pattern in fruit composition was observed in each cultivar. In regards to growth characteristics, Syrah grew vigorously with the highest cluster weight. Although all cultivars developed pyriform seeds, the seed size and weight varied among all cultivars. Also varied were mesocarp cell viability, brush morphology, and cane structure. This knowledge of the canopy architectural characteristics assessed by the widely employed fruit compositional attributes and growth characteristics will aid the growers in better management of the vines in varied situations.