Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 Dual mode of action of grape cane extracts against Botrytis cinerea

Dual mode of action of grape cane extracts against Botrytis cinerea

Abstract

Crude extracts of Vitis vinifera canes represent a natural source of stilbene compounds with well characterized antifungals properties. In our trials, exogenous application of a stilbene extract (SE) obtained from grape canes on grapevine leaves reduces the necrotic lesions caused by Botrytis cinerea. The SE showed to possess a direct antifungal activity by inhibiting the mycelium growth. The activation of some grapevine defense mechanism was also investigated. H2O2 production and activation of mitogen-activated protein kinase (MAPK) phosphorylation cascades as well as accumulation of stilbenoid phytoalexins were explored on grapevine cell suspension. Moreover, the transcription of genes encoding for proteins affecting defense responses was analyzed on grapevine plants. The SE induced some grapevine defense mechanisms including MAPK activation, and the expression of pathogenesis-related (PR) genes and of a gene encoding the glutathione-S-transferase 1 (GST1). By contrast, treatment of grapevine leaves with SE negatively regulates de novo stilbene production.

References

Section for all references

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Gicele Sbardelotto De Bona1,2*, Marielle Adrian4, Jonathan Negrel4, Annick Chiltz4, Agnès Klinguer4, Benoît Poinssot4, Marie-Claire Héloir4, Elisa Angelini3, Simone Vincenzi1,2*, Nadia Bertazzon3

1Università di Padova, Dipartimento Territorio e Sistemi Agro-Forestali (TESAF), Viale dell’Università, 16, 35020 Legnaro (PD), Italia
2 Università di Padova, Dipartimento di Agronomia, Animali, Alimenti, Risorse naturali e Ambiente (DAFNAE), Viale dell’Università, 16, 35020 Legnaro (PD), Italia
3Centro di Ricerca Viticoltura ed Enologia (CREA), Via XXVIII Aprile 26, 31015 Conegliano, TV, Italia
4Agroécologie, AgroSup Dijon, CNRS, INRA, Université de Bourgogne, Franche- Comte, F-21000, Dijon, Francia

Contact the author

Keywords

cane extract, resveratrol, stilbenes, phytoalexin, Botrytis cinerea, grapevine, defense response

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

The impact of grazing by cattle on Vitis vinifera L. cv. Shiraz vegetative growth and metabolite profile

Context and purpose of the study. Globally, vineyard cultivation uses conventional methods to manage pests, diseases and increase yield.

The impact of postharvest cooling of Sauvignon blanc grapes on the sensory profile and the chemical composition of the wines

Rapid processing of grapes after harvest has always been considered essential for achieving a balanced sensory wine profile.

Identification and evaluation of the winemaking sub-zones of the PDO Amyndeo winegrowing region

Context and purpose of the study. The concept of terroir encompasses the investigation of the physical environment’s influence on grapevine physiology, grape composition, and wine quality, with an emphasis on employing viticultural zoning techniques to systematically characterize and analyze terroirs.

Searching for the sweet spot: a focus on wine dealcoholization

It is well known that the vinification of grapes at full maturation can produce rich, full-bodied wines,
with intense and complex flavour profiles. However, the juice obtained from such grapes may have very
high sugar concentration, resulting in wines with an excessive concentration of ethanol. In addition, the decoupling between technological maturity and phenolic/aromatic one due to global warming, exacerbates this problem in some wine-growing regions. In parallel with the increase of the mean alcohol content of wines on the market, also the demand for reduced alcohol beverages has increased in recent years, mainly as a result of health and social concerns about the risks related to the consumption of alcohol.

Effect of soil particle size on vine water status, leaf abscisic acid content and berry quality in nebbiolo grapes

AIM: We investigated the effect of soil texture on grapevine response to water stress, leaf abscisic acid concentration and berry quality, in two adjacent vineyards located in the renewed Cannubi hill of Barolo (Langhe area, CN, North-West Italy).