terclim by ICS banner
IVES 9 IVES Conference Series 9 Melatonin priming retards fungal decay in postharvest table grapes 

Melatonin priming retards fungal decay in postharvest table grapes 

Abstract

Postharvest losses of fruits may reach in some cases 40% in developed countries. This food waste has a significant carbon footprint and makes a major contribution toward greenhouse gas emissions so sustainable postharvest strategies are being investigated.

Melatonin, a well-known mammalian neurohormone, has been investigated as a priming agent to slow down fungal decay progression in postharvest climacteric and some non-climacteric fruits. However, the molecular and metabolic mechanisms responsible for such enhancement of disease tolerance are largely unknown. This work aimed to evaluate the effects of melatonin priming in fungal decay progression in postharvest table grapes (Vitis vinifera L. cv. ‘Red Globe’ and ‘Sugra 48’). Melatonin-treated grapes clearly presented lower levels of fungal decay incidence and symptom severity. DNA sequencing putatively identified three fungal species in postharvest grapes: Penicillium expansum, Penicillium crustosum and Cladosporium cladosporioides. While MDA and total anthocyanin content presented no altered levels due to melatonin treatment, phytoalexins´ profile significantly changed (e.g. trans-resveratrol, trans-piceid). Recent untargeted metabolomics data suggests that phenylpropanoid pathway is being remodelled under melatonin treatment (e.g gallic acid, catechin gallate, specific anthocyanins). RNA extraction and sequencing is being conducted to integrate these metabolic results with molecular data. Altogether, results indicate that melatonin priming leads to an effective response to fungal decay in table grapes by modulating secondary metabolism involved in defense. Ultimately, this work will clarify mechanistic processes regarding this innovative priming agent that may also have a positive impact on nutritional quality of fruits.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Article

Authors

Maria Paes 1, Florent Weiller1,  Patrícia Pardal1,  Vicent Leclère2, Inês Diniz3, Helena Gaspar1, Aziz Aziz2, Gianmaria Califano1, Ana Margarida Fortes1*

1 BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Campo Grande 1749-016, Lisbon, Portugal
2 University of Reims Champagne-Ardenne, Induced Resistance and Plant Bioprotection (RIBP), USC INRAE Reims 51100, France
3 Linking Landscape, Environment, Agriculture and Food (LEAF), TERRA—Associated Laboratory for the Sustainability of Land Use and Ecosystem Services, Instituto Superior de Agronomia, University of Lisbon, Tapada da Ajuda 1349-017 Lisbon, Portugal

Contact the author*

Keywords

melatonin priming, postharvest, table grapes, fungal decay, fruit quality

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

VOLATILE AND GLYCOSYLATED MARKERS OF SMOKE IMPACT: EVOLUTION IN BOTTLED WINE

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors. The impact on wine aroma is mainly attributed to volatile phenols, while in-mouth hydrolysis of glycosylated forms may be responsible for long-lasting “ashy” aftertastes (1).

Genomic characterization of terpene biosynthetic genes in seven Vitis vinifera L. varieties 

Grapes (Vitis vinifera L.) are a fruit crop of high economic significance globally. Each grapevine cultivar is characterized by its distinctive grape aroma, affecting the wine quality. In several cultivars, the aroma is shaped by terpenoid (mono- and sesqui-terpenoids). Their profile is controlled by terpene synthases (TPS), which are part of a largely expanded gene family. How the variation in TPS copy number and sequence among cultivars determines terpenoid profiles of grapes remains largely unexplored. We annotated TPS in the haplotypes of seven genomes (Riesling, Albariño, Fiano, Gewürztraminer, Pinot Noir, Cabernet Sauvignon, and Viognier) using BLAST, GMAP, PFAM, and phylogenetic analyses. Further, TPS expression patterns and terpenoid accumulation during berry development and ripening were characterized using RNA-Seq and SPME/GC-MS platforms, respectively. Variation in TPS copy number exists among cultivars. Specifically, the TPS counts span a range of 251 to 150 for Riesling and Fiano, respectively, when considering combined haplotypes within each cultivar. Total terpenoid accumulation patterns throughout development were consistent among the five aromatic cultivars, marked by high concentrations in flowers, followed by a decline and subsequent rise during berry development and ripening, respectively. Conversely, non-aromatic cultivars exhibited no substantial increase in terpenoid concentration during ripening. Transcriptome and network analyses are currently employed to determine which TPS are expressed in the berry and determine the terpenoid profile of the specific cultivar. These findings shed light on the genomic determinants of grape aroma in major cultivars, and allow future studies focused on cultivar-specific responses of terpenoid biosynthesis to environmental stresses.

Fining-Derived Allergens in Wine: from Detection to Quantification

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition).

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA).

“Zonation”: interpretation and estimation of “Great zonation” (GZ) following the base methodology of “GRANDE FILIERA” (GF) (Great chain)

Dans des travaux précédents sur le zonage, on a traité de la « Grande Filière », du « terroir », du « territoire », de la «″Terra »″ (« Terre »”), des « Petits zonages ou sub-zonages », du « Grand Zonage », de la qualité (nous en avons classifié plus de quatre-vingt-dix), des « Grands Objectifs » (GO) de l’activité vitivinicole et des moyens utilisés pour les atteindre. Dans le « GRAND ZONAGE » (GZ) nous avons précisé que pour zoner, nous partons des aspects