Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 Dual mode of action of grape cane extracts against Botrytis cinerea

Dual mode of action of grape cane extracts against Botrytis cinerea

Abstract

Crude extracts of Vitis vinifera canes represent a natural source of stilbene compounds with well characterized antifungals properties. In our trials, exogenous application of a stilbene extract (SE) obtained from grape canes on grapevine leaves reduces the necrotic lesions caused by Botrytis cinerea. The SE showed to possess a direct antifungal activity by inhibiting the mycelium growth. The activation of some grapevine defense mechanism was also investigated. H2O2 production and activation of mitogen-activated protein kinase (MAPK) phosphorylation cascades as well as accumulation of stilbenoid phytoalexins were explored on grapevine cell suspension. Moreover, the transcription of genes encoding for proteins affecting defense responses was analyzed on grapevine plants. The SE induced some grapevine defense mechanisms including MAPK activation, and the expression of pathogenesis-related (PR) genes and of a gene encoding the glutathione-S-transferase 1 (GST1). By contrast, treatment of grapevine leaves with SE negatively regulates de novo stilbene production.

References

Section for all references

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Gicele Sbardelotto De Bona1,2*, Marielle Adrian4, Jonathan Negrel4, Annick Chiltz4, Agnès Klinguer4, Benoît Poinssot4, Marie-Claire Héloir4, Elisa Angelini3, Simone Vincenzi1,2*, Nadia Bertazzon3

1Università di Padova, Dipartimento Territorio e Sistemi Agro-Forestali (TESAF), Viale dell’Università, 16, 35020 Legnaro (PD), Italia
2 Università di Padova, Dipartimento di Agronomia, Animali, Alimenti, Risorse naturali e Ambiente (DAFNAE), Viale dell’Università, 16, 35020 Legnaro (PD), Italia
3Centro di Ricerca Viticoltura ed Enologia (CREA), Via XXVIII Aprile 26, 31015 Conegliano, TV, Italia
4Agroécologie, AgroSup Dijon, CNRS, INRA, Université de Bourgogne, Franche- Comte, F-21000, Dijon, Francia

Contact the author

Keywords

cane extract, resveratrol, stilbenes, phytoalexin, Botrytis cinerea, grapevine, defense response

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

Grapevine genotypes with potential for reducing the carbon footprint in the atmosphere and cultivation in a biological system

The concentration of CO2 in the atmosphere is increasing from year to year. Taking into account the calculations of the greenhouse gas inventory, it was found that approximately 70% of CO2 in the atmosphere is absorbed by vegetation (forests, agricultural land, etc.).
Read More

“Q & A” of the european commission for labeling and desalcoholization for wines: european wine “soft-law”?

Recently, the European Commission seems to have inaugurated a new mechanism for regulating the wine sector. Through two communications, articulated in the form of “Questions & Answers”, concerning the new rules for labeling (24.11.2023) and dealcoholization of wine (15.01.2024), the Commission is not simply “explaining” the new rules but, in an approach close to the theory of “Circulaire Normative” established in comparative law, chooses among different interpretations and even adds Praeter Legem constraints.

Read More

The importance of rural extension and advisory services to achieve a sustainable viticulture in a climate change scenario

A healthy and dynamic agricultural sector is an important foundation of rural development, generating strong bonds to other economic sectors.

Read More

Preliminary steps of a protocol to isolate transcription factors bound to a specific DNA locus in grapevine using CRISPR-dCas9 system

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

Read More

Water status response of Vitis vinifera L. cv Cabernet Sauvignon during the first years within the long-term VineyardFACE (Free Air Carbon dioxide Enrichment) study 

Understanding grapevine responses to increasing atmospheric CO2 (aCO2) concentrations is crucial for assessing the impact of climate change on viticulture. Previously, at the VineyardFACE (Free Air Carbon dioxide Enrichment) experiment in Geisenheim, leaf gas exchange measurements were made as Vitis vinifera cv. Cabernet Sauvignon established from planting (2014 to 2016) under aCO2 or elevated CO2 (eCO2, aCO2 + 20%) concentrations. Contrary to many preceding observations with grapevines and other perennial plant species the young vines showed an increased intrinsic water use efficiency (WUEi) that was mainly associated with an increase in net assimilation (A) rather than a decrease in stomatal conductance (gs) under eCO2.

Read More