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IVES 9 IVES Conference Series 9 Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach


Aim: This study aimed to evaluate whether irrigation of Touriga Nacional in Douro Demarcated Region (DDR) can partly mitigate the negative impacts of ongoing climate change on grapevine yield and quality and its impact on plant metabolism.

Methods and results: Water status was determined by performing pre-dawn leaf water potential (ΨPd) using a pressure chamber throughout the growing season. Although from the end of July till the end of August of 2018 both R30 and R70 significantly prevented a decay of ΨPd under extreme drought conditions, R30 promoted only a relatively small increase of yield at harvest, but this increase was not observed at R70. In 2019, drought conditions were not so harsh than in 2018 and differences in cluster weights were not observed among irrigation treatments at harvest. A UPLC-MS-based targeted metabolomic analysis from the vintage 2018 identified 44 compounds in grapes from non-irrigated (R0), irrigated at 30% of evapotranspiration (ETc; R30) and 70% ETc (R70), corresponding to eight classes: amino acids; phenolic acids; stilbenoid DP1; stilbenoid DP2; flavonols; flavan-3-ols; di-OH anthocyanins and tri-OH anthocyanins. PCA analysis showed that irrigation influenced the composition of the different classes of grape berry compounds e.g. amino acids, phenolic acids, stilbenoids, flavonols, flavan-3-ols, and anthocyanins.


In the two consecutive seasons of 2018 and 2019 in DDR irrigation at R30 and R70 failed to bring Touriga Nacional vines to hydric comfort at veraison, when drought stress was more pronounced, and did not substantially affect yield and berry quality traits at harvest. However, UPLC-MS-base metabolomics analyses highlighted that berry metabolism was tuned under different irrigation regimes, but more water did not traduce in higher contents of key metabolites like anthocyanins

Significance and Impact of the Study: Douro Demarcated Region (DDR) has a Mediterranean climate with low rainfall values during summer, high temperatures and high levels of radiation. The introduction of irrigation in this region is still a matter of debate due to the limited number of available studies.


Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video


Inês Cabral1*, António  Teixeira2, Arnaud  Lanoue3, Marianne  Unlubayir3, Thibaut  Munsch3, Joana  Valente4, Fernando  Alves4, Pedro  Costa4, Frank  Rogerson4, Susana  Carvalho1, Hernâni  Gerós2,5,6, Anabela  Carneiro1, Jorge  Queiroz1

1GreenUPorto – Research Centre for Sustainable Agrifood Production & DGAOT, Faculty of Sciences, University of Porto, Vairão, Portugal 
2Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Gualtar, Portugal 
3Université de Tours, EA2106 Biomolécules et Biotechnologie Végétales, Tours, France 
4Symington Family Estates, Vila Nova de Gaia, Portugal 
5Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal 
6Centre of Biological Engineering (CEB), Department of Biological Engineering, University of Minho, Gualtar, Portugal

Contact the author


Deficit irrigation, metabolomics, leaf water potential, grape quality


IVES Conference Series | Terroir 2020


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